Experiment: EMM_noCarbon with D-Glucose and Ammonium Sulfate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2546 and GFF2547 are separated by 145 nucleotides GFF2547 and GFF2548 are separated by 15 nucleotides GFF2548 and GFF2549 overlap by 4 nucleotides
GFF2546 - Fatty acid desaturase, type 2, at 2,564,078 to 2,565,145
GFF2546
GFF2547 - Hypothetical protein associated with Serine palmitoyltransferase, at 2,565,291 to 2,566,448
GFF2547
GFF2548 - hypothetical protein, at 2,566,464 to 2,567,279
GFF2548
GFF2549 - TonB-dependent receptor, at 2,567,276 to 2,569,498
GFF2549
Position (kb)
2565
2566
2567 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2564.452 kb on - strand, within GFF2546 at 2564.697 kb on + strand, within GFF2546 at 2564.705 kb on - strand, within GFF2546 at 2565.020 kb on - strand, within GFF2546 at 2565.089 kb on + strand at 2565.089 kb on + strand at 2565.097 kb on - strand at 2565.099 kb on - strand at 2565.141 kb on + strand at 2565.143 kb on + strand at 2565.149 kb on + strand at 2565.149 kb on - strand at 2565.149 kb on - strand at 2565.180 kb on - strand at 2565.206 kb on - strand at 2566.443 kb on + strand at 2566.445 kb on + strand at 2566.451 kb on - strand at 2566.451 kb on - strand at 2566.455 kb on + strand at 2566.457 kb on - strand at 2566.465 kb on - strand at 2566.477 kb on - strand at 2566.513 kb on + strand at 2566.535 kb on - strand at 2566.537 kb on + strand at 2566.568 kb on + strand, within GFF2548 at 2566.568 kb on + strand, within GFF2548 at 2566.576 kb on - strand, within GFF2548 at 2566.586 kb on + strand, within GFF2548 at 2566.594 kb on - strand, within GFF2548 at 2566.594 kb on - strand, within GFF2548 at 2566.597 kb on + strand, within GFF2548 at 2566.597 kb on + strand, within GFF2548 at 2566.600 kb on - strand, within GFF2548 at 2566.602 kb on + strand, within GFF2548 at 2566.602 kb on + strand, within GFF2548 at 2566.602 kb on + strand, within GFF2548 at 2566.607 kb on + strand, within GFF2548 at 2566.610 kb on - strand, within GFF2548 at 2566.610 kb on - strand, within GFF2548 at 2566.610 kb on - strand, within GFF2548 at 2566.610 kb on - strand, within GFF2548 at 2566.610 kb on - strand, within GFF2548 at 2566.612 kb on + strand, within GFF2548 at 2566.615 kb on - strand, within GFF2548 at 2566.615 kb on - strand, within GFF2548 at 2566.620 kb on - strand, within GFF2548 at 2566.635 kb on - strand, within GFF2548 at 2566.643 kb on + strand, within GFF2548 at 2566.643 kb on + strand, within GFF2548 at 2566.651 kb on - strand, within GFF2548 at 2566.668 kb on - strand, within GFF2548 at 2566.694 kb on + strand, within GFF2548 at 2566.699 kb on + strand, within GFF2548 at 2566.702 kb on - strand, within GFF2548 at 2566.712 kb on + strand, within GFF2548 at 2566.715 kb on - strand, within GFF2548 at 2566.715 kb on - strand, within GFF2548 at 2566.720 kb on - strand, within GFF2548 at 2566.720 kb on - strand, within GFF2548 