Experiment: EMM_noCarbon with D-Glucose and Ammonium Sulfate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF50 and GFF51 are separated by 50 nucleotides GFF51 and GFF52 are separated by 28 nucleotides
GFF50 - Phosphoglucomutase (EC 5.4.2.2), at 40,124 to 41,752
GFF50
GFF51 - hypothetical protein, at 41,803 to 41,949
GFF51
GFF52 - Choloylglycine hydrolase (EC 3.5.1.24), at 41,978 to 43,033
GFF52
Position (kb)
41
42 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 41.207 kb on - strand, within GFF50 at 41.418 kb on - strand, within GFF50 at 41.608 kb on - strand at 41.742 kb on + strand at 41.750 kb on - strand at 41.750 kb on - strand at 41.750 kb on - strand at 41.750 kb on - strand at 41.752 kb on + strand at 41.760 kb on - strand at 41.760 kb on - strand at 41.760 kb on - strand at 41.760 kb on - strand at 41.760 kb on - strand at 41.760 kb on - strand at 41.765 kb on - strand at 41.772 kb on - strand at 41.795 kb on - strand at 41.803 kb on + strand at 41.803 kb on + strand at 41.803 kb on + strand at 41.808 kb on + strand at 41.813 kb on + strand at 41.816 kb on - strand at 41.816 kb on - strand at 41.883 kb on - strand, within GFF51 at 41.894 kb on - strand, within GFF51 at 41.894 kb on - strand, within GFF51 at 41.904 kb on + strand, within GFF51 at 41.944 kb on + strand at 41.950 kb on + strand at 41.950 kb on + strand at 41.958 kb on - strand at 41.981 kb on - strand at 42.006 kb on + strand at 42.006 kb on + strand at 42.006 kb on + strand at 42.024 kb on - strand at 42.031 kb on - strand at 42.102 kb on + strand, within GFF52 at 42.136 kb on + strand, within GFF52 at 42.144 kb on - strand, within GFF52 at 42.144 kb on - strand, within GFF52 at 42.144 kb on - strand, within GFF52 at 42.156 kb on + strand, within GFF52 at 42.156 kb on + strand, within GFF52 at 42.176 kb on - strand, within GFF52 at 42.180 kb on + strand, within GFF52 at 42.264 kb on + strand, within GFF52 at 42.264 kb on + strand, within GFF52 at 42.264 kb on + strand, within GFF52 at 42.264 kb on + strand, within GFF52 at 42.264 kb on + strand, within GFF52 at 42.272 kb on - strand, within GFF52 at 42.272 kb on - strand, within GFF52 at 42.272 kb on - strand, within GFF52 at 42.335 kb on + strand, within GFF52 at 42.396 kb on - strand, within GFF52 at 42.475 kb on - strand, within GFF52 at 42.483 kb on + strand, within GFF52 at 42.494 kb on + strand, within GFF52 at 42.558 kb on + strand, within GFF52 at 42.609 kb on - strand, within GFF52 at 42.655 kb on + strand, within GFF52 at 42.662 kb on + strand, within GFF52 at 42.692 kb on + strand, within GFF52 at 42.692 kb on - strand, within GFF52 at 42.709 kb on + strand, within GFF52 at 42.711 kb on + strand, within GFF52 at 42.716 kb on + strand, within GFF52 at 42.724 kb on - strand, within GFF52 at 42.728 kb on + strand, within GFF52 at 42.728 kb on + strand, within GFF52 at 42.728 