Experiment: EMM_noCarbon with D-Glucose and Ammonium Sulfate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3290 and GFF3291 are separated by 33 nucleotides GFF3291 and GFF3292 overlap by 4 nucleotides
GFF3290 - Membrane protein, at 3,343,571 to 3,345,103
GFF3290
GFF3291 - Glycerol-3-phosphate dehydrogenase [NAD(P)+] (EC 1.1.1.94), at 3,345,137 to 3,346,108
GFF3291
GFF3292 - N(6)-L-threonylcarbamoyladenine synthase (EC 2.3.1.234), at 3,346,105 to 3,347,151
GFF3292
Position (kb)
3345
3346
3347 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3344.831 kb on - strand, within GFF3290 at 3345.038 kb on + strand at 3345.038 kb on - strand at 3345.059 kb on - strand at 3345.076 kb on - strand at 3345.082 kb on - strand at 3345.101 kb on + strand at 3345.101 kb on + strand at 3345.104 kb on - strand at 3345.111 kb on - strand at 3345.122 kb on - strand at 3345.122 kb on - strand at 3345.125 kb on - strand at 3345.142 kb on - strand at 3345.158 kb on + strand at 3345.164 kb on - strand at 3345.171 kb on + strand at 3345.171 kb on + strand at 3345.171 kb on + strand at 3345.171 kb on + strand at 3345.171 kb on + strand at 3345.173 kb on + strand at 3345.176 kb on - strand at 3345.179 kb on - strand at 3345.179 kb on - strand at 3345.179 kb on - strand at 3345.198 kb on + strand at 3345.198 kb on + strand at 3345.205 kb on + strand at 3345.206 kb on - strand at 3345.208 kb on + strand at 3345.213 kb on - strand at 3345.216 kb on - strand at 3345.216 kb on - strand at 3345.216 kb on - strand at 3345.216 kb on - strand at 3345.216 kb on - strand at 3345.218 kb on - strand at 3345.218 kb on - strand at 3345.219 kb on + strand at 3345.219 kb on + strand at 3345.219 kb on + strand at 3345.227 kb on - strand at 3345.235 kb on + strand, within GFF3291 at 3345.261 kb on - strand, within GFF3291 at 3345.268 kb on + strand, within GFF3291 at 3345.271 kb on - strand, within GFF3291 at 3345.276 kb on - strand, within GFF3291 at 3345.289 kb on - strand, within GFF3291 at 3345.300 kb on - strand, within GFF3291 at 3345.307 kb on + strand, within GFF3291 at 3345.308 kb on - strand, within GFF3291 at 3345.308 kb on - strand, within GFF3291 at 3345.312 kb on + strand, within GFF3291 at 3345.327 kb on - strand, within GFF3291 at 3345.327 kb on - strand, within GFF3291 at 3345.329 kb on + strand, within GFF3291 at 3345.329 kb on + strand, within GFF3291 at 3345.329 kb on + strand, within GFF3291 at 3345.337 kb on - strand, within GFF3291 at 3345.337 kb on - strand, within GFF3291 at 3345.344 kb on - strand, within GFF3291 at 3345.349 kb on - strand, within GFF3291 at 3345.354 kb on - strand, within GFF3291 at 3345.358 kb on + strand, within GFF3291 at 3345.373 kb on + strand, within GFF3291 at 3345.384 kb on + strand, within GFF3291 at 3345.390 kb on + strand, within GFF3291 at 3345.397 kb on - strand, within GFF3291 at 3345.399 kb on + strand, within GFF3291 at 3345.404 kb on + strand, within GFF3291 at 3345.409 kb on + strand, within GFF3291 at 3345.412 kb on - strand, within GFF3291 at 3345.416 kb on - strand, within GFF3291 at 3345.479 kb on + strand, within GFF3291 at 3345.479 kb on + strand, within GFF3291 at 3345.479 kb on + strand, within GFF3291 at 3345.505 kb on + strand, within GFF3291 at 3345.505 kb on + strand, within GFF3291 at 3345.505 kb on + strand, within GFF3291 at 3345.513 kb on - strand, within GFF3291 at 3345.513 kb on - strand, within GFF3291 at 3345.513 kb on - strand, within GFF3291 at 3345.517 kb on + strand, within GFF3291 at 3345.533 