Experiment: EMM_noCarbon with D-Glucose and Ammonium Sulfate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5227 and GFF5228 are separated by 96 nucleotides GFF5228 and GFF5229 are separated by 142 nucleotides GFF5229 and GFF5230 are separated by 31 nucleotides GFF5230 and GFF5231 are separated by 281 nucleotides
GFF5227 - hypothetical protein, at 5,220,356 to 5,221,024
GFF5227
GFF5228 - Ribose-phosphate pyrophosphokinase, possible alternative form 2, at 5,221,121 to 5,221,429
GFF5228
GFF5229 - Integrase, at 5,221,572 to 5,221,919
GFF5229
GFF5230 - hypothetical protein, at 5,221,951 to 5,222,292
GFF5230
GFF5231 - hypothetical protein, at 5,222,574 to 5,223,980
GFF5231
Position (kb)
5221
5222 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5220.572 kb on + strand, within GFF5227 at 5220.641 kb on - strand, within GFF5227 at 5220.692 kb on + strand, within GFF5227 at 5220.692 kb on + strand, within GFF5227 at 5220.700 kb on + strand, within GFF5227 at 5220.721 kb on - strand, within GFF5227 at 5220.721 kb on - strand, within GFF5227 at 5220.731 kb on - strand, within GFF5227 at 5220.732 kb on + strand, within GFF5227 at 5220.740 kb on - strand, within GFF5227 at 5220.771 kb on - strand, within GFF5227 at 5220.773 kb on + strand, within GFF5227 at 5220.821 kb on - strand, within GFF5227 at 5220.831 kb on - strand, within GFF5227 at 5220.865 kb on + strand, within GFF5227 at 5220.899 kb on - strand, within GFF5227 at 5220.912 kb on - strand, within GFF5227 at 5220.912 kb on - strand, within GFF5227 at 5220.922 kb on - strand, within GFF5227 at 5220.949 kb on - strand, within GFF5227 at 5220.952 kb on + strand, within GFF5227 at 5221.012 kb on - strand at 5221.018 kb on + strand at 5221.018 kb on + strand at 5221.018 kb on + strand at 5221.019 kb on + strand at 5221.024 kb on + strand at 5221.026 kb on - strand at 5221.026 kb on - strand at 5221.026 kb on - strand at 5221.026 kb on - strand at 5221.026 kb on - strand at 5221.026 kb on - strand at 5221.033 kb on - strand at 5221.084 kb on + strand at 5221.084 kb on + strand at 5221.087 kb on - strand at 5221.099 kb on - strand at 5221.134 kb on + strand at 5221.171 kb on + strand, within GFF5228 at 5221.171 kb on + strand, within GFF5228 at 5221.171 kb on + strand, within GFF5228 at 5221.171 kb on + strand, within GFF5228 at 5221.171 kb on + strand, within GFF5228 at 5221.171 kb on + strand, within GFF5228 at 5221.171 kb on + strand, within GFF5228 at 5221.176 kb on + strand, within GFF5228 at 5221.179 kb on - strand, within GFF5228 at 5221.179 kb on - strand, within GFF5228 at 5221.179 kb on - strand, within GFF5228 at 5221.179 kb on - strand, within GFF5228 at 5221.179 kb on - strand, within GFF5228 at 5221.179 kb on - strand, within GFF5228 at 5221.179 kb on - strand, within GFF5228 at 5221.181 kb on + strand, within GFF5228 at 5221.186 kb on - strand, within GFF5228 at 5221.217 kb on - strand, within GFF5228 at 5221.223 kb on + strand, within GFF5228 at 5221.223 kb on + strand, within GFF5228 at 5221.223 kb on + strand, within GFF5228 at 5221.229 kb on + strand, within GFF5228 at 5221.229 kb on + strand, within GFF5228 at 5221.231 kb on - strand, within GFF5228 at 5221.237 kb on - strand, within GFF5228 at 5221.237 kb on - strand, within GFF5228 at 5221.237 kb on - strand, within GFF5228 at 5221.242 kb on - strand, within GFF5228 at 5221.550 kb on + strand at 5221.562 kb on + strand at 5221.620 kb on + strand, within GFF5229 at 5221.628 kb on - strand, within GFF5229 at 5221.628 kb on - strand, within GFF5229 at 5221.640 kb on + strand, within GFF5229 at 5221.643 kb on + strand, within GFF5229 at 5221.648 kb on - strand, within GFF5229 at 5221.648 kb on - strand, within GFF5229 at 5221.651 kb on - strand, within GFF5229 at 5221.651 kb on - strand, within GFF5229 at 5221.651 kb on - strand, within GFF5229 at 5221.687 kb on + strand, within GFF5229 at 5221.694 kb on - strand, within GFF5229 at 5221.728 kb on + strand, within GFF5229 at 5221.738 kb on + strand, within GFF5229 at 5221.746 kb on - strand, within GFF5229 at 5221.746 kb on - strand, within GFF5229 at 5221.748 kb on + strand, within GFF5229 at 5221.756 kb on - strand, within GFF5229 at 5221.760 kb on + strand, within GFF5229 at 5221.763 kb on - strand, within GFF5229 at 5221.763 kb on - strand, within GFF5229 at 5221.778 kb on + strand, within GFF5229 at 5221.789 kb on - strand, within GFF5229 at 5221.789 kb on - strand, within GFF5229 at 5221.789 kb on - strand, within GFF5229 at 5221.814 kb on - strand, within GFF5229 at 5221.837 kb on + strand, within GFF5229 at 5221.869 kb on + strand, within GFF5229 at 5221.883 kb on - strand, within GFF5229 at 5221.883 kb on - strand, within GFF5229 at 5221.892 kb on - strand at 5221.893 kb on - strand at 5221.905 kb on + strand at 5221.913 kb on - strand at 5221.934 kb on - strand at 5221.944 kb on + strand at 5221.944 kb on + strand at 5221.944 kb on + strand at 5221.944 kb on + strand at 5221.947 kb on - strand at 5221.952 kb on - strand at 5221.952 kb on - strand at 5221.952 kb on - strand at 5221.952 kb on - strand at 5221.956 kb on - strand at 5221.956 kb on - strand at 5221.966 kb on - strand at 5221.979 kb on + strand at 5221.993 kb on + strand, within GFF5230 at 5222.035 kb on + strand, within GFF5230 at 5222.076 kb on - strand, within GFF5230 at 5222.076 kb on - strand, within GFF5230 at 5222.077 kb on - strand, within GFF5230 at 5222.085 kb on + strand, within GFF5230 at 5222.085 kb on + strand, within GFF5230 at 5222.085 kb on + strand, within GFF5230 at 5222.085 kb on + strand, within GFF5230 at 5222.085 kb on + strand, within GFF5230 at 5222.093 kb on - strand, within GFF5230 at 5222.093 kb on - strand, within GFF5230 at 5222.093 kb on - strand, within GFF5230 at 5222.101 kb on - strand, within GFF5230 at 5222.117 kb on + strand, within GFF5230 at 5222.118 kb on + strand, within GFF5230 at 5222.122 kb on - strand, within GFF5230 at 5222.126 kb on - strand, within GFF5230 at 5222.127 kb on + strand, within GFF5230 at 5222.137 kb on + strand, within GFF5230 at 5222.145 