Experiment: EMM_noCarbon with D-Glucose and Ammonium Sulfate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF532 and GFF533 are separated by 10 nucleotides GFF533 and GFF534 are separated by 13 nucleotides GFF534 and GFF535 overlap by 1 nucleotides GFF535 and GFF536 are separated by 14 nucleotides GFF536 and GFF537 are separated by 3 nucleotides
GFF532 - Benzoate 1,2-dioxygenase beta subunit (EC 1.14.12.10), at 529,669 to 530,184
GFF532
GFF533 - 1,2-dihydroxycyclohexa-3,5-diene-1-carboxylate dehydrogenase (EC 1.3.1.25), at 530,195 to 530,980
GFF533
GFF534 - Succinyl-CoA:3-ketoacid-coenzyme A transferase subunit A (EC 2.8.3.5), at 530,994 to 531,701
GFF534
GFF535 - Succinyl-CoA:3-ketoacid-coenzyme A transferase subunit B (EC 2.8.3.5), at 531,701 to 532,348
GFF535
GFF536 - Ferredoxin, 2Fe-2S, at 532,363 to 532,680
GFF536
GFF537 - Beta-ketoadipate enol-lactone hydrolase (EC 3.1.1.24), at 532,684 to 533,454
GFF537
Position (kb)
530
531
532 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 530.024 kb on - strand, within GFF532 at 530.033 kb on + strand, within GFF532 at 530.033 kb on + strand, within GFF532 at 530.035 kb on - strand, within GFF532 at 530.102 kb on - strand, within GFF532 at 530.180 kb on + strand at 530.224 kb on + strand at 530.230 kb on + strand at 530.232 kb on - strand at 530.232 kb on - strand at 530.232 kb on - strand at 530.239 kb on - strand at 530.246 kb on + strand at 530.251 kb on + strand at 530.251 kb on + strand at 530.254 kb on - strand at 530.254 kb on - strand at 530.254 kb on - strand at 530.259 kb on - strand at 530.269 kb on + strand at 530.275 kb on - strand, within GFF533 at 530.275 kb on - strand, within GFF533 at 530.290 kb on - strand, within GFF533 at 530.292 kb on + strand, within GFF533 at 530.306 kb on - strand, within GFF533 at 530.308 kb on + strand, within GFF533 at 530.352 kb on - strand, within GFF533 at 530.352 kb on - strand, within GFF533 at 530.357 kb on + strand, within GFF533 at 530.390 kb on + strand, within GFF533 at 530.390 kb on + strand, within GFF533 at 530.398 kb on - strand, within GFF533 at 530.398 kb on - strand, within GFF533 at 530.416 kb on + strand, within GFF533 at 530.424 kb on - strand, within GFF533 at 530.424 kb on - strand, within GFF533 at 530.438 kb on + strand, within GFF533 at 530.446 kb on - strand, within GFF533 at 530.483 kb on + strand, within GFF533 at 530.494 kb on + strand, within GFF533 at 530.496 kb on + strand, within GFF533 at 530.496 kb on + strand, within GFF533 at 530.496 kb on + strand, within GFF533 at 530.504 kb on - strand, within GFF533 at 530.504 kb on - strand, within GFF533 at 530.504 kb on - strand, within GFF533 at 530.511 kb on + strand, within GFF533 at 530.516 kb on + strand, within GFF533 at 530.516 kb on + strand, within GFF533 at 530.524 kb on - strand, within GFF533 at 530.550 kb on + strand, within GFF533 at 530.558 kb on - strand, within GFF533 at 530.558 kb on - strand, within GFF533 at 530.560 kb on + strand, within GFF533 at 530.571 kb on + strand, within GFF533 at 530.589 kb on + strand, within GFF533 at 530.597 kb on - strand, within GFF533 at 530.607 kb on - strand, within GFF533 at 530.622 kb on - strand, within GFF533 at 530.626 kb on + strand, within GFF533 at 530.641 kb on - strand, within GFF533 at 530.656 kb on + strand, within GFF533 at 530.658 kb on - strand, within GFF533 at 530.674 kb on + strand, within GFF533 at 530.674 kb on + strand, within GFF533 at 530.683 kb on - strand, within GFF533 at 530.687 kb on + strand, within GFF533 at 530.695 kb on - strand, within GFF533 at 530.715 kb on + strand, within GFF533 at 530.722 kb on + strand, within GFF533 at 530.758 kb on + strand, within GFF533 at 530.760 kb on + strand, within GFF533 at 530.763 kb on + strand, within GFF533 at 530.766 kb on - strand, within GFF533 at 530.768 kb on + strand, within GFF533 at 530.771 kb on - strand, within GFF533 at 530.771 kb on - strand, within GFF533 at 530.771 kb on - strand, within GFF533 at 530.771 kb on - strand, within GFF533 at 530.771 kb on - strand, within GFF533 at 530.771 kb on - strand, within GFF533 at 530.771 kb on - strand, within GFF533 at 530.793 kb on - strand, within GFF533 at 530.805 kb on + strand, within GFF533 at 530.806 kb on - strand, within GFF533 at 530.818 kb on + strand, within GFF533 at 530.826 kb on - strand, within GFF533 at 530.852 kb on + strand, within GFF533 at 530.893 kb on + strand, within GFF533 at 530.916 kb on + strand at 530.916 kb on + strand at 530.921 kb on + strand at 530.924 kb on - strand at 530.924 kb on - strand at 530.924 kb on - strand at 530.943 kb on + strand at 530.943 kb on + strand at 530.951 kb on - strand at 530.951 kb on - strand at 530.951 kb on - strand at 530.955 kb on - strand at 530.956 kb on - strand at 530.956 kb on - strand at 530.991 kb on - strand at 531.033 kb on + strand at 531.070 kb on + strand, within GFF534 at 531.070 kb on + strand, within GFF534 at 531.070 kb on + strand, within GFF534 at 531.070 kb on + strand, within GFF534 at 531.070 kb on + strand, within GFF534 at 531.078 kb on - strand, within GFF534 at 531.078 kb on - strand, within GFF534 at 531.078 kb on - strand, within GFF534 at 531.078 kb on - strand, within GFF534 at 531.078 kb on - strand, within GFF534 at 531.088 kb on + strand, within GFF534 at 531.091 kb on + strand, within GFF534 at 531.169 kb on + strand, within GFF534 at 531.169 kb on + strand, within GFF534 at 531.220 kb on + strand, within GFF534 at 531.228 kb on - strand, within GFF534 at 531.235 kb on + strand, within GFF534 at 531.243 kb on - strand, within GFF534 at 531.245 kb on + strand, within GFF534 at 531.291 kb on - strand, within GFF534 at 531.345 kb on + strand, within GFF534 at 531.367 kb on + strand, within GFF534 at 531.439 kb on + strand, within GFF534 at 531.439 kb on + strand, within GFF534 at 531.439 kb on + strand, within GFF534 at 531.439 kb on + strand, within GFF534 at 531.444 kb on + strand, within GFF534 at 531.447 kb on - strand, within GFF534 at 531.452 kb on - strand, within GFF534 at 531.463 kb on - strand, within GFF534 at 531.497 kb on + strand, within GFF534 at 531.497 kb on + strand, within GFF534 at 531.497 kb on + strand, within GFF534 at 531.497 kb on + strand, within GFF534 at 531.505 kb on - strand, within GFF534 at 531.505 kb on - strand, within GFF534 at 531.522 kb on - strand, within GFF534 at 531.545 kb on + strand, within GFF534 at 531.545 kb on + strand, within GFF534 at 531.545 kb on + strand, within GFF534 at 531.550 kb on + strand, within GFF534 