at 2566.720 kb on - strand, within GFF2548 at 2566.720 kb on - strand, within GFF2548 at 2566.722 kb on + strand, within GFF2548 at 2566.722 kb on + strand, within GFF2548 at 2566.722 kb on + strand, within GFF2548 at 2566.730 kb on - strand, within GFF2548 at 2566.732 kb on + strand, within GFF2548 at 2566.750 kb on + strand, within GFF2548 at 2566.760 kb on + strand, within GFF2548 at 2566.760 kb on + strand, within GFF2548 at 2566.763 kb on - strand, within GFF2548 at 2566.763 kb on - strand, within GFF2548 at 2566.794 kb on - strand, within GFF2548 at 2566.804 kb on + strand, within GFF2548 at 2566.804 kb on + strand, within GFF2548 at 2566.816 kb on + strand, within GFF2548 at 2566.833 kb on - strand, within GFF2548 at 2566.835 kb on + strand, within GFF2548 at 2566.838 kb on - strand, within GFF2548 at 2566.843 kb on - strand, within GFF2548 at 2566.849 kb on + strand, within GFF2548 at 2566.849 kb on + strand, within GFF2548 at 2566.849 kb on + strand, within GFF2548 at 2566.854 kb on + strand, within GFF2548 at 2566.857 kb on - strand, within GFF2548 at 2566.859 kb on + strand, within GFF2548 at 2566.870 kb on + strand, within GFF2548 at 2566.878 kb on - strand, within GFF2548 at 2566.878 kb on - strand, within GFF2548 at 2566.880 kb on + strand, within GFF2548 at 2566.880 kb on + strand, within GFF2548 at 2566.880 kb on + strand, within GFF2548 at 2566.880 kb on + strand, within GFF2548 at 2566.880 kb on + strand, within GFF2548 at 2566.881 kb on - strand, within GFF2548 at 2566.936 kb on + strand, within GFF2548 at 2566.936 kb on - strand, within GFF2548 at 2566.936 kb on - strand, within GFF2548 at 2566.941 kb on + strand, within GFF2548 at 2566.989 kb on + strand, within GFF2548 at 2566.989 kb on + strand, within GFF2548 at 2566.989 kb on + strand, within GFF2548 at 2566.989 kb on + strand, within GFF2548 at 2566.994 kb on + strand, within GFF2548 at 2566.997 kb on - strand, within GFF2548 at 2566.997 kb on - strand, within GFF2548 at 2566.997 kb on - strand, within GFF2548 at 2566.997 kb on - strand, within GFF2548 at 2567.002 kb on - strand, within GFF2548 at 2567.002 kb on - strand, within GFF2548 at 2567.017 kb on - strand, within GFF2548 at 2567.017 kb on - strand, within GFF2548 at 2567.029 kb on + strand, within GFF2548 at 2567.029 kb on + strand, within GFF2548 at 2567.034 kb on + strand, within GFF2548 at 2567.036 kb on - strand, within GFF2548 at 2567.037 kb on - strand, within GFF2548 at 2567.043 kb on + strand, within GFF2548 at 2567.045 kb on + strand, within GFF2548 at 2567.045 kb on - strand, within GFF2548 at 2567.051 kb on - strand, within GFF2548 at 2567.053 kb on + strand, within GFF2548 at 2567.053 kb on + strand, within GFF2548 at 2567.053 kb on - strand, within GFF2548 at 2567.056 kb on - strand, within GFF2548 at 2567.061 kb on - strand, within GFF2548 at 2567.089 kb on + strand, within GFF2548 at 2567.102 kb on - strand, within GFF2548 at 2567.116 