Strain Fitness in Escherichia coli ECRC100 around OKFHMN_07000

Experiment: JK42

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntnei and pxpA are separated by 35 nucleotidespxpA and pxpC overlap by 11 nucleotidespxpC and pxpB overlap by 7 nucleotidespxpB and ybgI are separated by 22 nucleotides OKFHMN_06990: nei - endonuclease VIII, at 1,249,932 to 1,250,723 nei OKFHMN_06995: pxpA - 5-oxoprolinase subunit PxpA, at 1,250,759 to 1,251,493 pxpA OKFHMN_07000: pxpC - 5-oxoprolinase subunit PxpC, at 1,251,483 to 1,252,415 pxpC OKFHMN_07005: pxpB - 5-oxoprolinase subunit PxpB, at 1,252,409 to 1,253,065 pxpB OKFHMN_07010: ybgI - radiation resistance protein YbgI, at 1,253,088 to 1,253,831 ybgI Position (kb) 1251 1252 1253Strain fitness (log2 ratio) -2 -1 0 1 2at 1250.528 kb on - strand, within neiat 1250.536 kb on - strand, within neiat 1250.556 kb on + strand, within neiat 1250.556 kb on + strand, within neiat 1250.557 kb on - strand, within neiat 1250.589 kb on - strand, within neiat 1250.617 kb on + strand, within neiat 1250.747 kb on + strandat 1250.761 kb on - strandat 1250.802 kb on - strandat 1251.112 kb on - strand, within pxpAat 1251.114 kb on - strand, within pxpAat 1251.165 kb on + strand, within pxpAat 1251.166 kb on - strand, within pxpAat 1251.198 kb on - strand, within pxpAat 1251.243 kb on + strand, within pxpAat 1251.259 kb on - strand, within pxpAat 1251.438 kb on - strandat 1251.438 kb on - strandat 1251.510 kb on + strandat 1251.620 kb on + strand, within pxpCat 1251.620 kb on + strand, within pxpCat 1251.621 kb on - strand, within pxpCat 1251.636 kb on - strand, within pxpCat 1251.645 kb on - strand, within pxpCat 1251.816 kb on - strand, within pxpCat 1251.872 kb on - strand, within pxpCat 1252.167 kb on - strand, within pxpCat 1252.167 kb on - strand, within pxpCat 1252.167 kb on - strand, within pxpCat 1252.316 kb on + strand, within pxpCat 1252.386 kb on - strandat 1252.388 kb on - strandat 1252.388 kb on - strandat 1252.415 kb on + strandat 1252.460 kb on + strandat 1252.464 kb on - strandat 1252.508 kb on - strand, within pxpBat 1252.629 kb on - strand, within pxpBat 1252.909 kb on + strand, within pxpBat 1252.910 kb on - strand, within pxpBat 1253.047 kb on - strandat 1253.089 kb on + strandat 1253.090 kb on - strandat 1253.090 kb on - strandat 1253.166 kb on - strand, within ybgIat 1253.201 kb on - strand, within ybgIat 1253.375 kb on + strand, within ybgI

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Per-strain Table

Position Strand Gene LocusTag Fraction JK42
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1,250,528 - nei OKFHMN_06990 0.75 +0.8
1,250,536 - nei OKFHMN_06990 0.76 -1.2
1,250,556 + nei OKFHMN_06990 0.79 -1.1
1,250,556 + nei OKFHMN_06990 0.79 -1.2
1,250,557 - nei OKFHMN_06990 0.79 -0.5
1,250,589 - nei OKFHMN_06990 0.83 +0.4
1,250,617 + nei OKFHMN_06990 0.86 +0.5
1,250,747 + +0.6
1,250,761 - -0.1
1,250,802 - -0.4
1,251,112 - pxpA OKFHMN_06995 0.48 -0.1
1,251,114 - pxpA OKFHMN_06995 0.48 -0.5
1,251,165 + pxpA OKFHMN_06995 0.55 -0.9
1,251,166 - pxpA OKFHMN_06995 0.55 -0.5
1,251,198 - pxpA OKFHMN_06995 0.60 -0.2
1,251,243 + pxpA OKFHMN_06995 0.66 +0.4
1,251,259 - pxpA OKFHMN_06995 0.68 +0.8
1,251,438 - +0.6
1,251,438 - -1.6
1,251,510 + -0.4
1,251,620 + pxpC OKFHMN_07000 0.15 -1.7
1,251,620 + pxpC OKFHMN_07000 0.15 +0.2
1,251,621 - pxpC OKFHMN_07000 0.15 -0.0
1,251,636 - pxpC OKFHMN_07000 0.16 +1.6
1,251,645 - pxpC OKFHMN_07000 0.17 -1.3
1,251,816 - pxpC OKFHMN_07000 0.36 +0.8
1,251,872 - pxpC OKFHMN_07000 0.42 -0.6
1,252,167 - pxpC OKFHMN_07000 0.73 -0.6
1,252,167 - pxpC OKFHMN_07000 0.73 +2.2
1,252,167 - pxpC OKFHMN_07000 0.73 +1.1
1,252,316 + pxpC OKFHMN_07000 0.89 -1.4
1,252,386 - -0.2
1,252,388 - -0.9
1,252,388 - -0.6
1,252,415 + +1.1
1,252,460 + -0.3
1,252,464 - -0.2
1,252,508 - pxpB OKFHMN_07005 0.15 +0.1
1,252,629 - pxpB OKFHMN_07005 0.33 +0.2
1,252,909 + pxpB OKFHMN_07005 0.76 -0.5
1,252,910 - pxpB OKFHMN_07005 0.76 -0.4
1,253,047 - -0.6
1,253,089 + +0.9
1,253,090 - +0.6
1,253,090 - +0.5
1,253,166 - ybgI OKFHMN_07010 0.10 -1.8
1,253,201 - ybgI OKFHMN_07010 0.15 -0.3
1,253,375 + ybgI OKFHMN_07010 0.39 -0.9

Or see this region's nucleotide sequence