Strain Fitness in Escherichia coli ECRC100 around OKFHMN_06360

Experiment: JK42

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntybhL and moaE are separated by 135 nucleotidesmoaE and moaD are separated by 1 nucleotidesmoaD and moaC overlap by 8 nucleotidesmoaC and moaB are separated by 2 nucleotidesmoaB and moaA are separated by 21 nucleotides OKFHMN_06345: ybhL - Inner membrane protein YbhL, at 1,130,322 to 1,131,026 ybhL OKFHMN_06350: moaE - molybdopterin synthase catalytic subunit MoaE, at 1,131,162 to 1,131,614 moaE OKFHMN_06355: moaD - molybdopterin synthase sulfur carrier subunit, at 1,131,616 to 1,131,861 moaD OKFHMN_06360: moaC - cyclic pyranopterin monophosphate synthase MoaC, at 1,131,854 to 1,132,339 moaC OKFHMN_06365: moaB - molybdenum cofactor biosynthesis protein B, at 1,132,342 to 1,132,854 moaB OKFHMN_06370: moaA - GTP 3',8-cyclase MoaA, at 1,132,876 to 1,133,865 moaA Position (kb) 1131 1132 1133Strain fitness (log2 ratio) -1 0 1 2at 1130.954 kb on - strand, within ybhLat 1130.966 kb on + strandat 1130.968 kb on + strandat 1130.968 kb on + strandat 1131.044 kb on - strandat 1131.052 kb on + strandat 1131.057 kb on + strandat 1131.057 kb on + strandat 1131.163 kb on + strandat 1131.352 kb on - strand, within moaEat 1131.618 kb on - strandat 1131.709 kb on + strand, within moaDat 1131.960 kb on + strand, within moaCat 1131.960 kb on + strand, within moaCat 1132.044 kb on - strand, within moaCat 1132.162 kb on + strand, within moaCat 1132.163 kb on - strand, within moaCat 1132.179 kb on - strand, within moaCat 1132.339 kb on + strandat 1132.344 kb on - strandat 1132.344 kb on - strandat 1132.344 kb on - strandat 1132.369 kb on - strandat 1132.533 kb on + strand, within moaBat 1132.533 kb on + strand, within moaBat 1132.672 kb on + strand, within moaBat 1132.798 kb on - strand, within moaBat 1132.844 kb on - strandat 1132.908 kb on + strandat 1133.142 kb on - strand, within moaA

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Per-strain Table

Position Strand Gene LocusTag Fraction JK42
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1,130,954 - ybhL OKFHMN_06345 0.90 +0.6
1,130,966 + -0.5
1,130,968 + -0.2
1,130,968 + +2.2
1,131,044 - +0.4
1,131,052 + +0.9
1,131,057 + -0.4
1,131,057 + +0.3
1,131,163 + +0.6
1,131,352 - moaE OKFHMN_06350 0.42 +0.2
1,131,618 - +0.1
1,131,709 + moaD OKFHMN_06355 0.38 +0.3
1,131,960 + moaC OKFHMN_06360 0.22 +0.0
1,131,960 + moaC OKFHMN_06360 0.22 +0.6
1,132,044 - moaC OKFHMN_06360 0.39 -0.7
1,132,162 + moaC OKFHMN_06360 0.63 +0.6
1,132,163 - moaC OKFHMN_06360 0.64 -0.4
1,132,179 - moaC OKFHMN_06360 0.67 +0.7
1,132,339 + +2.6
1,132,344 - +0.2
1,132,344 - +0.8
1,132,344 - +0.1
1,132,369 - -0.0
1,132,533 + moaB OKFHMN_06365 0.37 +0.3
1,132,533 + moaB OKFHMN_06365 0.37 -0.0
1,132,672 + moaB OKFHMN_06365 0.64 +0.1
1,132,798 - moaB OKFHMN_06365 0.89 -0.0
1,132,844 - +0.2
1,132,908 + -0.5
1,133,142 - moaA OKFHMN_06370 0.27 -0.7

Or see this region's nucleotide sequence