Experiment: EMM_noCarbon with D-Glucose and Ammonium Sulfate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2488 and GFF2489 are separated by 38 nucleotides GFF2489 and GFF2490 are separated by 81 nucleotides GFF2490 and GFF2491 are separated by 62 nucleotides GFF2491 and GFF2492 are separated by 23 nucleotides GFF2492 and GFF2493 are separated by 42 nucleotides GFF2493 and GFF2494 are separated by 40 nucleotides
GFF2488 - Periplasmic aromatic amino acid aminotransferase beta precursor (EC 2.6.1.57), at 2,510,388 to 2,511,545
GFF2488
GFF2489 - hypothetical protein, at 2,511,584 to 2,511,829
GFF2489
GFF2490 - asparaginase/glutaminase, at 2,511,911 to 2,512,414
GFF2490
GFF2491 - Pyrroline-5-carboxylate reductase (EC 1.5.1.2), at 2,512,477 to 2,513,292
GFF2491
GFF2492 - Uracil DNA glycosylase, at 2,513,316 to 2,513,822
GFF2492
GFF2493 - FIG018229: hypothetical protein, at 2,513,865 to 2,514,176
GFF2493
GFF2494 - TspO and MBR like proteins, at 2,514,217 to 2,514,765
GFF2494
Position (kb)
2512
2513
2514 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2511.478 kb on - strand at 2511.504 kb on + strand at 2511.511 kb on - strand at 2511.518 kb on - strand at 2511.520 kb on - strand at 2511.520 kb on - strand at 2511.532 kb on + strand at 2511.532 kb on + strand at 2511.532 kb on + strand at 2511.535 kb on - strand at 2511.540 kb on - strand at 2511.547 kb on - strand at 2511.559 kb on + strand at 2511.564 kb on + strand at 2511.564 kb on + strand at 2511.577 kb on + strand at 2511.590 kb on + strand at 2511.600 kb on - strand at 2511.606 kb on + strand at 2511.610 kb on - strand, within GFF2489 at 2511.619 kb on - strand, within GFF2489 at 2511.621 kb on - strand, within GFF2489 at 2511.631 kb on - strand, within GFF2489 at 2511.636 kb on + strand, within GFF2489 at 2511.729 kb on + strand, within GFF2489 at 2511.755 kb on + strand, within GFF2489 at 2511.833 kb on - strand at 2511.844 kb on + strand at 2511.857 kb on - strand at 2511.885 kb on + strand at 2511.887 kb on - strand at 2511.925 kb on + strand at 2511.925 kb on + strand at 2511.936 kb on + strand at 2511.952 kb on + strand at 2512.008 kb on + strand, within GFF2490 at 2512.008 kb on + strand, within GFF2490 at 2512.014 kb on - strand, within GFF2490 at 2512.025 kb on + strand, within GFF2490 at 2512.033 kb on - strand, within GFF2490 at 2512.033 kb on - strand, within GFF2490 at 2512.033 kb on - strand, within GFF2490 at 2512.033 kb on - strand, within GFF2490 at 2512.033 kb on - strand, within GFF2490 at 2512.033 kb on - strand, within GFF2490 at 2512.043 kb on + strand, within GFF2490 at 2512.050 kb on - strand, within GFF2490 at 2512.058 kb on + strand, within GFF2490 at 2512.063 kb on + strand, within GFF2490 at 2512.071 kb on - strand, within GFF2490 at 2512.074 kb on + strand, within GFF2490 at 2512.096 kb on + strand, within GFF2490 at 2512.104 kb on - strand, within GFF2490 at 2512.159 kb on - strand, within GFF2490 at 2512.234 kb on + strand, within GFF2490 at 2512.234 kb on + strand, within GFF2490 at 2512.234 kb on + strand, within GFF2490 at 2512.234 kb on + strand, within GFF2490 at 2512.234 kb on + strand, within GFF2490 at 2512.234 kb on + strand, within GFF2490 at 2512.242 kb on - strand, within GFF2490 at 