Experiment: EMM_noCarbon with D-Glucose and Ammonium Sulfate 
Only strains with sufficient reads to estimate fitness are shown,
                   but the strain fitness values are still rather noisy and may be biased towards zero.
                   Strains near the edge of a gene are not shown as being associated with that
                   gene (they are in grey).
                   Strains in the central 10-90% of a gene are color coded by the insertion's strand.
                   Usually, "+" means that the selectable marker is encoded on the forward strand,
                   i.e., transcribed rightward. 
    
        
         
    
        
         
    
        
         
    
        
         
    
         
     
500 nt GFF4736 and GFF4737 overlap by 4 nucleotides GFF4737 and GFF4738 are separated by 163 nucleotides 
        GFF4736 - hypothetical protein, at 4,735,400 to 4,736,563 
        GFF4736 
         
        
        GFF4737 - Efflux ABC transporter, permease protein, at 4,736,560 to 4,737,690 
        GFF4737 
         
        
        GFF4738 - hypothetical protein, at 4,737,854 to 4,738,765 
        GFF4738 
         Position (kb) 4736 
4737 
4738 Strain fitness (log2 ratio) -2 
-1 
0 
1 
2 at 4735.591 kb on - strand, within GFF4736 at 4735.610 kb on - strand, within GFF4736 at 4735.647 kb on + strand, within GFF4736 at 4735.657 kb on + strand, within GFF4736 at 4735.664 kb on + strand, within GFF4736 at 4735.672 kb on - strand, within GFF4736 at 4735.677 kb on - strand, within GFF4736 at 4735.691 kb on + strand, within GFF4736 at 4735.692 kb on + strand, within GFF4736 at 4735.705 kb on - strand, within GFF4736 at 4735.722 kb on + strand, within GFF4736 at 4735.723 kb on - strand, within GFF4736 at 4735.727 kb on + strand, within GFF4736 at 4735.727 kb on + strand, within GFF4736 at 4735.735 kb on - strand, within GFF4736 at 4735.759 kb on + strand, within GFF4736 at 4735.771 kb on + strand, within GFF4736 at 4735.820 kb on + strand, within GFF4736 at 4735.828 kb on - strand, within GFF4736 at 4735.828 kb on - strand, within GFF4736 at 4735.828 kb on - strand, within GFF4736 at 4735.828 kb on - strand, within GFF4736 at 4735.845 kb on - strand, within GFF4736 at 4735.845 kb on - strand, within GFF4736 at 4735.845 kb on - strand, within GFF4736 at 4735.901 kb on + strand, within GFF4736 at 4735.911 kb on + strand, within GFF4736 at 4735.928 kb on + strand, within GFF4736 at 4735.928 kb on + strand, within GFF4736 at 4735.963 kb on + strand, within GFF4736 at 4735.963 kb on + strand, within GFF4736 at 4735.963 kb on + strand, within GFF4736 at 4735.963 kb on + strand, within GFF4736 at 4735.976 kb on + strand, within GFF4736 at 4735.976 kb on + strand, within GFF4736 at 4735.976 kb on + strand, within GFF4736 at 4735.976 kb on + strand, within GFF4736 at 4735.976 kb on + strand, within GFF4736 at 4735.984 kb on - strand, within GFF4736 at 4735.984 kb on - strand, within GFF4736 at 4735.984 kb on - strand, within GFF4736 at 4735.984 kb on - strand, within GFF4736 at 4736.027 kb on - strand, within GFF4736 at 4736.027 kb on - strand, within GFF4736 at 4736.046 kb on + strand, within GFF4736 at 4736.046 kb on - strand, within GFF4736 at 4736.061 kb on - strand, within GFF4736 at 4736.070 kb on - strand, within GFF4736 at 4736.119 kb on - strand, within GFF4736 at 4736.119 kb on - strand, within GFF4736 at 4736.125 kb on + strand, within GFF4736 at 4736.130 kb on + strand, within GFF4736 at 4736.133 kb on - strand, within GFF4736 at 4736.133 kb on - strand, within GFF4736 at 4736.167 kb on + strand, within