Strain Fitness in Escherichia coli ECRC100 around OKFHMN_25210

Experiment: JK42

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntOKFHMN_25195 and OKFHMN_25200 are separated by 13 nucleotidesOKFHMN_25200 and Asn_trna are separated by 123 nucleotidesAsn_trna and mtfA are separated by 100 nucleotidesmtfA and Ser_trna are separated by 93 nucleotidesSer_trna and xerD are separated by 52 nucleotides OKFHMN_25195: OKFHMN_25195 - Ig-like domain-containing protein, at 4,889,638 to 4,897,647 _25195 OKFHMN_25200: OKFHMN_25200 - hypothetical protein, at 4,897,661 to 4,897,810 _25200 OKFHMN_25205: Asn_trna - tRNA-Asn(gtt), at 4,897,934 to 4,898,009 _trna OKFHMN_25210: mtfA - DgsA anti-repressor MtfA, at 4,898,110 to 4,898,907 mtfA OKFHMN_25215: Ser_trna - tRNA-Ser(cga), at 4,899,001 to 4,899,090 _trna OKFHMN_25220: xerD - integrase, at 4,899,143 to 4,900,165 xerD Position (kb) 4898 4899Strain fitness (log2 ratio) -1 0 1at 4897.409 kb on + strandat 4897.520 kb on - strandat 4897.624 kb on + strandat 4897.848 kb on - strandat 4897.856 kb on - strandat 4898.297 kb on + strand, within mtfAat 4898.297 kb on + strand, within mtfAat 4898.695 kb on + strand, within mtfAat 4898.884 kb on + strandat 4898.901 kb on + strandat 4898.901 kb on + strandat 4898.901 kb on + strandat 4898.902 kb on - strandat 4898.961 kb on - strandat 4899.225 kb on - strandat 4899.286 kb on - strand, within xerDat 4899.397 kb on - strand, within xerDat 4899.400 kb on + strand, within xerDat 4899.401 kb on - strand, within xerDat 4899.401 kb on - strand, within xerDat 4899.401 kb on - strand, within xerDat 4899.645 kb on - strand, within xerDat 4899.671 kb on + strand, within xerDat 4899.736 kb on - strand, within xerDat 4899.829 kb on - strand, within xerD

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Per-strain Table

Position Strand Gene LocusTag Fraction JK42
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4,897,409 + -0.7
4,897,520 - +1.6
4,897,624 + +0.6
4,897,848 - +0.6
4,897,856 - +0.4
4,898,297 + mtfA OKFHMN_25210 0.23 +1.6
4,898,297 + mtfA OKFHMN_25210 0.23 +0.2
4,898,695 + mtfA OKFHMN_25210 0.73 +0.3
4,898,884 + +0.0
4,898,901 + -0.4
4,898,901 + -0.6
4,898,901 + +1.6
4,898,902 - -0.5
4,898,961 - +0.1
4,899,225 - -0.7
4,899,286 - xerD OKFHMN_25220 0.14 -0.2
4,899,397 - xerD OKFHMN_25220 0.25 +0.4
4,899,400 + xerD OKFHMN_25220 0.25 +0.6
4,899,401 - xerD OKFHMN_25220 0.25 -1.6
4,899,401 - xerD OKFHMN_25220 0.25 +0.3
4,899,401 - xerD OKFHMN_25220 0.25 +1.6
4,899,645 - xerD OKFHMN_25220 0.49 +0.0
4,899,671 + xerD OKFHMN_25220 0.52 -0.5
4,899,736 - xerD OKFHMN_25220 0.58 +1.6
4,899,829 - xerD OKFHMN_25220 0.67 +1.6

Or see this region's nucleotide sequence