Strain Fitness in Escherichia coli ECRC100 around OKFHMN_00270

Experiment: JK42

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntgspJ and gspI overlap by 4 nucleotidesgspI and gspH overlap by 4 nucleotidesgspH and gspG overlap by 4 nucleotidesgspG and gspF are separated by 30 nucleotides OKFHMN_00255: gspJ - type II secretion system minor pseudopilin GspJ, at 53,040 to 53,639 gspJ OKFHMN_00260: gspI - type II secretion system minor pseudopilin GspI, at 53,636 to 54,001 gspI OKFHMN_00265: gspH - type II secretion system minor pseudopilin GspH, at 53,998 to 54,552 gspH OKFHMN_00270: gspG - type II secretion system major pseudopilin GspG, at 54,549 to 54,983 gspG OKFHMN_00275: gspF - type II secretion system inner membrane protein GspF, at 55,014 to 56,237 gspF Position (kb) 54 55Strain fitness (log2 ratio) -2 -1 0 1 2at 53.612 kb on + strandat 53.613 kb on - strandat 53.613 kb on - strandat 53.622 kb on + strandat 53.623 kb on - strandat 53.656 kb on + strandat 53.689 kb on + strand, within gspIat 53.699 kb on + strand, within gspIat 53.699 kb on + strand, within gspIat 53.699 kb on + strand, within gspIat 53.705 kb on + strand, within gspIat 53.706 kb on - strand, within gspIat 53.706 kb on - strand, within gspIat 53.756 kb on + strand, within gspIat 53.756 kb on + strand, within gspIat 53.757 kb on - strand, within gspIat 53.757 kb on - strand, within gspIat 53.960 kb on + strand, within gspIat 53.960 kb on + strand, within gspIat 53.961 kb on - strand, within gspIat 53.961 kb on - strand, within gspIat 53.961 kb on - strand, within gspIat 53.963 kb on + strand, within gspIat 53.964 kb on - strand, within gspIat 53.964 kb on - strand, within gspIat 53.964 kb on - strand, within gspIat 54.185 kb on + strand, within gspHat 54.263 kb on + strand, within gspHat 54.263 kb on + strand, within gspHat 54.264 kb on - strand, within gspHat 54.264 kb on - strand, within gspHat 54.264 kb on - strand, within gspHat 54.277 kb on - strand, within gspHat 54.293 kb on - strand, within gspHat 54.293 kb on - strand, within gspHat 54.333 kb on + strand, within gspHat 54.393 kb on + strand, within gspHat 54.393 kb on + strand, within gspHat 54.393 kb on + strand, within gspHat 54.394 kb on - strand, within gspHat 54.404 kb on + strand, within gspHat 54.405 kb on - strand, within gspHat 54.475 kb on + strand, within gspHat 54.475 kb on + strand, within gspHat 54.475 kb on + strand, within gspHat 54.475 kb on + strand, within gspHat 54.475 kb on + strand, within gspHat 54.475 kb on + strand, within gspHat 54.476 kb on - strand, within gspHat 54.476 kb on - strand, within gspHat 54.476 kb on - strand, within gspHat 54.479 kb on - strand, within gspHat 54.585 kb on - strandat 54.629 kb on + strand, within gspGat 54.644 kb on - strand, within gspGat 54.644 kb on - strand, within gspGat 54.658 kb on - strand, within gspGat 54.667 kb on + strand, within gspGat 54.668 kb on - strand, within gspGat 54.704 kb on + strand, within gspGat 54.704 kb on + strand, within gspGat 54.721 kb on + strand, within gspGat 54.801 kb on - strand, within gspGat 54.811 kb on + strand, within gspGat 54.811 kb on + strand, within gspGat 54.812 kb on - strand, within gspGat 54.878 kb on + strand, within gspGat 54.878 kb on + strand, within gspGat 54.879 kb on - strand, within gspGat 54.933 kb on + strand, within gspGat 54.933 kb on + strand, within gspGat 54.943 kb on - strandat 54.956 kb on + strandat 54.956 kb on + strandat 54.957 kb on - strandat 54.957 kb on - strandat 55.003 kb on + strandat 55.004 kb on - strandat 55.019 kb on + strandat 55.020 kb on - strandat 55.020 kb on - strandat 55.071 kb on - strandat 55.235 kb on + strand, within gspFat 55.307 kb on + strand, within gspFat 55.311 kb on + strand, within gspFat 55.391 kb on + strand, within gspFat 55.392 kb on - strand, within gspFat 55.414 kb on + strand, within gspFat 55.414 kb on + strand, within gspFat 55.415 kb on - strand, within gspFat 55.415 kb on - strand, within gspFat 55.506 kb on + strand, within gspFat 55.506 kb on + strand, within gspFat 55.506 kb on + strand, within gspFat 55.507 kb on - strand, within gspFat 55.509 kb on + strand, within gspFat 55.509 kb on + strand, within gspFat 55.548 kb on + strand, within gspFat 55.549 kb on - strand, within gspFat 55.604 kb on + strand, within gspFat 55.604 kb on + strand, within gspFat 55.608 kb on + strand, within gspFat 55.629 kb on - strand, within gspFat 55.667 kb on + strand, within gspFat 55.668 kb on - strand, within gspFat 55.668 kb on - strand, within gspFat 55.717 kb on + strand, within gspFat 55.768 kb on + strand, within gspFat 55.768 kb on + strand, within gspFat 55.768 kb on + strand, within gspFat 55.923 kb on + strand, within gspF

