Strain Fitness in Escherichia coli ECRC100 around OKFHMN_02750

Experiment: JK42

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntnleH1-2 and OKFHMN_02750 are separated by 473 nucleotidesOKFHMN_02750 and nleA are separated by 660 nucleotides OKFHMN_02745: nleH1-2 - type III secretion system effector kinase NleH1-2, at 439,310 to 440,221 nleH1-2 OKFHMN_02750: OKFHMN_02750 - Tyr recombinase domain-containing protein, at 440,695 to 441,387 _02750 OKFHMN_02755: nleA - type III secretion system effector NleA, at 442,048 to 443,373 nleA Position (kb) 440 441 442Strain fitness (log2 ratio) -1 0 1at 440.070 kb on + strand, within nleH1-2at 440.139 kb on - strandat 440.201 kb on + strandat 440.247 kb on - strandat 440.250 kb on + strandat 440.547 kb on + strandat 440.869 kb on + strand, within OKFHMN_02750at 440.931 kb on - strand, within OKFHMN_02750at 440.931 kb on - strand, within OKFHMN_02750at 441.019 kb on - strand, within OKFHMN_02750at 441.171 kb on - strand, within OKFHMN_02750at 441.250 kb on - strand, within OKFHMN_02750at 441.713 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction JK42
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440,070 + nleH1-2 OKFHMN_02745 0.83 +0.8
440,139 - -1.3
440,201 + -0.5
440,247 - +1.5
440,250 + -0.3
440,547 + -0.8
440,869 + OKFHMN_02750 0.25 -0.0
440,931 - OKFHMN_02750 0.34 +0.8
440,931 - OKFHMN_02750 0.34 +0.3
441,019 - OKFHMN_02750 0.47 -0.5
441,171 - OKFHMN_02750 0.69 +0.8
441,250 - OKFHMN_02750 0.80 +0.5
441,713 + +0.8

Or see this region's nucleotide sequence