Strain Fitness in Escherichia coli ECRC100 around OKFHMN_27105

Experiment: JK42

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntbtuC and btuE are separated by 62 nucleotidesbtuE and btuD overlap by 1 nucleotidesbtuD and nlpC are separated by 77 nucleotides OKFHMN_27100: btuC - vitamin B12 ABC transporter permease BtuC, at 5,215,554 to 5,216,534 btuC OKFHMN_27105: btuE - bifunctional thioredoxin/glutathione peroxidase, at 5,216,597 to 5,217,148 btuE OKFHMN_27110: btuD - vitamin B12 ABC transporter ATP-binding protein BtuD, at 5,217,148 to 5,217,897 btuD OKFHMN_27115: nlpC - putative endopeptidase NlpC, at 5,217,975 to 5,218,439 nlpC Position (kb) 5216 5217 5218Strain fitness (log2 ratio) -1 0 1at 5215.943 kb on - strand, within btuCat 5216.082 kb on + strand, within btuCat 5216.189 kb on + strand, within btuCat 5216.318 kb on + strand, within btuCat 5216.318 kb on + strand, within btuCat 5216.339 kb on + strand, within btuCat 5216.340 kb on - strand, within btuCat 5216.377 kb on + strand, within btuCat 5216.466 kb on - strandat 5216.518 kb on + strandat 5216.845 kb on + strand, within btuEat 5216.975 kb on + strand, within btuEat 5217.022 kb on + strand, within btuEat 5217.153 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction JK42
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5,215,943 - btuC OKFHMN_27100 0.40 -1.3
5,216,082 + btuC OKFHMN_27100 0.54 +0.9
5,216,189 + btuC OKFHMN_27100 0.65 -0.6
5,216,318 + btuC OKFHMN_27100 0.78 -0.7
5,216,318 + btuC OKFHMN_27100 0.78 -1.7
5,216,339 + btuC OKFHMN_27100 0.80 -0.8
5,216,340 - btuC OKFHMN_27100 0.80 -0.5
5,216,377 + btuC OKFHMN_27100 0.84 -1.0
5,216,466 - -0.5
5,216,518 + -0.4
5,216,845 + btuE OKFHMN_27105 0.45 +1.5
5,216,975 + btuE OKFHMN_27105 0.68 +0.7
5,217,022 + btuE OKFHMN_27105 0.77 +0.2
5,217,153 + +0.5

Or see this region's nucleotide sequence