Experiment: EMM_noCarbon with D-Glucose and Ammonium Sulfate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2370 and GFF2371 are separated by 244 nucleotides GFF2371 and GFF2372 are separated by 19 nucleotides GFF2372 and GFF2373 are separated by 3 nucleotides GFF2373 and GFF2374 are separated by 56 nucleotides
GFF2370 - Anthranilate synthase, aminase component (EC 4.1.3.27), at 2,393,414 to 2,394,937
GFF2370
GFF2371 - hypothetical protein, at 2,395,182 to 2,395,418
GFF2371
GFF2372 - RNA binding S1 domain protein, at 2,395,438 to 2,396,166
GFF2372
GFF2373 - hypothetical protein, at 2,396,170 to 2,396,940
GFF2373
GFF2374 - P-hydroxybenzoate hydroxylase (EC 1.14.13.2), at 2,396,997 to 2,398,178
GFF2374
Position (kb)
2395
2396
2397 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2394.480 kb on - strand, within GFF2370 at 2394.504 kb on + strand, within GFF2370 at 2394.543 kb on + strand, within GFF2370 at 2394.548 kb on + strand, within GFF2370 at 2394.548 kb on + strand, within GFF2370 at 2394.613 kb on + strand, within GFF2370 at 2394.621 kb on - strand, within GFF2370 at 2394.646 kb on - strand, within GFF2370 at 2394.658 kb on - strand, within GFF2370 at 2394.658 kb on - strand, within GFF2370 at 2394.751 kb on + strand, within GFF2370 at 2394.756 kb on + strand, within GFF2370 at 2394.764 kb on - strand, within GFF2370 at 2394.769 kb on - strand, within GFF2370 at 2394.774 kb on - strand, within GFF2370 at 2394.779 kb on + strand, within GFF2370 at 2394.779 kb on - strand, within GFF2370 at 2394.799 kb on + strand at 2394.871 kb on - strand at 2394.880 kb on + strand at 2394.899 kb on + strand at 2394.899 kb on + strand at 2394.899 kb on + strand at 2394.965 kb on + strand at 2395.010 kb on - strand at 2395.024 kb on + strand at 2395.058 kb on - strand at 2395.129 kb on + strand at 2395.129 kb on + strand at 2395.242 kb on - strand, within GFF2371 at 2395.320 kb on - strand, within GFF2371 at 2395.323 kb on + strand, within GFF2371 at 2395.331 kb on - strand, within GFF2371 at 2395.338 kb on + strand, within GFF2371 at 2395.338 kb on - strand, within GFF2371 at 2395.346 kb on - strand, within GFF2371 at 2395.377 kb on + strand, within GFF2371 at 2395.385 kb on - strand, within GFF2371 at 2395.395 kb on - strand at 2395.405 kb on - strand at 2395.406 kb on + strand at 2395.420 kb on - strand at 2395.429 kb on + strand at 2395.437 kb on - strand at 2395.516 kb on - strand, within GFF2372 at 2395.528 kb on - strand, within GFF2372 at 2395.540 kb on - strand, within GFF2372 at 2395.583 kb on + strand, within GFF2372 at 2395.588 kb on + strand, within GFF2372 at 2395.588 kb on + strand, within GFF2372 at 2395.590 kb on - strand, within GFF2372 at 