at 531.553 kb on - strand, within GFF534 at 531.554 kb on + strand, within GFF534 at 531.581 kb on + strand, within GFF534 at 531.584 kb on - strand, within GFF534 at 531.587 kb on + strand, within GFF534 at 531.594 kb on - strand, within GFF534 at 531.594 kb on - strand, within GFF534 at 531.601 kb on - strand, within GFF534 at 531.612 kb on + strand, within GFF534 at 531.636 kb on + strand at 531.644 kb on - strand at 531.647 kb on - strand at 531.777 kb on - strand, within GFF535 at 531.810 kb on - strand, within GFF535 at 531.822 kb on + strand, within GFF535 at 531.824 kb on - strand, within GFF535 at 531.873 kb on - strand, within GFF535 at 531.873 kb on - strand, within GFF535 at 531.887 kb on + strand, within GFF535 at 531.887 kb on + strand, within GFF535 at 531.888 kb on + strand, within GFF535 at 531.888 kb on + strand, within GFF535 at 531.896 kb on - strand, within GFF535 at 531.898 kb on + strand, within GFF535 at 531.901 kb on - strand, within GFF535 at 531.901 kb on - strand, within GFF535 at 531.906 kb on - strand, within GFF535 at 531.932 kb on - strand, within GFF535 at 531.956 kb on + strand, within GFF535 at 531.963 kb on + strand, within GFF535 at 531.964 kb on - strand, within GFF535 at 532.003 kb on + strand, within GFF535 at 532.043 kb on + strand, within GFF535 at 532.066 kb on + strand, within GFF535 at 532.073 kb on + strand, within GFF535 at 532.073 kb on + strand, within GFF535 at 532.080 kb on - strand, within GFF535 at 532.081 kb on - strand, within GFF535 at 532.090 kb on + strand, within GFF535 at 532.096 kb on + strand, within GFF535 at 532.104 kb on - strand, within GFF535 at 532.124 kb on + strand, within GFF535 at 532.162 kb on - strand, within GFF535 at 532.186 kb on - strand, within GFF535 at 532.203 kb on - strand, within GFF535 at 532.218 kb on - strand, within GFF535 at 532.250 kb on - strand, within GFF535 at 532.254 kb on - strand, within GFF535 at 532.256 kb on + strand, within GFF535 at 532.257 kb on + strand, within GFF535 at 532.257 kb on + strand, within GFF535 at 532.265 kb on - strand, within GFF535 at 532.265 kb on - strand, within GFF535 at 532.265 kb on - strand, within GFF535 at 532.265 kb on - strand, within GFF535 at 532.265 kb on - strand, within GFF535 at 532.265 kb on - strand, within GFF535 at 532.265 kb on - strand, within GFF535 at 532.265 kb on - strand, within GFF535 at 532.265 kb on - strand, within GFF535 at 532.279 kb on - strand, within GFF535 at 532.363 kb on - strand at 532.373 kb on - strand at 532.378 kb on + strand at 532.378 kb on + strand at 532.428 kb on + strand, within GFF536 at 532.431 kb on - strand, within GFF536 at 532.442 kb on - strand, within GFF536 at 532.442 kb on - strand, within GFF536 at 532.464 kb on - strand, within GFF536 at 532.465 kb on - strand, within GFF536 at 532.479 kb on + strand, within GFF536 at 532.527 kb on + strand, within GFF536 at 532.527 kb on + strand, within GFF536 at 532.535 kb on - strand, within GFF536 at 532.535 kb on - strand, within GFF536 at 532.573 kb on - strand, within GFF536 at 532.609 kb on + strand, within GFF536 at 532.609 kb on + strand, within GFF536 at 532.617 kb on - strand, within GFF536 at 532.623 kb on + strand, within GFF536 at 532.651 kb on - strand at 532.656 kb on - strand at 532.674 kb on + strand at 532.674 kb on + strand at 532.682 kb on - strand at 532.688 kb on + strand at 532.688 kb on + strand at 532.688 kb on + strand at 532.688 kb on + strand at 532.689 kb on - strand at 532.689 kb on - strand at 532.689 kb on - strand at 532.693 kb on + strand at 532.696 kb on - strand at 532.696 kb on - strand at 532.696 kb on - strand at 532.696 kb on - strand at 532.698 kb on + strand at 532.698 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with D-Glucose and Ammonium Sulfate remove 530,024 - GFF532 0.69 -0.9 530,033 + GFF532 0.71 -0.5 530,033 + GFF532 0.71 -1.2 530,035 - GFF532 0.71 +1.4 530,102 - GFF532 0.84 -1.9 530,180 + +0.0 530,224 + +0.9 530,230 + +0.2 530,232 - -0.1 530,232 - -2.2 530,232 - -0.9 530,239 - +1.3 530,246 + -0.1 530,251 + +1.3 530,251 + -0.2 530,254 - -1.2 530,254 - +0.2 530,254 - +1.0 530,259 - +0.9 530,269 + -1.0 530,275 - GFF533 0.10 +1.1 530,275 - GFF533 0.10 -0.8 530,290 - GFF533 0.12 +0.0 530,292 + GFF533 0.12 -0.0 530,306 - GFF533 0.14 -1.0 530,308 + GFF533 0.14 -0.5 530,352 - GFF533 0.20 +0.1 530,352 - GFF533 0.20 +0.9 530,357 + GFF533 0.21 -1.4 530,390 + GFF533 0.25 +0.5 530,390 + GFF533 0.25 -0.1 530,398 - GFF533 0.26 +0.5 530,398 - GFF533 0.26 -0.9 530,416 + GFF533 0.28 +1.7 530,424 - GFF533 0.29 -1.0 530,424 - GFF533 0.29 +1.0 530,438 + GFF533 0.31 +0.1 530,446 - GFF533 0.32 +1.1 530,483 + GFF533 0.37 -1.1 530,494 + GFF533 0.38 +1.1 530,496 + GFF533 0.38 -0.1 530,496 + GFF533 0.38 -1.6 530,496 + GFF533 0.38 -0.6 530,504 - GFF533 0.39 +0.2 530,504 - GFF533 0.39 -0.7 530,504 - GFF533 0.39 -0.2 530,511 + GFF533 0.40 -0.9 530,516 + GFF533 0.41 +1.4 530,516 + GFF533 0.41 -0.2 530,524 - GFF533 0.42 -1.2 530,550 + GFF533 0.45 -0.5 530,558 - GFF533 0.46 +1.0 530,558 - GFF533 0.46 -0.3 530,560 + GFF533 0.46 +0.0 530,571 + GFF533 0.48 -0.2 530,589 + GFF533 0.50 -1.4 530,597 - GFF533 0.51 +1.4 530,607 - GFF533 0.52 +0.1 530,622 - GFF533 0.54 -0.1 530,626 + GFF533 0.55 +0.4 530,641 - GFF533 0.57 +0.5 530,656 + GFF533 0.59 +1.1 530,658 - GFF533 0.59 +0.2 530,674 + GFF533 0.61 +0.2 530,674 + GFF533 0.61 -0.4 530,683 - GFF533 0.62 -0.0 530,687 + GFF533 0.63 +1.8 530,695 - GFF533 0.64 -0.1 530,715 + GFF533 0.66 +2.2 530,722 + GFF533 0.67 +0.9 530,758 + GFF533 0.72 +0.1 530,760 + GFF533 0.72 +0.5 530,763 + GFF533 0.72 -1.8 530,766 - GFF533 0.73 +0.6 530,768 + GFF533 0.73 -0.4 530,771 - GFF533 0.73 +0.4 530,771 - GFF533 0.73 +0.6 530,771 - GFF533 0.73 +0.2 530,771 - GFF533 0.73 -0.6 530,771 - GFF533 0.73 +0.9 530,771 - GFF533 0.73 +0.9 530,771 - GFF533 0.73 +1.0 530,793 - GFF533 0.76 +0.2 530,805 + GFF533 0.78 -0.1 530,806 - GFF533 0.78 -0.4 530,818 + GFF533 0.79 +0.1 530,826 - GFF533 0.80 +0.4 530,852 + GFF533 0.84 -0.0 530,893 + GFF533 0.89 -0.1 530,916 + +1.2 530,916 + -1.7 530,921 + +1.9 530,924 - -0.7 530,924 - +0.2 530,924 - +1.4 530,943 + +0.2 530,943 + +0.8 530,951 - -0.6 530,951 - +0.1 530,951 - +0.7 530,955 - +1.7 530,956 - -0.4 530,956 - +0.3 530,991 - +1.2 531,033 + -0.3 531,070 + GFF534 0.11 +1.3 531,070 + GFF534 0.11 -0.0 531,070 + GFF534 0.11 +0.4 531,070 + GFF534 0.11 +1.0 531,070 + GFF534 0.11 +0.0 531,078 - GFF534 0.12 -0.9 531,078 - GFF534 0.12 +0.8 531,078 - GFF534 0.12 -0.8 531,078 - GFF534 0.12 -2.0 531,078 - GFF534 0.12 +0.6 