Experiment: EMM_noCarbon with Sucrose and Ammonium Sulfate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1449 and GFF1450 are separated by 88 nucleotides GFF1450 and GFF1451 overlap by 4 nucleotides GFF1451 and GFF1452 are separated by 1 nucleotides
GFF1449 - Succinylglutamic semialdehyde dehydrogenase (EC 1.2.1.71), at 1,476,420 to 1,477,853
GFF1449
GFF1450 - Uroporphyrinogen-III methyltransferase (EC 2.1.1.107), at 1,477,942 to 1,478,730
GFF1450
GFF1451 - Conserved hypothetical protein probably involved in sulfate reduction, at 1,478,727 to 1,479,023
GFF1451
GFF1452 - Sulfite reductase [NADPH] hemoprotein beta-component (EC 1.8.1.2), at 1,479,025 to 1,480,656
GFF1452
Position (kb)
1478
1479
1480 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1477.730 kb on + strand at 1477.733 kb on - strand at 1477.738 kb on - strand at 1477.767 kb on - strand at 1477.783 kb on + strand at 1477.793 kb on + strand at 1477.796 kb on - strand at 1477.801 kb on - strand at 1477.824 kb on - strand at 1477.824 kb on - strand at 1477.824 kb on - strand at 1477.826 kb on - strand at 1477.840 kb on - strand at 1477.875 kb on - strand at 1477.888 kb on - strand at 1477.898 kb on - strand at 1477.933 kb on + strand at 1477.958 kb on - strand at 1478.656 kb on + strand at 1478.656 kb on + strand at 1478.657 kb on + strand at 1478.664 kb on - strand at 1478.685 kb on - strand at 1478.950 kb on + strand, within GFF1451 at 1478.950 kb on + strand, within GFF1451 at 1478.950 kb on + strand, within GFF1451 at 1478.958 kb on - strand, within GFF1451 at 1478.968 kb on + strand, within GFF1451 at 1478.973 kb on + strand, within GFF1451 at 1478.976 kb on - strand, within GFF1451 at 1479.003 kb on + strand at 1479.025 kb on - strand at 1479.041 kb on + strand at 1479.049 kb on - strand at 1479.113 kb on + strand at 1479.176 kb on + strand at 1479.176 kb on + strand at 1479.184 kb on - strand at 1479.378 kb on - strand, within GFF1452 at 1479.412 kb on - strand, within GFF1452
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with Sucrose and Ammonium Sulfate remove 1,477,730 + +0.1 1,477,733 - -0.8 1,477,738 - -1.0 1,477,767 - +0.7 1,477,783 + +0.1 1,477,793 + -2.1 1,477,796 - +0.1 1,477,801 - +0.1 1,477,824 - +0.1 1,477,824 - -1.5 1,477,824 - +0.9 1,477,826 - +0.4 1,477,840 - -1.2 1,477,875 - +1.1 1,477,888 - -2.3 1,477,898 - -1.9 1,477,933 + +1.8 1,477,958 - -0.6 1,478,656 + -0.4 1,478,656 + -0.2 1,478,657 + -0.3 1,478,664 - +0.1 1,478,685 - -1.3 1,478,950 + GFF1451 0.75 -1.4 1,478,950 + GFF1451 0.75 -1.3 1,478,950 + GFF1451 0.75 -2.3 1,478,958 - GFF1451 0.78 +0.7 1,478,968 + GFF1451 0.81 -0.3 1,478,973 + GFF1451 0.83 -0.1 1,478,976 - GFF1451 0.84 -0.7 1,479,003 + +0.9 1,479,025 - -1.2 1,479,041 + -1.7 1,479,049 - -1.3 1,479,113 + -1.2 1,479,176 + -2.1 1,479,176 + -1.2 1,479,184 - -1.6 1,479,378 - GFF1452 0.22 +0.3 1,479,412 - GFF1452 0.24 -1.7
Or see this region's nucleotide sequence