Experiment: EMM_noCarbon with Sucrose and Ammonium Sulfate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF260 and GFF261 overlap by 4 nucleotides GFF261 and GFF262 overlap by 4 nucleotides GFF262 and GFF263 are separated by 79 nucleotides GFF263 and GFF264 overlap by 4 nucleotides GFF264 and GFF265 are separated by 111 nucleotides GFF265 and GFF266 are separated by 19 nucleotides
GFF260 - hypothetical protein, at 261,254 to 261,448
GFF260
GFF261 - hypothetical protein, at 261,445 to 261,657
GFF261
GFF262 - hypothetical protein, at 261,654 to 261,956
GFF262
GFF263 - hypothetical protein, at 262,036 to 262,371
GFF263
GFF264 - hypothetical protein, at 262,368 to 262,565
GFF264
GFF265 - hypothetical protein, at 262,677 to 263,429
GFF265
GFF266 - hypothetical protein, at 263,449 to 263,685
GFF266
Position (kb) 262
263 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 261.387 kb on + strand, within GFF260 at 261.393 kb on + strand, within GFF260 at 261.393 kb on + strand, within GFF260 at 261.395 kb on - strand, within GFF260 at 261.398 kb on + strand, within GFF260 at 261.401 kb on - strand, within GFF260 at 261.411 kb on - strand, within GFF260 at 261.431 kb on + strand at 261.439 kb on - strand at 261.439 kb on - strand at 261.443 kb on + strand at 261.443 kb on + strand at 261.443 kb on + strand at 261.451 kb on - strand at 261.451 kb on - strand at 261.451 kb on - strand at 261.451 kb on - strand at 261.451 kb on - strand at 261.451 kb on - strand at 261.468 kb on + strand, within GFF261 at 261.468 kb on + strand, within GFF261 at 261.487 kb on + strand, within GFF261 at 261.487 kb on + strand, within GFF261 at 261.497 kb on - strand, within GFF261 at 261.497 kb on - strand, within GFF261 at 261.519 kb on + strand, within GFF261 at 261.519 kb on + strand, within GFF261 at 261.519 kb on + strand, within GFF261 at 261.527 kb on - strand, within GFF261 at 261.532 kb on - strand, within GFF261 at 261.542 kb on + strand, within GFF261 at 261.545 kb on - strand, within GFF261 at 261.552 kb on + strand, within GFF261 at 261.555 kb on - strand, within GFF261 at 261.559 kb on + strand, within GFF261 at 261.559 kb on + strand, within GFF261 at 261.559 kb on + strand, within GFF261 at 261.560 kb on - strand, within GFF261 at 261.576 kb on + strand, within GFF261 at 261.583 kb on + strand, within GFF261 at 261.583 kb on + strand, within GFF261 at 261.583 kb on + strand, within GFF261 at 261.591 kb on - strand, within GFF261 at 261.591 kb on - strand, within GFF261 at 261.599 kb on + strand, within GFF261 at 261.603 kb on + strand, within GFF261 at 261.603 kb on + strand, within GFF261 at 261.603 kb on + strand, within GFF261 at 261.603 kb on + strand, within GFF261 at 261.658 kb on - strand at 261.668 kb on - strand at 261.678 kb on - strand at 261.678 kb on - strand at 261.720 kb on + strand, within GFF262 at 261.724 kb on + strand, within GFF262 at 261.724 kb on + strand, within GFF262 at 261.731 kb on - strand, within GFF262 at 261.739 kb on + strand, within GFF262 at 261.742 kb on - strand, within GFF262 at 261.767 kb on + strand, within GFF262 at 261.796 kb on + strand, within GFF262 at 261.798 kb on + strand, within GFF262 at 261.824 kb on + strand, within GFF262 at 261.824 kb on + strand, within GFF262 at 261.829 kb on + strand, within GFF262 at 261.829 kb on + strand, within GFF262 at 261.832 kb on - strand, within GFF262 at 261.832 kb on - strand, within GFF262 at 261.832 kb on - strand, within GFF262 at 261.832 kb on - strand, within GFF262 at 261.832 kb on - strand, within GFF262 at 261.848 kb on + strand, within GFF262 at 261.911 kb on - strand, within GFF262 at 261.914 kb on - strand, within GFF262 at 261.928 kb on - strand at 261.945 kb on - strand at 261.950 kb on + strand at 261.950 kb on + strand at 261.958 kb on - strand at 261.958 kb on - strand at 261.958 kb on - strand at 261.958 kb on - strand at 261.960 kb on - strand at 261.966 kb on - strand at 261.966 kb on - strand at 261.966 kb on - strand at 261.986 kb on + strand at 261.986 kb on + strand at 261.996 kb on + strand at 262.003 kb on + strand at 262.003 kb on + strand at 262.011 kb on - strand at 262.049 kb on - strand at 262.066 kb on + strand at 262.073 kb on + strand, within GFF263 at 262.073 kb on + strand, within GFF263 at 262.074 kb on - strand, within GFF263 at 262.078 kb on + strand, within GFF263 at 262.081 kb on - strand, within GFF263 at 262.086 kb on - strand, within GFF263 at 262.086 kb on - strand, within GFF263 at 262.086 kb on - strand, within GFF263 at 262.088 kb on + strand, within GFF263 at 262.132 kb on - strand, within GFF263 at 262.134 kb on + strand, within GFF263 at 262.158 kb on + strand, within GFF263 at 262.158 kb on + strand, within GFF263 at 262.158 kb on + strand, within GFF263 at 262.166 kb on - strand, within GFF263 at 262.166 kb on - strand, within GFF263 at 262.166 kb on - strand, within GFF263 at 262.213 kb on + strand, within GFF263 at 262.233 kb on - strand, within GFF263 at 262.244 kb on - strand, within GFF263 at 262.250 kb on + strand, within GFF263 at 262.254 kb on + strand, within GFF263 at 262.336 kb on - strand, within GFF263 at 262.369 kb on - strand at 262.398 kb on + strand, within GFF264 at 262.398 kb on - strand, within GFF264 at 262.405 kb on + strand, within GFF264 at 262.405 kb on + strand, within GFF264 at 262.406 kb on + strand, within GFF264 at 262.414 kb on - strand, within GFF264 at 262.414 kb on - strand, within GFF264 at 262.414 kb on - strand, within GFF264 at 262.416 kb on - strand, within GFF264 at 262.416 kb on - strand, within GFF264 at 262.416 kb on - strand, within GFF264 at 262.416 kb on - strand, within GFF264 at 262.416 kb on - strand, within GFF264 at 262.436 kb on + strand, within GFF264 at 262.441 kb on + strand, within GFF264 at 262.441 kb on + strand, within GFF264 at 262.448 kb on + strand, within GFF264 at 262.448 kb on + strand, within GFF264 at 262.448 kb on + strand, within GFF264 at 262.449 kb on - strand, within GFF264 at 262.463 kb on - strand, within GFF264 at 262.465 kb on + strand, within GFF264 at 262.465 kb on + strand, within GFF264 at 262.465 kb on + strand, within GFF264 at 262.473 kb on - strand, within GFF264 at 262.473 kb on - strand, within GFF264 at 262.473 kb on - strand, within GFF264 at 262.480 kb on + strand, within GFF264 at 262.519 kb on + strand, within GFF264 at 262.535 kb on + strand, within GFF264 at 262.542 kb on - strand, within GFF264 at 262.543 kb on - strand, within GFF264 at 262.544 kb on + strand, within GFF264 at 262.544 kb on + strand, within GFF264 at 262.550 kb on + strand at 262.550 kb on + strand at 262.552 kb on - strand at 262.559 kb on + strand at 262.559 kb on + strand at 262.569 kb on + strand at 262.583 kb on + strand at 262.591 kb on - strand at 262.599 kb on + strand at 262.636 kb on + strand at 262.640 kb on + strand at 262.649 kb on - strand at 262.651 kb on + strand at 262.659 kb on + strand at 262.659 kb on + strand at 262.659 kb on + strand at 262.659 kb on + strand at 262.667 kb on - strand at 262.667 kb on - strand at 262.667 kb on - strand at 262.667 kb on - strand at 262.667 kb on - strand at 262.668 kb on - strand at 262.681 kb on + strand at 262.683 kb on + strand at 262.683 kb on + strand at 262.689 kb on - strand at 262.704 kb on + strand at 262.704 kb on - strand at 262.712 kb on - strand at 262.753 kb on + strand, within GFF265 at 262.755 kb on + strand, within GFF265 at 262.763 kb on - strand, within GFF265 at 262.867 kb on - strand, within GFF265 at 262.890 kb on - strand, within GFF265 at 262.890 kb on - strand, within GFF265 at 262.909 kb on + strand, within GFF265 at 262.910 kb on - strand, within GFF265 at 262.968 kb on + strand, within GFF265 at 262.976 kb on + strand, within GFF265 at 262.994 kb on - strand, within GFF265 at 263.022 kb on + strand, within GFF265 at 263.023 kb on + strand, within GFF265 at 263.053 kb on + strand, within GFF265 at 263.098 kb on + strand, within GFF265 at 263.118 kb on + strand, within GFF265 at 263.131 kb on + strand, within GFF265 at 263.161 kb on + strand, within GFF265 at 263.161 kb on + strand, within GFF265 at 263.161 kb on + strand, within GFF265 at 263.169 kb on - strand, within GFF265 at 263.186 kb on - strand, within GFF265 at 263.186 kb on - strand, within GFF265 at 263.186 kb on - strand, within GFF265 at 263.294 kb on + strand, within GFF265 at 263.294 kb on + strand, within GFF265 at 263.323 kb on - strand, within GFF265 at 263.324 kb on + strand, within GFF265 at 263.329 kb on + strand, within GFF265 at 263.337 kb on - strand, within GFF265 at 263.337 kb on - strand, within GFF265 at 263.337 kb on - strand, within GFF265 at 263.372 kb on - strand at 263.389 kb on + strand at 263.394 kb on + strand at 263.399 kb on + strand at 263.422 kb on - strand at 263.424 kb on + strand at 263.446 kb on + strand at 263.470 kb on + strand at 263.470 kb on + strand at 263.478 kb on - strand, within GFF266 at 263.478 kb on - strand, within GFF266 at 263.478 kb on - strand, within GFF266 at 263.485 kb on - strand, within GFF266 at 263.509 kb on - strand, within GFF266 at 263.525 kb on - strand, within GFF266 at 263.562 kb on - strand, within GFF266
