Experiment: EMM_noCarbon with Sucrose and Ammonium Sulfate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF222 and GFF223 are separated by 180 nucleotides GFF223 and GFF224 are separated by 154 nucleotides GFF224 and GFF225 are separated by 75 nucleotides GFF225 and GFF226 overlap by 39 nucleotides
GFF222 - hypothetical protein, at 227,788 to 228,771
GFF222
GFF223 - LSU ribosomal protein L28p @ LSU ribosomal protein L28p, zinc-independent, at 228,952 to 229,245
GFF223
GFF224 - hypothetical protein, at 229,400 to 229,621
GFF224
GFF225 - tRNA-specific adenosine-34 deaminase (EC 3.5.4.33), at 229,697 to 230,155
GFF225
GFF226 - hypothetical protein, at 230,117 to 232,030
GFF226
Position (kb) 229
230 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 228.404 kb on + strand, within GFF222 at 228.404 kb on + strand, within GFF222 at 228.431 kb on + strand, within GFF222 at 228.450 kb on + strand, within GFF222 at 228.458 kb on - strand, within GFF222 at 228.458 kb on - strand, within GFF222 at 228.458 kb on - strand, within GFF222 at 228.470 kb on + strand, within GFF222 at 228.470 kb on + strand, within GFF222 at 228.470 kb on + strand, within GFF222 at 228.471 kb on - strand, within GFF222 at 228.476 kb on - strand, within GFF222 at 228.496 kb on - strand, within GFF222 at 228.501 kb on - strand, within GFF222 at 228.530 kb on + strand, within GFF222 at 228.547 kb on - strand, within GFF222 at 228.547 kb on - strand, within GFF222 at 228.549 kb on + strand, within GFF222 at 228.599 kb on + strand, within GFF222 at 228.607 kb on - strand, within GFF222 at 228.607 kb on - strand, within GFF222 at 228.672 kb on - strand, within GFF222 at 228.673 kb on - strand at 228.677 kb on + strand at 228.677 kb on + strand at 228.677 kb on + strand at 228.677 kb on + strand at 228.677 kb on + strand at 228.677 kb on + strand at 228.677 kb on + strand at 228.677 kb on + strand at 228.677 kb on + strand at 228.677 kb on + strand at 228.685 kb on - strand at 228.685 kb on - strand at 228.685 kb on - strand at 228.685 kb on - strand at 228.687 kb on + strand at 228.687 kb on + strand at 228.718 kb on - strand at 228.765 kb on + strand at 228.773 kb on - strand at 228.777 kb on + strand at 228.783 kb on - strand at 228.803 kb on - strand at 228.803 kb on - strand at 228.814 kb on - strand at 228.846 kb on - strand at 229.311 kb on + strand at 229.329 kb on + strand at 229.370 kb on + strand at 229.378 kb on + strand at 229.378 kb on - strand at 229.380 kb on + strand at 229.383 kb on + strand at 229.385 kb on + strand at 229.388 kb on + strand at 229.391 kb on - strand at 229.398 kb on - strand at 229.477 kb on + strand, within GFF224 at 229.540 kb on - strand, within GFF224 at 229.679 kb on + strand at 229.681 kb on + strand at 230.158 kb on + strand at 230.163 kb on + strand at 230.163 kb on + strand at 230.163 kb on + strand at 230.163 kb on + strand at 230.163 kb on + strand at 230.171 kb on - strand at 230.175 kb on + strand at 230.175 kb on + strand at 230.175 kb on + strand at 230.183 kb on - strand at 230.248 kb on - strand at 230.355 kb on - strand, within GFF226
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with Sucrose and Ammonium Sulfate remove 228,404 + GFF222 0.63 -0.3 228,404 + GFF222 0.63 -1.6 228,431 + GFF222 0.65 +0.2 228,450 + GFF222 0.67 +0.9 228,458 - GFF222 0.68 -0.4 228,458 - GFF222 0.68 -0.8 228,458 - GFF222 0.68 -0.8 228,470 + GFF222 0.69 +0.1 228,470 + GFF222 0.69 +0.4 228,470 + GFF222 0.69 +2.0 228,471 - GFF222 0.69 +0.8 228,476 - GFF222 0.70 +0.0 228,496 - GFF222 0.72 -0.8 228,501 - GFF222 0.72 -0.0 228,530 + GFF222 0.75 +1.6 228,547 - GFF222 0.77 +0.3 228,547 - GFF222 0.77 +0.2 228,549 + GFF222 0.77 -0.2 228,599 + GFF222 0.82 -0.2 228,607 - GFF222 0.83 -0.5 228,607 - GFF222 0.83 +1.0 228,672 - GFF222 0.90 -1.0 228,673 - +0.1 228,677 + -0.4 228,677 + -1.1 228,677 + +0.5 228,677 + +0.5 228,677 + -0.3 228,677 + -0.4 228,677 + +0.2 228,677 + -0.6 228,677 + -0.9 228,677 + -1.1 228,685 - -0.1 228,685 - -0.7 228,685 - -1.2 228,685 - +0.1 228,687 + +0.1 228,687 + +0.6 228,718 - +0.8 228,765 + +1.0 228,773 - +0.6 228,777 + +0.8 228,783 - +0.5 228,803 - -0.8 228,803 - +1.0 228,814 - -0.2 228,846 - -1.7 229,311 + -1.0 229,329 + -0.0 229,370 + -0.6 229,378 + -0.6 229,378 - +0.8 229,380 + -0.0 229,383 + -0.2 229,385 + +1.4 229,388 + -0.2 229,391 - +1.4 229,398 - -2.9 229,477 + GFF224 0.35 -0.3 229,540 - GFF224 0.63 +0.4 229,679 + +0.5 229,681 + +0.3 230,158 + +1.0 230,163 + -0.3 230,163 + +0.2 230,163 + -0.9 230,163 + -0.8 230,163 + +2.1 230,171 - -2.0 230,175 + -0.4 230,175 + -0.2 230,175 + -0.8 230,183 - +0.4 230,248 - -0.9 230,355 - GFF226 0.12 +0.4
Or see this region's nucleotide sequence