Strain Fitness in Escherichia coli ECRC100 around OKFHMN_20610

Experiment: JK42

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthycG and hycH overlap by 4 nucleotideshycH and hycI overlap by 8 nucleotideshycI and ascB are separated by 123 nucleotides OKFHMN_20600: hycG - formate hydrogenlyase subunit HycG, at 4,008,496 to 4,009,263 hycG OKFHMN_20605: hycH - formate hydrogenlyase assembly protein HycH, at 4,009,260 to 4,009,670 hycH OKFHMN_20610: hycI - hydrogenase maturation peptidase HycI, at 4,009,663 to 4,010,133 hycI OKFHMN_20615: ascB - 6-phospho-beta-glucosidase AscB, at 4,010,257 to 4,011,681 ascB Position (kb) 4009 4010 4011Strain fitness (log2 ratio) -2 -1 0 1 2at 4008.741 kb on - strand, within hycGat 4008.830 kb on - strand, within hycGat 4008.830 kb on - strand, within hycGat 4008.916 kb on + strand, within hycGat 4008.916 kb on + strand, within hycGat 4008.917 kb on - strand, within hycGat 4008.917 kb on - strand, within hycGat 4009.138 kb on + strand, within hycGat 4009.138 kb on + strand, within hycGat 4009.138 kb on + strand, within hycGat 4009.144 kb on - strand, within hycGat 4009.152 kb on - strand, within hycGat 4009.254 kb on + strandat 4009.347 kb on + strand, within hycHat 4009.348 kb on - strand, within hycHat 4009.350 kb on + strand, within hycHat 4009.494 kb on + strand, within hycHat 4009.494 kb on + strand, within hycHat 4009.592 kb on + strand, within hycHat 4009.603 kb on + strand, within hycHat 4009.604 kb on - strand, within hycHat 4009.607 kb on + strand, within hycHat 4009.620 kb on - strand, within hycHat 4009.641 kb on + strandat 4009.641 kb on + strandat 4009.641 kb on + strandat 4009.676 kb on + strandat 4009.759 kb on - strand, within hycIat 4009.933 kb on - strand, within hycIat 4009.933 kb on - strand, within hycIat 4009.974 kb on + strand, within hycIat 4009.975 kb on - strand, within hycIat 4010.146 kb on + strandat 4010.146 kb on + strandat 4010.147 kb on - strandat 4010.255 kb on - strandat 4010.259 kb on - strandat 4010.298 kb on - strandat 4010.316 kb on + strandat 4010.317 kb on - strandat 4010.317 kb on - strandat 4010.376 kb on - strandat 4010.435 kb on + strand, within ascBat 4010.499 kb on - strand, within ascBat 4010.600 kb on + strand, within ascBat 4010.600 kb on + strand, within ascBat 4010.600 kb on + strand, within ascBat 4010.600 kb on + strand, within ascBat 4010.601 kb on - strand, within ascBat 4010.601 kb on - strand, within ascBat 4010.601 kb on - strand, within ascBat 4010.601 kb on - strand, within ascBat 4010.601 kb on - strand, within ascBat 4010.601 kb on - strand, within ascBat 4010.620 kb on - strand, within ascBat 4010.665 kb on + strand, within ascBat 4010.666 kb on - strand, within ascBat 4010.666 kb on - strand, within ascBat 4010.666 kb on - strand, within ascBat 4010.666 kb on - strand, within ascBat 4010.693 kb on - strand, within ascBat 4010.751 kb on - strand, within ascBat 4010.751 kb on - strand, within ascBat 4010.753 kb on + strand, within ascBat 4010.954 kb on + strand, within ascBat 4010.955 kb on - strand, within ascBat 4011.062 kb on + strand, within ascBat 4011.062 kb on + strand, within ascBat 4011.063 kb on - strand, within ascB

