Strain Fitness in Sphingobium sp. HT1-2 around GFF221

Experiment: EMM_noCarbon with Sucrose and Ammonium Sulfate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF218 and GFF219 are separated by 213 nucleotidesGFF219 and GFF220 are separated by 83 nucleotidesGFF220 and GFF221 are separated by 56 nucleotidesGFF221 and GFF222 are separated by 183 nucleotides GFF218 - Transcriptional regulator, ArsR family / Methyltransferase fusion, at 225,402 to 226,373 GFF218 GFF219 - hypothetical protein, at 226,587 to 226,805 GFF219 GFF220 - hypothetical protein, at 226,889 to 227,122 GFF220 GFF221 - hypothetical protein, at 227,179 to 227,604 GFF221 GFF222 - hypothetical protein, at 227,788 to 228,771 GFF222 Position (kb) 227 228Strain fitness (log2 ratio) -2 -1 0 1 2at 226.249 kb on + strand, within GFF218at 226.269 kb on - strand, within GFF218at 226.300 kb on + strandat 226.308 kb on - strandat 226.353 kb on - strandat 226.450 kb on + strandat 226.458 kb on - strandat 226.488 kb on - strandat 226.488 kb on - strandat 226.488 kb on - strandat 226.488 kb on - strandat 226.488 kb on - strandat 226.488 kb on - strandat 226.488 kb on - strandat 226.488 kb on - strandat 226.490 kb on + strandat 226.490 kb on + strandat 226.498 kb on - strandat 226.639 kb on - strand, within GFF219at 226.700 kb on - strand, within GFF219at 226.748 kb on + strand, within GFF219at 226.976 kb on + strand, within GFF220at 226.976 kb on + strand, within GFF220at 226.976 kb on + strand, within GFF220at 226.976 kb on + strand, within GFF220at 226.976 kb on + strand, within GFF220at 227.060 kb on - strand, within GFF220at 227.061 kb on - strand, within GFF220at 227.170 kb on + strandat 227.270 kb on + strand, within GFF221at 227.275 kb on + strand, within GFF221at 227.283 kb on - strand, within GFF221at 227.283 kb on - strand, within GFF221at 227.283 kb on - strand, within GFF221at 227.301 kb on + strand, within GFF221at 227.334 kb on - strand, within GFF221at 227.343 kb on + strand, within GFF221at 227.351 kb on - strand, within GFF221at 227.437 kb on - strand, within GFF221at 227.503 kb on + strand, within GFF221at 227.503 kb on + strand, within GFF221at 227.503 kb on + strand, within GFF221at 227.511 kb on - strand, within GFF221at 227.549 kb on - strand, within GFF221at 227.606 kb on + strandat 227.669 kb on + strandat 227.669 kb on + strandat 227.718 kb on + strandat 227.726 kb on - strandat 227.726 kb on - strandat 227.726 kb on - strandat 227.745 kb on + strandat 227.745 kb on + strandat 227.745 kb on + strandat 227.750 kb on + strandat 227.753 kb on - strandat 227.753 kb on - strandat 227.753 kb on - strandat 227.753 kb on - strandat 227.792 kb on + strandat 227.800 kb on - strandat 227.800 kb on - strandat 227.834 kb on - strandat 227.847 kb on + strandat 227.868 kb on + strandat 227.868 kb on + strandat 227.868 kb on + strandat 227.880 kb on + strandat 227.883 kb on + strandat 227.905 kb on + strand, within GFF222at 228.020 kb on - strand, within GFF222at 228.025 kb on + strand, within GFF222at 228.093 kb on + strand, within GFF222at 228.127 kb on + strand, within GFF222at 228.132 kb on + strand, within GFF222at 228.151 kb on - strand, within GFF222at 228.176 kb on + strand, within GFF222at 228.209 kb on + strand, within GFF222at 228.209 kb on + strand, within GFF222at 228.209 kb on + strand, within GFF222at 228.209 kb on + strand, within GFF222at 228.209 kb on + strand, within GFF222at 228.210 kb on + strand, within GFF222at 228.217 kb on - strand, within GFF222at 228.217 kb on - strand, within GFF222at 228.219 kb on + strand, within GFF222at 228.258 kb on + strand, within GFF222at 228.258 kb on + strand, within GFF222at 228.266 kb on - strand, within GFF222at 228.276 kb on - strand, within GFF222at 228.297 kb on + strand, within GFF222at 228.297 kb on + strand, within GFF222at 228.305 kb on - strand, within GFF222at 228.310 kb on - strand, within GFF222at 228.366 kb on + strand, within GFF222at 228.366 kb on + strand, within GFF222at 228.366 kb on + strand, within GFF222at 228.374 kb on - strand, within GFF222at 228.404 kb on + strand, within GFF222at 228.404 kb on + strand, within GFF222at 228.431 kb on + strand, within GFF222at 228.450 kb on + strand, within GFF222at 228.458 kb on - strand, within GFF222at 228.458 kb on - strand, within GFF222at 228.458 kb on - strand, within GFF222at 228.470 kb on + strand, within GFF222at 228.470 kb on + strand, within GFF222at 228.470 kb on + strand, within GFF222at 228.471 kb on - strand, within GFF222at 228.476 kb on - strand, within GFF222at 228.496 kb on - strand, within GFF222at 228.501 kb on - strand, within GFF222at 228.530 kb on + strand, within GFF222at 228.547 kb on - strand, within GFF222at 228.547 kb on - strand, within GFF222at 228.549 kb on + strand, within GFF222at 228.599 kb on + strand, within GFF222

