Experiment: EMM_noCarbon with D-Glucose and Ammonium Sulfate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4162 and GFF4163 are separated by 175 nucleotides GFF4163 and GFF4164 overlap by 8 nucleotides GFF4164 and GFF4165 overlap by 14 nucleotides GFF4165 and GFF4166 are separated by 130 nucleotides GFF4166 and GFF4167 are separated by 71 nucleotides
GFF4162 - Cell wall hydrolyses involved in spore germination, at 4,185,187 to 4,185,858
GFF4162
GFF4163 - hypothetical protein, at 4,186,034 to 4,186,384
GFF4163
GFF4164 - Uncharacterized protein, possibly involved in aromatic compounds catabolism, at 4,186,377 to 4,186,847
GFF4164
GFF4165 - PTS IIA-like nitrogen-regulatory protein PtsN, at 4,186,834 to 4,187,301
GFF4165
GFF4166 - Ribosome hibernation promoting factor Hpf, at 4,187,432 to 4,188,028
GFF4166
GFF4167 - DNA polymerase III epsilon subunit (EC 2.7.7.7), at 4,188,100 to 4,188,801
GFF4167
Position (kb)
4186
4187
4188 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4185.918 kb on + strand at 4185.929 kb on + strand at 4185.929 kb on + strand at 4185.937 kb on - strand at 4185.937 kb on - strand at 4185.980 kb on + strand at 4186.009 kb on - strand at 4186.024 kb on - strand at 4186.060 kb on + strand at 4186.097 kb on - strand, within GFF4163 at 4186.118 kb on - strand, within GFF4163 at 4186.141 kb on + strand, within GFF4163 at 4186.141 kb on + strand, within GFF4163 at 4186.153 kb on + strand, within GFF4163 at 4186.170 kb on + strand, within GFF4163 at 4186.188 kb on - strand, within GFF4163 at 4186.207 kb on - strand, within GFF4163 at 4186.245 kb on + strand, within GFF4163 at 4186.253 kb on - strand, within GFF4163 at 4186.253 kb on - strand, within GFF4163 at 4186.255 kb on + strand, within GFF4163 at 4186.255 kb on + strand, within GFF4163 at 4186.255 kb on + strand, within GFF4163 at 4186.263 kb on - strand, within GFF4163 at 4186.263 kb on - strand, within GFF4163 at 4186.263 kb on - strand, within GFF4163 at 4186.278 kb on + strand, within GFF4163 at 4186.286 kb on - strand, within GFF4163 at 4186.320 kb on + strand, within GFF4163 at 4186.328 kb on - strand, within GFF4163 at 4186.345 kb on - strand, within GFF4163 at 4186.352 kb on + strand at 4186.360 kb on - strand at 4186.360 kb on - strand at 4186.360 kb on - strand at 4186.365 kb on + strand at 4186.373 kb on + strand at 4186.373 kb on + strand at 4186.381 kb on - strand at 4186.394 kb on + strand at 4186.821 kb on - strand at 4186.831 kb on + strand at 4186.890 kb on + strand, within GFF4165 at 4186.890 kb on + strand, within GFF4165 at 4186.898 kb on - strand, within GFF4165 at 4186.925 kb on - strand, within GFF4165 at 4186.965 kb on + strand, within GFF4165 at 4186.973 kb on + strand, within GFF4165 at 4186.981 kb on - strand, within GFF4165 at 4187.150 kb on + strand, within GFF4165 at 4187.189 kb on + strand, within GFF4165 at 4187.202 kb on - strand, within GFF4165 at 4187.202 kb on - strand, within GFF4165 at 4187.272 kb on + strand at 4187.402 kb on + strand at 