Experiment: EMM_noCarbon with Sucrose and Ammonium Sulfate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3795 and GFF3796 overlap by 1 nucleotides GFF3796 and GFF3797 overlap by 20 nucleotides GFF3797 and GFF3798 are separated by 52 nucleotides GFF3798 and GFF3799 are separated by 90 nucleotides
GFF3795 - Diaminopimelate decarboxylase (EC 4.1.1.20), at 3,836,763 to 3,838,028
GFF3795
GFF3796 - Precorrin-2 oxidase (EC 1.3.1.76) @ Sirohydrochlorin ferrochelatase activity of CysG (EC 4.99.1.4) / Uroporphyrinogen-III methyltransferase (EC 2.1.1.107), at 3,838,028 to 3,838,786
GFF3796
GFF3797 - hypothetical protein, at 3,838,767 to 3,839,390
GFF3797
GFF3798 - Transcriptional regulator, AcrR family, at 3,839,443 to 3,840,096
GFF3798
GFF3799 - Outer membrane factor (OMF) lipoprotein associated wth EmrAB-OMF efflux system, at 3,840,187 to 3,841,701
GFF3799
Position (kb)
3838
3839
3840 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3837.779 kb on + strand, within GFF3795 at 3837.787 kb on - strand, within GFF3795 at 3837.799 kb on - strand, within GFF3795 at 3837.813 kb on + strand, within GFF3795 at 3837.826 kb on + strand, within GFF3795 at 3837.836 kb on + strand, within GFF3795 at 3837.838 kb on - strand, within GFF3795 at 3837.844 kb on + strand, within GFF3795 at 3837.852 kb on + strand, within GFF3795 at 3837.854 kb on + strand, within GFF3795 at 3837.883 kb on + strand, within GFF3795 at 3837.901 kb on + strand, within GFF3795 at 3837.922 kb on + strand at 3837.922 kb on + strand at 3837.922 kb on + strand at 3837.922 kb on + strand at 3837.922 kb on + strand at 3837.924 kb on + strand at 3837.930 kb on - strand at 3837.930 kb on - strand at 3837.930 kb on - strand at 3837.952 kb on + strand at 3837.960 kb on - strand at 3838.025 kb on + strand at 3838.033 kb on - strand at 3838.758 kb on + strand at 3838.760 kb on + strand at 3838.760 kb on + strand at 3838.770 kb on + strand at 3838.770 kb on + strand at 3838.770 kb on + strand at 3838.770 kb on + strand at 3838.778 kb on - strand at 3838.778 kb on - strand at 3838.778 kb on - strand at 3838.778 kb on - strand at 3838.785 kb on + strand at 3838.803 kb on - strand at 3838.809 kb on + strand at 3838.814 kb on + strand at 3838.814 kb on + strand at 3838.817 kb on - strand at 3838.817 kb on - strand at 3838.822 kb on - strand at 3838.827 kb on - strand at 3838.844 kb on + strand, within GFF3797 at 3838.845 kb on - strand, within GFF3797 at 3838.870 kb on - strand, within GFF3797 at 3838.878 kb on + strand, within GFF3797 at 3838.880 kb on + strand, within GFF3797 at 3838.880 kb on + strand, within GFF3797 at 3838.880 kb on + strand, within GFF3797 at 3838.888 kb on - strand, within GFF3797 at 3838.888 kb on - strand, within GFF3797 at 3838.913 kb on - strand, within GFF3797 at 3838.918 kb on + strand, within GFF3797 at 3838.926 kb on - strand, within GFF3797 at 3838.956 kb on + strand, within GFF3797 at 3838.973 kb on + strand, within GFF3797 at 3838.973 kb on + strand, within GFF3797 at 3838.988 kb on + strand, within GFF3797 at 3838.996 kb on - strand, within GFF3797 at 3838.996 kb on - strand, within GFF3797 at 3839.023 kb on + strand, within GFF3797 at 3839.031 kb on + strand, within GFF3797 at 3839.031 kb on + strand, within GFF3797 at 3839.031 kb on + strand, within GFF3797 at 3839.037 kb on - strand, within GFF3797 at 3839.070 kb on + strand, within GFF3797 at 3839.073 kb on - strand, within GFF3797 at 3839.088 kb on + strand, within GFF3797 at 3839.096 kb on - strand, within GFF3797 at 3839.098 kb on - strand, within GFF3797 at 3839.144 kb on + strand, within GFF3797 at 3839.153 kb on + strand, within GFF3797 at 3839.153 kb on + strand, within GFF3797 at 3839.153 kb on + strand, within GFF3797 at 3839.153 kb on + strand, within GFF3797 at 3839.161 kb on - strand, within GFF3797 at 3839.166 kb on - strand, within GFF3797 at 3839.168 kb on + strand, within GFF3797 at 3839.168 kb on + strand, within GFF3797 at 3839.168 kb on + strand, within GFF3797 at 3839.168 kb on + strand, within GFF3797 at 3839.168 kb on + strand, within GFF3797 at 3839.168 kb on + strand, within GFF3797 at 3839.174 kb on + strand, within GFF3797 at 3839.176 kb on - strand, within GFF3797 at 3839.176 kb on - strand, within GFF3797 at 3839.176 kb on - strand, within GFF3797 at 3839.192 kb on + strand, within GFF3797 at 3839.198 kb on + strand, within GFF3797 at 3839.199 kb on + strand, within GFF3797 at 3839.199 kb on + strand, within GFF3797 at 3839.236 kb on - strand, within GFF3797 at 3839.239 kb on + strand, within GFF3797 at 3839.242 kb on - strand, within GFF3797 at 3839.262 kb on + strand, within GFF3797 at 3839.270 kb on - strand, within GFF3797 at 3839.281 kb on + strand, within GFF3797 at 3839.303 kb on - strand, within GFF3797 at 3839.353 kb on - strand at 3839.360 kb on + strand at 3839.363 kb on - strand at 3839.367 kb on + strand at 3839.368 kb on - strand at 3839.416 kb on + strand at 3839.416 kb on + strand at 3839.417 kb on + strand at 3839.424 kb on - strand at 3839.424 kb on - strand at 3839.471 kb on - strand at 3839.486 kb on - strand at 3839.490 kb on - strand at 3839.564 kb on - strand, within GFF3798 at 3839.564 kb on - strand, within GFF3798 at 3839.566 kb on + strand, within GFF3798 at 3839.584 kb on + strand, within GFF3798 at 3839.600 kb on + strand, within GFF3798 at 3839.681 kb on - strand, within GFF3798 at 3839.733 kb on - strand, within GFF3798 at 3839.770 kb on - strand, within GFF3798 at 3839.790 kb on + strand, within GFF3798 at 3839.790 kb on + strand, within GFF3798 at 3839.791 kb on + strand, within GFF3798 at 3839.798 kb on - strand, within GFF3798 at 3839.801 kb on + strand, within GFF3798 at 3839.803 kb on + strand, within GFF3798 at 3839.813 kb on + strand, within GFF3798 at 3839.813 kb on + strand, within GFF3798 at 3839.813 kb on + strand, within GFF3798 at 3839.813 kb on + strand, within GFF3798 at 3839.821 kb on - strand, within GFF3798 at 3839.821 kb on - strand, within GFF3798 at 3839.821 kb on - strand, within GFF3798 at 3839.821 kb on - strand, within GFF3798 at 3839.821 kb on - strand, within GFF3798 at 3839.821 kb on - strand, within GFF3798 at 3839.821 kb on - strand, within GFF3798 at 3839.821 kb on - strand, within GFF3798 at 3839.821 kb on - strand, within GFF3798 at 3839.843 kb on - strand, within GFF3798 at 3839.937 kb on + strand, within GFF3798 at 3839.937 kb on + strand, within GFF3798 at 3839.945 kb on - strand, within GFF3798 at 3840.065 kb on - strand at 3840.101 kb on + strand at 3840.106 kb on + strand at 3840.128 kb on + strand at 3840.134 kb on + strand at 3840.134 kb on + strand at 3840.134 kb on + strand at 3840.134 kb on + strand at 3840.134 kb on + strand at 3840.134 kb on + strand at 3840.169 kb on + strand at 3840.177 kb on - strand at 3840.178 kb on + strand at 3840.179 kb on + strand at 3840.189 kb on + strand at 3840.218 kb on + strand at 3840.227 kb on + strand at 3840.235 kb on - strand at 3840.241 kb on + strand at 3840.241 kb on + strand at 3840.246 kb on + strand at 3840.254 kb on - strand at 3840.263 kb on - strand at 3840.265 kb on - strand at 3840.319 kb on + strand at 3840.329 kb on + strand at 3840.388 kb on + strand, within GFF3799
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with Sucrose and Ammonium Sulfate remove 3,837,779 + GFF3795 0.80 -3.8 3,837,787 - GFF3795 0.81 -1.5 3,837,799 - GFF3795 0.82 -2.2 3,837,813 + GFF3795 0.83 -2.3 3,837,826 + GFF3795 0.84 -2.2 3,837,836 + GFF3795 0.85 -1.8 3,837,838 - GFF3795 0.85 -2.5 3,837,844 + GFF3795 0.85 -1.8 3,837,852 + GFF3795 0.86 -3.1 3,837,854 + GFF3795 0.86 -1.7 3,837,883 + GFF3795 0.88 -1.8 3,837,901 + GFF3795 0.90 -1.2 3,837,922 + -1.9 3,837,922 + -3.2 3,837,922 + -1.7 3,837,922 + -3.1 3,837,922 + -1.3 3,837,924 + -2.8 3,837,930 - -2.2 3,837,930 - -1.8 3,837,930 - -3.8 3,837,952 + -1.8 3,837,960 - -2.0 3,838,025 + -1.5 3,838,033 - +0.8 3,838,758 + -0.6 3,838,760 + -2.7 3,838,760 + -2.0 3,838,770 + -1.6 3,838,770 + -1.6 3,838,770 + +0.2 3,838,770 + -0.3 3,838,778 - -1.9 3,838,778 - -1.4 3,838,778 - -1.5 3,838,778 - -0.4 3,838,785 + -1.6 3,838,803 - -0.2 3,838,809 + -0.2 3,838,814 + -1.5 3,838,814 + -0.6 3,838,817 - -0.3 3,838,817 - +0.4 3,838,822 - +0.1 3,838,827 - -2.2 3,838,844 + GFF3797 0.12 -0.1 3,838,845 - GFF3797 0.12 -0.2 3,838,870 - GFF3797 0.17 -0.6 3,838,878 + GFF3797 0.18 +0.7 3,838,880 + GFF3797 0.18 +1.9 3,838,880 + GFF3797 0.18 -0.7 3,838,880 + GFF3797 0.18 -0.3 3,838,888 - GFF3797 0.19 +0.3 3,838,888 - GFF3797 0.19 -0.6 3,838,913 - GFF3797 0.23 -0.6 3,838,918 + GFF3797 0.24 -0.1 3,838,926 - GFF3797 0.25 -1.3 3,838,956 + GFF3797 0.30 -0.3 3,838,973 + GFF3797 0.33 -0.9 3,838,973 + GFF3797 0.33 -0.2 3,838,988 + GFF3797 0.35 -0.7 3,838,996 - GFF3797 0.37 -0.2 3,838,996 - GFF3797 0.37 +0.2 3,839,023 + GFF3797 0.41 -2.2 3,839,031 + GFF3797 0.42 -0.9 3,839,031 + GFF3797 0.42 -1.6 3,839,031 + GFF3797 0.42 -2.0 3,839,037 - GFF3797 0.43 +0.7 3,839,070 + GFF3797 0.49 -3.5 3,839,073 - GFF3797 0.49 +1.3 3,839,088 + GFF3797 0.51 -0.1 3,839,096 - GFF3797 0.53 -0.5 3,839,098 - GFF3797 0.53 -1.0 3,839,144 + GFF3797 0.60 -1.2 3,839,153 + GFF3797 0.62 +1.9 3,839,153 + GFF3797 0.62 -1.6 3,839,153 + GFF3797 0.62 -0.2 3,839,153 + GFF3797 0.62 -0.8 3,839,161 - GFF3797 0.63 -0.8 3,839,166 - GFF3797 0.64 +0.9 3,839,168 + GFF3797 0.64 -0.4 3,839,168 + GFF3797 0.64 -0.3 3,839,168 + GFF3797 0.64 +0.2 3,839,168 + GFF3797 0.64 +0.3 3,839,168 + GFF3797 0.64 -0.2 3,839,168 + GFF3797 0.64 -1.2 3,839,174 + GFF3797 0.65 -1.9 3,839,176 - GFF3797 0.66 -0.8 3,839,176 - GFF3797 0.66 -0.6 3,839,176 - GFF3797 0.66 -0.3 3,839,192 + GFF3797 0.68 -1.5 3,839,198 + GFF3797 0.69 -2.0 3,839,199 + GFF3797 0.69 -1.5 3,839,199 + GFF3797 0.69 -1.0 3,839,236 - GFF3797 0.75 -1.7 3,839,239 + GFF3797 0.76 -0.3 3,839,242 - GFF3797 0.76 +0.9 3,839,262 + GFF3797 0.79 -0.0 3,839,270 - GFF3797 0.81 -0.7 3,839,281 + GFF3797 0.82 -0.4 3,839,303 - GFF3797 0.86 -0.1 3,839,353 - -1.1 3,839,360 + +0.9 3,839,363 - -0.2 3,839,367 + -0.3 3,839,368 - -0.6 3,839,416 + -2.3 3,839,416 + -0.3 3,839,417 + -0.2 3,839,424 - +0.3 3,839,424 - +0.2 3,839,471 - +1.8 3,839,486 - +1.5 3,839,490 - -1.3 3,839,564 - GFF3798 0.19 +1.0 3,839,564 - GFF3798 0.19 -1.7 3,839,566 + GFF3798 0.19 -1.3 3,839,584 + GFF3798 0.22 +0.0 3,839,600 + GFF3798 0.24 -1.5 3,839,681 - GFF3798 0.36 -0.2 3,839,733 - GFF3798 0.44 -0.1 3,839,770 - GFF3798 0.50 -0.5 3,839,790 + GFF3798 0.53 -1.5 3,839,790 + GFF3798 0.53 -0.7 3,839,791 + GFF3798 0.53 -1.7 3,839,798 - GFF3798 0.54 +1.8 3,839,801 + GFF3798 0.55 +0.7 3,839,803 + GFF3798 0.55 +0.4 3,839,813 + GFF3798 0.57 +0.8 3,839,813 + GFF3798 0.57 -0.6 3,839,813 + GFF3798 0.57 -1.7 3,839,813 + GFF3798 0.57 -1.4 3,839,821 - GFF3798 0.58 +0.7 3,839,821 - GFF3798 0.58 -1.4 3,839,821 - GFF3798 0.58 -1.1 3,839,821 - GFF3798 0.58 -0.5 3,839,821 - GFF3798 0.58 -0.7 3,839,821 - GFF3798 0.58 -1.2 3,839,821 - GFF3798 0.58 -0.1 3,839,821 - GFF3798 0.58 -0.2 3,839,821 - GFF3798 0.58 +1.2 3,839,843 - GFF3798 0.61 +0.1 3,839,937 + GFF3798 0.76 -1.3 3,839,937 + GFF3798 0.76 -0.6 3,839,945 - GFF3798 0.77 +1.2 3,840,065 - -0.0 3,840,101 + -0.5 3,840,106 + +0.8 3,840,128 + -1.5 3,840,134 + +1.1 3,840,134 + +0.0 3,840,134 + -2.6 3,840,134 + +1.1 3,840,134 + +1.0 3,840,134 + -2.3 3,840,169 + -1.7 3,840,177 - -1.8 3,840,178 + +0.7 3,840,179 + -0.4 3,840,189 + +0.7 3,840,218 + -1.5 3,840,227 + -1.1 3,840,235 - +0.7 3,840,241 + -0.7 3,840,241 + -0.4 3,840,246 + +0.4 3,840,254 - +0.3 3,840,263 - +0.1 3,840,265 - +0.8 3,840,319 + +0.7 3,840,329 + -1.3 3,840,388 + GFF3799 0.13 +0.4
Or see this region's nucleotide sequence