2395.591 kb on - strand, within GFF2372 at 2395.593 kb on + strand, within GFF2372 at 2395.593 kb on + strand, within GFF2372 at 2395.601 kb on - strand, within GFF2372 at 2395.603 kb on + strand, within GFF2372 at 2395.618 kb on + strand, within GFF2372 at 2395.628 kb on + strand, within GFF2372 at 2395.641 kb on - strand, within GFF2372 at 2395.702 kb on + strand, within GFF2372 at 2395.737 kb on + strand, within GFF2372 at 2395.750 kb on + strand, within GFF2372 at 2395.780 kb on + strand, within GFF2372 at 2395.789 kb on - strand, within GFF2372 at 2395.793 kb on - strand, within GFF2372 at 2395.794 kb on + strand, within GFF2372 at 2395.802 kb on - strand, within GFF2372 at 2395.824 kb on - strand, within GFF2372 at 2395.826 kb on + strand, within GFF2372 at 2395.829 kb on - strand, within GFF2372 at 2395.836 kb on + strand, within GFF2372 at 2395.839 kb on - strand, within GFF2372 at 2395.839 kb on - strand, within GFF2372 at 2395.844 kb on - strand, within GFF2372 at 2395.878 kb on + strand, within GFF2372 at 2395.882 kb on + strand, within GFF2372 at 2395.893 kb on + strand, within GFF2372 at 2395.901 kb on - strand, within GFF2372 at 2395.906 kb on + strand, within GFF2372 at 2395.914 kb on + strand, within GFF2372 at 2395.914 kb on + strand, within GFF2372 at 2395.917 kb on - strand, within GFF2372 at 2395.922 kb on - strand, within GFF2372 at 2395.940 kb on + strand, within GFF2372 at 2395.949 kb on + strand, within GFF2372 at 2395.957 kb on - strand, within GFF2372 at 2395.966 kb on - strand, within GFF2372 at 2395.968 kb on + strand, within GFF2372 at 2395.976 kb on - strand, within GFF2372 at 2395.993 kb on + strand, within GFF2372 at 2396.015 kb on - strand, within GFF2372 at 2396.033 kb on - strand, within GFF2372 at 2396.043 kb on + strand, within GFF2372 at 2396.043 kb on + strand, within GFF2372 at 2396.043 kb on + strand, within GFF2372 at 2396.043 kb on + strand, within GFF2372 at 2396.043 kb on + strand, within GFF2372 at 2396.049 kb on + strand, within GFF2372 at 2396.049 kb on + strand, within GFF2372 at 2396.051 kb on - strand, within GFF2372 at 2396.051 kb on - strand, within GFF2372 at 2396.051 kb on - strand, within GFF2372 at 2396.051 kb on - strand, within GFF2372 at 2396.051 kb on - strand, within GFF2372 at 2396.051 kb on - strand, within GFF2372 at 2396.051 kb on - strand, within GFF2372 at 2396.051 kb on - strand, within GFF2372 at 2396.054 kb on + strand, within GFF2372 at 2396.054 kb on + strand, within GFF2372 at 2396.054 kb on + strand, within GFF2372 at 2396.056 kb on - strand, within GFF2372 at 2396.057 kb on - strand, within GFF2372 at 2396.057 kb on - strand, within GFF2372 at 2396.057 kb on - strand, within GFF2372 at 