Strain Fitness in Escherichia coli ECRC100 around OKFHMN_03290

Experiment: JK42

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntOKFHMN_03270 and OKFHMN_03275 are separated by 4 nucleotidesOKFHMN_03275 and OKFHMN_03280 are separated by 75 nucleotidesOKFHMN_03280 and OKFHMN_03285 are separated by 37 nucleotidesOKFHMN_03285 and OKFHMN_03290 are separated by 147 nucleotidesOKFHMN_03290 and OKFHMN_03300 are separated by 314 nucleotidesOKFHMN_03300 and OKFHMN_03305 overlap by 4 nucleotides OKFHMN_03270: OKFHMN_03270 - DUF1327 domain-containing protein, at 517,792 to 518,136 _03270 OKFHMN_03275: OKFHMN_03275 - holin, at 518,141 to 518,356 _03275 OKFHMN_03280: OKFHMN_03280 - DUF826 domain-containing protein, at 518,432 to 518,701 _03280 OKFHMN_03285: OKFHMN_03285 - DUF1378 domain-containing protein, at 518,739 to 518,921 _03285 OKFHMN_03290: OKFHMN_03290 - sialate O-acetylesterase, at 519,069 to 521,006 _03290 OKFHMN_03300: OKFHMN_03300 - TciB/TerA-like protein, at 521,321 to 521,488 _03300 OKFHMN_03305: OKFHMN_03305 - TciA/TerB-like protein, at 521,485 to 521,631 _03305 Position (kb) 519 520 521 522Strain fitness (log2 ratio) -1 0 1 2at 518.903 kb on + strandat 518.904 kb on - strandat 518.941 kb on + strandat 519.094 kb on - strandat 519.470 kb on + strand, within OKFHMN_03290at 520.345 kb on + strand, within OKFHMN_03290at 520.346 kb on - strand, within OKFHMN_03290at 520.346 kb on - strand, within OKFHMN_03290at 520.346 kb on - strand, within OKFHMN_03290at 520.374 kb on - strand, within OKFHMN_03290at 520.405 kb on + strand, within OKFHMN_03290at 520.405 kb on + strand, within OKFHMN_03290at 520.406 kb on - strand, within OKFHMN_03290at 520.406 kb on - strand, within OKFHMN_03290at 520.683 kb on - strand, within OKFHMN_03290at 520.732 kb on + strand, within OKFHMN_03290at 520.771 kb on + strand, within OKFHMN_03290at 520.777 kb on + strand, within OKFHMN_03290at 520.778 kb on - strand, within OKFHMN_03290at 521.896 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction JK42
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518,903 + +1.6
518,904 - -0.0
518,941 + +0.6
519,094 - -0.7
519,470 + OKFHMN_03290 0.21 +2.0
520,345 + OKFHMN_03290 0.66 -0.5
520,346 - OKFHMN_03290 0.66 -0.0
520,346 - OKFHMN_03290 0.66 +1.6
520,346 - OKFHMN_03290 0.66 +0.4
520,374 - OKFHMN_03290 0.67 -0.8
520,405 + OKFHMN_03290 0.69 +0.4
520,405 + OKFHMN_03290 0.69 -0.8
520,406 - OKFHMN_03290 0.69 -0.0
520,406 - OKFHMN_03290 0.69 -0.8
520,683 - OKFHMN_03290 0.83 -0.9
520,732 + OKFHMN_03290 0.86 +2.6
520,771 + OKFHMN_03290 0.88 +0.8
520,777 + OKFHMN_03290 0.88 -1.5
520,778 - OKFHMN_03290 0.88 -1.3
521,896 - -0.0

Or see this region's nucleotide sequence