kb on - strand, within GFF5230 at 5222.176 kb on - strand, within GFF5230 at 5222.185 kb on + strand, within GFF5230 at 5222.185 kb on + strand, within GFF5230 at 5222.186 kb on - strand, within GFF5230 at 5222.190 kb on + strand, within GFF5230 at 5222.190 kb on + strand, within GFF5230 at 5222.190 kb on + strand, within GFF5230 at 5222.193 kb on - strand, within GFF5230 at 5222.193 kb on - strand, within GFF5230 at 5222.195 kb on + strand, within GFF5230 at 5222.195 kb on + strand, within GFF5230 at 5222.195 kb on + strand, within GFF5230 at 5222.209 kb on + strand, within GFF5230 at 5222.212 kb on + strand, within GFF5230 at 5222.255 kb on + strand, within GFF5230 at 5222.255 kb on + strand, within GFF5230 at 5222.297 kb on + strand at 5222.297 kb on + strand at 5222.302 kb on + strand at 5222.302 kb on + strand at 5222.302 kb on + strand at 5222.305 kb on - strand at 5222.305 kb on - strand at 5222.307 kb on - strand at 5222.310 kb on - strand at 5222.310 kb on - strand at 5222.312 kb on + strand at 5222.325 kb on + strand at 5222.342 kb on + strand at 5222.343 kb on + strand at 5222.343 kb on + strand at 5222.386 kb on - strand at 5222.386 kb on - strand at 5222.388 kb on - strand at 5222.445 kb on + strand at 5222.445 kb on + strand at 5222.452 kb on - strand at 5222.466 kb on - strand at 5222.513 kb on + strand at 5222.521 kb on - strand at 5222.542 kb on + strand at 5222.550 kb on - strand at 5222.550 kb on - strand at 5222.550 kb on - strand at 5222.552 kb on + strand at 5222.560 kb on - strand at 5222.569 kb on - strand at 5222.574 kb on - strand at 5222.581 kb on - strand at 5222.589 kb on - strand at 5222.591 kb on + strand at 5222.591 kb on + strand at 5222.594 kb on - strand at 5222.596 kb on + strand at 5222.596 kb on + strand at 5222.596 kb on + strand at 5222.596 kb on + strand at 5222.599 kb on - strand at 5222.599 kb on - strand at 5222.604 kb on - strand at 5222.604 kb on - strand at 5222.604 kb on - strand at 5222.609 kb on - strand at 5222.629 kb on + strand at 5222.639 kb on + strand at 5222.639 kb on + strand at 5222.642 kb on - strand at 5222.642 kb on - strand at 5222.645 kb on - strand at 5222.689 kb on + strand at 5222.689 kb on + strand at 5222.699 kb on - strand at 5222.699 kb on - strand at 5222.713 kb on + strand at 5222.723 kb on - strand, within GFF5231 at 5222.740 kb on - strand, within GFF5231 at 5222.753 kb on + strand, within GFF5231 at 5222.761 kb on - strand, within GFF5231 at 5222.770 kb on + strand, within GFF5231 at 5222.774 kb on - strand, within GFF5231 at 5222.787 kb on + strand, within GFF5231 at 5222.787 kb on + strand, within GFF5231 at 5222.814 kb on - strand, within GFF5231 at 5222.838 kb on - strand, within GFF5231 at 5222.849 kb on + strand, within GFF5231 at 5222.849 kb on + strand, within GFF5231 at 5222.857 kb on - strand, within GFF5231 at 5222.891 kb on + strand, within GFF5231 at 5222.896 kb on + strand, within GFF5231 at 5222.896 kb on + strand, within GFF5231 at 5222.899 kb on - strand, within GFF5231 at 5222.899 kb on - strand, within GFF5231 at 5222.899 kb on - strand, within GFF5231 at 5222.899 kb on - strand, within GFF5231 at 5222.901 kb on + strand, within GFF5231