kb on + strand, within GFF52 at 42.728 kb on + strand, within GFF52 at 42.736 kb on - strand, within GFF52 at 42.737 kb on + strand, within GFF52 at 42.741 kb on - strand, within GFF52 at 42.767 kb on + strand, within GFF52 at 42.796 kb on + strand, within GFF52 at 42.804 kb on - strand, within GFF52 at 42.804 kb on - strand, within GFF52 at 42.835 kb on + strand, within GFF52 at 42.835 kb on + strand, within GFF52 at 42.883 kb on + strand, within GFF52 at 42.894 kb on + strand, within GFF52 at 42.894 kb on + strand, within GFF52 at 42.895 kb on + strand, within GFF52 at 42.909 kb on + strand, within GFF52 at 42.909 kb on + strand, within GFF52 at 42.909 kb on + strand, within GFF52 at 42.917 kb on - strand, within GFF52 at 42.917 kb on - strand, within GFF52
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with D-Glucose and Ammonium Sulfate remove 41,207 - GFF50 0.66 -0.3 41,418 - GFF50 0.79 +1.3 41,608 - +0.3 41,742 + +0.3 41,750 - -0.1 41,750 - +0.2 41,750 - -1.0 41,750 - -1.6 41,752 + -0.9 41,760 - -1.1 41,760 - +0.4 41,760 - -1.0 41,760 - +0.1 41,760 - -0.2 41,760 - +0.3 41,765 - -0.9 41,772 - +0.6 41,795 - -1.6 41,803 + -0.5 41,803 + -0.4 41,803 + +0.2 41,808 + +0.4 41,813 + -0.8 41,816 - -1.3 41,816 - +0.3 41,883 - GFF51 0.54 +1.4 41,894 - GFF51 0.62 -1.0 41,894 - GFF51 0.62 +0.5 41,904 + GFF51 0.69 -1.9 41,944 + -1.3 41,950 + -0.8 41,950 + +0.0 41,958 - +0.8 41,981 - -0.1 42,006 + +0.5 42,006 + -0.1 42,006 + -1.0 42,024 - +0.3 42,031 - -0.4 42,102 + GFF52 0.12 +0.3 42,136 + GFF52 0.15 -0.9 42,144 - GFF52 0.16 -1.3 42,144 - GFF52 0.16 +0.3 42,144 - GFF52 0.16 +0.9 42,156 + GFF52 0.17 +0.0 42,156 + GFF52 0.17 -0.7 42,176 - GFF52 0.19 +0.5 42,180 + GFF52 0.19 +0.2 42,264 + GFF52 0.27 +0.3 42,264 + GFF52 0.27 -0.1 42,264 + GFF52 0.27 -0.4 42,264 + GFF52 0.27 +0.3 42,264 + GFF52 0.27 +2.8 42,272 - GFF52 0.28 -1.4 42,272 - GFF52 0.28 +0.5 42,272 - GFF52 0.28 +0.7 42,335 + GFF52 0.34 +0.9 42,396 - GFF52 0.40 +0.2 42,475 - GFF52 0.47 +0.8 42,483 + GFF52 0.48 -2.5 42,494 + GFF52 0.49 -0.3 42,558 + GFF52 0.55 -0.4 42,609 - GFF52 0.60 +1.1 42,655 + GFF52 0.64 +1.0 42,662 + GFF52 0.65 +1.9 42,692 + GFF52 0.68 +0.4 42,692 - GFF52 0.68 -1.5 42,709 + GFF52 0.69 -1.0 42,711 + GFF52 0.69 -1.2 42,716 + GFF52 0.70 +0.9 42,724 - GFF52 0.71 +0.7 42,728 + GFF52 0.71 +0.4 42,728 + GFF52 0.71 -0.5 42,728 + GFF52 0.71 -1.7 42,728 + GFF52 0.71 +0.3 42,736 - GFF52 0.72 +0.9 42,737 + GFF52 0.72 +0.1 42,741 - GFF52 0.72 +0.0 42,767 + GFF52 0.75 +0.9 42,796 + GFF52 0.77 +0.0 42,804 - GFF52 0.78 +1.4 42,804 - GFF52 0.78 -0.3 42,835 + GFF52 0.81 -1.2 42,835 + GFF52 0.81 -1.7 42,883 + GFF52 0.86 +0.0 42,894 + GFF52 0.87 +0.6 42,894 + GFF52 0.87 +0.0 42,895 + GFF52 0.87 +1.6 42,909 + GFF52 0.88 -0.1 42,909 + GFF52 0.88 +0.1 42,909 + GFF52 0.88 -0.6 42,917 - GFF52 0.89 -1.2 42,917 - GFF52 0.89 +0.5
Or see this region's nucleotide sequence