Experiment: EMM_noCarbon with Sucrose and Ammonium Sulfate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1 and GFF2 are separated by 66 nucleotides
GFF1 - hypothetical protein, at 285 to 1,115
GFF1
GFF2 - hypothetical protein, at 1,182 to 1,940
GFF2
Position (kb) 0
1
2 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 0.134 kb on + strand at 0.134 kb on + strand at 0.134 kb on + strand at 0.134 kb on + strand at 0.142 kb on + strand at 0.142 kb on - strand at 0.142 kb on - strand at 0.142 kb on - strand at 0.144 kb on - strand at 0.155 kb on + strand at 0.162 kb on + strand at 0.162 kb on + strand at 0.162 kb on + strand at 0.162 kb on + strand at 0.170 kb on - strand at 0.215 kb on - strand at 0.219 kb on + strand at 0.219 kb on + strand at 0.225 kb on + strand at 0.225 kb on - strand at 0.225 kb on - strand at 0.225 kb on - strand at 0.225 kb on - strand at 0.299 kb on + strand at 0.299 kb on + strand at 0.299 kb on + strand at 0.299 kb on + strand at 0.299 kb on + strand at 0.299 kb on + strand at 0.299 kb on + strand at 0.301 kb on + strand at 0.301 kb on + strand at 0.307 kb on - strand at 0.307 kb on - strand at 0.307 kb on - strand at 0.309 kb on - strand at 0.336 kb on + strand at 0.338 kb on + strand at 0.339 kb on - strand at 0.356 kb on - strand at 0.358 kb on + strand at 0.363 kb on + strand at 0.366 kb on - strand at 0.379 kb on + strand, within GFF1 at 0.397 kb on + strand, within GFF1 at 0.409 kb on - strand, within GFF1 at 0.468 kb on + strand, within GFF1 at 0.468 kb on + strand, within GFF1 at 0.468 kb on + strand, within GFF1 at 0.468 kb on + strand, within GFF1 at 0.468 kb on + strand, within GFF1 at 0.469 kb on + strand, within GFF1 at 0.470 kb on + strand, within GFF1 at 0.470 kb on - strand, within GFF1 at 0.476 kb on - strand, within GFF1 at 0.476 kb on - strand, within GFF1 at 0.477 kb on - strand, within GFF1 at 0.477 kb on - strand, within GFF1 at 0.480 kb on + strand, within GFF1 at 0.488 kb on - strand, within GFF1 at 0.519 kb on + strand, within GFF1 at 0.527 kb on - strand, within GFF1 at 0.527 kb on - strand, within GFF1 at 0.527 kb on - strand, within GFF1 at 0.527 kb on - strand, within GFF1 at 0.536 kb on + strand, within GFF1 at 0.627 kb on - strand, within GFF1 at 0.628 kb on + strand, within GFF1 at 0.628 kb on + strand, within GFF1 at 0.628 kb on + strand, within GFF1 at 0.656 kb on - strand, within GFF1 at 0.663 kb on + strand, within GFF1 at 0.663 kb on + strand, within GFF1 at 0.663 kb on + strand, within GFF1 at 0.663 kb on + strand, within GFF1 at 0.663 kb on + strand, within GFF1 at 0.671 kb on - strand, within GFF1 at 0.671 kb on - strand, within GFF1 at 0.671 kb on - strand, within GFF1 at 0.671 kb on - strand, within GFF1 at 0.697 kb on + strand, within GFF1 at 0.697 kb