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Per-strain Table

Position Strand Gene LocusTag Fraction JK42
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4,008,741 - hycG OKFHMN_20600 0.32 +0.8
4,008,830 - hycG OKFHMN_20600 0.43 +0.2
4,008,830 - hycG OKFHMN_20600 0.43 +0.7
4,008,916 + hycG OKFHMN_20600 0.55 -1.1
4,008,916 + hycG OKFHMN_20600 0.55 -0.0
4,008,917 - hycG OKFHMN_20600 0.55 +0.3
4,008,917 - hycG OKFHMN_20600 0.55 -0.5
4,009,138 + hycG OKFHMN_20600 0.84 -0.0
4,009,138 + hycG OKFHMN_20600 0.84 -0.6
4,009,138 + hycG OKFHMN_20600 0.84 +0.2
4,009,144 - hycG OKFHMN_20600 0.84 -0.7
4,009,152 - hycG OKFHMN_20600 0.85 -1.0
4,009,254 + -1.0
4,009,347 + hycH OKFHMN_20605 0.21 -0.3
4,009,348 - hycH OKFHMN_20605 0.21 -0.3
4,009,350 + hycH OKFHMN_20605 0.22 -0.1
4,009,494 + hycH OKFHMN_20605 0.57 -0.0
4,009,494 + hycH OKFHMN_20605 0.57 +0.0
4,009,592 + hycH OKFHMN_20605 0.81 +0.1
4,009,603 + hycH OKFHMN_20605 0.83 +0.2
4,009,604 - hycH OKFHMN_20605 0.84 +0.7
4,009,607 + hycH OKFHMN_20605 0.84 +0.4
4,009,620 - hycH OKFHMN_20605 0.88 +0.2
4,009,641 + -1.2
4,009,641 + -0.2
4,009,641 + +0.0
4,009,676 + +0.3
4,009,759 - hycI OKFHMN_20610 0.20 -0.3
4,009,933 - hycI OKFHMN_20610 0.57 -2.0
4,009,933 - hycI OKFHMN_20610 0.57 +0.5
4,009,974 + hycI OKFHMN_20610 0.66 -0.4
4,009,975 - hycI OKFHMN_20610 0.66 -1.8
4,010,146 + -0.5
4,010,146 + +0.5
4,010,147 - -0.5
4,010,255 - -0.1
4,010,259 - +0.8
4,010,298 - -0.5
4,010,316 + +0.3
4,010,317 - +0.4
4,010,317 - +0.5
4,010,376 - +0.1
4,010,435 + ascB OKFHMN_20615 0.12 +1.0
4,010,499 - ascB OKFHMN_20615 0.17 +0.0
4,010,600 + ascB OKFHMN_20615 0.24 -1.0
4,010,600 + ascB OKFHMN_20615 0.24 -0.4
4,010,600 + ascB OKFHMN_20615 0.24 -0.3
4,010,600 + ascB OKFHMN_20615 0.24 +0.5
4,010,601 - ascB OKFHMN_20615 0.24 +0.3
4,010,601 - ascB OKFHMN_20615 0.24 -0.2
4,010,601 - ascB OKFHMN_20615 0.24 +0.9
4,010,601 - ascB OKFHMN_20615 0.24 -0.6
4,010,601 - ascB OKFHMN_20615 0.24 -0.5
4,010,601 - ascB OKFHMN_20615 0.24 -0.2
4,010,620 - ascB OKFHMN_20615 0.25 -0.2
4,010,665 + ascB OKFHMN_20615 0.29 -1.1
4,010,666 - ascB OKFHMN_20615 0.29 +0.6
4,010,666 - ascB OKFHMN_20615 0.29 -0.4
4,010,666 - ascB OKFHMN_20615 0.29 +0.8
4,010,666 - ascB OKFHMN_20615 0.29 -0.1
4,010,693 - ascB OKFHMN_20615 0.31 -1.7
4,010,751 - ascB OKFHMN_20615 0.35 +0.2
4,010,751 - ascB OKFHMN_20615 0.35 +0.8
4,010,753 + ascB OKFHMN_20615 0.35 -0.2
4,010,954 + ascB OKFHMN_20615 0.49 -0.5
4,010,955 - ascB OKFHMN_20615 0.49 -0.2
4,011,062 + ascB OKFHMN_20615 0.56 +1.8
4,011,062 + ascB OKFHMN_20615 0.56 +1.4
4,011,063 - ascB OKFHMN_20615 0.57 -0.9

Or see this region's nucleotide sequence