Strain Fitness in Escherichia coli ECRC100 around OKFHMN_04530

Experiment: JK42

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntcadC1 and OKFHMN_04525 are separated by 4 nucleotidesOKFHMN_04525 and eAL are separated by 101 nucleotideseAL and dgcT are separated by 97 nucleotides OKFHMN_04520: cadC1 - transcriptional regulator, at 738,431 to 739,243 cadC1 OKFHMN_04525: OKFHMN_04525 - FidL, at 739,248 to 739,739 _04525 OKFHMN_04530: eAL - EAL domain, c-di-GMP-specific phosphodiesterase class I (or its enzymatically inactive variant), at 739,841 to 741,451 eAL OKFHMN_04535: dgcT - putative diguanylate cyclase DgcT, at 741,549 to 742,907 dgcT Position (kb) 739 740 741 742Strain fitness (log2 ratio) -1 0 1at 741.693 kb on - strand, within dgcTat 741.722 kb on - strand, within dgcTat 741.862 kb on - strand, within dgcTat 742.320 kb on - strand, within dgcT

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Per-strain Table

Position Strand Gene LocusTag Fraction JK42
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741,693 - dgcT OKFHMN_04535 0.11 +0.3
741,722 - dgcT OKFHMN_04535 0.13 -0.2
741,862 - dgcT OKFHMN_04535 0.23 -0.8
742,320 - dgcT OKFHMN_04535 0.57 -0.6

Or see this region's nucleotide sequence