Strain Fitness in Escherichia coli ECRC100 around OKFHMN_24435

Experiment: JK42

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrfbE and rfaB are separated by 24 nucleotidesrfaB and OKFHMN_24440 are separated by 19 nucleotides OKFHMN_24430: rfbE - GDP-perosamine synthase RfbE/PerA, at 4,772,579 to 4,773,673 rfbE OKFHMN_24435: rfaB - glycosyl transferase, at 4,773,698 to 4,774,912 rfaB OKFHMN_24440: OKFHMN_24440 - GDP-mannose 4,6-dehydratase, at 4,774,932 to 4,776,050 _24440 Position (kb) 4773 4774 4775Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 4772.827 kb on + strand, within rfbEat 4772.829 kb on + strand, within rfbEat 4773.247 kb on - strand, within rfbEat 4773.285 kb on + strand, within rfbEat 4774.573 kb on + strand, within rfaBat 4775.479 kb on + strand, within OKFHMN_24440at 4775.747 kb on + strand, within OKFHMN_24440

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Per-strain Table

Position Strand Gene LocusTag Fraction JK42
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4,772,827 + rfbE OKFHMN_24430 0.23 -3.9
4,772,829 + rfbE OKFHMN_24430 0.23 -2.0
4,773,247 - rfbE OKFHMN_24430 0.61 -3.7
4,773,285 + rfbE OKFHMN_24430 0.64 -3.7
4,774,573 + rfaB OKFHMN_24435 0.72 -2.9
4,775,479 + OKFHMN_24440 0.49 -1.0
4,775,747 + OKFHMN_24440 0.73 +0.0

Or see this region's nucleotide sequence