Strain Fitness in Escherichia coli ECRC100 around OKFHMN_07745

Experiment: JK42

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrhsA and OKFHMN_07740 are separated by 26 nucleotidesOKFHMN_07740 and tnp-ISAs1 are separated by 214 nucleotidestnp-ISAs1 and nrfB are separated by 265 nucleotides OKFHMN_07735: rhsA - type IV secretion protein Rhs, at 1,405,768 to 1,407,102 rhsA OKFHMN_07740: OKFHMN_07740 - Immunity protein of metallopeptidase 4 toxin, at 1,407,129 to 1,407,350 _07740 OKFHMN_07745: tnp-ISAs1 - ISAs1 family transposase, at 1,407,565 to 1,408,701 tnp-ISAs1 OKFHMN_07750: nrfB - phage adsorption protein NrfB, at 1,408,967 to 1,411,204 nrfB Position (kb) 1407 1408 1409Strain fitness (log2 ratio) -1 0 1 2 3at 1406.608 kb on + strand, within rhsAat 1406.686 kb on - strand, within rhsAat 1406.891 kb on + strand, within rhsAat 1407.702 kb on + strand, within tnp-ISAs1at 1408.775 kb on + strandat 1408.775 kb on + strandat 1408.776 kb on - strandat 1408.876 kb on + strandat 1408.880 kb on + strandat 1408.919 kb on + strandat 1408.925 kb on - strandat 1409.157 kb on - strandat 1409.158 kb on + strandat 1409.264 kb on + strand, within nrfBat 1409.270 kb on - strand, within nrfBat 1409.270 kb on - strand, within nrfBat 1409.351 kb on - strand, within nrfBat 1409.556 kb on - strand, within nrfBat 1409.598 kb on - strand, within nrfB

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Per-strain Table

Position Strand Gene LocusTag Fraction JK42
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1,406,608 + rhsA OKFHMN_07735 0.63 -0.4
1,406,686 - rhsA OKFHMN_07735 0.69 +1.4
1,406,891 + rhsA OKFHMN_07735 0.84 +1.7
1,407,702 + tnp-ISAs1 OKFHMN_07745 0.12 +1.2
1,408,775 + +0.7
1,408,775 + +1.7
1,408,776 - -0.1
1,408,876 + +3.0
1,408,880 + -0.3
1,408,919 + +1.7
1,408,925 - +0.7
1,409,157 - -0.6
1,409,158 + +0.1
1,409,264 + nrfB OKFHMN_07750 0.13 -0.9
1,409,270 - nrfB OKFHMN_07750 0.14 +0.3
1,409,270 - nrfB OKFHMN_07750 0.14 -0.5
1,409,351 - nrfB OKFHMN_07750 0.17 +0.4
1,409,556 - nrfB OKFHMN_07750 0.26 +2.7
1,409,598 - nrfB OKFHMN_07750 0.28 +1.4

Or see this region's nucleotide sequence