2396.059 kb on + strand, within GFF2372 at 2396.062 kb on - strand, within GFF2372 at 2396.062 kb on - strand, within GFF2372 at 2396.062 kb on - strand, within GFF2372 at 2396.062 kb on - strand, within GFF2372 at 2396.062 kb on - strand, within GFF2372 at 2396.094 kb on + strand at 2396.094 kb on + strand at 2396.096 kb on - strand at 2396.099 kb on + strand at 2396.099 kb on + strand at 2396.099 kb on + strand at 2396.099 kb on + strand at 2396.107 kb on - strand at 2396.107 kb on - strand at 2396.109 kb on + strand at 2396.109 kb on + strand at 2396.109 kb on + strand at 2396.109 kb on + strand at 2396.109 kb on + strand at 2396.109 kb on + strand at 2396.109 kb on + strand at 2396.117 kb on - strand at 2396.117 kb on - strand at 2396.117 kb on - strand at 2396.121 kb on - strand at 2396.133 kb on + strand at 2396.136 kb on + strand at 2396.144 kb on - strand at 2396.151 kb on + strand at 2396.151 kb on + strand at 2396.154 kb on - strand at 2396.159 kb on - strand at 2396.159 kb on - strand at 2396.159 kb on - strand at 2396.167 kb on + strand at 2396.172 kb on + strand at 2396.177 kb on + strand at 2396.204 kb on - strand at 2396.215 kb on + strand at 2396.223 kb on - strand at 2396.223 kb on - strand at 2396.259 kb on + strand, within GFF2373 at 2396.259 kb on + strand, within GFF2373 at 2396.259 kb on + strand, within GFF2373 at 2396.264 kb on + strand, within GFF2373 at 2396.267 kb on - strand, within GFF2373 at 2396.267 kb on - strand, within GFF2373 at 2396.267 kb on - strand, within GFF2373 at 2396.288 kb on + strand, within GFF2373 at 2396.302 kb on - strand, within GFF2373 at 2396.316 kb on + strand, within GFF2373 at 2396.332 kb on - strand, within GFF2373 at 2396.373 kb on - strand, within GFF2373 at 2396.409 kb on + strand, within GFF2373 at 2396.420 kb on + strand, within GFF2373 at 2396.467 kb on + strand, within GFF2373 at 2396.494 kb on - strand, within GFF2373 at 2396.566 kb on - strand, within GFF2373 at 2396.579 kb on + strand, within GFF2373 at 2396.615 kb on + strand, within GFF2373 at 2396.618 kb on - strand, within GFF2373 at 2396.658 kb on + strand, within GFF2373 at 2396.668 kb on + strand, within GFF2373 at 2396.671 kb on - strand, within GFF2373 at 2396.700 kb on - strand, within GFF2373 at 2396.722 kb on - strand, within GFF2373 at 2396.738 kb on + strand, within GFF2373 at 2396.746 kb on - strand, within GFF2373 at 2396.817 kb on - strand, within GFF2373 at 2396.817 kb on - strand, within GFF2373 at 2396.838 kb on - strand, within GFF2373 at 2396.860 kb on - strand, within GFF2373 at 2396.944 kb on - strand at 2396.954 kb on - strand at 2396.955 kb on - strand at 2396.976 kb on + strand at 2396.981 kb on + strand at 2396.986 kb on + strand at 2397.037 kb on + strand at 2397.076 kb on + strand at 2397.094 kb on - strand at 2397.098 kb on + strand at 2397.166 kb on + strand, within GFF2374