GFF4736 at 4736.177 kb on + strand, within GFF4736 at 4736.177 kb on + strand, within GFF4736 at 4736.177 kb on + strand, within GFF4736 at 4736.177 kb on + strand, within GFF4736 at 4736.177 kb on + strand, within GFF4736 at 4736.177 kb on + strand, within GFF4736 at 4736.177 kb on + strand, within GFF4736 at 4736.185 kb on - strand, within GFF4736 at 4736.185 kb on - strand, within GFF4736 at 4736.185 kb on - strand, within GFF4736 at 4736.185 kb on - strand, within GFF4736 at 4736.185 kb on - strand, within GFF4736 at 4736.219 kb on + strand, within GFF4736 at 4736.219 kb on - strand, within GFF4736 at 4736.225 kb on - strand, within GFF4736 at 4736.229 kb on - strand, within GFF4736 at 4736.243 kb on + strand, within GFF4736 at 4736.243 kb on + strand, within GFF4736 at 4736.245 kb on - strand, within GFF4736 at 4736.251 kb on - strand, within GFF4736 at 4736.251 kb on - strand, within GFF4736 at 4736.251 kb on - strand, within GFF4736 at 4736.295 kb on - strand, within GFF4736 at 4736.295 kb on - strand, within GFF4736 at 4736.295 kb on - strand, within GFF4736 at 4736.306 kb on - strand, within GFF4736 at 4736.306 kb on - strand, within GFF4736 at 4736.315 kb on + strand, within GFF4736 at 4736.315 kb on + strand, within GFF4736 at 4736.371 kb on + strand, within GFF4736 at 4736.371 kb on + strand, within GFF4736 at 4736.377 kb on - strand, within GFF4736 at 4736.449 kb on - strand at 4736.528 kb on + strand at 4736.538 kb on + strand at 4736.545 kb on + strand at 4736.546 kb on - strand at 4736.722 kb on - strand, within GFF4737 at 4736.806 kb on - strand, within GFF4737 at 4736.822 kb on - strand, within GFF4737 at 4736.956 kb on + strand, within GFF4737 at 4736.967 kb on + strand, within GFF4737 at 4737.008 kb on + strand, within GFF4737 at 4737.018 kb on + strand, within GFF4737 at 4737.020 kb on - strand, within GFF4737 at 4737.032 kb on + strand, within GFF4737 at 4737.037 kb on + strand, within GFF4737 at 4737.040 kb on - strand, within GFF4737 at 4737.040 kb on - strand, within GFF4737 at 4737.063 kb on + strand, within GFF4737 at 4737.063 kb on + strand, within GFF4737 at 4737.063 kb on + strand, within GFF4737 at 4737.079 kb on + strand, within GFF4737 at 4737.079 kb on + strand, within GFF4737 at 4737.079 kb on + strand, within GFF4737 at 4737.094 kb on + strand, within GFF4737 at 4737.096 kb on + strand, within GFF4737 at 4737.102 kb on - strand, within GFF4737 at 4737.124 kb on + strand, within GFF4737 at 4737.133 kb on + strand, within GFF4737 at 4737.133 kb on + strand, within GFF4737 at 4737.141 kb on - strand, within GFF4737 at 4737.142 kb on + strand, within GFF4737 at 4737.143 kb on + strand, within GFF4737 at 4737.156 kb on + strand, within GFF4737 at 4737.158 kb on + strand, within GFF4737 at 4737.158 kb on + strand, within GFF4737 at 4737.166 kb on - strand, within GFF4737 at 4737.166 kb on - strand, within GFF4737 at 4737.168 kb on + strand, within GFF4737 at 4737.175 kb on + strand, within GFF4737 at 4737.190 kb on + strand, within GFF4737 at 4737.208 kb on - strand, within GFF4737 at 4737.218 kb on - strand, within GFF4737 at 4737.253 kb on - strand, within GFF4737 at 4737.291 kb on - strand, within GFF4737 at 4737.447 kb on - strand, within GFF4737 at 4737.462 kb on - strand, within GFF4737 at 4737.462 kb on - strand, within GFF4737 at 4737.462 kb on - strand, within GFF4737 at 4737.474 kb on + strand, within GFF4737 at 4737.488 kb on + strand, within GFF4737 at 4737.545 kb on + strand, within GFF4737 at 4737.568 kb on - strand, within GFF4737 at 4737.569 kb on + strand, within GFF4737 at 