Strain Fitness in Escherichia coli ECRC100 around OKFHMN_25325

Experiment: JK42

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntOKFHMN_25320 and rusA are separated by 12 nucleotidesrusA and OKFHMN_25330 overlap by 4 nucleotidesOKFHMN_25330 and Ile2_trna are separated by 210 nucleotidesIle2_trna and Arg_trna are separated by 7 nucleotides OKFHMN_25320: OKFHMN_25320 - DUF968 domain-containing protein, at 4,910,802 to 4,911,851 _25320 OKFHMN_25325: rusA - Endodeoxyribonuclease RusA, at 4,911,864 to 4,912,223 rusA OKFHMN_25330: OKFHMN_25330 - antiterminator, at 4,912,220 to 4,912,909 _25330 OKFHMN_25335: Ile2_trna - tRNA-Ile2(cat), at 4,913,120 to 4,913,195 _trna OKFHMN_25340: Arg_trna - tRNA-Arg(tcg), at 4,913,203 to 4,913,279 _trna Position (kb) 4911 4912 4913Strain fitness (log2 ratio) -1 0 1 2at 4910.900 kb on - strandat 4911.071 kb on + strand, within OKFHMN_25320at 4911.072 kb on - strand, within OKFHMN_25320at 4911.283 kb on + strand, within OKFHMN_25320at 4911.415 kb on + strand, within OKFHMN_25320at 4911.416 kb on - strand, within OKFHMN_25320at 4911.442 kb on + strand, within OKFHMN_25320at 4911.573 kb on - strand, within OKFHMN_25320at 4912.232 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction JK42
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4,910,900 - +0.5
4,911,071 + OKFHMN_25320 0.26 +0.0
4,911,072 - OKFHMN_25320 0.26 +2.6
4,911,283 + OKFHMN_25320 0.46 -0.7
4,911,415 + OKFHMN_25320 0.58 +0.4
4,911,416 - OKFHMN_25320 0.58 -0.0
4,911,442 + OKFHMN_25320 0.61 -0.5
4,911,573 - OKFHMN_25320 0.73 +0.3
4,912,232 + -0.2

Or see this region's nucleotide sequence