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Per-strain Table

Position Strand Gene LocusTag Fraction JK42
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53,612 + +0.1
53,613 - +0.0
53,613 - +0.9
53,622 + -0.1
53,623 - +0.1
53,656 + +0.2
53,689 + gspI OKFHMN_00260 0.14 -0.3
53,699 + gspI OKFHMN_00260 0.17 -0.4
53,699 + gspI OKFHMN_00260 0.17 -0.3
53,699 + gspI OKFHMN_00260 0.17 +0.3
53,705 + gspI OKFHMN_00260 0.19 +0.3
53,706 - gspI OKFHMN_00260 0.19 -0.2
53,706 - gspI OKFHMN_00260 0.19 -0.2
53,756 + gspI OKFHMN_00260 0.33 -0.4
53,756 + gspI OKFHMN_00260 0.33 +1.7
53,757 - gspI OKFHMN_00260 0.33 +0.2
53,757 - gspI OKFHMN_00260 0.33 +0.2
53,960 + gspI OKFHMN_00260 0.89 +1.1
53,960 + gspI OKFHMN_00260 0.89 +0.1
53,961 - gspI OKFHMN_00260 0.89 -0.2
53,961 - gspI OKFHMN_00260 0.89 -0.0
53,961 - gspI OKFHMN_00260 0.89 -0.4
53,963 + gspI OKFHMN_00260 0.89 +0.2
53,964 - gspI OKFHMN_00260 0.90 -1.8
53,964 - gspI OKFHMN_00260 0.90 +1.2
53,964 - gspI OKFHMN_00260 0.90 +1.0
54,185 + gspH OKFHMN_00265 0.34 +0.2
54,263 + gspH OKFHMN_00265 0.48 -0.2
54,263 + gspH OKFHMN_00265 0.48 -0.5
54,264 - gspH OKFHMN_00265 0.48 +1.2
54,264 - gspH OKFHMN_00265 0.48 +0.9
54,264 - gspH OKFHMN_00265 0.48 +1.9
54,277 - gspH OKFHMN_00265 0.50 -0.4
54,293 - gspH OKFHMN_00265 0.53 +0.5
54,293 - gspH OKFHMN_00265 0.53 +0.5
54,333 + gspH OKFHMN_00265 0.60 +0.3
54,393 + gspH OKFHMN_00265 0.71 -0.4
54,393 + gspH OKFHMN_00265 0.71 -0.3
54,393 + gspH OKFHMN_00265 0.71 -1.6
54,394 - gspH OKFHMN_00265 0.71 +0.3
54,404 + gspH OKFHMN_00265 0.73 -0.9
54,405 - gspH OKFHMN_00265 0.73 -0.6
54,475 + gspH OKFHMN_00265 0.86 -0.8
54,475 + gspH OKFHMN_00265 0.86 +0.5
54,475 + gspH OKFHMN_00265 0.86 +0.2
54,475 + gspH OKFHMN_00265 0.86 +0.5
54,475 + gspH OKFHMN_00265 0.86 +0.7
54,475 + gspH OKFHMN_00265 0.86 -0.6
54,476 - gspH OKFHMN_00265 0.86 -0.1
54,476 - gspH OKFHMN_00265 0.86 +0.2
54,476 - gspH OKFHMN_00265 0.86 -0.3
54,479 - gspH OKFHMN_00265 0.87 -1.0