4737.615 kb on + strand at 4737.615 kb on + strand at 4737.615 kb on + strand at 4737.623 kb on - strand at 4737.623 kb on - strand at 4737.625 kb on + strand at 4737.626 kb on - strand at 4737.628 kb on - strand at 4737.628 kb on - strand at 4737.633 kb on - strand at 4737.633 kb on - strand at 4737.633 kb on - strand at 4737.633 kb on - strand at 4737.675 kb on + strand at 4737.675 kb on + strand at 4737.675 kb on + strand at 4737.683 kb on - strand at 4737.701 kb on + strand at 4737.727 kb on + strand at 4737.735 kb on - strand at 4737.769 kb on - strand at 4737.774 kb on - strand at 4737.795 kb on + strand at 4737.800 kb on - strand at 4737.803 kb on - strand at 4737.839 kb on - strand at 4737.872 kb on - strand at 4737.905 kb on + strand at 4737.905 kb on + strand at 4737.975 kb on + strand, within GFF4738 at 4737.983 kb on - strand, within GFF4738 at 4738.003 kb on - strand, within GFF4738 at 4738.004 kb on - strand, within GFF4738 at 4738.004 kb on - strand, within GFF4738 at 4738.011 kb on + strand, within GFF4738 at 4738.062 kb on - strand, within GFF4738 at 4738.127 kb on - strand, within GFF4738 at 4738.127 kb on - strand, within GFF4738 at 4738.175 kb on - strand, within GFF4738 at 4738.179 kb on + strand, within GFF4738 at 4738.231 kb on + strand, within GFF4738 at 4738.231 kb on + strand, within GFF4738 at 4738.231 kb on + strand, within GFF4738 at 4738.276 kb on - strand, within GFF4738 at 4738.283 kb on + strand, within GFF4738 at 4738.291 kb on - strand, within GFF4738 at 4738.291 kb on - strand, within GFF4738 at 4738.291 kb on - strand, within GFF4738 at 4738.291 kb on - strand, within GFF4738 at 4738.291 kb on - strand, within GFF4738 at 4738.301 kb on - strand, within GFF4738 at 4738.312 kb on - strand, within GFF4738 at 4738.314 kb on + strand, within GFF4738 at 4738.320 kb on - strand, within GFF4738 at 4738.320 kb on - strand, within GFF4738 at 4738.344 kb on + strand, within GFF4738 at 4738.344 kb on - strand, within GFF4738 at 4738.352 kb on - strand, within GFF4738 at 4738.352 kb on - strand, within GFF4738 at 4738.355 kb on + strand, within GFF4738 at 4738.358 kb on - strand, within GFF4738 at 4738.363 kb on - strand, within GFF4738 at 4738.391 kb on + strand, within GFF4738 at 4738.401 kb on + strand, within GFF4738 at 4738.401 kb on + strand, within GFF4738 at 4738.401 kb on + strand, within GFF4738 at 4738.401 kb on + strand, within GFF4738 at 4738.401 kb on + strand, within GFF4738 at 4738.401 kb on + strand, within GFF4738 at 4738.401 kb on + strand, within GFF4738 at 4738.409 kb on - strand, within GFF4738 at 4738.409 kb on - strand, within GFF4738 at 4738.421 kb on + strand, within GFF4738 at 4738.432 kb on + strand, within GFF4738 at 4738.432 kb on - strand, within GFF4738 at 4738.440 kb on - strand, within GFF4738 at 4738.472 kb on - strand, within GFF4738 at 4738.492 kb on - strand, within GFF4738 at 4738.519 kb on - strand, within GFF4738 at 4738.572 kb on - strand, within GFF4738 at 4738.572 kb on - strand, within GFF4738 at 4738.588 kb on + strand, within GFF4738 at 4738.595 kb on + strand, within GFF4738 at 4738.614 kb on - strand, within GFF4738 at 4738.614 kb on - strand, within GFF4738 at 4738.643 kb on + strand, within GFF4738 at 4738.643 kb on + strand, within GFF4738 at 4738.658 kb on + strand, within GFF4738 at 4738.658 kb on + strand, within GFF4738 at 4738.658 kb on + strand, within GFF4738 at 4738.661 kb on - strand, within GFF4738 at 4738.666 kb on - strand, within GFF4738 at 4738.666 kb on - strand, within GFF4738 at 4738.676 kb on - strand at 4738.686 kb on - strand  
 