on + strand, within GFF1 at 0.697 kb on + strand, within GFF1 at 0.705 kb on - strand, within GFF1 at 0.705 kb on - strand, within GFF1 at 0.717 kb on - strand, within GFF1 at 0.719 kb on - strand, within GFF1 at 0.741 kb on + strand, within GFF1 at 0.743 kb on + strand, within GFF1 at 0.743 kb on + strand, within GFF1 at 0.751 kb on - strand, within GFF1 at 0.751 kb on - strand, within GFF1 at 0.751 kb on - strand, within GFF1 at 0.751 kb on - strand, within GFF1 at 0.751 kb on - strand, within GFF1 at 0.760 kb on - strand, within GFF1 at 0.767 kb on - strand, within GFF1 at 0.772 kb on + strand, within GFF1 at 0.772 kb on + strand, within GFF1 at 0.772 kb on + strand, within GFF1 at 0.780 kb on - strand, within GFF1 at 0.791 kb on - strand, within GFF1 at 0.808 kb on + strand, within GFF1 at 0.808 kb on + strand, within GFF1 at 0.808 kb on + strand, within GFF1 at 0.816 kb on - strand, within GFF1 at 0.833 kb on + strand, within GFF1 at 0.833 kb on + strand, within GFF1 at 0.833 kb on + strand, within GFF1 at 0.841 kb on - strand, within GFF1 at 0.857 kb on - strand, within GFF1 at 0.870 kb on - strand, within GFF1 at 0.870 kb on - strand, within GFF1 at 0.882 kb on + strand, within GFF1 at 0.882 kb on - strand, within GFF1 at 0.887 kb on + strand, within GFF1 at 0.890 kb on - strand, within GFF1 at 0.895 kb on - strand, within GFF1 at 0.895 kb on - strand, within GFF1 at 0.931 kb on + strand, within GFF1 at 0.931 kb on + strand, within GFF1 at 0.939 kb on - strand, within GFF1 at 0.947 kb on - strand, within GFF1 at 0.956 kb on + strand, within GFF1 at 0.956 kb on + strand, within GFF1 at 0.964 kb on - strand, within GFF1 at 0.964 kb on - strand, within GFF1 at 0.964 kb on - strand, within GFF1 at 0.964 kb on - strand, within GFF1 at 0.964 kb on - strand, within GFF1 at 0.966 kb on - strand, within GFF1 at 1.003 kb on - strand, within GFF1 at 1.055 kb on + strand at 1.066 kb on + strand at 1.081 kb on - strand at 1.126 kb on + strand at 1.126 kb on + strand at 1.134 kb on - strand at 1.144 kb on + strand at 1.152 kb on + strand at 1.152 kb on + strand at 1.152 kb on - strand at 1.152 kb on - strand at 1.157 kb on + strand at 1.162 kb on + strand at 1.165 kb on - strand at 1.170 kb on - strand at 1.170 kb on - strand at 1.192 kb on + strand at 1.202 kb on + strand at 1.210 kb on - strand at 1.249 kb on - strand at 1.249 kb on - strand at 1.250 kb on - strand at 1.258 kb on - strand, within GFF2 at 1.285 kb on + strand, within GFF2 at 1.285 kb on + strand, within GFF2 at 1.285 kb on + strand, within GFF2 at 1.293 kb on + strand, within GFF2 at 1.293 kb on - strand, within GFF2 at 1.293 kb on - strand, within GFF2 at 1.299 kb on - strand, within GFF2 at 1.299 kb on - strand, within GFF2 at 1.341 kb on + strand, within GFF2 at 1.359 