Strain Fitness in Escherichia coli ECRC100 around OKFHMN_25760

Experiment: JK42

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntamyA and fliT are separated by 77 nucleotidesfliT and fliS overlap by 1 nucleotidesfliS and fliD are separated by 24 nucleotides OKFHMN_25755: amyA - alpha-amylase, at 4,978,940 to 4,980,427 amyA OKFHMN_25760: fliT - flagella biosynthesis regulatory protein FliT, at 4,980,505 to 4,980,870 fliT OKFHMN_25765: fliS - flagellar export chaperone FliS, at 4,980,870 to 4,981,280 fliS OKFHMN_25770: fliD - flagellar filament capping protein FliD, at 4,981,305 to 4,982,702 fliD Position (kb) 4980 4981Strain fitness (log2 ratio) -1 0 1at 4979.597 kb on - strand, within amyAat 4979.599 kb on - strand, within amyAat 4979.645 kb on - strand, within amyAat 4979.711 kb on - strand, within amyAat 4980.007 kb on - strand, within amyAat 4980.152 kb on - strand, within amyAat 4980.203 kb on - strand, within amyAat 4980.245 kb on + strand, within amyAat 4980.295 kb on - strandat 4980.421 kb on + strandat 4980.626 kb on - strand, within fliTat 4980.629 kb on - strand, within fliTat 4980.629 kb on - strand, within fliTat 4980.637 kb on + strand, within fliTat 4980.747 kb on + strand, within fliTat 4980.930 kb on - strand, within fliSat 4981.007 kb on + strand, within fliSat 4981.008 kb on - strand, within fliSat 4981.009 kb on + strand, within fliSat 4981.014 kb on - strand, within fliSat 4981.016 kb on - strand, within fliSat 4981.039 kb on - strand, within fliSat 4981.276 kb on + strandat 4981.341 kb on + strandat 4981.342 kb on - strandat 4981.447 kb on + strand, within fliDat 4981.594 kb on - strand, within fliDat 4981.790 kb on + strand, within fliDat 4981.854 kb on + strand, within fliDat 4981.854 kb on + strand, within fliDat 4981.868 kb on - strand, within fliDat 4981.868 kb on - strand, within fliD

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Per-strain Table

Position Strand Gene LocusTag Fraction JK42
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4,979,597 - amyA OKFHMN_25755 0.44 +0.7
4,979,599 - amyA OKFHMN_25755 0.44 +1.6
4,979,645 - amyA OKFHMN_25755 0.47 +0.5
4,979,711 - amyA OKFHMN_25755 0.52 +1.0
4,980,007 - amyA OKFHMN_25755 0.72 +0.8
4,980,152 - amyA OKFHMN_25755 0.81 +0.8
4,980,203 - amyA OKFHMN_25755 0.85 -0.5
4,980,245 + amyA OKFHMN_25755 0.88 +0.0
4,980,295 - -1.6
4,980,421 + +1.2
4,980,626 - fliT OKFHMN_25760 0.33 +0.4
4,980,629 - fliT OKFHMN_25760 0.34 -0.3
4,980,629 - fliT OKFHMN_25760 0.34 +0.5
4,980,637 + fliT OKFHMN_25760 0.36 +0.2
4,980,747 + fliT OKFHMN_25760 0.66 -0.2
4,980,930 - fliS OKFHMN_25765 0.15 -0.3
4,981,007 + fliS OKFHMN_25765 0.33 -1.2
4,981,008 - fliS OKFHMN_25765 0.34 -0.7
4,981,009 + fliS OKFHMN_25765 0.34 -0.6
4,981,014 - fliS OKFHMN_25765 0.35 +0.2
4,981,016 - fliS OKFHMN_25765 0.36 -0.2
4,981,039 - fliS OKFHMN_25765 0.41 -0.4
4,981,276 + +0.8
4,981,341 + +0.6
4,981,342 - -0.4
4,981,447 + fliD OKFHMN_25770 0.10 -0.1
4,981,594 - fliD OKFHMN_25770 0.21 -1.7
4,981,790 + fliD OKFHMN_25770 0.35 -1.0
4,981,854 + fliD OKFHMN_25770 0.39 +1.6
4,981,854 + fliD OKFHMN_25770 0.39 +1.1
4,981,868 - fliD OKFHMN_25770 0.40 -0.3
4,981,868 - fliD OKFHMN_25770 0.40 +0.6

Or see this region's nucleotide sequence