54,585 - +0.7
54,629 + gspG OKFHMN_00270 0.18 -0.1
54,644 - gspG OKFHMN_00270 0.22 -0.2
54,644 - gspG OKFHMN_00270 0.22 -0.0
54,658 - gspG OKFHMN_00270 0.25 -0.9
54,667 + gspG OKFHMN_00270 0.27 -0.7
54,668 - gspG OKFHMN_00270 0.27 +0.6
54,704 + gspG OKFHMN_00270 0.36 -1.1
54,704 + gspG OKFHMN_00270 0.36 -0.4
54,721 + gspG OKFHMN_00270 0.40 +1.6
54,801 - gspG OKFHMN_00270 0.58 -0.1
54,811 + gspG OKFHMN_00270 0.60 -0.3
54,811 + gspG OKFHMN_00270 0.60 -0.2
54,812 - gspG OKFHMN_00270 0.60 +0.8
54,878 + gspG OKFHMN_00270 0.76 +1.3
54,878 + gspG OKFHMN_00270 0.76 +0.0
54,879 - gspG OKFHMN_00270 0.76 +1.6
54,933 + gspG OKFHMN_00270 0.88 +0.2
54,933 + gspG OKFHMN_00270 0.88 -0.2
54,943 - +0.1
54,956 + -1.1
54,956 + +1.1
54,957 - +1.1
54,957 - +0.3
55,003 + -0.1
55,004 - +0.8
55,019 + +0.4
55,020 - -1.4
55,020 - -1.1
55,071 - +0.1
55,235 + gspF OKFHMN_00275 0.18 -0.5
55,307 + gspF OKFHMN_00275 0.24 +0.5
55,311 + gspF OKFHMN_00275 0.24 +0.5
55,391 + gspF OKFHMN_00275 0.31 -0.6
55,392 - gspF OKFHMN_00275 0.31 +0.1
55,414 + gspF OKFHMN_00275 0.33 -0.8
55,414 + gspF OKFHMN_00275 0.33 +0.6
55,415 - gspF OKFHMN_00275 0.33 +0.0
55,415 - gspF OKFHMN_00275 0.33 -0.8
55,506 + gspF OKFHMN_00275 0.40 +1.0
55,506 + gspF OKFHMN_00275 0.40 +1.0
55,506 + gspF OKFHMN_00275 0.40 +1.6
55,507 - gspF OKFHMN_00275 0.40 -0.3
55,509 + gspF OKFHMN_00275 0.40 -2.1
55,509 + gspF OKFHMN_00275 0.40 +0.3
55,548 + gspF OKFHMN_00275 0.44 -0.0
55,549 - gspF OKFHMN_00275 0.44 +0.2
55,604 + gspF OKFHMN_00275 0.48 +0.5
55,604 + gspF OKFHMN_00275 0.48 -0.4
55,608 + gspF OKFHMN_00275 0.49 -1.1
55,629 - gspF OKFHMN_00275 0.50 +0.3
55,667 + gspF OKFHMN_00275 0.53 +0.1
55,668 - gspF OKFHMN_00275 0.53 +0.4
55,668 - gspF OKFHMN_00275 0.53 +2.6
55,717 + gspF OKFHMN_00275 0.57 +0.6
55,768 + gspF OKFHMN_00275 0.62 -0.1
55,768 + gspF OKFHMN_00275 0.62 -0.4
55,768 + gspF OKFHMN_00275 0.62 +0.2
55,923 + gspF OKFHMN_00275 0.74 -2.0

Or see this region's nucleotide sequence