4187.402 kb on + strand at 4187.414 kb on + strand at 4187.496 kb on + strand, within GFF4166 at 4187.505 kb on + strand, within GFF4166 at 4187.539 kb on + strand, within GFF4166 at 4187.539 kb on + strand, within GFF4166 at 4187.539 kb on + strand, within GFF4166 at 4187.539 kb on + strand, within GFF4166 at 4187.539 kb on + strand, within GFF4166 at 4187.547 kb on - strand, within GFF4166 at 4187.547 kb on - strand, within GFF4166 at 4187.564 kb on - strand, within GFF4166 at 4187.570 kb on + strand, within GFF4166 at 4187.578 kb on - strand, within GFF4166 at 4187.611 kb on - strand, within GFF4166 at 4187.611 kb on - strand, within GFF4166 at 4187.611 kb on - strand, within GFF4166 at 4187.657 kb on + strand, within GFF4166 at 4187.740 kb on + strand, within GFF4166 at 4187.847 kb on - strand, within GFF4166 at 4188.033 kb on - strand at 4188.039 kb on - strand at 4188.093 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with D-Glucose and Ammonium Sulfate remove 4,185,918 + -0.2 4,185,929 + -1.1 4,185,929 + -0.3 4,185,937 - +0.7 4,185,937 - +1.2 4,185,980 + -0.7 4,186,009 - +0.3 4,186,024 - -0.5 4,186,060 + +0.7 4,186,097 - GFF4163 0.18 -0.0 4,186,118 - GFF4163 0.24 +1.1 4,186,141 + GFF4163 0.30 +0.0 4,186,141 + GFF4163 0.30 +0.1 4,186,153 + GFF4163 0.34 +0.4 4,186,170 + GFF4163 0.39 -0.3 4,186,188 - GFF4163 0.44 +0.4 4,186,207 - GFF4163 0.49 +1.4 4,186,245 + GFF4163 0.60 +0.2 4,186,253 - GFF4163 0.62 +0.8 4,186,253 - GFF4163 0.62 +1.0 4,186,255 + GFF4163 0.63 -0.2 4,186,255 + GFF4163 0.63 -0.1 4,186,255 + GFF4163 0.63 -0.6 4,186,263 - GFF4163 0.65 -1.0 4,186,263 - GFF4163 0.65 +0.9 4,186,263 - GFF4163 0.65 -0.1 4,186,278 + GFF4163 0.70 +0.4 4,186,286 - GFF4163 0.72 -0.0 4,186,320 + GFF4163 0.81 -0.8 4,186,328 - GFF4163 0.84 -0.1 4,186,345 - GFF4163 0.89 +0.4 4,186,352 + -0.4 4,186,360 - +0.8 4,186,360 - -2.2 4,186,360 - +1.0 4,186,365 + +1.7 4,186,373 + -0.5 4,186,373 + +0.5 4,186,381 - +0.2 4,186,394 + +1.8 4,186,821 - +0.1 4,186,831 + -0.4 4,186,890 + GFF4165 0.12 -0.9 4,186,890 + GFF4165 0.12 +0.3 4,186,898 - GFF4165 0.14 -1.4 4,186,925 - GFF4165 0.19 -0.8 4,186,965 + GFF4165 0.28 -1.1 4,186,973 + GFF4165 0.30 -0.1 4,186,981 - GFF4165 0.31 -2.4 4,187,150 + GFF4165 0.68 +0.3 4,187,189 + GFF4165 0.76 +0.4 4,187,202 - GFF4165 0.79 -2.1 4,187,202 - GFF4165 0.79 -2.4 4,187,272 + -1.3 4,187,402 + +1.1 4,187,402 + +1.7 4,187,414 + -0.5 4,187,496 + GFF4166 0.11 +1.6 4,187,505 + GFF4166 0.12 +0.7 4,187,539 + GFF4166 0.18 +0.9 4,187,539 + GFF4166 0.18 +0.6 4,187,539 + GFF4166 0.18 +1.1 4,187,539 + GFF4166 0.18 +0.4 4,187,539 + GFF4166 0.18 +1.2 4,187,547 - GFF4166 0.19 +1.6 4,187,547 - GFF4166 0.19 -0.3 4,187,564 - GFF4166 0.22 -0.2 4,187,570 + GFF4166 0.23 +2.4 4,187,578 - GFF4166 0.24 +1.6 4,187,611 - GFF4166 0.30 +1.7 4,187,611 - GFF4166 0.30 +0.9 4,187,611 - GFF4166 0.30 +1.4 4,187,657 + GFF4166 0.38 +0.6 4,187,740 + GFF4166 0.52 -0.7 4,187,847 - GFF4166 0.70 -0.3 4,188,033 - +2.1 4,188,039 - +0.8 4,188,093 + +0.1
Or see this region's nucleotide sequence