Strain Fitness in Escherichia coli ECRC100 around OKFHMN_02655

Experiment: JK42

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyciV and trpL are separated by 137 nucleotidestrpL and trpE are separated by 91 nucleotidestrpE and trpD overlap by 1 nucleotides OKFHMN_02645: yciV - 5'-3' exoribonuclease, at 419,794 to 420,675 yciV OKFHMN_02650: trpL - trp operon leader peptide, at 420,813 to 420,857 trpL OKFHMN_02655: trpE - anthranilate synthase component I, at 420,949 to 422,511 trpE OKFHMN_02660: trpD - bifunctional anthranilate synthase glutamate amidotransferase component TrpG/anthranilate phosphoribosyltransferase TrpD, at 422,511 to 424,106 trpD Position (kb) 420 421 422 423Strain fitness (log2 ratio) -1 0 1 2at 419.968 kb on - strand, within yciVat 420.089 kb on - strand, within yciVat 420.141 kb on + strand, within yciVat 420.308 kb on - strand, within yciVat 420.308 kb on - strand, within yciVat 420.427 kb on - strand, within yciVat 420.829 kb on + strand, within trpLat 420.882 kb on + strandat 421.792 kb on + strand, within trpEat 421.809 kb on + strand, within trpEat 421.822 kb on + strand, within trpEat 421.823 kb on - strand, within trpEat 422.126 kb on - strand, within trpEat 422.126 kb on - strand, within trpEat 422.132 kb on - strand, within trpEat 422.258 kb on + strand, within trpEat 422.259 kb on - strand, within trpEat 422.589 kb on - strandat 422.708 kb on + strand, within trpDat 423.492 kb on - strand, within trpD

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Per-strain Table

Position Strand Gene LocusTag Fraction JK42
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419,968 - yciV OKFHMN_02645 0.20 +0.2
420,089 - yciV OKFHMN_02645 0.33 -0.1
420,141 + yciV OKFHMN_02645 0.39 +2.5
420,308 - yciV OKFHMN_02645 0.58 +0.9
420,308 - yciV OKFHMN_02645 0.58 +0.5
420,427 - yciV OKFHMN_02645 0.72 -0.4
420,829 + trpL OKFHMN_02650 0.36 +0.4
420,882 + +0.2
421,792 + trpE OKFHMN_02655 0.54 +0.5
421,809 + trpE OKFHMN_02655 0.55 +0.5
421,822 + trpE OKFHMN_02655 0.56 -0.5
421,823 - trpE OKFHMN_02655 0.56 -0.8
422,126 - trpE OKFHMN_02655 0.75 +1.5
422,126 - trpE OKFHMN_02655 0.75 +0.7
422,132 - trpE OKFHMN_02655 0.76 -0.7
422,258 + trpE OKFHMN_02655 0.84 -0.9
422,259 - trpE OKFHMN_02655 0.84 +0.2
422,589 - -0.0
422,708 + trpD OKFHMN_02660 0.12 -1.6
423,492 - trpD OKFHMN_02660 0.61 +0.5

Or see this region's nucleotide sequence