2512.242 kb on - strand, within GFF2490 at 2512.244 kb on + strand, within GFF2490 at 2512.261 kb on - strand, within GFF2490 at 2512.278 kb on + strand, within GFF2490 at 2512.278 kb on + strand, within GFF2490 at 2512.278 kb on + strand, within GFF2490 at 2512.278 kb on + strand, within GFF2490 at 2512.286 kb on - strand, within GFF2490 at 2512.286 kb on - strand, within GFF2490 at 2512.286 kb on - strand, within GFF2490 at 2512.293 kb on + strand, within GFF2490 at 2512.293 kb on + strand, within GFF2490 at 2512.293 kb on + strand, within GFF2490 at 2512.301 kb on - strand, within GFF2490 at 2512.301 kb on - strand, within GFF2490 at 2512.303 kb on + strand, within GFF2490 at 2512.303 kb on - strand, within GFF2490 at 2512.311 kb on - strand, within GFF2490 at 2512.340 kb on - strand, within GFF2490 at 2512.350 kb on + strand, within GFF2490 at 2512.358 kb on - strand, within GFF2490 at 2512.390 kb on + strand at 2512.390 kb on + strand at 2512.390 kb on + strand at 2512.390 kb on + strand at 2512.390 kb on + strand at 2512.398 kb on - strand at 2512.398 kb on - strand at 2512.398 kb on - strand at 2512.398 kb on - strand at 2512.433 kb on - strand at 2512.451 kb on - strand at 2512.463 kb on + strand at 2512.463 kb on + strand at 2512.463 kb on + strand at 2512.463 kb on + strand at 2512.465 kb on + strand at 2512.471 kb on - strand at 2512.471 kb on - strand at 2512.471 kb on - strand at 2512.471 kb on - strand at 2512.471 kb on - strand at 2512.471 kb on - strand at 2512.531 kb on + strand at 2512.532 kb on - strand at 2512.569 kb on - strand, within GFF2491 at 2512.577 kb on - strand, within GFF2491 at 2512.577 kb on - strand, within GFF2491 at 2512.663 kb on + strand, within GFF2491 at 2512.663 kb on + strand, within GFF2491 at 2512.670 kb on + strand, within GFF2491 at 2512.671 kb on - strand, within GFF2491 at 2512.671 kb on - strand, within GFF2491 at 2512.671 kb on - strand, within GFF2491 at 2512.701 kb on - strand, within GFF2491 at 2512.719 kb on - strand, within GFF2491 at 2512.719 kb on - strand, within GFF2491 at 2512.762 kb on + strand, within GFF2491 at 2512.774 kb on + strand, within GFF2491 at 2512.780 kb on - strand, within GFF2491 at 2512.784 kb on + strand, within GFF2491 at 2512.784 kb on + strand, within GFF2491 at 2512.792 kb on - strand, within GFF2491 at 2512.803 kb on + strand, within GFF2491 at 2512.830 kb on + strand, within GFF2491 at 2512.887 kb on + strand, within GFF2491 at 2512.897 kb on - strand, within GFF2491 at 2512.899 kb on - strand, within GFF2491 at 2512.914 kb on + strand, within GFF2491 at 2512.918 kb on - strand, within GFF2491 at 2512.918 kb on - strand, within GFF2491 at 2512.922 kb on - strand, within GFF2491 at 2512.931 kb on + strand, within GFF2491 at 2512.943 kb on + strand, within GFF2491 at 2512.951 kb on - strand, within GFF2491 at 2512.952 kb on + strand, within GFF2491 at 2512.962 kb on + strand, within GFF2491 at 2512.975 kb on - strand, within GFF2491 at 2512.983 kb on + strand, within GFF2491 at 2513.009 kb on + strand, within GFF2491 at 2513.010 kb on - strand, within GFF2491 at 2513.010 kb on - strand, within GFF2491 at 2513.023 kb on + strand, within GFF2491 at 2513.033 kb on - strand, within GFF2491 at 2513.033 kb on - strand, within GFF2491 at 2513.052 kb on - strand, within GFF2491 at 2513.136 kb on - strand, within GFF2491 at 2513.141 kb on + strand, within GFF2491 at 2513.147 kb on - strand, within GFF2491 at 2513.155 kb on - strand, within GFF2491 at 2513.196 kb on + strand, within GFF2491 at 2513.216 kb on - strand at 2513.300 kb on - strand at 2513.308 kb on + strand at 2513.829 kb on + strand at 2513.837 kb on - strand at 2513.837 kb on - strand at 2513.881 kb on + strand at 2513.883 kb on - strand at 2513.887 kb on - strand at 2513.893 kb on + strand at 2513.893 kb on + strand at 2513.917 kb on + strand, within GFF2493 at 2513.917 kb on + strand, within GFF2493 at 2513.917 kb on + strand, within GFF2493 at 2513.917 kb on + strand, within GFF2493 at 2513.925 kb on - strand, within GFF2493 at 2513.925 kb on - strand, within GFF2493 at 2513.925 kb on - strand, within GFF2493 at 2513.925 kb on - strand, within GFF2493 at 2513.925 kb on - strand, within GFF2493 at 2513.925 kb on - strand, within GFF2493 at 2513.925 kb on - strand, within GFF2493 at 2513.964 kb on + strand, within GFF2493 at 2513.965 kb on + strand, within GFF2493 at 2513.965 kb on + strand, within GFF2493 at 2513.973 kb on - strand, within GFF2493 at 2513.973 kb on - strand, within GFF2493 at 2513.973 kb on - strand, within GFF2493 at 2513.973 kb on - strand, within GFF2493 at 2514.234 kb on - strand at 2514.243 kb on + strand at 2514.251 kb on - strand at 2514.251 kb on - strand at 2514.279 kb on + strand, within GFF2494 at 2514.285 kb on + strand, within GFF2494 at 2514.291 kb on + strand, within GFF2494
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with D-Glucose and Ammonium Sulfate remove 2,511,478 - +0.2 2,511,504 + +0.0 2,511,511 - -0.6 2,511,518 - +0.8 2,511,520 - -1.0 2,511,520 - -0.5 2,511,532 + +0.2 2,511,532 + +0.5 2,511,532 + -1.9 2,511,535 - +0.2 2,511,540 - -1.8 2,511,547 - +0.1 2,511,559 + -1.7 2,511,564 + +0.9 2,511,564 + +0.2 2,511,577 + -1.2 2,511,590 + -1.8 2,511,600 - -1.6 2,511,606 + -0.8 2,511,610 - GFF2489 0.11 -1.0 2,511,619 - GFF2489 0.14 -1.0 2,511,621 - GFF2489 0.15 +0.8 2,511,631 - GFF2489 0.19 +0.8 2,511,636 + GFF2489 0.21 -0.2 2,511,729 + GFF2489 0.59 -0.5 2,511,755 + GFF2489 0.70 +0.0 2,511,833 - +0.7 2,511,844 + +0.1 2,511,857 - -0.1 2,511,885 + -2.5 2,511,887 - +0.4 2,511,925 + -0.3 2,511,925 + +0.5 2,511,936 + -0.1 2,511,952 + -0.0 2,512,008 + GFF2490 0.19 -0.6 2,512,008 + GFF2490 0.19 -1.1 2,512,014 - GFF2490 0.20 -0.2 2,512,025 + GFF2490 0.23 -1.1 2,512,033 - GFF2490 0.24 -1.7 2,512,033 - GFF2490 0.24 -2.0 2,512,033 - GFF2490 0.24 +0.3 2,512,033 - GFF2490 0.24 -0.8 2,512,033 - GFF2490 0.24 -0.4 2,512,033 - GFF2490 0.24 -0.3 2,512,043 + GFF2490 0.26 -0.6 2,512,050 - GFF2490 0.28 -1.2 2,512,058 + GFF2490 0.29 -0.3 2,512,063 + GFF2490 0.30 -1.0 2,512,071 - GFF2490 0.32 +0.5 2,512,074 + GFF2490 0.32 +0.2 2,512,096 + GFF2490 0.37 -1.8 2,512,104 - GFF2490 0.38 -1.7 2,512,159 - GFF2490 0.49 +1.0 2,512,234 + GFF2490 0.64 -0.8 2,512,234 + GFF2490 0.64 +0.2 2,512,234 + GFF2490 0.64 -0.2 2,512,234 + GFF2490 0.64 -0.1 2,512,234 + GFF2490 0.64 +0.1 2,512,234 + GFF2490 0.64 -2.0 2,512,242 - GFF2490 0.66 -2.5 2,512,242 - GFF2490 0.66 -0.6 2,512,244 + GFF2490 0.66 +1.4 2,512,261 - GFF2490 0.69 -0.9 2,512,278 + GFF2490 0.73 -0.6 2,512,278 + GFF2490 0.73 -0.3 2,512,278 + GFF2490 0.73 +0.2 2,512,278 + GFF2490 0.73 -1.3 2,512,286 - GFF2490 0.74 -3.0 2,512,286 - GFF2490 0.74 +0.2 2,512,286 - GFF2490 0.74 -0.1 2,512,293 + GFF2490 0.76 -2.0 2,512,293 + GFF2490 0.76 +0.1 2,512,293 + GFF2490 0.76 +0.6 2,512,301 - GFF2490 0.77 +0.7 2,512,301 - GFF2490 0.77 +0.0 2,512,303 + GFF2490 0.78 -0.8 2,512,303 - GFF2490 0.78 -0.1 2,512,311 - GFF2490 0.79 -0.9 2,512,340 - GFF2490 0.85 -0.7 2,512,350 + GFF2490 0.87 -1.8 2,512,358 - GFF2490 0.89 +0.1 2,512,390 + +0.7 2,512,390 + +0.2 2,512,390 + -0.0 2,512,390 + -0.3 2,512,390 + -0.1 2,512,398 - -1.3 2,512,398 - +0.0 2,512,398 - -0.4 2,512,398 - -0.7 2,512,433 - -2.3 2,512,451 - -1.3 2,512,463 + -1.7 2,512,463 + -0.8 2,512,463 + +0.6 2,512,463 + -1.5 2,512,465 + -0.5 2,512,471 - -0.6 2,512,471 - +1.8 2,512,471 - -0.3 2,512,471 - -1.8 2,512,471 - -0.3 2,512,471 - -0.2 2,512,531 + -1.9 2,512,532 - -1.8 2,512,569 - GFF2491 0.11 -2.6 2,512,577 - GFF2491 0.12 -1.7 2,512,577 - GFF2491 0.12 -1.9 2,512,663 + GFF2491 0.23 -2.0 2,512,663 + GFF2491 0.23 -1.5 2,512,670 + GFF2491 0.24 -1.2 2,512,671 - GFF2491 0.24 -1.8 2,512,671 - GFF2491 0.24 -2.1 2,512,671 - GFF2491 0.24 -2.4 2,512,701 - GFF2491 0.27 -1.2 2,512,719 - GFF2491 0.30 -2.5 2,512,719 - GFF2491 0.30 -2.2 2,512,762 + GFF2491 0.35 -2.5 2,512,774 + GFF2491 0.36 +0.4 2,512,780 - GFF2491 0.37 -1.8 2,512,784 + GFF2491 0.38 -1.9 2,512,784 + GFF2491 0.38 -2.4 2,512,792 - GFF2491 0.39 -1.6 2,512,803 + GFF2491 0.40 -1.9 2,512,830 + GFF2491 0.43 -2.2 2,512,887 + GFF2491 0.50 -2.7 2,512,897 - GFF2491 0.51 -2.3 2,512,899 - GFF2491 0.52 -2.1 2,512,914 + GFF2491 0.54 -1.6 2,512,918 - GFF2491 0.54 -1.9 2,512,918 - GFF2491 0.54 -1.1 2,512,922 - GFF2491 0.55 -1.2 2,512,931 + GFF2491 0.56 -1.3 2,512,943 + GFF2491 0.57 +0.2 2,512,951 - GFF2491 0.58 -0.7 2,512,952 + GFF2491 0.58 -1.5 2,512,962 + GFF2491 0.59 -1.3 2,512,975 - GFF2491 0.61 -2.1 2,512,983 + GFF2491 0.62 -1.8 2,513,009 + GFF2491 0.65 -2.6 2,513,010 - GFF2491 0.65 -1.2 2,513,010 - GFF2491 0.65 -0.3 2,513,023 + GFF2491 0.67 -3.0 2,513,033 - GFF2491 0.68 -2.4 2,513,033 - GFF2491 0.68 -0.4 2,513,052 - GFF2491 0.70 -0.7 2,513,136 - GFF2491 0.81 -0.1 2,513,141 + GFF2491 0.81 -1.8 2,513,147 - GFF2491 0.82 -1.6 2,513,155 - GFF2491 0.83 -0.5 2,513,196 + GFF2491 0.88 -1.6 2,513,216 - -2.9 2,513,300 - -0.8 2,513,308 + +0.4 2,513,829 + -1.3 2,513,837 - -0.3 2,513,837 - +2.0 2,513,881 + +1.5 2,513,883 - -0.5 2,513,887 - +0.9 2,513,893 + -1.5 2,513,893 + -0.8 2,513,917 + GFF2493 0.17 -1.9 2,513,917 + GFF2493 0.17 -2.4 2,513,917 + GFF2493 0.17 -1.1 2,513,917 + GFF2493 0.17 +0.3 2,513,925 - GFF2493 0.19 -0.6 2,513,925 - GFF2493 0.19 -0.1 2,513,925 - GFF2493 0.19 +1.2 2,513,925 - GFF2493 0.19 +1.2 2,513,925 - GFF2493 0.19 -1.2 2,513,925 - GFF2493 0.19 +0.2 2,513,925 - GFF2493 0.19 -1.7 2,513,964 + GFF2493 0.32 -0.6 2,513,965 + GFF2493 0.32 +0.9 2,513,965 + GFF2493 0.32 -0.0 2,513,973 - GFF2493 0.35 -0.7 2,513,973 - GFF2493 0.35 -0.9 2,513,973 - GFF2493 0.35 -2.9 2,513,973 - GFF2493 0.35 +1.9 2,514,234 - -0.0 2,514,243 + +0.1 2,514,251 - -0.8 2,514,251 - +1.1 2,514,279 + GFF2494 0.11 -1.3 2,514,285 + GFF2494 0.12 -0.5 2,514,291 + GFF2494 0.13 +0.5
Or see this region's nucleotide sequence