531,088 + GFF534 0.13 -0.1 531,091 + GFF534 0.14 -1.5 531,169 + GFF534 0.25 +0.9 531,169 + GFF534 0.25 +0.2 531,220 + GFF534 0.32 -0.2 531,228 - GFF534 0.33 +1.4 531,235 + GFF534 0.34 +0.6 531,243 - GFF534 0.35 -0.5 531,245 + GFF534 0.35 -0.5 531,291 - GFF534 0.42 -0.2 531,345 + GFF534 0.50 -0.4 531,367 + GFF534 0.53 -0.5 531,439 + GFF534 0.63 +0.8 531,439 + GFF534 0.63 -0.5 531,439 + GFF534 0.63 +0.8 531,439 + GFF534 0.63 +1.2 531,444 + GFF534 0.64 -0.6 531,447 - GFF534 0.64 -1.8 531,452 - GFF534 0.65 -1.5 531,463 - GFF534 0.66 -0.4 531,497 + GFF534 0.71 +1.4 531,497 + GFF534 0.71 +0.9 531,497 + GFF534 0.71 -0.2 531,497 + GFF534 0.71 +0.6 531,505 - GFF534 0.72 -0.6 531,505 - GFF534 0.72 -0.9 531,522 - GFF534 0.75 +0.2 531,545 + GFF534 0.78 +0.3 531,545 + GFF534 0.78 -0.4 531,545 + GFF534 0.78 +0.6 531,550 + GFF534 0.79 -1.0 531,553 - GFF534 0.79 -1.4 531,554 + GFF534 0.79 +1.2 531,581 + GFF534 0.83 -1.8 531,584 - GFF534 0.83 -0.1 531,587 + GFF534 0.84 -1.1 531,594 - GFF534 0.85 +1.5 531,594 - GFF534 0.85 +0.7 531,601 - GFF534 0.86 +0.9 531,612 + GFF534 0.87 -0.0 531,636 + -0.5 531,644 - -0.4 531,647 - +1.1 531,777 - GFF535 0.12 +0.6 531,810 - GFF535 0.17 -0.6 531,822 + GFF535 0.19 -0.1 531,824 - GFF535 0.19 +0.4 531,873 - GFF535 0.27 +0.1 531,873 - GFF535 0.27 +0.2 531,887 + GFF535 0.29 -0.1 531,887 + GFF535 0.29 +0.5 531,888 + GFF535 0.29 -0.6 531,888 + GFF535 0.29 +0.1 531,896 - GFF535 0.30 -0.1 531,898 + GFF535 0.30 +0.7 531,901 - GFF535 0.31 -1.5 531,901 - GFF535 0.31 +0.1 531,906 - GFF535 0.32 -0.7 531,932 - GFF535 0.36 +0.7 531,956 + GFF535 0.39 -1.2 531,963 + GFF535 0.40 +0.4 531,964 - GFF535 0.41 +0.5 532,003 + GFF535 0.47 +0.9 532,043 + GFF535 0.53 -1.3 532,066 + GFF535 0.56 -0.2 532,073 + GFF535 0.57 +0.1 532,073 + GFF535 0.57 +0.6 532,080 - GFF535 0.58 -1.0 532,081 - GFF535 0.59 -0.3 532,090 + GFF535 0.60 -3.2 532,096 + GFF535 0.61 -2.8 532,104 - GFF535 0.62 +0.2 532,124 + GFF535 0.65 +0.1 532,162 - GFF535 0.71 -0.5 532,186 - GFF535 0.75 +0.6 532,203 - GFF535 0.77 -0.9 532,218 - GFF535 0.80 -0.5 532,250 - GFF535 0.85 -0.0 532,254 - GFF535 0.85 +0.2 532,256 + GFF535 0.86 +0.6 532,257 + GFF535 0.86 +0.6 532,257 + GFF535 0.86 +0.5 532,265 - GFF535 0.87 -0.7 532,265 - GFF535 0.87 +0.1 532,265 - GFF535 0.87 -0.1 532,265 - GFF535 0.87 -1.5 532,265 - GFF535 0.87 +0.6 532,265 - GFF535 0.87 -1.4 532,265 - GFF535 0.87 -1.5 532,265 - GFF535 0.87 +0.6 532,265 - GFF535 0.87 +0.4 532,279 - GFF535 0.89 -0.6 532,363 - -0.3 532,373 - -1.3 532,378 + +0.4 532,378 + +1.6 532,428 + GFF536 0.20 +0.7 532,431 - GFF536 0.21 +0.9 532,442 - GFF536 0.25 +1.2 532,442 - GFF536 0.25 -0.7 532,464 - GFF536 0.32 -0.4 532,465 - GFF536 0.32 -1.9 532,479 + GFF536 0.36 -2.0 532,527 + GFF536 0.52 +1.1 532,527 + GFF536 0.52 -0.6 532,535 - GFF536 0.54 -1.5 532,535 - GFF536 0.54 +0.5 532,573 - GFF536 0.66 +0.2 532,609 + GFF536 0.77 -1.2 532,609 + GFF536 0.77 +0.0 532,617 - GFF536 0.80 -1.5 532,623 + GFF536 0.82 -1.0 532,651 - -0.5 532,656 - -0.9 532,674 + +0.1 532,674 + -1.3 532,682 - +0.6 532,688 + -1.5 532,688 + -0.9 532,688 + +0.2 532,688 + +0.3 532,689 - -0.1 532,689 - -1.1 532,689 - +0.7 532,693 + -0.6 532,696 - +0.3 532,696 - +0.6 532,696 - +0.2 532,696 - -0.5 532,698 + -0.1 532,698 + -0.8
Or see this region's nucleotide sequence