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with Sucrose and Ammonium Sulfate remove 261,387 + GFF260 0.68 +0.6 261,393 + GFF260 0.71 +0.5 261,393 + GFF260 0.71 -1.4 261,395 - GFF260 0.72 +0.5 261,398 + GFF260 0.74 -1.7 261,401 - GFF260 0.75 +0.1 261,411 - GFF260 0.81 -0.5 261,431 + +0.5 261,439 - +0.5 261,439 - +0.6 261,443 + -1.0 261,443 + -0.1 261,443 + +0.4 261,451 - -1.0 261,451 - +0.5 261,451 - -0.9 261,451 - +0.0 261,451 - +1.0 261,451 - -2.4 261,468 + GFF261 0.11 -0.8 261,468 + GFF261 0.11 +0.6 261,487 + GFF261 0.20 -0.5 261,487 + GFF261 0.20 +0.2 261,497 - GFF261 0.24 +0.2 261,497 - GFF261 0.24 -1.2 261,519 + GFF261 0.35 -0.2 261,519 + GFF261 0.35 -3.0 261,519 + GFF261 0.35 -0.9 261,527 - GFF261 0.38 -0.3 261,532 - GFF261 0.41 -0.7 261,542 + GFF261 0.46 -2.4 261,545 - GFF261 0.47 -0.0 261,552 + GFF261 0.50 +1.1 261,555 - GFF261 0.52 +0.2 261,559 + GFF261 0.54 -0.5 261,559 + GFF261 0.54 -0.6 261,559 + GFF261 0.54 +0.8 261,560 - GFF261 0.54 -1.6 261,576 + GFF261 0.62 -0.2 261,583 + GFF261 0.65 +0.2 261,583 + GFF261 0.65 +2.3 261,583 + GFF261 0.65 -0.5 261,591 - GFF261 0.69 +0.4 261,591 - GFF261 0.69 -0.3 261,599 + GFF261 0.72 -2.0 261,603 + GFF261 0.74 -2.2 261,603 + GFF261 0.74 -0.5 261,603 + GFF261 0.74 +1.3 261,603 + GFF261 0.74 +0.0 261,658 - +1.7 261,668 - +0.0 261,678 - +0.8 261,678 - +1.5 261,720 + GFF262 0.22 -0.4 261,724 + GFF262 0.23 +1.3 261,724 + GFF262 0.23 -0.4 261,731 - GFF262 0.25 +0.2 261,739 + GFF262 0.28 -0.2 261,742 - GFF262 0.29 +0.6 261,767 + GFF262 0.37 -0.2 261,796 + GFF262 0.47 +1.8 261,798 + GFF262 0.48 -0.7 261,824 + GFF262 0.56 +1.6 261,824 + GFF262 0.56 +0.3 261,829 + GFF262 0.58 +0.3 261,829 + GFF262 0.58 +1.8 261,832 - GFF262 0.59 -1.6 261,832 - GFF262 0.59 -1.6 261,832 - GFF262 0.59 -0.4 261,832 - GFF262 0.59 +0.3 261,832 - GFF262 0.59 -0.2 261,848 + GFF262 0.64 +2.5 261,911 - GFF262 0.85 +0.1 261,914 - GFF262 0.86 -0.5 261,928 - -0.1 261,945 - -1.2 261,950 + -2.3 261,950 + -0.6 261,958 - -0.8 261,958 - +0.0 261,958 - -0.5 261,958 - -1.6 261,960 - -0.1 261,966 - +0.8 261,966 - +0.3 261,966 - +0.9 261,986 + +1.0 261,986 + +0.9 261,996 + -0.7 262,003 + -0.4 262,003 + -0.9 262,011 - -0.2 262,049 - +0.5 262,066 + -1.2 262,073 + GFF263 0.11 +0.8 262,073 + GFF263 0.11 -0.8 262,074 - GFF263 0.11 -0.5 262,078 + GFF263 0.12 +0.1 262,081 - GFF263 0.13 +1.1 262,086 - GFF263 0.15 -0.9 262,086 - GFF263 0.15 +2.1 262,086 - GFF263 0.15 +0.8 262,088 + GFF263 0.15 -0.2 262,132 - GFF263 0.29 +1.3 262,134 + GFF263 0.29 -0.5 262,158 + GFF263 