kb on - strand, within GFF3291 at 3345.537 kb on + strand, within GFF3291 at 3345.545 kb on - strand, within GFF3291 at 3345.565 kb on - strand, within GFF3291 at 3345.566 kb on - strand, within GFF3291 at 3345.585 kb on - strand, within GFF3291 at 3345.612 kb on - strand, within GFF3291 at 3345.669 kb on - strand, within GFF3291 at 3345.671 kb on + strand, within GFF3291 at 3345.671 kb on + strand, within GFF3291 at 3345.671 kb on + strand, within GFF3291 at 3345.677 kb on + strand, within GFF3291 at 3345.679 kb on - strand, within GFF3291 at 3345.679 kb on - strand, within GFF3291 at 3345.685 kb on - strand, within GFF3291 at 3345.689 kb on + strand, within GFF3291 at 3345.704 kb on - strand, within GFF3291 at 3345.706 kb on + strand, within GFF3291 at 3345.714 kb on - strand, within GFF3291 at 3345.717 kb on + strand, within GFF3291 at 3345.719 kb on + strand, within GFF3291 at 3345.719 kb on + strand, within GFF3291 at 3345.779 kb on + strand, within GFF3291 at 3345.782 kb on + strand, within GFF3291 at 3345.782 kb on + strand, within GFF3291 at 3345.782 kb on + strand, within GFF3291 at 3345.787 kb on - strand, within GFF3291 at 3345.790 kb on - strand, within GFF3291 at 3345.790 kb on - strand, within GFF3291 at 3345.790 kb on - strand, within GFF3291 at 3345.790 kb on - strand, within GFF3291 at 3345.792 kb on + strand, within GFF3291 at 3345.795 kb on - strand, within GFF3291 at 3345.799 kb on + strand, within GFF3291 at 3345.799 kb on + strand, within GFF3291 at 3345.800 kb on - strand, within GFF3291 at 3345.800 kb on - strand, within GFF3291 at 3345.816 kb on - strand, within GFF3291 at 3345.816 kb on - strand, within GFF3291 at 3345.816 kb on - strand, within GFF3291 at 3345.816 kb on - strand, within GFF3291 at 3345.863 kb on - strand, within GFF3291 at 3345.874 kb on - strand, within GFF3291 at 3345.875 kb on - strand, within GFF3291 at 3345.884 kb on - strand, within GFF3291 at 3345.893 kb on - strand, within GFF3291 at 3345.903 kb on - strand, within GFF3291 at 3345.919 kb on - strand, within GFF3291 at 3345.919 kb on - strand, within GFF3291 at 3345.927 kb on - strand, within GFF3291 at 3345.935 kb on + strand, within GFF3291 at 3345.935 kb on + strand, within GFF3291 at 3345.939 kb on - strand, within GFF3291 at 3345.960 kb on + strand, within GFF3291 at 3346.000 kb on - strand, within GFF3291 at 3346.006 kb on + strand, within GFF3291 at 3346.020 kb on + strand at 3346.020 kb on + strand at 3346.020 kb on + strand at 3346.028 kb on - strand at 3346.028 kb on - strand at 3346.033 kb on + strand at 3346.060 kb on + strand at 3346.084 kb on - strand at 3346.084 kb on - strand at 3346.116 kb on + strand at 3346.116 kb on + strand at 3346.118 kb on - strand at 3346.118 kb on - strand at 3346.118 kb on - strand at 3346.124 kb on - strand at 3346.124 kb on - strand at 3346.124 kb on - strand at 3346.124 kb on - strand at 3346.124 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with D-Glucose and Ammonium Sulfate remove 3,344,831 - GFF3290 0.82 -1.5 3,345,038 + -1.4 3,345,038 - +0.3 3,345,059 - +0.8 3,345,076 - -0.5 3,345,082 - -1.9 3,345,101 + +1.0 3,345,101 + -1.7 3,345,104 - +0.8 3,345,111 - -1.9 3,345,122 - -1.5 3,345,122 - +0.4 3,345,125 - -0.0 3,345,142 - -1.4 3,345,158 + -1.4 3,345,164 - +0.8 3,345,171 + -1.2 3,345,171 + -2.2 3,345,171 + -0.6 3,345,171 + -2.2 3,345,171 + +0.1 3,345,173 + -0.9 3,345,176 - -0.4 3,345,179 - -0.0 3,345,179 - -0.4 3,345,179 - -0.4 3,345,198 + -1.0 3,345,198 + +0.3 3,345,205 + -0.4 3,345,206 - +0.4 3,345,208 + -0.3 3,345,213 - -1.8 3,345,216 - -2.3 3,345,216 - +0.6 3,345,216 - -0.3 3,345,216 - -2.1 3,345,216 - -1.4 3,345,218 - -0.3 3,345,218 - +0.0 3,345,219 + -0.4 3,345,219 + +0.5 3,345,219 + -0.4 3,345,227 - -2.2 3,345,235 + GFF3291 0.10 -3.3 3,345,261 - GFF3291 0.13 -2.0 3,345,268 + GFF3291 0.13 +0.2 3,345,271 - GFF3291 0.14 -0.7 3,345,276 - GFF3291 0.14 -2.9 3,345,289 - GFF3291 0.16 -1.9 3,345,300 - GFF3291 0.17 -1.5 3,345,307 + GFF3291 0.17 -1.6 3,345,308 - GFF3291 0.18 -2.4 3,345,308 - GFF3291 0.18 -1.0 3,345,312 + GFF3291 0.18 -0.9 3,345,327 - GFF3291 0.20 +0.1 3,345,327 - GFF3291 0.20 +0.8 3,345,329 + GFF3291 0.20 +0.3 3,345,329 + GFF3291 0.20 -0.2 3,345,329 + GFF3291 0.20 -1.7 3,345,337 - GFF3291 0.21 -0.1 3,345,337 - GFF3291 0.21 -1.0 3,345,344 - GFF3291 0.21 +0.8 3,345,349 - GFF3291 0.22 -0.9 3,345,354 - GFF3291 0.22 -0.5 3,345,358 + GFF3291 0.23 -0.2 3,345,373 + GFF3291 0.24 -0.1 3,345,384 + GFF3291 0.25 -0.0 3,345,390 + GFF3291 0.26 -0.4 3,345,397 - GFF3291 0.27 -2.0 3,345,399 + GFF3291 0.27 -1.3 3,345,404 + GFF3291 0.27 -1.8 3,345,409 + GFF3291 0.28 -1.7 3,345,412 - GFF3291 0.28 +1.0 3,345,416 - GFF3291 0.29 -1.6 3,345,479 + GFF3291 0.35 -2.0 3,345,479 + GFF3291 0.35 -0.5 3,345,479 + GFF3291 0.35 +0.1 3,345,505 + GFF3291 0.38 -2.2 3,345,505 + GFF3291 0.38 +0.4 3,345,505 + GFF3291 0.38 -0.1 3,345,513 - GFF3291 0.39 -2.5 3,345,513 - GFF3291 0.39 -0.0 3,345,513 - GFF3291 0.39 -1.2 3,345,517 + GFF3291 0.39 -2.5 3,345,533 - GFF3291 0.41 -1.3 3,345,537 + GFF3291 0.41 +0.0 3,345,545 - GFF3291 0.42 -1.5 3,345,565 - GFF3291 0.44 -2.7 3,345,566 - GFF3291 0.44 -1.7 3,345,585 - GFF3291 0.46 -1.6 3,345,612 - GFF3291 0.49 -0.8 3,345,669 - GFF3291 0.55 +1.0 3,345,671 + GFF3291 0.55 -1.4 3,345,671 + GFF3291 0.55 -1.1 3,345,671 + GFF3291 0.55 -0.2 3,345,677 + GFF3291 0.56 -1.9 3,345,679 - GFF3291 0.56 -0.4 3,345,679 - GFF3291 0.56 -0.7 3,345,685 - GFF3291 0.56 +1.1 3,345,689 + GFF3291 0.57 -0.2 3,345,704 - GFF3291 0.58 -1.7 3,345,706 + GFF3291 0.59 -0.6 3,345,714 - GFF3291 0.59 -1.6 3,345,717 + GFF3291 0.60 -0.1 3,345,719 + GFF3291 0.60 -1.2 3,345,719 + GFF3291 0.60 -0.7 3,345,779 + GFF3291 0.66 -0.8 3,345,782 + GFF3291 0.66 -2.1 3,345,782 + GFF3291 0.66 -2.5 3,345,782 + GFF3291 0.66 -0.3 3,345,787 - GFF3291 0.67 -1.4 3,345,790 - GFF3291 0.67 -0.6 3,345,790 - GFF3291 0.67 -0.4 3,345,790 - GFF3291 0.67 -1.6 3,345,790 - GFF3291 0.67 +0.5 3,345,792 + GFF3291 0.67 -1.9 3,345,795 - GFF3291 0.68 -0.8 3,345,799 + GFF3291 0.68 +0.1 3,345,799 + GFF3291 0.68 -0.3 3,345,800 - GFF3291 0.68 -1.4 3,345,800 - GFF3291 0.68 -0.5 3,345,816 - GFF3291 0.70 -2.1 3,345,816 - GFF3291 0.70 -0.1 3,345,816 - GFF3291 0.70 -1.8 3,345,816 - GFF3291 0.70 -1.0 3,345,863 - GFF3291 0.75 +0.1 3,345,874 - GFF3291 0.76 -2.1 3,345,875 - GFF3291 0.76 -0.6 3,345,884 - GFF3291 0.77 -1.8 3,345,893 - GFF3291 0.78 +0.6 3,345,903 - GFF3291 0.79 -1.4 3,345,919 - GFF3291 0.80 -0.4 3,345,919 - GFF3291 0.80 -1.3 3,345,927 - GFF3291 0.81 -1.9 3,345,935 + GFF3291 0.82 +0.5 3,345,935 + GFF3291 0.82 -2.1 3,345,939 - GFF3291 0.83 -1.5 3,345,960 + GFF3291 0.85 -1.6 3,346,000 - GFF3291 0.89 -1.1 3,346,006 + GFF3291 0.89 -1.9 3,346,020 + -1.0 3,346,020 + -0.5 3,346,020 + +0.2 3,346,028 - +0.1 3,346,028 - -1.4 3,346,033 + -1.4 3,346,060 + -2.1 3,346,084 - -0.4 3,346,084 - -1.2 3,346,116 + -0.7 3,346,116 + -0.0 3,346,118 - +0.2 3,346,118 - +0.6 3,346,118 - -0.2 3,346,124 - +0.8 3,346,124 - +0.4 3,346,124 - -1.8 3,346,124 - -0.5 3,346,124 - -0.3
Or see this region's nucleotide sequence