Strain Fitness in Escherichia coli ECRC100 around OKFHMN_15335

Experiment: JK42

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntescC and sepD overlap by 1 nucleotidessepD and escJ are separated by 5 nucleotidesescJ and escI are separated by 2 nucleotides OKFHMN_15330: escC - type III secretion system LEE outer membrane ring protein EscC, at 2,962,165 to 2,963,703 escC OKFHMN_15335: sepD - type III secretion system LEE switch protein SepD, at 2,963,703 to 2,964,158 sepD OKFHMN_15340: escJ - type III secretion system LEE inner membrane ring protein EscJ, at 2,964,164 to 2,964,736 escJ OKFHMN_15345: escI - Type III secretion system inner rod component EscI, at 2,964,739 to 2,965,116 escI Position (kb) 2963 2964 2965Strain fitness (log2 ratio) -2 -1 0 1at 2962.771 kb on - strand, within escCat 2962.858 kb on - strand, within escCat 2963.127 kb on + strand, within escCat 2963.127 kb on + strand, within escCat 2963.446 kb on + strand, within escCat 2963.775 kb on + strand, within sepDat 2964.096 kb on + strand, within sepDat 2964.139 kb on - strandat 2964.158 kb on + strandat 2964.272 kb on + strand, within escJat 2964.721 kb on + strandat 2964.878 kb on - strand, within escIat 2964.878 kb on - strand, within escIat 2964.878 kb on - strand, within escIat 2964.982 kb on + strand, within escIat 2965.038 kb on + strand, within escI

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Per-strain Table

Position Strand Gene LocusTag Fraction JK42
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2,962,771 - escC OKFHMN_15330 0.39 +0.0
2,962,858 - escC OKFHMN_15330 0.45 -0.5
2,963,127 + escC OKFHMN_15330 0.63 +0.8
2,963,127 + escC OKFHMN_15330 0.63 -0.6
2,963,446 + escC OKFHMN_15330 0.83 -2.5
2,963,775 + sepD OKFHMN_15335 0.16 +0.3
2,964,096 + sepD OKFHMN_15335 0.86 +0.2
2,964,139 - -1.1
2,964,158 + -1.1
2,964,272 + escJ OKFHMN_15340 0.19 +0.2
2,964,721 + +0.2
2,964,878 - escI OKFHMN_15345 0.37 +0.0
2,964,878 - escI OKFHMN_15345 0.37 -0.2
2,964,878 - escI OKFHMN_15345 0.37 -0.3
2,964,982 + escI OKFHMN_15345 0.64 +1.5
2,965,038 + escI OKFHMN_15345 0.79 -2.6

Or see this region's nucleotide sequence