Experiment: JK42
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dppD and ddpF overlap by 8 nucleotides ddpF and osmC are separated by 55 nucleotides osmC and bdm are separated by 344 nucleotides bdm and sra are separated by 101 nucleotides sra and maeA are separated by 156 nucleotides
OKFHMN_01335: dppD - ABC transporter ATP-binding protein, at 170,797 to 171,783
dppD
OKFHMN_01340: ddpF - putative D,D-dipeptide transport ATP-binding protein DdpF, at 171,776 to 172,702
ddpF
OKFHMN_01345: osmC - peroxiredoxin OsmC, at 172,758 to 173,189
osmC
OKFHMN_01350: bdm - biofilm-dependent modulation protein, at 173,534 to 173,749
bdm
OKFHMN_01355: sra - stationary-phase-induced ribosome-associated protein, at 173,851 to 173,988
sra
OKFHMN_01360: maeA - malate dehydrogenase, at 174,145 to 175,842
maeA
Position (kb) 172
173
174 Strain fitness (log2 ratio) -1
0
1 at 171.872 kb on - strand, within ddpF at 172.105 kb on + strand, within ddpF at 173.125 kb on - strand, within osmC at 173.384 kb on + strand at 173.479 kb on + strand at 173.773 kb on + strand at 173.922 kb on + strand, within sra at 173.923 kb on - strand, within sra at 174.063 kb on - strand at 174.063 kb on - strand at 174.064 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction JK42 remove 171,872 - ddpF OKFHMN_01340 0.10 +1.4 172,105 + ddpF OKFHMN_01340 0.35 -1.5 173,125 - osmC OKFHMN_01345 0.85 +1.1 173,384 + +0.4 173,479 + -0.6 173,773 + +0.2 173,922 + sra OKFHMN_01355 0.51 -1.4 173,923 - sra OKFHMN_01355 0.52 -0.1 174,063 - +0.8 174,063 - +0.6 174,064 + +0.1
Or see this region's nucleotide sequence