Experiment: JK42
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cas3 and casA are separated by 97 nucleotides casA and casB overlap by 4 nucleotides casB and cas7e are separated by 11 nucleotides
OKFHMN_20395: cas3 - CRISPR-associated helicase/endonuclease Cas3, at 3,969,300 to 3,971,999
cas3
OKFHMN_20400: casA - type I-E CRISPR-associated protein Cse1/CasA, at 3,972,097 to 3,973,659
casA
OKFHMN_20405: casB - type I-E CRISPR-associated protein Cse2/CasB, at 3,973,656 to 3,974,192
casB
OKFHMN_20410: cas7e - type I-E CRISPR-associated protein Cas7/Cse4/CasC, at 3,974,204 to 3,975,259
cas7e
Position (kb)
3972
3973
3974 Strain fitness (log2 ratio)
-1
0
1
2 at 3971.207 kb on + strand, within cas3 at 3971.324 kb on + strand, within cas3 at 3971.324 kb on + strand, within cas3 at 3971.324 kb on + strand, within cas3 at 3971.325 kb on - strand, within cas3 at 3971.439 kb on - strand, within cas3 at 3971.448 kb on + strand, within cas3 at 3971.448 kb on + strand, within cas3 at 3971.449 kb on - strand, within cas3 at 3971.449 kb on - strand, within cas3 at 3971.449 kb on - strand, within cas3 at 3971.449 kb on - strand, within cas3 at 3971.449 kb on - strand, within cas3 at 3971.449 kb on - strand, within cas3 at 3971.449 kb on - strand, within cas3 at 3971.491 kb on + strand, within cas3 at 3971.540 kb on + strand, within cas3 at 3971.553 kb on + strand, within cas3 at 3971.569 kb on + strand, within cas3 at 3971.570 kb on - strand, within cas3 at 3971.779 kb on - strand at 3972.002 kb on + strand at 3972.003 kb on - strand at 3972.092 kb on + strand at 3972.096 kb on + strand at 3972.096 kb on + strand at 3972.097 kb on - strand at 3972.260 kb on + strand, within casA at 3972.263 kb on + strand, within casA at 3972.263 kb on + strand, within casA at 3972.264 kb on - strand, within casA at 3972.306 kb on + strand, within casA at 3972.307 kb on - strand, within casA at 3972.359 kb on + strand, within casA at 3972.359 kb on + strand, within casA at 3972.486 kb on + strand, within casA at 3972.527 kb on + strand, within casA at 3972.527 kb on + strand, within casA at 3972.528 kb on - strand, within casA at 3972.563 kb on + strand, within casA at 3972.569 kb on + strand, within casA at 3972.570 kb on - strand, within casA at 3972.652 kb on + strand, within casA at 3972.652 kb on + strand, within casA at 3972.653 kb on - strand, within casA at 3972.725 kb on + strand, within casA at 3972.964 kb on + strand, within casA at 3972.995 kb on - strand, within casA at 3973.001 kb on + strand, within casA at 3973.222 kb on + strand, within casA at 3973.431 kb on - strand, within casA at 3973.530 kb on - strand at 3973.661 kb on + strand at 3973.666 kb on + strand at 3973.667 kb on - strand at 3973.751 kb on + strand, within casB at 3973.813 kb on + strand, within casB at 3973.813 kb on + strand, within casB at 3973.814 kb on - strand, within casB at 3973.842 kb on - strand, within casB at 3974.005 kb on + strand, within casB at 3974.014 kb on + strand, within casB at 3974.014 kb on + strand, within casB at 3974.127 kb on - strand, within casB at 3974.152 kb on - strand at 3974.152 kb on - strand at 3974.173 kb on - strand at 3974.387 kb on - strand, within cas7e at 3974.407 kb on + strand, within cas7e at 3974.428 kb on + strand, within cas7e at 3974.429 kb on - strand, within cas7e at 3974.559 kb on + strand, within cas7e at 3974.559 kb on + strand, within cas7e at 3974.566 kb on + strand, within cas7e at 3974.592 kb on + strand, within cas7e
