Experiment: JK42
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pcnB and folK overlap by 4 nucleotides folK and OKFHMN_10365 are separated by 280 nucleotides OKFHMN_10365 and ecpD are separated by 86 nucleotides ecpD and fimD are separated by 41 nucleotides
OKFHMN_10355: pcnB - polynucleotide adenylyltransferase PcnB, at 1,939,777 to 1,941,141
pcnB
OKFHMN_10360: folK - 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase, at 1,941,138 to 1,941,620
folK
OKFHMN_10365: OKFHMN_10365 - fimbrial protein, at 1,941,901 to 1,942,497
_10365
OKFHMN_10370: ecpD - fimbrial chaperone, at 1,942,584 to 1,943,309
ecpD
OKFHMN_10375: fimD - outer membrane usher protein, at 1,943,351 to 1,945,951
fimD
Position (kb) 1941
1942
1943 Strain fitness (log2 ratio) -2
-1
0
1
2 at 1941.568 kb on + strand, within folK at 1941.569 kb on - strand, within folK at 1941.645 kb on + strand at 1941.793 kb on - strand at 1941.849 kb on + strand at 1941.899 kb on + strand at 1941.960 kb on - strand at 1941.960 kb on - strand at 1941.988 kb on - strand, within OKFHMN_10365 at 1942.023 kb on - strand, within OKFHMN_10365 at 1942.033 kb on - strand, within OKFHMN_10365 at 1942.038 kb on + strand, within OKFHMN_10365 at 1942.039 kb on - strand, within OKFHMN_10365 at 1942.039 kb on - strand, within OKFHMN_10365 at 1942.188 kb on - strand, within OKFHMN_10365 at 1942.233 kb on + strand, within OKFHMN_10365 at 1942.234 kb on - strand, within OKFHMN_10365 at 1942.239 kb on + strand, within OKFHMN_10365 at 1942.403 kb on + strand, within OKFHMN_10365 at 1942.404 kb on - strand, within OKFHMN_10365 at 1942.423 kb on - strand, within OKFHMN_10365 at 1942.460 kb on + strand at 1942.478 kb on - strand at 1942.489 kb on + strand at 1942.489 kb on + strand at 1942.490 kb on - strand at 1942.490 kb on - strand at 1942.491 kb on + strand at 1942.563 kb on + strand at 1942.583 kb on + strand at 1942.583 kb on + strand at 1942.584 kb on - strand at 1942.665 kb on - strand, within ecpD at 1942.668 kb on - strand, within ecpD at 1942.683 kb on - strand, within ecpD at 1942.692 kb on - strand, within ecpD at 1942.748 kb on - strand, within ecpD at 1942.875 kb on + strand, within ecpD at 1942.875 kb on + strand, within ecpD at 1942.876 kb on - strand, within ecpD at 1942.876 kb on - strand, within ecpD at 1942.947 kb on - strand, within ecpD at 1942.947 kb on - strand, within ecpD at 1942.981 kb on + strand, within ecpD at 1943.102 kb on + strand, within ecpD at 1943.116 kb on - strand, within ecpD at 1943.118 kb on + strand, within ecpD at 1943.119 kb on - strand, within ecpD at 1943.130 kb on - strand, within ecpD at 1943.355 kb on - strand at 1943.423 kb on + strand at 1943.423 kb on + strand at 1943.424 kb on - strand at 1943.425 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction JK42 remove 1,941,568 + folK OKFHMN_10360 0.89 +0.4 1,941,569 - folK OKFHMN_10360 0.89 +0.6 1,941,645 + +0.5 1,941,793 - +1.9 1,941,849 + +0.2 1,941,899 + -0.1 1,941,960 - -0.4 1,941,960 - -1.3 1,941,988 - OKFHMN_10365 0.15 -0.5 1,942,023 - OKFHMN_10365 0.20 +0.3 1,942,033 - OKFHMN_10365 0.22 -1.1 1,942,038 + OKFHMN_10365 0.23 -0.0 1,942,039 - OKFHMN_10365 0.23 +1.9 1,942,039 - OKFHMN_10365 0.23 -0.1 1,942,188 - OKFHMN_10365 0.48 +1.2 1,942,233 + OKFHMN_10365 0.56 -1.5 1,942,234 - OKFHMN_10365 0.56 +0.3 1,942,239 + OKFHMN_10365 0.57 -1.6 1,942,403 + OKFHMN_10365 0.84 -0.0 1,942,404 - OKFHMN_10365 0.84 -0.7 1,942,423 - OKFHMN_10365 0.87 -0.3 1,942,460 + -0.9 1,942,478 - -2.3 1,942,489 + -1.4 1,942,489 + +1.1 1,942,490 - +0.4 1,942,490 - +0.0 1,942,491 + +1.0 1,942,563 + +0.9 1,942,583 + +0.3 1,942,583 + -0.2 1,942,584 - +0.7 1,942,665 - ecpD OKFHMN_10370 0.11 +0.0 1,942,668 - ecpD OKFHMN_10370 0.12 +0.4 1,942,683 - ecpD OKFHMN_10370 0.14 +0.0 1,942,692 - ecpD OKFHMN_10370 0.15 +0.1 1,942,748 - ecpD OKFHMN_10370 0.23 -0.4 1,942,875 + ecpD OKFHMN_10370 0.40 -0.5 1,942,875 + ecpD OKFHMN_10370 0.40 +0.1 1,942,876 - ecpD OKFHMN_10370 0.40 +0.4 1,942,876 - ecpD OKFHMN_10370 0.40 +1.9 1,942,947 - ecpD OKFHMN_10370 0.50 +0.4 1,942,947 - ecpD OKFHMN_10370 0.50 -0.2 1,942,981 + ecpD OKFHMN_10370 0.55 -0.1 1,943,102 + ecpD OKFHMN_10370 0.71 +0.7 1,943,116 - ecpD OKFHMN_10370 0.73 -0.0 1,943,118 + ecpD OKFHMN_10370 0.74 +0.1 1,943,119 - ecpD OKFHMN_10370 0.74 +1.3 1,943,130 - ecpD OKFHMN_10370 0.75 +1.0 1,943,355 - -0.1 1,943,423 + +0.2 1,943,423 + -0.4 1,943,424 - -0.3 1,943,425 + +0.3
Or see this region's nucleotide sequence