Strain Fitness in Escherichia coli ECRC100 around OKFHMN_22105

Experiment: JK42

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntamiA and ypeA are separated by 213 nucleotidesypeA and yfeZ overlap by 14 nucleotidesyfeZ and yfeY are separated by 60 nucleotides OKFHMN_22100: amiA - N-acetylmuramoyl-L-alanine amidase AmiA, at 4,298,255 to 4,299,124 amiA OKFHMN_22105: ypeA - GNAT family acetyltransferase, at 4,299,338 to 4,299,763 ypeA OKFHMN_22110: yfeZ - Inner membrane protein YfeZ, at 4,299,750 to 4,300,199 yfeZ OKFHMN_22115: yfeY - RpoE-regulated lipoprotein, at 4,300,260 to 4,300,835 yfeY Position (kb) 4299 4300Strain fitness (log2 ratio) -1 0 1 2at 4298.530 kb on + strand, within amiAat 4298.714 kb on - strand, within amiAat 4298.837 kb on - strand, within amiAat 4299.009 kb on - strand, within amiAat 4299.138 kb on + strandat 4299.159 kb on + strandat 4299.160 kb on - strandat 4299.160 kb on - strandat 4299.162 kb on - strandat 4299.173 kb on + strandat 4299.210 kb on - strandat 4299.225 kb on - strandat 4299.334 kb on + strandat 4299.351 kb on + strandat 4299.445 kb on + strand, within ypeAat 4299.446 kb on - strand, within ypeAat 4299.548 kb on + strand, within ypeAat 4299.549 kb on - strand, within ypeAat 4299.549 kb on - strand, within ypeAat 4299.549 kb on - strand, within ypeAat 4299.805 kb on + strand, within yfeZat 4299.890 kb on + strand, within yfeZat 4299.912 kb on + strand, within yfeZat 4299.995 kb on + strand, within yfeZat 4300.023 kb on + strand, within yfeZat 4300.054 kb on + strand, within yfeZat 4300.055 kb on - strand, within yfeZat 4300.120 kb on + strand, within yfeZat 4300.276 kb on + strandat 4300.276 kb on + strandat 4300.289 kb on + strandat 4300.332 kb on - strand, within yfeYat 4300.406 kb on - strand, within yfeYat 4300.691 kb on + strand, within yfeYat 4300.730 kb on + strand, within yfeYat 4300.731 kb on - strand, within yfeYat 4300.733 kb on + strand, within yfeYat 4300.733 kb on + strand, within yfeYat 4300.733 kb on + strand, within yfeYat 4300.734 kb on - strand, within yfeYat 4300.734 kb on - strand, within yfeY

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Per-strain Table

Position Strand Gene LocusTag Fraction JK42
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4,298,530 + amiA OKFHMN_22100 0.32 -0.9
4,298,714 - amiA OKFHMN_22100 0.53 -0.1
4,298,837 - amiA OKFHMN_22100 0.67 -0.5
4,299,009 - amiA OKFHMN_22100 0.87 +0.4
4,299,138 + +0.9
4,299,159 + +1.3
4,299,160 - -0.2
4,299,160 - +0.1
4,299,162 - -0.2
4,299,173 + +0.8
4,299,210 - -0.3
4,299,225 - +0.5
4,299,334 + -0.2
4,299,351 + -0.4
4,299,445 + ypeA OKFHMN_22105 0.25 -1.3
4,299,446 - ypeA OKFHMN_22105 0.25 +0.2
4,299,548 + ypeA OKFHMN_22105 0.49 +2.3
4,299,549 - ypeA OKFHMN_22105 0.50 +0.1
4,299,549 - ypeA OKFHMN_22105 0.50 -0.7
4,299,549 - ypeA OKFHMN_22105 0.50 +0.9
4,299,805 + yfeZ OKFHMN_22110 0.12 +0.8
4,299,890 + yfeZ OKFHMN_22110 0.31 +1.1
4,299,912 + yfeZ OKFHMN_22110 0.36 -0.6
4,299,995 + yfeZ OKFHMN_22110 0.54 -0.8
4,300,023 + yfeZ OKFHMN_22110 0.61 -0.2
4,300,054 + yfeZ OKFHMN_22110 0.68 -0.8
4,300,055 - yfeZ OKFHMN_22110 0.68 +0.7
4,300,120 + yfeZ OKFHMN_22110 0.82 -0.2
4,300,276 + +0.1
4,300,276 + -1.0
4,300,289 + -0.1
4,300,332 - yfeY OKFHMN_22115 0.12 -0.7
4,300,406 - yfeY OKFHMN_22115 0.25 +0.3
4,300,691 + yfeY OKFHMN_22115 0.75 +0.3
4,300,730 + yfeY OKFHMN_22115 0.82 -0.1
4,300,731 - yfeY OKFHMN_22115 0.82 -1.0
4,300,733 + yfeY OKFHMN_22115 0.82 +0.1
4,300,733 + yfeY OKFHMN_22115 0.82 +0.8
4,300,733 + yfeY OKFHMN_22115 0.82 +0.6
4,300,734 - yfeY OKFHMN_22115 0.82 +0.2
4,300,734 - yfeY OKFHMN_22115 0.82 +2.3

Or see this region's nucleotide sequence