kb on - strand, within GFF2548 at 2567.125 kb on + strand, within GFF2548 at 2567.144 kb on - strand, within GFF2548 at 2567.149 kb on - strand, within GFF2548 at 2567.167 kb on + strand, within GFF2548 at 2567.167 kb on + strand, within GFF2548 at 2567.175 kb on - strand, within GFF2548 at 2567.175 kb on - strand, within GFF2548 at 2567.186 kb on + strand, within GFF2548 at 2567.194 kb on - strand, within GFF2548 at 2567.194 kb on - strand, within GFF2548 at 2567.233 kb on + strand at 2567.241 kb on - strand at 2567.241 kb on - strand at 2567.241 kb on - strand at 2567.248 kb on + strand at 2567.248 kb on + strand at 2567.248 kb on + strand at 2567.248 kb on + strand at 2567.252 kb on + strand at 2567.283 kb on + strand at 2567.291 kb on - strand at 2567.326 kb on + strand at 2567.326 kb on + strand at 2567.328 kb on + strand at 2567.334 kb on - strand at 2567.334 kb on - strand at 2567.334 kb on - strand at 2567.334 kb on - strand at 2567.341 kb on - strand at 2567.341 kb on - strand at 2567.341 kb on - strand at 2567.341 kb on - strand at 2567.360 kb on + strand at 2567.368 kb on - strand at 2567.378 kb on - strand at 2567.385 kb on + strand at 2567.417 kb on + strand at 2567.417 kb on + strand at 2567.442 kb on + strand at 2567.442 kb on + strand at 2567.442 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with D-Glucose and Ammonium Sulfate remove 2,564,452 - GFF2546 0.35 +0.8 2,564,697 + GFF2546 0.58 -1.4 2,564,705 - GFF2546 0.59 +1.8 2,565,020 - GFF2546 0.88 -1.5 2,565,089 + -1.9 2,565,089 + -0.7 2,565,097 - -2.6 2,565,099 - -1.2 2,565,141 + +0.2 2,565,143 + +0.9 2,565,149 + -0.2 2,565,149 - -0.2 2,565,149 - -0.1 2,565,180 - -1.2 2,565,206 - +0.8 2,566,443 + +1.2 2,566,445 + -1.2 2,566,451 - -2.0 2,566,451 - -0.4 2,566,455 + +1.1 2,566,457 - -1.8 2,566,465 - -1.5 2,566,477 - -2.2 2,566,513 + -0.4 2,566,535 - -0.1 2,566,537 + -1.0 2,566,568 + GFF2548 0.13 -0.2 2,566,568 + GFF2548 0.13 +1.0 2,566,576 - GFF2548 0.14 -1.1 2,566,586 + GFF2548 0.15 +0.8 2,566,594 - GFF2548 0.16 +0.0 2,566,594 - GFF2548 0.16 +1.0 2,566,597 + GFF2548 0.16 -1.1 2,566,597 + GFF2548 0.16 -1.3 2,566,600 - GFF2548 0.17 -0.7 2,566,602 + GFF2548 0.17 -0.1 2,566,602 + GFF2548 0.17 +0.9 2,566,602 + GFF2548 0.17 -0.0 2,566,607 + GFF2548 0.18 -0.9 2,566,610 - GFF2548 0.18 -0.4 2,566,610 - GFF2548 0.18 -0.6 2,566,610 - GFF2548 0.18 -0.4 2,566,610 - GFF2548 0.18 +0.3 2,566,610 - GFF2548 0.18 +0.0 2,566,612 + GFF2548 0.18 +0.5 2,566,615 - GFF2548 0.19 -0.9 2,566,615 - GFF2548 0.19 +0.6 2,566,620 - GFF2548 0.19 -1.3 2,566,635 - GFF2548 0.21 +1.6 2,566,643 + GFF2548 0.22 +0.1 2,566,643 + GFF2548 0.22 -0.4 2,566,651 - GFF2548 0.23 -0.6 2,566,668 - GFF2548 0.25 -1.9 2,566,694 + GFF2548 0.28 -0.4 2,566,699 + GFF2548 0.29 -0.9 2,566,702 - GFF2548 0.29 -1.0 2,566,712 + GFF2548 0.30 -0.4 2,566,715 - GFF2548 0.31 -0.1 2,566,715 - GFF2548 0.31 +1.4 2,566,720 - GFF2548 0.31 -0.2 2,566,720 - GFF2548 0.31 +0.9 2,566,720 - GFF2548 0.31 -1.0 2,566,720 - GFF2548 0.31 +0.5 2,566,722 + GFF2548 0.32 -0.2 2,566,722 + GFF2548 0.32 -1.9 2,566,722 + GFF2548 0.32 -0.4 2,566,730 - GFF2548 0.33 +0.9 2,566,732 + GFF2548 0.33 +0.6 2,566,750 + GFF2548 0.35 +0.1 2,566,760 + GFF2548 0.36 -1.0 2,566,760 + GFF2548 0.36 +0.2 2,566,763 - GFF2548 0.37 -0.3 2,566,763 - GFF2548 0.37 -1.7 2,566,794 - GFF2548 0.40 +0.6 2,566,804 + GFF2548 0.42 +0.0 2,566,804 + GFF2548 0.42 -0.1 2,566,816 + GFF2548 0.43 +0.8 2,566,833 - GFF2548 0.45 -0.5 2,566,835 + GFF2548 0.45 -0.0 2,566,838 - GFF2548 0.46 -1.6 2,566,843 - GFF2548 0.46 +1.1 2,566,849 + GFF2548 0.47 -0.2 2,566,849 + GFF2548 0.47 -0.2 2,566,849 + GFF2548 0.47 +0.4 2,566,854 + GFF2548 0.48 -1.5 2,566,857 - GFF2548 0.48 -0.2 2,566,859 + GFF2548 0.48 -1.2 2,566,870 + GFF2548 0.50 -1.0 2,566,878 - GFF2548 0.51 -0.3 2,566,878 - GFF2548 0.51 -0.1 2,566,880 + GFF2548 0.51 +0.2 2,566,880 + GFF2548 0.51 +0.2 2,566,880 + GFF2548 0.51 +1.2 2,566,880 + GFF2548 0.51 -1.5 2,566,880 + GFF2548 0.51 +0.7 2,566,881 - GFF2548 0.51 -0.9 2,566,936 + GFF2548 0.58 -0.6 2,566,936 - GFF2548 0.58 -1.7 2,566,936 - GFF2548 0.58 -0.1 2,566,941 + GFF2548 0.58 -0.3 2,566,989 + GFF2548 0.64 -0.2 2,566,989 + GFF2548 0.64 +0.4 2,566,989 + GFF2548 0.64 +0.5 2,566,989 + GFF2548 0.64 -0.1 2,566,994 + GFF2548 0.65 -0.4 2,566,997 - GFF2548 0.65 +0.3 2,566,997 - GFF2548 0.65 -0.4 2,566,997 - GFF2548 0.65 -1.1 2,566,997 - GFF2548 0.65 -0.5 2,567,002 - GFF2548 0.66 +1.7 2,567,002 - GFF2548 0.66 -0.2 2,567,017 - GFF2548 0.68 +0.1 2,567,017 - GFF2548 0.68 -0.1 2,567,029 + GFF2548 0.69 -1.0 2,567,029 + GFF2548 0.69 +0.5 2,567,034 + GFF2548 0.70 -0.0 2,567,036 - GFF2548 0.70 +0.4 2,567,037 - GFF2548 0.70 +0.1 2,567,043 + GFF2548 0.71 +1.5 2,567,045 + GFF2548 0.71 -0.0 2,567,045 - GFF2548 0.71 +0.2 2,567,051 - GFF2548 0.72 -1.6 2,567,053 + GFF2548 0.72 +0.8 2,567,053 + GFF2548 0.72 -1.8 2,567,053 - GFF2548 0.72 -0.5 2,567,056 - GFF2548 0.73 -2.1 2,567,061 - GFF2548 0.73 -1.3 2,567,089 + GFF2548 0.77 +0.6 2,567,102 - GFF2548 0.78 +0.4 2,567,116 - GFF2548 0.80 -2.3 2,567,125 + GFF2548 0.81 -0.1 2,567,144 - GFF2548 0.83 -0.5 2,567,149 - GFF2548 0.84 -1.9 2,567,167 + GFF2548 0.86 +0.2 2,567,167 + GFF2548 0.86 +0.4 2,567,175 - GFF2548 0.87 -0.1 2,567,175 - GFF2548 0.87 -1.1 2,567,186 + GFF2548 0.88 -0.8 2,567,194 - GFF2548 0.89 +0.3 2,567,194 - GFF2548 0.89 -1.4 2,567,233 + +0.1 2,567,241 - -0.5 2,567,241 - -1.2 2,567,241 - -1.4 2,567,248 + -1.1 2,567,248 + +0.4 2,567,248 + -1.5 2,567,248 + -0.0 2,567,252 + -1.5 2,567,283 + -1.2 2,567,291 - -0.1 2,567,326 + +0.3 2,567,326 + +0.6 2,567,328 + -1.8 2,567,334 - +1.3 2,567,334 - -1.0 2,567,334 - -2.1 2,567,334 - -1.7 2,567,341 - +0.1 2,567,341 - +0.3 2,567,341 - -0.8 2,567,341 - +0.9 2,567,360 + +0.3 2,567,368 - -1.0 2,567,378 - +0.1 2,567,385 + +0.1 2,567,417 + +0.3 2,567,417 + -0.7 2,567,442 + -1.2 2,567,442 + +0.4 2,567,442 + +1.2
Or see this region's nucleotide sequence