Experiment: EMM_noCarbon with Sucrose and Ammonium Sulfate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF60 and GFF61 are separated by 46 nucleotides GFF61 and GFF62 are separated by 139 nucleotides GFF62 and GFF63 are separated by 105 nucleotides GFF63 and GFF64 are separated by 93 nucleotides GFF64 and GFF65 are separated by 120 nucleotides
GFF60 - Pyruvate,phosphate dikinase (EC 2.7.9.1), at 50,420 to 53,119
GFF60
GFF61 - hypothetical protein, at 53,166 to 53,657
GFF61
GFF62 - tRNA-Arg-ACG, at 53,797 to 53,873
GFF62
GFF63 - hypothetical protein, at 53,979 to 54,158
GFF63
GFF64 - hypothetical protein, at 54,252 to 54,434
GFF64
GFF65 - tRNA-Arg-ACG, at 54,555 to 54,631
GFF65
Position (kb)
53
54 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 52.168 kb on - strand, within GFF60 at 52.171 kb on + strand, within GFF60 at 52.171 kb on + strand, within GFF60 at 52.171 kb on + strand, within GFF60 at 52.171 kb on + strand, within GFF60 at 52.171 kb on + strand, within GFF60 at 52.171 kb on + strand, within GFF60 at 52.171 kb on + strand, within GFF60 at 52.171 kb on + strand, within GFF60 at 52.171 kb on + strand, within GFF60 at 52.171 kb on + strand, within GFF60 at 52.171 kb on + strand, within GFF60 at 52.174 kb on - strand, within GFF60 at 52.179 kb on - strand, within GFF60 at 52.179 kb on - strand, within GFF60 at 52.179 kb on - strand, within GFF60 at 52.179 kb on - strand, within GFF60 at 52.179 kb on - strand, within GFF60 at 52.179 kb on - strand, within GFF60 at 52.179 kb on - strand, within GFF60 at 52.179 kb on - strand, within GFF60 at 52.179 kb on - strand, within GFF60 at 52.179 kb on - strand, within GFF60 at 52.179 kb on - strand, within GFF60 at 52.181 kb on + strand, within GFF60 at 52.181 kb on + strand, within GFF60 at 52.187 kb on + strand, within GFF60 at 52.189 kb on - strand, within GFF60 at 52.202 kb on + strand, within GFF60 at 52.209 kb on + strand, within GFF60 at 52.210 kb on - strand, within GFF60 at 52.210 kb on - strand, within GFF60 at 52.212 kb on - strand, within GFF60 at 52.215 kb on - strand, within GFF60 at 52.217 kb on - strand, within GFF60 at 52.224 kb on + strand, within GFF60 at 52.224 kb on + strand, within GFF60 at 52.224 kb on + strand, within GFF60 at 52.282 kb on - strand, within GFF60 at 52.306 kb on + strand, within GFF60 at 52.316 kb on - strand, within GFF60 at 52.320 kb on + strand, within GFF60 at 52.338 kb on + strand, within GFF60 at 52.338 kb on - strand, within GFF60 at 52.338 kb on - strand, within GFF60 at 52.338 kb on - strand, within GFF60 at 52.339 kb on - strand, within GFF60 at 52.346 kb on - strand, within GFF60 at 52.346 kb on - strand, within GFF60 at 52.348 kb on + strand, within GFF60 at 52.403 kb on + strand, within GFF60 at 52.405 kb on + strand, within GFF60 at 52.405 kb on + strand, within GFF60 at 52.405 kb on + strand, within GFF60 at 52.405 kb on + strand, within GFF60 at 52.411 kb on + strand, within GFF60 at 52.413 kb on - strand, within GFF60 at 52.418 kb on + strand, within GFF60 at 52.425 kb on + strand, within GFF60 at 52.425 kb on + strand, within GFF60 at 52.425 kb on + strand, within