kb on + strand, within GFF2 at 1.359 kb on + strand, within GFF2 at 1.359 kb on + strand, within GFF2 at 1.359 kb on + strand, within GFF2 at 1.359 kb on + strand, within GFF2 at 1.359 kb on + strand, within GFF2 at 1.367 kb on - strand, within GFF2 at 1.367 kb on - strand, within GFF2 at 1.367 kb on - strand, within GFF2 at 1.367 kb on - strand, within GFF2 at 1.367 kb on - strand, within GFF2 at 1.367 kb on - strand, within GFF2 at 1.368 kb on + strand, within GFF2 at 1.373 kb on + strand, within GFF2 at 1.396 kb on + strand, within GFF2 at 1.404 kb on - strand, within GFF2 at 1.406 kb on + strand, within GFF2 at 1.406 kb on + strand, within GFF2 at 1.406 kb on + strand, within GFF2 at 1.412 kb on - strand, within GFF2 at 1.414 kb on - strand, within GFF2 at 1.414 kb on - strand, within GFF2 at 1.414 kb on - strand, within GFF2 at 1.414 kb on - strand, within GFF2 at 1.427 kb on - strand, within GFF2 at 1.438 kb on - strand, within GFF2 at 1.440 kb on - strand, within GFF2 at 1.469 kb on - strand, within GFF2 at 1.469 kb on - strand, within GFF2 at 1.469 kb on - strand, within GFF2 at 1.495 kb on - strand, within GFF2 at 1.511 kb on + strand, within GFF2 at 1.577 kb on + strand, within GFF2 at 1.598 kb on + strand, within GFF2 at 1.611 kb on - strand, within GFF2 at 1.628 kb on - strand, within GFF2 at 1.637 kb on - strand, within GFF2 at 1.639 kb on - strand, within GFF2 at 1.656 kb on + strand, within GFF2 at 1.656 kb on + strand, within GFF2 at 1.664 kb on - strand, within GFF2 at 1.743 kb on + strand, within GFF2 at 1.743 kb on + strand, within GFF2 at 1.748 kb on + strand, within GFF2 at 1.749 kb on + strand, within GFF2 at 1.751 kb on - strand, within GFF2 at 1.760 kb on + strand, within GFF2 at 1.766 kb on - strand, within GFF2 at 1.781 kb on + strand, within GFF2 at 1.786 kb on + strand, within GFF2 at 1.796 kb on + strand, within GFF2 at 1.845 kb on - strand, within GFF2 at 1.857 kb on + strand, within GFF2 at 1.896 kb on + strand at 2.045 kb on - strand at 2.063 kb on - strand at 2.071 kb on + strand at 2.071 kb on + strand at 2.071 kb on + strand at 2.071 kb on + strand at 2.071 kb on + strand at 2.071 kb on + strand at 2.071 kb on + strand at 2.071 kb on + strand at 2.071 kb on + strand at 2.071 kb on + strand at 2.071 kb on + strand at 2.071 kb on + strand at 2.071 kb on + strand at 2.071 kb on + strand at 2.071 kb on + strand at 2.071 kb on + strand at 2.079 kb on - strand at 2.079 kb on - strand at 2.079 kb on - strand at 2.079 kb on - strand at 2.079 kb on - strand at 2.079 kb on - strand at 2.079 kb on - strand at 2.079 kb on - strand at 2.079 kb on - strand at 2.079 kb on - strand at 2.079 kb on - strand at 2.079 kb on - strand at 2.079 kb on - strand at 2.079 kb on - strand at 2.079 kb on - strand at 2.079 