Strain Fitness in Escherichia coli ECRC100 around OKFHMN_26515

Experiment: JK42

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrlmA and mntP overlap by 4 nucleotidesmntP and OKFHMN_26510 overlap by 4 nucleotidesOKFHMN_26510 and OKFHMN_26515 are separated by 310 nucleotidesOKFHMN_26515 and manZ are separated by 54 nucleotidesmanZ and manY are separated by 12 nucleotides OKFHMN_26500: rlmA - 23S rRNA (guanine(745)-N(1))-methyltransferase, at 5,101,529 to 5,102,338 rlmA OKFHMN_26505: mntP - manganese efflux pump MntP, at 5,102,335 to 5,102,901 mntP OKFHMN_26510: OKFHMN_26510 - Membrane protein, at 5,102,898 to 5,103,020 _26510 OKFHMN_26515: OKFHMN_26515 - UPF0266 membrane protein YobD, at 5,103,331 to 5,103,789 _26515 OKFHMN_26520: manZ - PTS mannose transporter subunit IID, at 5,103,844 to 5,104,695 manZ OKFHMN_26525: manY - PTS mannose transporter subunit IIC, at 5,104,708 to 5,105,508 manY Position (kb) 5103 5104Strain fitness (log2 ratio) -2 -1 0 1 2at 5102.464 kb on - strand, within mntPat 5102.866 kb on - strandat 5102.928 kb on - strand, within OKFHMN_26510at 5102.928 kb on - strand, within OKFHMN_26510at 5102.928 kb on - strand, within OKFHMN_26510at 5103.021 kb on - strandat 5103.054 kb on - strandat 5103.115 kb on - strandat 5103.539 kb on - strand, within OKFHMN_26515at 5103.835 kb on - strandat 5103.846 kb on - strandat 5103.983 kb on - strand, within manZat 5104.009 kb on - strand, within manZat 5104.209 kb on - strand, within manZat 5104.442 kb on - strand, within manZat 5104.621 kb on - strandat 5104.675 kb on - strandat 5104.686 kb on + strandat 5104.776 kb on - strandat 5104.785 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction JK42
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5,102,464 - mntP OKFHMN_26505 0.23 -1.1
5,102,866 - -1.8
5,102,928 - OKFHMN_26510 0.24 -2.0
5,102,928 - OKFHMN_26510 0.24 -1.0
5,102,928 - OKFHMN_26510 0.24 -0.1
5,103,021 - +1.6
5,103,054 - +0.4
5,103,115 - +1.4
5,103,539 - OKFHMN_26515 0.45 -0.0
5,103,835 - +0.2
5,103,846 - -0.4
5,103,983 - manZ OKFHMN_26520 0.16 -0.3
5,104,009 - manZ OKFHMN_26520 0.19 -1.0
5,104,209 - manZ OKFHMN_26520 0.43 +2.6
5,104,442 - manZ OKFHMN_26520 0.70 +1.0
5,104,621 - -1.4
5,104,675 - -1.0
5,104,686 + +0.0
5,104,776 - -1.6
5,104,785 + -0.5

Or see this region's nucleotide sequence