Experiment: JK42
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dapE and yffB are separated by 3 nucleotides yffB and ypfM are separated by 380 nucleotides ypfM and acrD are separated by 98 nucleotides
OKFHMN_21955: dapE - succinyl-diaminopimelate desuccinylase, at 4,265,721 to 4,266,848
dapE
OKFHMN_21960: yffB - ArsC family reductase, at 4,266,852 to 4,267,208
yffB
OKFHMN_21965: ypfM - protein YpfM, at 4,267,589 to 4,267,648
ypfM
OKFHMN_21970: acrD - multidrug efflux RND transporter permease AcrD, at 4,267,747 to 4,270,860
acrD
Position (kb)
4267
4268 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4266.906 kb on - strand, within yffB at 4266.906 kb on - strand, within yffB at 4266.996 kb on - strand, within yffB at 4267.020 kb on - strand, within yffB at 4267.031 kb on + strand, within yffB at 4267.087 kb on - strand, within yffB at 4267.196 kb on - strand at 4267.196 kb on - strand at 4267.289 kb on + strand at 4267.290 kb on - strand at 4267.305 kb on + strand at 4267.315 kb on - strand at 4267.321 kb on + strand at 4267.324 kb on + strand at 4267.407 kb on + strand at 4267.420 kb on - strand at 4267.426 kb on - strand at 4267.452 kb on + strand at 4267.453 kb on - strand at 4267.476 kb on - strand at 4267.478 kb on + strand at 4267.478 kb on + strand at 4267.478 kb on + strand at 4267.478 kb on + strand at 4267.479 kb on - strand at 4267.482 kb on + strand at 4267.483 kb on - strand at 4267.507 kb on - strand at 4267.527 kb on - strand at 4267.531 kb on + strand at 4267.546 kb on - strand at 4267.546 kb on - strand at 4267.547 kb on + strand at 4267.547 kb on + strand at 4267.548 kb on - strand at 4267.548 kb on - strand at 4267.548 kb on - strand at 4267.563 kb on + strand at 4267.564 kb on - strand at 4267.569 kb on + strand at 4267.570 kb on - strand at 4267.573 kb on + strand at 4267.574 kb on - strand at 4267.574 kb on - strand at 4267.748 kb on + strand at 4267.749 kb on - strand at 4267.811 kb on + strand at 4267.824 kb on + strand at 4267.825 kb on - strand at 4268.193 kb on - strand, within acrD at 4268.216 kb on - strand, within acrD at 4268.340 kb on + strand, within acrD at 4268.412 kb on - strand, within acrD at 4268.487 kb on + strand, within acrD at 4268.553 kb on - strand, within acrD at 4268.594 kb on + strand, within acrD at 4268.594 kb on + strand, within acrD at 4268.594 kb on + strand, within acrD at 4268.628 kb on + strand, within acrD
Per-strain Table
Position Strand Gene LocusTag Fraction JK42 remove 4,266,906 - yffB OKFHMN_21960 0.15 -0.4 4,266,906 - yffB OKFHMN_21960 0.15 -0.3 4,266,996 - yffB OKFHMN_21960 0.40 +0.5 4,267,020 - yffB OKFHMN_21960 0.47 -0.4 4,267,031 + yffB OKFHMN_21960 0.50 -0.2 4,267,087 - yffB OKFHMN_21960 0.66 +0.3 4,267,196 - -0.6 4,267,196 - -1.5 4,267,289 + +1.7 4,267,290 - +0.4 4,267,305 + +0.1 4,267,315 - +0.2 4,267,321 + -1.0 4,267,324 + +0.4 4,267,407 + +0.7 4,267,420 - +0.2 4,267,426 - +0.3 4,267,452 + -0.5 4,267,453 - -0.1 4,267,476 - +0.8 4,267,478 + -0.4 4,267,478 + -0.1 4,267,478 + +0.5 4,267,478 + -0.4 4,267,479 - +1.1 4,267,482 + -0.6 4,267,483 - +0.3 4,267,507 - +0.4 4,267,527 - -0.1 4,267,531 + -0.4 4,267,546 - +0.2 4,267,546 - +1.5 4,267,547 + -0.1 4,267,547 + +0.9 4,267,548 - +0.5 4,267,548 - -2.2 4,267,548 - -0.6 4,267,563 + +1.3 4,267,564 - +0.2 4,267,569 + +0.7 4,267,570 - -0.7 4,267,573 + -1.5 4,267,574 - -0.5 4,267,574 - +1.1 4,267,748 + +0.7 4,267,749 - -1.1 4,267,811 + +0.3 4,267,824 + -0.1 4,267,825 - +2.7 4,268,193 - acrD OKFHMN_21970 0.14 +0.1 4,268,216 - acrD OKFHMN_21970 0.15 +0.1 4,268,340 + acrD OKFHMN_21970 0.19 +0.5 4,268,412 - acrD OKFHMN_21970 0.21 -0.5 4,268,487 + acrD OKFHMN_21970 0.24 +0.7 4,268,553 - acrD OKFHMN_21970 0.26 -0.1 4,268,594 + acrD OKFHMN_21970 0.27 -1.2 4,268,594 + acrD OKFHMN_21970 0.27 +0.4 4,268,594 + acrD OKFHMN_21970 0.27 -0.3 4,268,628 + acrD OKFHMN_21970 0.28 +0.4
Or see this region's nucleotide sequence