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with D-Glucose and Ammonium Sulfate remove 5,220,572 + GFF5227 0.32 -1.1 5,220,641 - GFF5227 0.43 -0.7 5,220,692 + GFF5227 0.50 +1.4 5,220,692 + GFF5227 0.50 +0.3 5,220,700 + GFF5227 0.51 +0.6 5,220,721 - GFF5227 0.55 +0.7 5,220,721 - GFF5227 0.55 +0.3 5,220,731 - GFF5227 0.56 +1.5 5,220,732 + GFF5227 0.56 +0.4 5,220,740 - GFF5227 0.57 -1.1 5,220,771 - GFF5227 0.62 -1.0 5,220,773 + GFF5227 0.62 +0.8 5,220,821 - GFF5227 0.70 +0.0 5,220,831 - GFF5227 0.71 +0.3 5,220,865 + GFF5227 0.76 -1.6 5,220,899 - GFF5227 0.81 -0.7 5,220,912 - GFF5227 0.83 -0.4 5,220,912 - GFF5227 0.83 +0.7 5,220,922 - GFF5227 0.85 -2.4 5,220,949 - GFF5227 0.89 +0.0 5,220,952 + GFF5227 0.89 -0.2 5,221,012 - -1.7 5,221,018 + -0.6 5,221,018 + -0.8 5,221,018 + +1.0 5,221,019 + +0.5 5,221,024 + +1.0 5,221,026 - +1.6 5,221,026 - +0.8 5,221,026 - +0.1 5,221,026 - +0.7 5,221,026 - -2.1 5,221,026 - -0.1 5,221,033 - -0.5 5,221,084 + +0.7 5,221,084 + -1.2 5,221,087 - +1.1 5,221,099 - -1.8 5,221,134 + -0.9 5,221,171 + GFF5228 0.16 -0.5 5,221,171 + GFF5228 0.16 -0.3 5,221,171 + GFF5228 0.16 -0.8 5,221,171 + GFF5228 0.16 +0.3 5,221,171 + GFF5228 0.16 +0.9 5,221,171 + GFF5228 0.16 +0.1 5,221,171 + GFF5228 0.16 -0.5 5,221,176 + GFF5228 0.18 +0.5 5,221,179 - GFF5228 0.19 +0.9 5,221,179 - GFF5228 0.19 +0.3 5,221,179 - GFF5228 0.19 -0.4 5,221,179 - GFF5228 0.19 +1.0 5,221,179 - GFF5228 0.19 -0.3 5,221,179 - GFF5228 0.19 -2.5 5,221,179 - GFF5228 0.19 +0.0 5,221,181 + GFF5228 0.19 -0.5 5,221,186 - GFF5228 0.21 +0.6 5,221,217 - GFF5228 0.31 +0.3 5,221,223 + GFF5228 0.33 -1.4 5,221,223 + GFF5228 0.33 +0.6 5,221,223 + GFF5228 0.33 -1.1 5,221,229 + GFF5228 0.35 -0.5 5,221,229 + GFF5228 0.35 +0.4 5,221,231 - GFF5228 0.36 -0.1 5,221,237 - GFF5228 0.38 -0.1 5,221,237 - GFF5228 0.38 +0.7 5,221,237 - GFF5228 0.38 -0.7 5,221,242 - GFF5228 0.39 -1.1 5,221,550 + -0.1 5,221,562 + +0.5 5,221,620 + GFF5229 0.14 -0.5 5,221,628 - GFF5229 0.16 -0.2 5,221,628 - GFF5229 0.16 -0.6 5,221,640 + GFF5229 0.20 +0.9 5,221,643 + GFF5229 0.20 +1.0 5,221,648 - GFF5229 0.22 -0.7 5,221,648 - GFF5229 0.22 +0.6 5,221,651 - GFF5229 0.23 -0.1 5,221,651 - GFF5229 0.23 -1.6 5,221,651 - GFF5229 0.23 -0.0 5,221,687 + GFF5229 0.33 -0.4 5,221,694 - GFF5229 0.35 -1.2 5,221,728 + GFF5229 0.45 -0.1 5,221,738 + GFF5229 0.48 -0.2 5,221,746 - GFF5229 0.50 -0.4 5,221,746 - GFF5229 0.50 +1.5 5,221,748 + GFF5229 0.51 -0.1 5,221,756 - GFF5229 0.53 -1.9 5,221,760 + GFF5229 0.54 -1.0 5,221,763 - GFF5229 0.55 -1.5 5,221,763 - GFF5229 0.55 +1.4 5,221,778 + GFF5229 0.59 -0.6 5,221,789 - GFF5229 0.62 -0.9 5,221,789 - GFF5229 0.62 +0.8 5,221,789 - GFF5229 0.62 -0.1 5,221,814 - GFF5229 0.70 +1.3 5,221,837 + GFF5229 0.76 -0.5 5,221,869 + GFF5229 0.85 -0.4 5,221,883 - GFF5229 0.89 +0.9 5,221,883 - GFF5229 0.89 +0.2 5,221,892 - +1.0 5,221,893 - -0.7 5,221,905 + -0.9 