Strain Fitness in Escherichia coli ECRC100 around OKFHMN_02710

Experiment: JK42

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntnleG and OKFHMN_02710 are separated by 754 nucleotidesOKFHMN_02710 and tnpB are separated by 304 nucleotidestnpB and tnpB overlap by 4 nucleotides OKFHMN_02705: nleG - T3SS effector E3 ubiquitin-protein ligase NleG, at 433,422 to 434,069 nleG OKFHMN_02710: OKFHMN_02710 - hypothetical protein, at 434,824 to 435,093 _02710 OKFHMN_02715: tnpB - IS66 family insertion sequence element accessory protein TnpB, at 435,398 to 435,778 tnpB OKFHMN_02720: tnpB - IS66 family insertion sequence element accessory protein TnpB, at 435,775 to 436,122 tnpB Position (kb) 434 435 436Strain fitness (log2 ratio) -1 0 1at 433.900 kb on + strand, within nleGat 435.061 kb on - strand, within OKFHMN_02710at 435.308 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction JK42
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433,900 + nleG OKFHMN_02705 0.74 -1.5
435,061 - OKFHMN_02710 0.88 +0.7
435,308 - -0.6

Or see this region's nucleotide sequence