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with D-Glucose and Ammonium Sulfate remove 2,394,480 - GFF2370 0.70 -2.7 2,394,504 + GFF2370 0.72 -2.1 2,394,543 + GFF2370 0.74 -1.9 2,394,548 + GFF2370 0.74 -3.3 2,394,548 + GFF2370 0.74 -3.0 2,394,613 + GFF2370 0.79 -1.5 2,394,621 - GFF2370 0.79 -0.7 2,394,646 - GFF2370 0.81 -1.9 2,394,658 - GFF2370 0.82 -1.8 2,394,658 - GFF2370 0.82 -1.4 2,394,751 + GFF2370 0.88 -1.4 2,394,756 + GFF2370 0.88 -2.8 2,394,764 - GFF2370 0.89 -3.1 2,394,769 - GFF2370 0.89 -0.6 2,394,774 - GFF2370 0.89 -0.6 2,394,779 + GFF2370 0.90 -2.3 2,394,779 - GFF2370 0.90 -0.5 2,394,799 + -2.4 2,394,871 - -3.0 2,394,880 + +0.1 2,394,899 + -2.9 2,394,899 + -0.1 2,394,899 + -2.6 2,394,965 + +0.4 2,395,010 - -0.4 2,395,024 + -0.1 2,395,058 - -0.9 2,395,129 + +0.3 2,395,129 + +0.5 2,395,242 - GFF2371 0.25 -1.5 2,395,320 - GFF2371 0.58 +0.6 2,395,323 + GFF2371 0.59 -1.7 2,395,331 - GFF2371 0.63 -0.7 2,395,338 + GFF2371 0.66 -0.2 2,395,338 - GFF2371 0.66 -1.4 2,395,346 - GFF2371 0.69 +0.9 2,395,377 + GFF2371 0.82 +0.6 2,395,385 - GFF2371 0.86 +0.4 2,395,395 - -1.7 2,395,405 - +1.1 2,395,406 + -0.0 2,395,420 - +0.2 2,395,429 + -1.7 2,395,437 - +0.8 2,395,516 - GFF2372 0.11 +0.6 2,395,528 - GFF2372 0.12 -0.8 2,395,540 - GFF2372 0.14 -0.2 2,395,583 + GFF2372 0.20 -0.1 2,395,588 + GFF2372 0.21 +0.4 2,395,588 + GFF2372 0.21 +1.7 2,395,590 - GFF2372 0.21 -0.5 2,395,591 - GFF2372 0.21 -0.2 2,395,593 + GFF2372 0.21 +0.6 2,395,593 + GFF2372 0.21 +0.1 2,395,601 - GFF2372 0.22 -1.4 2,395,603 + GFF2372 0.23 +0.4 2,395,618 + GFF2372 0.25 +1.7 2,395,628 + GFF2372 0.26 +0.8 2,395,641 - GFF2372 0.28 +0.5 2,395,702 + GFF2372 0.36 -0.9 2,395,737 + GFF2372 0.41 +0.1 2,395,750 + GFF2372 0.43 +0.6 2,395,780 + GFF2372 0.47 -0.1 2,395,789 - GFF2372 0.48 +0.7 2,395,793 - GFF2372 0.49 -0.9 2,395,794 + GFF2372 0.49 +0.1 2,395,802 - GFF2372 0.50 +0.4 2,395,824 - GFF2372 0.53 +0.1 2,395,826 + GFF2372 0.53 +0.4 2,395,829 - GFF2372 0.54 +0.5 2,395,836 + GFF2372 0.55 +1.0 2,395,839 - GFF2372 0.55 +1.6 2,395,839 - GFF2372 0.55 +0.8 2,395,844 - GFF2372 0.56 +1.1 2,395,878 + GFF2372 0.60 +0.3 2,395,882 + GFF2372 0.61 +0.0 2,395,893 + GFF2372 0.62 -0.6 2,395,901 - GFF2372 0.64 -0.9 2,395,906 + GFF2372 0.64 -1.0 2,395,914 + GFF2372 0.65 -0.7 2,395,914 + GFF2372 0.65 +1.4 2,395,917 - GFF2372 0.66 -1.2 2,395,922 - GFF2372 0.66 +2.0 2,395,940 + GFF2372 0.69 +1.0 2,395,949 + GFF2372 0.70 -0.4 2,395,957 - GFF2372 0.71 -0.2 2,395,966 - GFF2372 0.72 -1.3 2,395,968 + GFF2372 0.73 +0.6 2,395,976 - GFF2372 0.74 +1.4 2,395,993 + GFF2372 0.76 -0.2 2,396,015 - GFF2372 0.79 +0.8 2,396,033 - GFF2372 0.82 -1.8 2,396,043 + GFF2372 0.83 +1.6 2,396,043 + GFF2372 0.83 +0.7 2,396,043 + GFF2372 0.83 +0.6 2,396,043 + GFF2372 0.83 -0.7 2,396,043 + GFF2372 0.83 +0.6 2,396,049 + GFF2372 0.84 -1.5 2,396,049 + GFF2372 0.84 +1.6 2,396,051 - GFF2372 0.84 +1.0 2,396,051 - GFF2372 0.84 -0.2 2,396,051 - GFF2372 0.84 +0.5 2,396,051 - GFF2372 0.84 -0.3 2,396,051 - GFF2372 0.84 +0.4 2,396,051 - GFF2372 0.84 -2.4 2,396,051 - GFF2372 0.84 +0.3 2,396,051 - GFF2372 0.84 -0.2 2,396,054 + GFF2372 0.84 +1.9 2,396,054 + GFF2372 0.84 +0.6 2,396,054 + GFF2372 0.84 -1.6 2,396,056 - GFF2372 0.85 -0.2 2,396,057 - GFF2372 0.85 -1.1 2,396,057 - GFF2372 0.85 -0.3 2,396,057 - GFF2372 0.85 +0.8 2,396,059 + GFF2372 0.85 +0.4 2,396,062 - GFF2372 0.86 -0.8 2,396,062 - GFF2372 0.86 -0.0 2,396,062 - GFF2372 0.86 +0.1 2,396,062 - GFF2372 0.86 +0.2 2,396,062 - GFF2372 0.86 +0.6 2,396,094 + -1.2 2,396,094 + -1.6 2,396,096 - -0.6 2,396,099 + +0.1 2,396,099 + +0.9 2,396,099 + +0.8 2,396,099 + -0.6 2,396,107 - +0.9 2,396,107 - -0.6 2,396,109 + +0.8 2,396,109 + -1.6 2,396,109 + +1.1 2,396,109 + -0.5 2,396,109 + +0.7 2,396,109 + -1.0 2,396,109 + +0.3 2,396,117 - -0.9 2,396,117 - +0.4 2,396,117 - +0.5 2,396,121 - +0.7 2,396,133 + -1.6 2,396,136 + +0.5 2,396,144 - -1.2 2,396,151 + +0.3 2,396,151 + -2.2 2,396,154 - +0.1 2,396,159 - +1.1 2,396,159 - +0.1 2,396,159 - -0.2 2,396,167 + -0.4 2,396,172 + +0.3 2,396,177 + -0.8 2,396,204 - -0.8 2,396,215 + -0.7 2,396,223 - -0.7 2,396,223 - +0.7 2,396,259 + GFF2373 0.12 +0.3 2,396,259 + GFF2373 0.12 -0.2 2,396,259 + GFF2373 0.12 +0.6 2,396,264 + GFF2373 0.12 -1.2 2,396,267 - GFF2373 0.13 +0.9 2,396,267 - GFF2373 0.13 -0.5 2,396,267 - GFF2373 0.13 -0.3 2,396,288 + GFF2373 0.15 +1.5 2,396,302 - GFF2373 0.17 -1.1 2,396,316 + GFF2373 0.19 +0.4 2,396,332 - GFF2373 0.21 +1.1 2,396,373 - GFF2373 0.26 +1.0 2,396,409 + GFF2373 0.31 -0.3 2,396,420 + GFF2373 0.32 -0.7 2,396,467 + GFF2373 0.39 +1.4 2,396,494 - GFF2373 0.42 -0.1 2,396,566 - GFF2373 0.51 +0.2 2,396,579 + GFF2373 0.53 -0.7 2,396,615 + GFF2373 0.58 -0.4 2,396,618 - GFF2373 0.58 +1.4 2,396,658 + GFF2373 0.63 -2.0 2,396,668 + GFF2373 0.65 -0.4 2,396,671 - GFF2373 0.65 +0.3 2,396,700 - GFF2373 0.69 -0.8 2,396,722 - GFF2373 0.72 -0.7 2,396,738 + GFF2373 0.74 +0.3 2,396,746 - GFF2373 0.75 +0.2 2,396,817 - GFF2373 0.84 +0.7 2,396,817 - GFF2373 0.84 -0.8 2,396,838 - GFF2373 0.87 +0.3 2,396,860 - GFF2373 0.89 -1.0 2,396,944 - -0.4 2,396,954 - -0.7 2,396,955 - +0.9 2,396,976 + +0.5 2,396,981 + +0.3 2,396,986 + -2.2 2,397,037 + +0.4 2,397,076 + -0.1 2,397,094 - -0.1 2,397,098 + +0.2 2,397,166 + GFF2374 0.14 +0.7
Or see this region's nucleotide sequence