Per-strain Table
Position Strand Gene LocusTag Fraction JK42 remove 3,971,207 + cas3 OKFHMN_20395 0.71 -0.6 3,971,324 + cas3 OKFHMN_20395 0.75 -0.5 3,971,324 + cas3 OKFHMN_20395 0.75 -0.0 3,971,324 + cas3 OKFHMN_20395 0.75 +1.6 3,971,325 - cas3 OKFHMN_20395 0.75 -0.5 3,971,439 - cas3 OKFHMN_20395 0.79 +1.2 3,971,448 + cas3 OKFHMN_20395 0.80 -0.1 3,971,448 + cas3 OKFHMN_20395 0.80 +1.0 3,971,449 - cas3 OKFHMN_20395 0.80 +1.8 3,971,449 - cas3 OKFHMN_20395 0.80 +0.4 3,971,449 - cas3 OKFHMN_20395 0.80 -0.4 3,971,449 - cas3 OKFHMN_20395 0.80 -0.4 3,971,449 - cas3 OKFHMN_20395 0.80 -1.5 3,971,449 - cas3 OKFHMN_20395 0.80 +1.8 3,971,449 - cas3 OKFHMN_20395 0.80 +1.0 3,971,491 + cas3 OKFHMN_20395 0.81 +0.5 3,971,540 + cas3 OKFHMN_20395 0.83 +0.0 3,971,553 + cas3 OKFHMN_20395 0.83 +0.2 3,971,569 + cas3 OKFHMN_20395 0.84 -1.0 3,971,570 - cas3 OKFHMN_20395 0.84 +0.8 3,971,779 - -0.2 3,972,002 + +0.2 3,972,003 - +0.8 3,972,092 + -0.2 3,972,096 + -0.1 3,972,096 + -0.0 3,972,097 - +0.3 3,972,260 + casA OKFHMN_20400 0.10 -0.3 3,972,263 + casA OKFHMN_20400 0.11 +0.1 3,972,263 + casA OKFHMN_20400 0.11 +2.4 3,972,264 - casA OKFHMN_20400 0.11 +0.9 3,972,306 + casA OKFHMN_20400 0.13 -0.4 3,972,307 - casA OKFHMN_20400 0.13 -0.0 3,972,359 + casA OKFHMN_20400 0.17 -1.6 3,972,359 + casA OKFHMN_20400 0.17 -1.1 3,972,486 + casA OKFHMN_20400 0.25 +0.2 3,972,527 + casA OKFHMN_20400 0.28 -0.9 3,972,527 + casA OKFHMN_20400 0.28 +0.5 3,972,528 - casA OKFHMN_20400 0.28 -0.1 3,972,563 + casA OKFHMN_20400 0.30 -0.6 3,972,569 + casA OKFHMN_20400 0.30 +0.3 3,972,570 - casA OKFHMN_20400 0.30 +0.0 3,972,652 + casA OKFHMN_20400 0.36 +0.2 3,972,652 + casA OKFHMN_20400 0.36 +0.8 3,972,653 - casA OKFHMN_20400 0.36 +0.6 3,972,725 + casA OKFHMN_20400 0.40 +0.6 3,972,964 + casA OKFHMN_20400 0.55 +0.5 3,972,995 - casA OKFHMN_20400 0.57 -0.3 3,973,001 + casA OKFHMN_20400 0.58 -1.5 3,973,222 + casA OKFHMN_20400 0.72 -0.2 3,973,431 - casA OKFHMN_20400 0.85 +0.8 3,973,530 - +1.4 3,973,661 + +0.5 3,973,666 + -0.3 3,973,667 - +0.2 3,973,751 + casB OKFHMN_20405 0.18 -0.8 3,973,813 + casB OKFHMN_20405 0.29 -0.4 3,973,813 + casB OKFHMN_20405 0.29 +0.0 3,973,814 - casB OKFHMN_20405 0.29 +1.0 3,973,842 - casB OKFHMN_20405 0.35 -0.2 3,974,005 + casB OKFHMN_20405 0.65 -1.0 3,974,014 + casB OKFHMN_20405 0.67 +0.8 3,974,014 + casB OKFHMN_20405 0.67 -0.4 3,974,127 - casB OKFHMN_20405 0.88 +0.3 3,974,152 - -0.4 3,974,152 - -0.1 3,974,173 - +0.3 3,974,387 - cas7e OKFHMN_20410 0.17 +0.3 3,974,407 + cas7e OKFHMN_20410 0.19 -0.9 3,974,428 + cas7e OKFHMN_20410 0.21 +0.6 3,974,429 - cas7e OKFHMN_20410 0.21 +0.6 3,974,559 + cas7e OKFHMN_20410 0.34 +0.2 3,974,559 + cas7e OKFHMN_20410 0.34 +0.4 3,974,566 + cas7e OKFHMN_20410 0.34 +0.1 3,974,592 + cas7e OKFHMN_20410 0.37 +0.6
Or see this region's nucleotide sequence