GFF60 at 52.457 kb on - strand, within GFF60 at 52.483 kb on - strand, within GFF60 at 52.502 kb on + strand, within GFF60 at 52.510 kb on - strand, within GFF60 at 52.512 kb on + strand, within GFF60 at 52.522 kb on - strand, within GFF60 at 52.529 kb on + strand, within GFF60 at 52.546 kb on + strand, within GFF60 at 52.546 kb on + strand, within GFF60 at 52.552 kb on - strand, within GFF60 at 52.554 kb on - strand, within GFF60 at 52.554 kb on - strand, within GFF60 at 52.561 kb on + strand, within GFF60 at 52.576 kb on + strand, within GFF60 at 52.579 kb on - strand, within GFF60 at 52.579 kb on - strand, within GFF60 at 52.601 kb on + strand, within GFF60 at 52.634 kb on - strand, within GFF60 at 52.640 kb on - strand, within GFF60 at 52.642 kb on + strand, within GFF60 at 52.742 kb on + strand, within GFF60 at 52.742 kb on + strand, within GFF60 at 52.750 kb on - strand, within GFF60 at 52.752 kb on - strand, within GFF60 at 52.794 kb on + strand, within GFF60 at 52.794 kb on + strand, within GFF60 at 52.794 kb on + strand, within GFF60 at 52.794 kb on - strand, within GFF60 at 52.835 kb on - strand, within GFF60 at 52.854 kb on + strand at 52.894 kb on + strand at 52.914 kb on + strand at 52.922 kb on - strand at 52.927 kb on - strand at 52.974 kb on + strand at 52.986 kb on + strand at 53.062 kb on - strand at 53.062 kb on - strand at 53.064 kb on + strand at 53.092 kb on + strand at 53.134 kb on + strand at 53.139 kb on + strand at 53.164 kb on + strand at 53.164 kb on + strand at 53.164 kb on + strand at 53.164 kb on + strand at 53.164 kb on + strand at 53.164 kb on + strand at 53.164 kb on + strand at 53.164 kb on + strand at 53.164 kb on + strand at 53.164 kb on + strand at 53.164 kb on + strand at 53.164 kb on + strand at 53.164 kb on + strand at 53.164 kb on + strand at 53.169 kb on + strand at 53.171 kb on - strand at 53.172 kb on - strand at 53.172 kb on - strand at 53.172 kb on - strand at 53.172 kb on - strand at 53.172 kb on - strand at 53.172 kb on - strand at 53.172 kb on - strand at 53.177 kb on - strand at 53.177 kb on - strand at 53.195 kb on + strand at 53.195 kb on - strand at 53.241 kb on + strand, within GFF61 at 53.244 kb on - strand, within GFF61 at 53.249 kb on - strand, within GFF61 at 53.249 kb on - strand, within GFF61 at 53.288 kb on - strand, within GFF61 at 53.307 kb on + strand, within GFF61 at 53.342 kb on - strand, within GFF61 at 53.391 kb on + strand, within GFF61 at 53.399 kb on - strand, within GFF61 at 53.414 kb on - strand, within GFF61 at 53.414 kb on - strand, within GFF61 at 53.502 kb on - strand, within GFF61 at 53.517 kb on - strand, within GFF61 at 53.524 kb on + strand, within GFF61 at 53.528 kb on + strand, within GFF61 at 53.531 kb on - strand, within GFF61 at 53.532 kb on - strand, within GFF61 at 53.538 kb on + strand, within GFF61 at 53.543 kb on + strand, within GFF61 at 53.598 kb on + strand, within GFF61 at 53.605 kb on + strand, within GFF61 at 53.605 kb on - strand, within GFF61 at 53.607 kb on + strand, within GFF61 at 53.617 kb on + strand at 53.622 kb on - strand at 53.628 kb on - strand at 53.628 kb on - strand at 53.630 kb on + strand at 53.693 kb on + strand at 53.701 kb on - strand at 53.712 kb on + strand at 53.727 kb on - strand at 53.792 kb on - strand at 53.812 kb on - strand, within GFF62 at 53.812 kb on - strand, within GFF62 at 53.819 kb on - strand, within GFF62 at 53.902 kb on - strand at 53.907 kb on + strand at 53.977 kb on - strand at 53.981 kb on + strand at 53.988 kb on + strand at 53.988 kb on + strand at 53.988 kb on + strand at 53.988 kb on + strand at 53.988 kb on + strand at 53.988 kb on + strand at 53.996 kb on - strand at 54.010 kb on + strand, within GFF63 at 54.022 kb on + strand, within GFF63 at 54.035 kb on + strand, within GFF63 at 54.035 kb on + strand, within GFF63 at 54.035 kb on + strand, within GFF63 at 54.043 kb on - strand, within GFF63 at 54.044 kb on - strand, within GFF63 at 54.075 kb on + strand, within GFF63 at 54.092 kb on + strand, within GFF63 at 54.105 kb on + strand, within GFF63 at 54.113 kb on - strand, within GFF63 at 54.113 kb on - strand, within GFF63 at 54.119 kb on + strand, within GFF63 at 54.119 kb on + strand, within GFF63 at 54.122 kb on - strand, within GFF63 at 54.127 kb on - strand, within GFF63 at 54.159 kb on + strand at 54.176 kb on - strand at 54.198 kb on + strand at 54.211 kb on + strand at 54.259 kb on + strand at 54.259 kb on + strand at 54.262 kb on - strand at 54.301 kb on - strand, within GFF64 at 54.337 kb on - strand, within GFF64 at 54.337 kb on - strand, within GFF64 at 54.367 kb on - strand, within GFF64 at 54.369 kb on + strand, within GFF64 at 54.374 kb on + strand, within GFF64 at 54.428 kb on + strand at 54.436 kb on - strand at 54.532 kb on + strand at 54.548 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with Sucrose and Ammonium Sulfate remove 52,168 - GFF60 0.65 -1.7 52,171 + GFF60 0.65 -0.9 52,171 + GFF60 0.65 -1.6 52,171 + GFF60 0.65 -2.4 52,171 + GFF60 0.65 -0.4 52,171 + GFF60 0.65 -2.0 52,171 + GFF60 0.65 -1.7 52,171 + GFF60 0.65 -1.7 52,171 + GFF60 0.65 -1.6 52,171 + GFF60 0.65 -1.1 52,171 + GFF60 0.65 -2.3 52,171 + GFF60 0.65 -0.8 52,174 - GFF60 0.65 -1.5 52,179 - GFF60 0.65 -1.7 52,179 - GFF60 0.65 -0.3 52,179 - GFF60 0.65 -2.6 52,179 - GFF60 0.65 +1.4 52,179 - GFF60 0.65 -1.0 52,179 - GFF60 0.65 -2.1 52,179 - GFF60 0.65 -0.1 52,179 - GFF60 0.65 +0.5 52,179 - GFF60 0.65 -0.7 52,179 - GFF60 0.65 -1.3 52,179 - GFF60 0.65 -2.1 52,181 + GFF60 0.65 -2.3 52,181 + GFF60 0.65 -2.3 52,187 + GFF60 0.65 -0.4 52,189 - GFF60 0.66 -1.2 52,202 + GFF60 0.66 -2.5 52,209 + GFF60 0.66 -1.8 52,210 - GFF60 0.66 -2.9 52,210 - GFF60 0.66 -1.6 52,212 - GFF60 0.66 -1.4 52,215 - GFF60 0.66 -2.9 52,217 - GFF60 0.67 +0.5 52,224 + GFF60 0.67 -2.3 52,224 + GFF60 0.67 -2.4 52,224 + GFF60 0.67 -1.6 52,282 - GFF60 0.69 -0.3 52,306 + GFF60 0.70 -2.1 52,316 - GFF60 0.70 -1.3 52,320 + GFF60 0.70 -1.2 52,338 + GFF60 0.71 -1.9 52,338 - GFF60 0.71 -1.1 52,338 - GFF60 0.71 -1.1 52,338 - GFF60 0.71 -0.4 52,339 - GFF60 0.71 -0.1 52,346 - GFF60 0.71 +0.3 52,346 - GFF60 0.71 -2.0 52,348 + GFF60 0.71 -3.5 52,403 + GFF60 0.73 -2.1 52,405 + GFF60 0.74 -1.5 52,405 + GFF60 0.74 -0.4 52,405 + GFF60 0.74 +0.4 52,405 + GFF60 