5,221,913 - +0.7 5,221,934 - -0.4 5,221,944 + -1.1 5,221,944 + +0.8 5,221,944 + +0.4 5,221,944 + +0.7 5,221,947 - -2.2 5,221,952 - +0.1 5,221,952 - +0.2 5,221,952 - -0.9 5,221,952 - -0.6 5,221,956 - -1.0 5,221,956 - -0.3 5,221,966 - -1.2 5,221,979 + -1.0 5,221,993 + GFF5230 0.12 -0.5 5,222,035 + GFF5230 0.25 +0.3 5,222,076 - GFF5230 0.37 -0.7 5,222,076 - GFF5230 0.37 -0.6 5,222,077 - GFF5230 0.37 -0.5 5,222,085 + GFF5230 0.39 -1.7 5,222,085 + GFF5230 0.39 -2.2 5,222,085 + GFF5230 0.39 +0.4 5,222,085 + GFF5230 0.39 -0.6 5,222,085 + GFF5230 0.39 +0.2 5,222,093 - GFF5230 0.42 +0.2 5,222,093 - GFF5230 0.42 -0.6 5,222,093 - GFF5230 0.42 -1.6 5,222,101 - GFF5230 0.44 -1.2 5,222,117 + GFF5230 0.49 -0.2 5,222,118 + GFF5230 0.49 -0.6 5,222,122 - GFF5230 0.50 -1.5 5,222,126 - GFF5230 0.51 -0.3 5,222,127 + GFF5230 0.51 +0.6 5,222,137 + GFF5230 0.54 +1.2 5,222,145 - GFF5230 0.57 +0.6 5,222,176 - GFF5230 0.66 +0.3 5,222,185 + GFF5230 0.68 +0.7 5,222,185 + GFF5230 0.68 +0.2 5,222,186 - GFF5230 0.69 +0.7 5,222,190 + GFF5230 0.70 -1.7 5,222,190 + GFF5230 0.70 -0.4 5,222,190 + GFF5230 0.70 +1.8 5,222,193 - GFF5230 0.71 +1.3 5,222,193 - GFF5230 0.71 -1.7 5,222,195 + GFF5230 0.71 -0.1 5,222,195 + GFF5230 0.71 -0.0 5,222,195 + GFF5230 0.71 -1.0 5,222,209 + GFF5230 0.75 +0.2 5,222,212 + GFF5230 0.76 +1.2 5,222,255 + GFF5230 0.89 -1.0 5,222,255 + GFF5230 0.89 +0.4 5,222,297 + +0.3 5,222,297 + -1.0 5,222,302 + +0.3 5,222,302 + -0.6 5,222,302 + -0.3 5,222,305 - +1.0 5,222,305 - -0.9 5,222,307 - -0.1 5,222,310 - -0.4 5,222,310 - +1.1 5,222,312 + +1.2 5,222,325 + +0.3 5,222,342 + -0.8 5,222,343 + +0.4 5,222,343 + -2.2 5,222,386 - -0.2 5,222,386 - +0.9 5,222,388 - +0.1 5,222,445 + +0.1 5,222,445 + +0.1 5,222,452 - -1.1 5,222,466 - -0.4 5,222,513 + -0.3 5,222,521 - -0.4 5,222,542 + -0.2 5,222,550 - -0.0 5,222,550 - -0.2 5,222,550 - -0.6 5,222,552 + -0.7 5,222,560 - +0.0 5,222,569 - -0.4 5,222,574 - -0.7 5,222,581 - -0.9 5,222,589 - -0.5 5,222,591 + +0.9 5,222,591 + +0.8 5,222,594 - +1.5 5,222,596 + -1.1 5,222,596 + +0.0 5,222,596 + -2.0 5,222,596 + -1.1 5,222,599 - -1.1 5,222,599 - -0.0 5,222,604 - +0.1 5,222,604 - -1.4 5,222,604 - -1.2 5,222,609 - +0.4 5,222,629 + -0.6 5,222,639 + -0.7 5,222,639 + +0.3 5,222,642 - -0.1 5,222,642 - +1.1 5,222,645 - -1.7 5,222,689 + -0.3 5,222,689 + -1.9 5,222,699 - -0.5 5,222,699 - -1.3 5,222,713 + -1.1 5,222,723 - GFF5231 0.11 +1.5 5,222,740 - GFF5231 0.12 -0.4 5,222,753 + GFF5231 0.13 +1.4 5,222,761 - GFF5231 0.13 -2.0 5,222,770 + GFF5231 0.14 +1.1 5,222,774 - GFF5231 0.14 +0.3 5,222,787 + GFF5231 0.15 +1.1 5,222,787 + GFF5231 0.15 +0.3 5,222,814 - GFF5231 0.17 -1.0 5,222,838 - GFF5231 0.19 -0.8 5,222,849 + GFF5231 0.20 -0.7 5,222,849 + GFF5231 0.20 -2.1 5,222,857 - GFF5231 0.20 -0.2 5,222,891 + GFF5231 0.23 -0.5 5,222,896 + GFF5231 0.23 +0.7 5,222,896 + GFF5231 0.23 -0.0 5,222,899 - GFF5231 0.23 -0.0 5,222,899 - GFF5231 0.23 +1.4 5,222,899 - GFF5231 0.23 -0.5 5,222,899 - GFF5231 0.23 -0.5 5,222,901 + GFF5231 0.23 +0.2
Or see this region's nucleotide sequence