0.36 -0.6 262,158 + GFF263 0.36 -0.9 262,158 + GFF263 0.36 -1.1 262,166 - GFF263 0.39 +0.6 262,166 - GFF263 0.39 -0.4 262,166 - GFF263 0.39 +0.1 262,213 + GFF263 0.53 +0.3 262,233 - GFF263 0.59 -1.2 262,244 - GFF263 0.62 +0.9 262,250 + GFF263 0.64 -1.1 262,254 + GFF263 0.65 +1.2 262,336 - GFF263 0.89 +0.3 262,369 - -0.2 262,398 + GFF264 0.15 +0.2 262,398 - GFF264 0.15 -0.6 262,405 + GFF264 0.19 -1.9 262,405 + GFF264 0.19 -0.6 262,406 + GFF264 0.19 +0.2 262,414 - GFF264 0.23 -0.2 262,414 - GFF264 0.23 +0.2 262,414 - GFF264 0.23 -0.7 262,416 - GFF264 0.24 +1.2 262,416 - GFF264 0.24 +0.5 262,416 - GFF264 0.24 -1.4 262,416 - GFF264 0.24 +0.8 262,416 - GFF264 0.24 -0.7 262,436 + GFF264 0.34 +0.6 262,441 + GFF264 0.37 -0.2 262,441 + GFF264 0.37 +0.0 262,448 + GFF264 0.40 -1.0 262,448 + GFF264 0.40 -0.8 262,448 + GFF264 0.40 -1.4 262,449 - GFF264 0.41 -1.1 262,463 - GFF264 0.48 -1.2 262,465 + GFF264 0.49 +1.7 262,465 + GFF264 0.49 -0.5 262,465 + GFF264 0.49 -0.5 262,473 - GFF264 0.53 -0.1 262,473 - GFF264 0.53 +0.2 262,473 - GFF264 0.53 +1.5 262,480 + GFF264 0.57 +1.7 262,519 + GFF264 0.76 +1.3 262,535 + GFF264 0.84 +1.3 262,542 - GFF264 0.88 -1.9 262,543 - GFF264 0.88 -1.4 262,544 + GFF264 0.89 +0.2 262,544 + GFF264 0.89 +1.1 262,550 + -1.7 262,550 + -1.4 262,552 - +0.5 262,559 + +0.5 262,559 + +0.6 262,569 + -0.5 262,583 + +0.3 262,591 - -0.8 262,599 + +0.7 262,636 + +1.9 262,640 + -0.6 262,649 - -1.7 262,651 + -0.8 262,659 + +0.4 262,659 + +0.0 262,659 + +1.3 262,659 + +0.6 262,667 - +0.4 262,667 - +1.5 262,667 - +0.1 262,667 - -0.1 262,667 - -2.2 262,668 - -0.8 262,681 + +0.5 262,683 + -0.5 262,683 + +2.8 262,689 - -1.2 262,704 + -0.9 262,704 - -0.7 262,712 - +1.1 262,753 + GFF265 0.10 +1.1 262,755 + GFF265 0.10 +1.0 262,763 - GFF265 0.11 -0.4 262,867 - GFF265 0.25 +0.8 262,890 - GFF265 0.28 +2.1 262,890 - GFF265 0.28 +0.2 262,909 + GFF265 0.31 +1.3 262,910 - GFF265 0.31 +0.2 262,968 + GFF265 0.39 -0.3 262,976 + GFF265 0.40 -2.0 262,994 - GFF265 0.42 +0.2 263,022 + GFF265 0.46 -0.0 263,023 + GFF265 0.46 +0.2 263,053 + GFF265 0.50 -0.7 263,098 + GFF265 0.56 +0.3 263,118 + GFF265 0.59 +0.3 263,131 + GFF265 0.60 +0.2 263,161 + GFF265 0.64 +0.5 263,161 + GFF265 0.64 +0.3 263,161 + GFF265 0.64 -1.0 263,169 - GFF265 0.65 +0.5 263,186 - GFF265 0.68 -0.5 263,186 - GFF265 0.68 -0.8 263,186 - GFF265 0.68 +0.7 263,294 + GFF265 0.82 -0.9 263,294 + GFF265 0.82 -0.2 263,323 - GFF265 0.86 +0.1 263,324 + GFF265 0.86 -2.7 263,329 + GFF265 0.87 +2.2 263,337 - GFF265 0.88 -1.0 263,337 - GFF265 0.88 -0.1 263,337 - GFF265 0.88 -2.8 263,372 - +1.2 263,389 + +0.7 263,394 + -2.0 263,399 + -0.3 263,422 - -1.4 263,424 + +0.3 263,446 + +0.0 263,470 + +1.8 263,470 + +0.3 263,478 - GFF266 0.12 -0.2 263,478 - GFF266 0.12 +0.0 263,478 - GFF266 0.12 -1.2 263,485 - GFF266 0.15 +0.2 263,509 - GFF266 0.25 +0.1 263,525 - GFF266 0.32 -0.1 263,562 - GFF266 0.48 -1.2
Or see this region's nucleotide sequence