Experiment: JK42
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rlmC and ybjO are separated by 40 nucleotides ybjO and potI are separated by 59 nucleotides potI and potH overlap by 4 nucleotides potH and potG are separated by 9 nucleotides
OKFHMN_05935: rlmC - 23S rRNA (uracil(747)-C(5))-methyltransferase RlmC, at 1,048,342 to 1,049,469
rlmC
OKFHMN_05940: ybjO - Inner membrane protein YbjO, at 1,049,510 to 1,049,998
ybjO
OKFHMN_05945: potI - putrescine ABC transporter permease PotI, at 1,050,058 to 1,050,903
potI
OKFHMN_05950: potH - putrescine ABC transporter permease PotH, at 1,050,900 to 1,051,853
potH
OKFHMN_05955: potG - putrescine ABC transporter ATP-binding subunit PotG, at 1,051,863 to 1,052,996
potG
Position (kb)
1050
1051 Strain fitness (log2 ratio)
-1
0
1
2 at 1049.132 kb on + strand, within rlmC at 1049.275 kb on - strand, within rlmC at 1049.299 kb on - strand, within rlmC at 1049.373 kb on + strand at 1049.470 kb on - strand at 1049.504 kb on + strand at 1049.504 kb on + strand at 1049.511 kb on + strand at 1049.899 kb on + strand, within ybjO at 1049.899 kb on + strand, within ybjO at 1050.018 kb on - strand at 1050.027 kb on + strand at 1050.028 kb on - strand at 1050.028 kb on - strand at 1050.028 kb on - strand at 1050.028 kb on - strand at 1050.032 kb on - strand at 1050.120 kb on - strand at 1050.170 kb on - strand, within potI at 1050.289 kb on - strand, within potI at 1050.394 kb on + strand, within potI at 1050.429 kb on + strand, within potI at 1050.443 kb on + strand, within potI at 1050.480 kb on + strand, within potI at 1050.651 kb on + strand, within potI at 1050.651 kb on + strand, within potI at 1051.142 kb on + strand, within potH at 1051.291 kb on + strand, within potH at 1051.291 kb on + strand, within potH at 1051.292 kb on - strand, within potH at 1051.399 kb on + strand, within potH at 1051.444 kb on - strand, within potH at 1051.447 kb on - strand, within potH at 1051.447 kb on - strand, within potH at 1051.454 kb on + strand, within potH at 1051.472 kb on + strand, within potH at 1051.657 kb on + strand, within potH at 1051.658 kb on - strand, within potH at 1051.693 kb on - strand, within potH at 1051.742 kb on + strand, within potH at 1051.805 kb on + strand at 1051.847 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction JK42 remove 1,049,132 + rlmC OKFHMN_05935 0.70 +0.7 1,049,275 - rlmC OKFHMN_05935 0.83 +1.1 1,049,299 - rlmC OKFHMN_05935 0.85 -1.4 1,049,373 + -0.7 1,049,470 - -1.1 1,049,504 + -1.2 1,049,504 + -1.4 1,049,511 + -0.3 1,049,899 + ybjO OKFHMN_05940 0.80 +0.6 1,049,899 + ybjO OKFHMN_05940 0.80 +0.3 1,050,018 - +1.6 1,050,027 + -0.2 1,050,028 - +0.7 1,050,028 - -0.5 1,050,028 - -1.0 1,050,028 - -0.0 1,050,032 - +1.6 1,050,120 - +1.1 1,050,170 - potI OKFHMN_05945 0.13 -0.5 1,050,289 - potI OKFHMN_05945 0.27 -0.6 1,050,394 + potI OKFHMN_05945 0.40 -0.4 1,050,429 + potI OKFHMN_05945 0.44 -0.0 1,050,443 + potI OKFHMN_05945 0.46 -1.3 1,050,480 + potI OKFHMN_05945 0.50 -0.2 1,050,651 + potI OKFHMN_05945 0.70 +0.1 1,050,651 + potI OKFHMN_05945 0.70 +0.6 1,051,142 + potH OKFHMN_05950 0.25 +0.3 1,051,291 + potH OKFHMN_05950 0.41 -0.4 1,051,291 + potH OKFHMN_05950 0.41 +1.6 1,051,292 - potH OKFHMN_05950 0.41 -0.5 1,051,399 + potH OKFHMN_05950 0.52 +2.2 1,051,444 - potH OKFHMN_05950 0.57 +0.7 1,051,447 - potH OKFHMN_05950 0.57 +1.4 1,051,447 - potH OKFHMN_05950 0.57 -0.1 1,051,454 + potH OKFHMN_05950 0.58 -0.7 1,051,472 + potH OKFHMN_05950 0.60 -0.7 1,051,657 + potH OKFHMN_05950 0.79 -0.4 1,051,658 - potH OKFHMN_05950 0.79 -0.2 1,051,693 - potH OKFHMN_05950 0.83 +0.6 1,051,742 + potH OKFHMN_05950 0.88 +1.6 1,051,805 + +1.2 1,051,847 + -0.0
Or see this region's nucleotide sequence