 
Per-strain Table 
Position  Strand  Gene  LocusTag  Fraction EMM_noCarbon with D-Glucose and Ammonium Sulfate remove 4,735,591 -  GFF4736 0.16  -0.5 4,735,610 -  GFF4736 0.18  -0.4 4,735,647 +  GFF4736 0.21  -0.3 4,735,657 +  GFF4736 0.22  +0.0 4,735,664 +  GFF4736 0.23  -2.7 4,735,672 -  GFF4736 0.23  +0.6 4,735,677 -  GFF4736 0.24  +0.6 4,735,691 +  GFF4736 0.25  -0.0 4,735,692 +  GFF4736 0.25  +0.1 4,735,705 -  GFF4736 0.26  +0.6 4,735,722 +  GFF4736 0.28  +0.5 4,735,723 -  GFF4736 0.28  +1.1 4,735,727 +  GFF4736 0.28  +0.3 4,735,727 +  GFF4736 0.28  -0.1 4,735,735 -  GFF4736 0.29  +0.8 4,735,759 +  GFF4736 0.31  +1.3 4,735,771 +  GFF4736 0.32  -0.7 4,735,820 +  GFF4736 0.36  -0.3 4,735,828 -  GFF4736 0.37  +0.0 4,735,828 -  GFF4736 0.37  -0.4 4,735,828 -  GFF4736 0.37  -1.7 4,735,828 -  GFF4736 0.37  -1.6 4,735,845 -  GFF4736 0.38  +1.0 4,735,845 -  GFF4736 0.38  -0.7 4,735,845 -  GFF4736 0.38  +1.0 4,735,901 +  GFF4736 0.43  +0.7 4,735,911 +  GFF4736 0.44  +0.3 4,735,928 +  GFF4736 0.45  +0.5 4,735,928 +  GFF4736 0.45  -0.1 4,735,963 +  GFF4736 0.48  +1.1 4,735,963 +  GFF4736 0.48  +0.5 4,735,963 +  GFF4736 0.48  +0.2 4,735,963 +  GFF4736 0.48  -1.0 4,735,976 +  GFF4736 0.49  +2.5 4,735,976 +  GFF4736 0.49  +0.4 4,735,976 +  GFF4736 0.49  +0.1 4,735,976 +  GFF4736 0.49  -0.6 4,735,976 +  GFF4736 0.49  -0.6 4,735,984 -  GFF4736 0.50  -0.3 4,735,984 -  GFF4736 0.50  +0.1 4,735,984 -  GFF4736 0.50  +1.2 4,735,984 -  GFF4736 0.50  +1.5 4,736,027 -  GFF4736 0.54  +0.8 4,736,027 -  GFF4736 0.54  +1.4 4,736,046 +  GFF4736 0.55  +1.4 4,736,046 -  GFF4736 0.55  -0.0 4,736,061 -  GFF4736 0.57  +1.7 4,736,070 -  GFF4736 0.58  +1.0 4,736,119 -  GFF4736 0.62  +1.4 4,736,119 -  GFF4736 0.62  -0.4 4,736,125 +  GFF4736 0.62  -0.5 4,736,130 +  GFF4736 0.63  -0.4 4,736,133 -  GFF4736 0.63  +0.7 4,736,133 -  GFF4736 0.63  +0.6 4,736,167 +  GFF4736 0.66  +0.8 4,736,177 +  GFF4736 0.67  -1.0 4,736,177 +  GFF4736 0.67  -0.5 4,736,177 +  GFF4736 0.67  +0.8 4,736,177 +  GFF4736 0.67  +0.1 4,736,177 +  GFF4736 0.67  +0.8 4,736,177 +  GFF4736 0.67  +0.8 4,736,177 +  GFF4736 0.67  +0.3 4,736,185 -  GFF4736 0.67  -1.0 4,736,185 -  GFF4736 0.67  +0.4 4,736,185 -  GFF4736 0.67  +1.1 4,736,185 -  GFF4736 0.67  +1.1 4,736,185 -  GFF4736 0.67  +0.1 4,736,219 +  GFF4736 0.70  -0.4 4,736,219 -  GFF4736 0.70  +2.3 4,736,225 -  GFF4736 0.71  +1.6 4,736,229 -  GFF4736 0.71  -0.3 