0.74 -1.9 52,411 + GFF60 0.74 -0.2 52,413 - GFF60 0.74 +0.1 52,418 + GFF60 0.74 -2.7 52,425 + GFF60 0.74 -1.3 52,425 + GFF60 0.74 -3.5 52,425 + GFF60 0.74 -1.4 52,457 - GFF60 0.75 -2.6 52,483 - GFF60 0.76 -2.2 52,502 + GFF60 0.77 -2.5 52,510 - GFF60 0.77 -2.6 52,512 + GFF60 0.77 -1.7 52,522 - GFF60 0.78 -1.4 52,529 + GFF60 0.78 -1.5 52,546 + GFF60 0.79 -0.5 52,546 + GFF60 0.79 -2.5 52,552 - GFF60 0.79 -2.5 52,554 - GFF60 0.79 -1.4 52,554 - GFF60 0.79 -0.9 52,561 + GFF60 0.79 -1.6 52,576 + GFF60 0.80 -0.1 52,579 - GFF60 0.80 +0.5 52,579 - GFF60 0.80 -1.6 52,601 + GFF60 0.81 -1.7 52,634 - GFF60 0.82 -0.2 52,640 - GFF60 0.82 -2.4 52,642 + GFF60 0.82 -1.4 52,742 + GFF60 0.86 -1.6 52,742 + GFF60 0.86 -2.5 52,750 - GFF60 0.86 +0.4 52,752 - GFF60 0.86 -1.3 52,794 + GFF60 0.88 -1.4 52,794 + GFF60 0.88 +0.2 52,794 + GFF60 0.88 -2.2 52,794 - GFF60 0.88 -2.6 52,835 - GFF60 0.89 -1.7 52,854 + -1.0 52,894 + -1.5 52,914 + -1.2 52,922 - -2.0 52,927 - -2.5 52,974 + -2.1 52,986 + -1.6 53,062 - -1.9 53,062 - -1.5 53,064 + -0.1 53,092 + -0.9 53,134 + +1.0 53,139 + -0.7 53,164 + -0.2 53,164 + -0.4 53,164 + -0.9 53,164 + +0.2 53,164 + +0.5 53,164 + +0.2 53,164 + -0.5 53,164 + -0.4 53,164 + +0.1 53,164 + +0.1 53,164 + +0.6 53,164 + +0.6 53,164 + +0.3 53,164 + +0.7 53,169 + +0.3 53,171 - -1.5 53,172 - +1.1 53,172 - -0.6 53,172 - -0.2 53,172 - +0.5 53,172 - -0.2 53,172 - +0.8 53,172 - -1.9 53,177 - -0.4 53,177 - -0.3 53,195 + -1.1 53,195 - -0.6 53,241 + GFF61 0.15 +0.5 53,244 - GFF61 0.16 -0.4 53,249 - GFF61 0.17 -0.9 53,249 - GFF61 0.17 +0.6 53,288 - GFF61 0.25 -1.1 53,307 + GFF61 0.29 +0.0 53,342 - GFF61 0.36 -0.1 53,391 + GFF61 0.46 -0.2 53,399 - GFF61 0.47 -0.7 53,414 - GFF61 0.50 -0.9 53,414 - GFF61 0.50 -0.8 53,502 - GFF61 0.68 +0.6 53,517 - GFF61 0.71 -0.2 53,524 + GFF61 0.73 +0.1 53,528 + GFF61 0.74 -1.3 53,531 - GFF61 0.74 -0.5 53,532 - GFF61 0.74 +0.4 53,538 + GFF61 0.76 -0.5 53,543 + GFF61 0.77 +0.8 53,598 + GFF61 0.88 +0.2 53,605 + GFF61 0.89 -0.7 53,605 - GFF61 0.89 -2.6 53,607 + GFF61 0.90 -1.2 53,617 + -1.0 53,622 - +0.5 53,628 - -0.5 53,628 - -0.3 53,630 + +0.7 53,693 + +0.5 53,701 - -0.0 53,712 + +0.3 53,727 - +0.3 53,792 - +1.1 53,812 - GFF62 0.19 +1.4 53,812 - GFF62 0.19 +0.5 53,819 - GFF62 0.29 -0.2 53,902 - +0.1 53,907 + -0.1 53,977 - +0.5 53,981 + +0.3 53,988 + +0.5 53,988 + +1.5 53,988 + -0.1 53,988 + +0.9 53,988 + -0.5 53,988 + -0.2 53,996 - -1.4 54,010 + GFF63 0.17 +1.0 54,022 + GFF63 0.24 +0.7 54,035 + GFF63 0.31 -0.7 54,035 + GFF63 0.31 +1.7 54,035 + GFF63 0.31 +0.7 54,043 - GFF63 0.36 -0.9 54,044 - GFF63 0.36 +0.9 54,075 + GFF63 0.53 +0.1 54,092 + GFF63 0.63 +1.0 54,105 + GFF63 0.70 -1.0 54,113 - GFF63 0.74 +0.3 54,113 - GFF63 0.74 -2.5 54,119 + GFF63 0.78 -1.5 54,119 + GFF63 0.78 +2.0 54,122 - GFF63 0.79 -0.4 54,127 - GFF63 0.82 -1.3 54,159 + -0.1 54,176 - -0.2 54,198 + -0.7 54,211 + -1.0 54,259 + -0.3 54,259 + -0.2 54,262 - -0.5 54,301 - GFF64 0.27 -0.7 54,337 - GFF64 0.46 -1.2 54,337 - GFF64 0.46 -1.5 54,367 - GFF64 0.63 -3.6 54,369 + GFF64 0.64 -1.1 54,374 + GFF64 0.67 -0.4 54,428 + -1.6 54,436 - -0.8 54,532 + -1.7 54,548 + -2.2
Or see this region's nucleotide sequence