kb on - strand at 2.079 kb on - strand at 2.079 kb on - strand at 2.079 kb on - strand at 2.079 kb on - strand at 2.079 kb on - strand at 2.079 kb on - strand at 2.079 kb on - strand at 2.079 kb on - strand at 2.079 kb on - strand at 2.079 kb on - strand at 2.079 kb on - strand at 2.079 kb on - strand at 2.079 kb on - strand at 2.079 kb on - strand at 2.079 kb on - strand at 2.079 kb on - strand at 2.079 kb on - strand at 2.079 kb on - strand at 2.079 kb on - strand at 2.079 kb on - strand at 2.081 kb on + strand at 2.081 kb on - strand at 2.088 kb on + strand at 2.113 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with Sucrose and Ammonium Sulfate remove 134 + -0.1 134 + -0.7 134 + +0.5 134 + +1.4 142 + +0.1 142 - -1.1 142 - +0.7 142 - -0.9 144 - +0.8 155 + +1.6 162 + -0.4 162 + +0.4 162 + -0.0 162 + -2.3 170 - +1.1 215 - -0.0 219 + -0.5 219 + -2.1 225 + -0.6 225 - +0.5 225 - +0.8 225 - +1.7 225 - -1.0 299 + -0.5 299 + -1.0 299 + -0.2 299 + -1.6 299 + -0.2 299 + -0.3 299 + +0.8 301 + +0.3 301 + -0.5 307 - +0.7 307 - +0.5 307 - -0.0 309 - +0.5 336 + +0.3 338 + +1.3 339 - +0.5 356 - +1.1 358 + -1.0 363 + +0.2 366 - -0.5 379 + GFF1 0.11 -0.7 397 + GFF1 0.13 -1.5 409 - GFF1 0.15 +0.6 468 + GFF1 0.22 -0.1 468 + GFF1 0.22 +0.3 468 + GFF1 0.22 -0.5 468 + GFF1 0.22 +1.0 468 + GFF1 0.22 -0.5 469 + GFF1 0.22 +0.5 470 + GFF1 0.22 +0.2 470 - GFF1 0.22 -2.8 476 - GFF1 0.23 +1.5 476 - GFF1 0.23 -0.2 477 - GFF1 0.23 -0.6 477 - GFF1 0.23 -1.2 480 + GFF1 0.23 -0.7 488 - GFF1 0.24 -0.4 519 + GFF1 0.28 +0.0 527 - GFF1 0.29 -0.1 527 - GFF1 0.29 +0.0 527 - GFF1 0.29 -1.9 527 - GFF1 0.29 -0.8 536 + GFF1 0.30 -0.3 627 - GFF1 0.41 +0.1 628 + GFF1 0.41 -0.6 628 + GFF1 0.41 +0.2 628 + GFF1 0.41 +0.7 656 - GFF1 0.45 -1.5 663 + GFF1 0.45 +0.9 663 + GFF1 0.45 +1.0 663 + GFF1 0.45 -0.6 663 + GFF1 0.45 +0.9 663 + GFF1 0.45 -0.4 671 - GFF1 0.46 -1.4 671 - GFF1 0.46 +1.4 671 - GFF1 0.46 +0.1 671 - GFF1 0.46 -0.2 697 + GFF1 0.50 -1.4 697 + GFF1 0.50 -0.7 697 + GFF1 0.50 -0.9 705 - GFF1 0.51 +0.4 705 - GFF1 0.51 +0.7 717 - GFF1 0.52 +0.6 719 - GFF1 0.52 +1.1 741 + GFF1 0.55 +0.4 743 + GFF1 0.55 +0.5 743 + GFF1 0.55 -0.5 751 - GFF1 0.56 +0.8 751 - GFF1 0.56 +0.3 751 - GFF1 0.56 +0.2 751 - GFF1 0.56 -0.1 751 - GFF1 0.56 -1.2 760 - GFF1 0.57 -0.2 767 - GFF1 0.58 -1.1 772 + GFF1 0.59 -1.4 772 + GFF1 0.59 -0.4 772 + GFF1 0.59 +0.1 780 - GFF1 0.60 -0.3 791 - GFF1 0.61 +1.1 808 + GFF1 0.63 -0.9 808 + GFF1 0.63 -2.2 808 + GFF1 0.63 -0.2 816 - GFF1 0.64 +1.1 833 + GFF1 0.66 +0.1 833 + GFF1 0.66 -1.1 833 + GFF1 0.66 +0.6 841 - GFF1 0.67 -0.2 857 - GFF1 0.69 -0.3 870 - GFF1 0.70 +0.1 870 - GFF1 0.70 +0.7 882 + GFF1 0.72 -0.0 882 - GFF1 0.72 +0.4 887 + GFF1 0.72 +0.8 890 - GFF1 0.73 -0.3 895 - GFF1 0.73 +0.4 895 - GFF1 0.73 +0.1 931 + GFF1 0.78 +0.0 931 + GFF1 0.78 +0.2 939 - GFF1 0.79 -1.3 947 - GFF1 0.80 -0.8 956 + GFF1 0.81 +0.5 956 + GFF1 0.81 -0.1 964 - GFF1 0.82 -1.2 964 - GFF1 0.82 -1.5 964 - GFF1 0.82 -0.7 964 - GFF1 0.82 +0.8 964 - GFF1 0.82 +0.2 966 - GFF1 0.82 -0.2 1,003 - GFF1 0.86 -1.5 1,055 + -0.7 1,066 + +0.3 1,081 - -0.6 1,126 + -0.1 1,126 + -0.3 1,134 - -0.3 1,144 + +1.1 1,152 + -1.7 1,152 + -0.2 1,152 - +0.1 1,152 - -0.1 1,157 + -1.3 1,162 + -0.5 1,165 - +0.3 1,170 - -1.2 1,170 - +0.4 1,192 + -0.0 1,202 + +0.5 1,210 - -1.1 1,249 - +0.9 1,249 - -0.2 1,250 - -1.6 1,258 - GFF2 0.10 +0.3 1,285 + GFF2 0.14 -1.4 1,285 + GFF2 0.14 +0.2 1,285 + GFF2 0.14 -0.6 1,293 + GFF2 0.15 -0.3 1,293 - GFF2 0.15 +1.1 1,293 - GFF2 0.15 +0.5 1,299 - GFF2 0.15 +0.4 1,299 - GFF2 0.15 +0.5 1,341 + GFF2 0.21 -0.4 1,359 + GFF2 0.23 -0.3 1,359 + GFF2 0.23 -1.4 1,359 + GFF2 0.23 +0.1 1,359 + GFF2 0.23 -1.8 1,359 + GFF2 0.23 +0.7 1,359 + GFF2 0.23 -1.0 1,367 - GFF2 0.24 +0.7 1,367 - GFF2 0.24 -0.6 1,367 - GFF2 0.24 +0.8 1,367 - GFF2 0.24 +0.5 1,367 - GFF2 0.24 -1.1 1,367 - GFF2 0.24 +0.8 1,368 + GFF2 0.25 -1.9 1,373 + GFF2 0.25 +0.1 1,396 + GFF2 0.28 -1.6 1,404 - GFF2 0.29 -1.0 1,406 + GFF2 0.30 -1.4 1,406 + GFF2 0.30 -0.8 1,406 + GFF2 0.30 -0.1 1,412 - GFF2 0.30 -0.4 1,414 - GFF2 0.31 +0.5 1,414 - GFF2 0.31 -0.5 1,414 - GFF2 0.31 -0.4 1,414 - GFF2 0.31 +0.5 1,427 - GFF2 0.32 +0.5 1,438 - GFF2 0.34 -0.2 1,440 - GFF2 0.34 -1.1 1,469 - GFF2 0.38 +0.8 1,469 - GFF2 0.38 +0.7 1,469 - GFF2 0.38 -0.5 1,495 - GFF2 0.41 -0.1 1,511 + GFF2 0.43 +0.3 1,577 + GFF2 0.52 -1.2 1,598 + GFF2 0.55 +0.6 1,611 - GFF2 0.57 +1.4 1,628 - GFF2 0.59 -0.6 1,637 - GFF2 0.60 +0.3 1,639 - GFF2 0.60 +0.4 1,656 + GFF2 0.62 -1.8 1,656 + GFF2 0.62 +1.3 1,664 - GFF2 0.64 -0.5 1,743 + GFF2 0.74 -0.3 1,743 + GFF2 0.74 -0.2 1,748 + GFF2 0.75 -0.6 1,749 + GFF2 0.75 -0.5 1,751 - GFF2 0.75 -1.0 1,760 + GFF2 0.76 -0.8 1,766 - GFF2 0.77 -0.8 1,781 + GFF2 0.79 +0.0 1,786 + GFF2 0.80 +0.3 1,796 + GFF2 0.81 +0.2 1,845 - GFF2 0.87 +0.7 1,857 + GFF2 0.89 -1.1 1,896 + +0.6 2,045 - +0.7 2,063 - -1.2 2,071 + +0.4 2,071 + -0.1 2,071 + +0.3 2,071 + -1.0 2,071 + -0.8 2,071 + -0.6 2,071 + -0.2 2,071 + +0.2 2,071 + +0.5 2,071 + -1.4 2,071 + -0.4 2,071 + +0.5 2,071 + -0.2 2,071 + +0.5 2,071 + -1.4 2,071 + -1.2 2,079 - -0.5 2,079 - -0.4 2,079 - -1.6 2,079 - -0.7 2,079 - -0.8 2,079 - +0.3 2,079 - +0.0 2,079 - -0.4 2,079 - +1.1 2,079 - -0.4 2,079 - -0.2 2,079 - -0.4 2,079 - +0.3 2,079 - -0.2 2,079 - -0.4 2,079 - -0.0 2,079 - +0.0 2,079 - -0.2 2,079 - -1.5 2,079 - +1.1 2,079 - -0.5 2,079 - -0.8 2,079 - -0.4 2,079 - -0.5 2,079 - +0.3 2,079 - +0.2 2,079 - +0.0 2,079 - -1.5 2,079 - -0.2 2,079 - -0.3 2,079 - -0.6 2,079 - +0.5 2,079 - -0.5 2,079 - +0.6 2,079 - -1.3 2,079 - +0.6 2,081 + -1.1 2,081 - -0.3 2,088 + -0.7 2,113 - -1.1
Or see this region's nucleotide sequence