4,736,243 +  GFF4736 0.72  -0.1 4,736,243 +  GFF4736 0.72  -0.9 4,736,245 -  GFF4736 0.73  -0.7 4,736,251 -  GFF4736 0.73  +1.7 4,736,251 -  GFF4736 0.73  -0.2 4,736,251 -  GFF4736 0.73  -0.4 4,736,295 -  GFF4736 0.77  +0.1 4,736,295 -  GFF4736 0.77  +1.3 4,736,295 -  GFF4736 0.77  +0.3 4,736,306 -  GFF4736 0.78  +0.1 4,736,306 -  GFF4736 0.78  -0.2 4,736,315 +  GFF4736 0.79  +0.2 4,736,315 +  GFF4736 0.79  -0.9 4,736,371 +  GFF4736 0.83  +0.3 4,736,371 +  GFF4736 0.83  -0.8 4,736,377 -  GFF4736 0.84  -2.0 4,736,449 -  +0.1 4,736,528 +  +0.3 4,736,538 +  +1.1 4,736,545 +  -0.6 4,736,546 -  +0.1 4,736,722 -  GFF4737 0.14  -1.4 4,736,806 -  GFF4737 0.22  +1.1 4,736,822 -  GFF4737 0.23  +0.5 4,736,956 +  GFF4737 0.35  +0.5 4,736,967 +  GFF4737 0.36  +1.8 4,737,008 +  GFF4737 0.40  +1.7 4,737,018 +  GFF4737 0.40  +0.5 4,737,020 -  GFF4737 0.41  +1.4 4,737,032 +  GFF4737 0.42  +0.4 4,737,037 +  GFF4737 0.42  +1.3 4,737,040 -  GFF4737 0.42  -2.6 4,737,040 -  GFF4737 0.42  -1.6 4,737,063 +  GFF4737 0.44  +0.1 4,737,063 +  GFF4737 0.44  +0.6 4,737,063 +  GFF4737 0.44  +2.2 4,737,079 +  GFF4737 0.46  +0.6 4,737,079 +  GFF4737 0.46  -0.7 4,737,079 +  GFF4737 0.46  -0.2 4,737,094 +  GFF4737 0.47  -0.0 4,737,096 +  GFF4737 0.47  -1.6 4,737,102 -  GFF4737 0.48  +0.2 4,737,124 +  GFF4737 0.50  +0.8 4,737,133 +  GFF4737 0.51  +0.9 4,737,133 +  GFF4737 0.51  +0.5 4,737,141 -  GFF4737 0.51  -0.2 4,737,142 +  GFF4737 0.51  -0.6 4,737,143 +  GFF4737 0.52  +0.1 4,737,156 +  GFF4737 0.53  +0.7 4,737,158 +  GFF4737 0.53  +0.9 4,737,158 +  GFF4737 0.53  +0.1 4,737,166 -  GFF4737 0.54  -1.3 4,737,166 -  GFF4737 0.54  -1.7 4,737,168 +  GFF4737 0.54  +0.2 4,737,175 +  GFF4737 0.54  -0.2 4,737,190 +  GFF4737 0.56  -0.3 4,737,208 -  GFF4737 0.57  -0.3 4,737,218 -  GFF4737 0.58  -0.6 4,737,253 -  GFF4737 0.61  +2.0 4,737,291 -  GFF4737 0.65  -0.1 4,737,447 -  GFF4737 0.78  +0.2 4,737,462 -  GFF4737 0.80  +0.8 4,737,462 -  GFF4737 0.80  +0.8 4,737,462 -  GFF4737 0.80  -0.2 4,737,474 +  GFF4737 0.81  +1.2 4,737,488 +  GFF4737 0.82  +0.5 4,737,545 +  GFF4737 0.87  +1.0 4,737,568 -  GFF4737 0.89  +1.4 4,737,569 +  GFF4737 0.89  -1.0 4,737,615 +  -0.2 4,737,615 +  +1.2 4,737,615 +  +0.6 4,737,623 -  -1.2 4,737,623 -  -0.5 4,737,625 +  +0.1 4,737,626 -  +0.1 4,737,628 -  +0.6 4,737,628 -  +1.1 4,737,633 -  +0.1 4,737,633 -  +1.4 4,737,633 -  -1.0 4,737,633 -  -0.3 4,737,675 +  +0.8 