Strain Fitness in Escherichia coli ECRC100 around OKFHMN_27605

Experiment: JK42

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthdhA and uidR are separated by 228 nucleotidesuidR and lacZ are separated by 390 nucleotides OKFHMN_27600: hdhA - 7-alpha-hydroxysteroid dehydrogenase, at 5,312,751 to 5,313,518 hdhA OKFHMN_27605: uidR - DNA-binding transcriptional regulator UidR, at 5,313,747 to 5,314,337 uidR OKFHMN_27610: lacZ - Beta-galactosidase/beta-glucuronidase, at 5,314,728 to 5,315,441 lacZ Position (kb) 5313 5314 5315Strain fitness (log2 ratio) -1 0 1at 5312.816 kb on + strandat 5312.876 kb on + strand, within hdhAat 5313.002 kb on + strand, within hdhAat 5313.718 kb on + strandat 5314.236 kb on + strand, within uidRat 5314.459 kb on + strandat 5314.733 kb on - strandat 5314.914 kb on + strand, within lacZat 5315.318 kb on + strand, within lacZ

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Per-strain Table

Position Strand Gene LocusTag Fraction JK42
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5,312,816 + +0.8
5,312,876 + hdhA OKFHMN_27600 0.16 +0.5
5,313,002 + hdhA OKFHMN_27600 0.33 -0.2
5,313,718 + -0.1
5,314,236 + uidR OKFHMN_27605 0.83 -0.1
5,314,459 + +1.5
5,314,733 - -0.1
5,314,914 + lacZ OKFHMN_27610 0.26 +0.8
5,315,318 + lacZ OKFHMN_27610 0.83 +0.1

Or see this region's nucleotide sequence