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Per-strain Table

Position Strand Gene LocusTag Fraction EMM_noCarbon with Sucrose and Ammonium Sulfate
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226,249 + GFF218 0.87 -1.6
226,269 - GFF218 0.89 -0.2
226,300 + -2.3
226,308 - +0.8
226,353 - +0.5
226,450 + +1.0
226,458 - +0.1
226,488 - -0.4
226,488 - +0.4
226,488 - +1.0
226,488 - -0.5
226,488 - +1.9
226,488 - +0.4
226,488 - +0.8
226,488 - +0.2
226,490 + +1.4
226,490 + +0.1
226,498 - +1.0
226,639 - GFF219 0.24 -1.1
226,700 - GFF219 0.52 -0.7
226,748 + GFF219 0.74 +0.7
226,976 + GFF220 0.37 -1.5
226,976 + GFF220 0.37 -0.4
226,976 + GFF220 0.37 -1.1
226,976 + GFF220 0.37 -1.5
226,976 + GFF220 0.37 -0.5
227,060 - GFF220 0.73 -0.4
227,061 - GFF220 0.74 -0.7
227,170 + +1.7
227,270 + GFF221 0.21 -0.5
227,275 + GFF221 0.23 +0.2
227,283 - GFF221 0.24 -1.4
227,283 - GFF221 0.24 +0.7
227,283 - GFF221 0.24 +1.1
227,301 + GFF221 0.29 -0.1
227,334 - GFF221 0.36 +1.1
227,343 + GFF221 0.38 +0.1
227,351 - GFF221 0.40 +0.6
227,437 - GFF221 0.61 -1.3
227,503 + GFF221 0.76 -1.2
227,503 + GFF221 0.76 -0.2
227,503 + GFF221 0.76 +0.3
227,511 - GFF221 0.78 +0.0
227,549 - GFF221 0.87 -0.6
227,606 + -1.2
227,669 + -1.5
227,669 + +0.0
227,718 + +0.1
227,726 - +0.6
227,726 - -1.3
227,726 - -1.3
227,745 + +1.3
227,745 + -0.3
227,745 + +1.0
227,750 + +1.3
227,753 - -0.6
227,753 - -0.1
227,753 - -0.0
227,753 - -1.2
227,792 + -1.4
227,800 - -0.3
227,800 - -0.2
227,834 - -1.3
227,847 + +0.2
227,868 + +0.2
227,868 + +1.5
227,868 + -2.2
227,880 + +0.6
227,883 + +0.1
227,905 + GFF222 0.12 -1.9
228,020 - GFF222 0.24 +0.6
228,025 + GFF222 0.24 +0.1
228,093 + GFF222 0.31 -0.2
228,127 + GFF222 0.34 -1.4
228,132 + GFF222 0.35 +0.1
228,151 - GFF222 0.37 -1.7
228,176 + GFF222 0.39 -1.5
228,209 + GFF222 0.43 -0.3
228,209 + GFF222 0.43 +1.4
228,209 + GFF222 0.43 -0.2
228,209 + GFF222 0.43 +1.0
228,209 + GFF222 0.43 -0.2
228,210 + GFF222 0.43 -0.7
228,217 - GFF222 0.44 +1.2
228,217 - GFF222 0.44 +0.4
228,219 + GFF222 0.44 -0.4
228,258 + GFF222 0.48 +0.4
228,258 + GFF222 0.48 +0.8
228,266 - GFF222 0.49 +0.2
228,276 - GFF222 0.50 +1.6
228,297 + GFF222 0.52 -0.7
228,297 + GFF222 0.52 +0.3
228,305 - GFF222 0.53 +0.2
228,310 - GFF222 0.53 -0.4
228,366 + GFF222 0.59 +0.6
228,366 + GFF222 0.59 +0.3
228,366 + GFF222 0.59 -0.4
228,374 - GFF222 0.60 -1.0
228,404 + GFF222 0.63 -0.3
228,404 + GFF222 0.63 -1.6
228,431 + GFF222 0.65 +0.2
228,450 + GFF222 0.67 +0.9
228,458 - GFF222 0.68 -0.4
228,458 - GFF222 0.68 -0.8
228,458 - GFF222 0.68 -0.8
228,470 + GFF222 0.69 +0.1
228,470 + GFF222 0.69 +0.4
228,470 + GFF222 0.69 +2.0
228,471 - GFF222 0.69 +0.8
228,476 - GFF222 0.70 +0.0
228,496 - GFF222 0.72 -0.8
228,501 - GFF222 0.72 -0.0
228,530 + GFF222 0.75 +1.6
228,547 - GFF222 0.77 +0.3
228,547 - GFF222 0.77 +0.2
228,549 + GFF222 0.77 -0.2
228,599 + GFF222 0.82 -0.2

Or see this region's nucleotide sequence