4,737,675 +  -0.4 4,737,675 +  +0.7 4,737,683 -  +0.7 4,737,701 +  +0.5 4,737,727 +  -0.4 4,737,735 -  +0.5 4,737,769 -  +1.4 4,737,774 -  -2.4 4,737,795 +  +0.5 4,737,800 -  -1.3 4,737,803 -  +1.3 4,737,839 -  +1.0 4,737,872 -  +1.0 4,737,905 +  +1.0 4,737,905 +  -0.4 4,737,975 +  GFF4738 0.13  -0.1 4,737,983 -  GFF4738 0.14  +1.6 4,738,003 -  GFF4738 0.16  +1.1 4,738,004 -  GFF4738 0.16  +0.8 4,738,004 -  GFF4738 0.16  +0.9 4,738,011 +  GFF4738 0.17  -1.0 4,738,062 -  GFF4738 0.23  -0.7 4,738,127 -  GFF4738 0.30  +0.1 4,738,127 -  GFF4738 0.30  +1.3 4,738,175 -  GFF4738 0.35  -1.0 4,738,179 +  GFF4738 0.36  -0.0 4,738,231 +  GFF4738 0.41  +0.9 4,738,231 +  GFF4738 0.41  -0.4 4,738,231 +  GFF4738 0.41  +0.6 4,738,276 -  GFF4738 0.46  +1.0 4,738,283 +  GFF4738 0.47  -1.5 4,738,291 -  GFF4738 0.48  +1.4 4,738,291 -  GFF4738 0.48  +0.3 4,738,291 -  GFF4738 0.48  -0.1 4,738,291 -  GFF4738 0.48  +1.1 4,738,291 -  GFF4738 0.48  +0.7 4,738,301 -  GFF4738 0.49  -1.0 4,738,312 -  GFF4738 0.50  +2.2 4,738,314 +  GFF4738 0.50  -0.9 4,738,320 -  GFF4738 0.51  +1.1 4,738,320 -  GFF4738 0.51  -1.1 4,738,344 +  GFF4738 0.54  -0.2 4,738,344 -  GFF4738 0.54  -0.0 4,738,352 -  GFF4738 0.55  +0.0 4,738,352 -  GFF4738 0.55  +1.5 4,738,355 +  GFF4738 0.55  +0.1 4,738,358 -  GFF4738 0.55  +1.4 4,738,363 -  GFF4738 0.56  -0.9 4,738,391 +  GFF4738 0.59  -0.4 4,738,401 +  GFF4738 0.60  -2.3 4,738,401 +  GFF4738 0.60  -1.3 4,738,401 +  GFF4738 0.60  -1.1 4,738,401 +  GFF4738 0.60  +0.4 4,738,401 +  GFF4738 0.60  +0.8 4,738,401 +  GFF4738 0.60  +0.4 4,738,401 +  GFF4738 0.60  +0.4 4,738,409 -  GFF4738 0.61  -0.2 4,738,409 -  GFF4738 0.61  +1.0 4,738,421 +  GFF4738 0.62  -0.2 4,738,432 +  GFF4738 0.63  -0.2 4,738,432 -  GFF4738 0.63  +0.8 4,738,440 -  GFF4738 0.64  +0.6 4,738,472 -  GFF4738 0.68  +1.0 4,738,492 -  GFF4738 0.70  +1.3 4,738,519 -  GFF4738 0.73  -0.6 4,738,572 -  GFF4738 0.79  +1.0 4,738,572 -  GFF4738 0.79  +0.9 4,738,588 +  GFF4738 0.80  +0.9 4,738,595 +  GFF4738 0.81  -0.2 4,738,614 -  GFF4738 0.83  +0.7 4,738,614 -  GFF4738 0.83  -0.2 4,738,643 +  GFF4738 0.87  -0.3 4,738,643 +  GFF4738 0.87  -0.7 4,738,658 +  GFF4738 0.88  -0.0 4,738,658 +  GFF4738 0.88  -0.2 4,738,658 +  GFF4738 0.88  +1.4 4,738,661 -  GFF4738 0.88  -0.1 4,738,666 -  GFF4738 0.89  -1.2 4,738,666 -  GFF4738 0.89  -2.2 4,738,676 -  +1.6 4,738,686 -  +1.2 
Or see this region's nucleotide sequence