Experiment: EMM_noCarbon with D-Glucose and Ammonium Sulfate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2060 and GFF2061 are separated by 118 nucleotides GFF2061 and GFF2062 are separated by 194 nucleotides
GFF2060 - 2-isopropylmalate synthase (EC 2.3.3.13), at 2,096,811 to 2,098,484
GFF2060
GFF2061 - Putative peptidase, at 2,098,603 to 2,099,985
GFF2061
GFF2062 - Proton/glutamate symporter @ Sodium/glutamate symporter, at 2,100,180 to 2,101,481
GFF2062
Position (kb)
2098
2099
2100 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2097.619 kb on - strand, within GFF2060 at 2097.694 kb on - strand, within GFF2060 at 2097.706 kb on + strand, within GFF2060 at 2097.708 kb on - strand, within GFF2060 at 2097.719 kb on - strand, within GFF2060 at 2097.734 kb on + strand, within GFF2060 at 2097.742 kb on + strand, within GFF2060 at 2097.742 kb on - strand, within GFF2060 at 2097.743 kb on - strand, within GFF2060 at 2097.782 kb on + strand, within GFF2060 at 2097.785 kb on - strand, within GFF2060 at 2097.878 kb on + strand, within GFF2060 at 2097.878 kb on + strand, within GFF2060 at 2097.878 kb on + strand, within GFF2060 at 2097.878 kb on + strand, within GFF2060 at 2097.878 kb on + strand, within GFF2060 at 2097.878 kb on + strand, within GFF2060 at 2097.886 kb on - strand, within GFF2060 at 2097.886 kb on - strand, within GFF2060 at 2097.886 kb on - strand, within GFF2060 at 2097.886 kb on - strand, within GFF2060 at 2097.886 kb on - strand, within GFF2060 at 2097.886 kb on - strand, within GFF2060 at 2097.897 kb on - strand, within GFF2060 at 2097.915 kb on + strand, within GFF2060 at 2097.915 kb on + strand, within GFF2060 at 2097.915 kb on + strand, within GFF2060 at 2097.915 kb on + strand, within GFF2060 at 2097.915 kb on + strand, within GFF2060 at 2097.915 kb on + strand, within GFF2060 at 2097.915 kb on + strand, within GFF2060 at 2097.915 kb on + strand, within GFF2060 at 2097.915 kb on - strand, within GFF2060 at 2097.923 kb on - strand, within GFF2060 at 2097.923 kb on - strand, within GFF2060 at 2097.936 kb on + strand, within GFF2060 at 2097.963 kb on + strand, within GFF2060 at 2097.963 kb on + strand, within GFF2060 at 2097.995 kb on + strand, within GFF2060 at 2097.995 kb on + strand, within GFF2060 at 2098.003 kb on + strand, within GFF2060 at 2098.003 kb on - strand, within GFF2060 at 2098.003 kb on - strand, within GFF2060 at 2098.003 kb on - strand, within GFF2060 at 2098.010 kb on - strand, within GFF2060 at 2098.013 kb on + strand, within GFF2060 at 2098.021 kb on - strand, within GFF2060 at 2098.044 kb on - strand, within GFF2060 at 2098.052 kb on + strand, within GFF2060 at 2098.105 kb on + strand, within GFF2060 at 2098.116 kb on + strand, within GFF2060 at 2098.141 kb on + strand, within GFF2060 at 2098.147 kb on - strand, within GFF2060 at 2098.149 kb on - strand, within GFF2060 at 2098.149 kb on - strand, within GFF2060 at 2098.223 kb on - strand, within GFF2060 at 2098.236 kb on + strand, within GFF2060 at 2098.249 kb on + strand, within GFF2060 at 2098.251 kb on - strand, within GFF2060 at 2098.253 kb on + strand, within GFF2060 at 2098.289 kb on + strand, within GFF2060 at 2098.304 kb on - strand, within GFF2060 at 2098.304 kb on - strand, within GFF2060 at 2098.393 kb on - strand at 2098.397 kb on + strand at 2098.436 kb on + strand at 2098.436 kb on + strand at 2098.436 kb on + strand at 2098.436 kb on + strand at 2098.436 kb on + strand at 2098.436 kb on + strand at 2098.436 kb on + strand at 2098.436 kb on + strand at 2098.436 kb on + strand at 2098.436 kb on + strand at 2098.436 kb on + strand at 2098.436 kb on + strand at 2098.436 kb on + strand at 2098.436 kb on + strand at 2098.444 kb on - strand at 2098.444 kb on - strand at 2098.444 kb on - strand at 2098.444 kb on - strand at 2098.444 kb on - strand at 2098.444 kb on - strand at 2098.444 kb on - strand at 2098.444 kb on - strand at 2098.463 kb on + strand at 2098.501 kb on - strand at 2098.524 kb on - strand at 2098.549 kb on - strand at 2098.551 kb on + strand at 2098.556 kb on + strand at 2098.556 kb on + strand at 2098.570 kb on - strand at 2098.579 kb on + strand at 2098.604 kb on + strand at 2098.628 kb on + strand at 2098.628 kb on - strand at 2098.638 kb on + strand at 2098.638 kb on - strand at 2098.646 kb on - strand at 2098.697 kb on - strand at 2098.727 kb on - strand at 2098.736 kb on - strand at 2098.758 kb on + strand, within GFF2061 at 2098.891 kb on + strand, within GFF2061 at 2098.904 kb on - strand, within GFF2061 at 2098.928 kb on + strand, within GFF2061 at 2098.931 kb on - strand, within GFF2061 at 2098.936 kb on - strand, within GFF2061 at 2098.988 kb on + strand, within GFF2061 at 2098.996 kb on - strand, within GFF2061 at 2099.015 kb on + strand, within GFF2061 at 2099.022 kb on + strand, within GFF2061 at 2099.022 kb on + strand, within GFF2061 at 2099.060 kb on + strand, within GFF2061 at 2099.068 kb on - strand, within GFF2061 at 2099.108 kb on - strand, within GFF2061 at 2099.118 kb on - strand, within GFF2061 at 2099.129 kb on + strand, within GFF2061 at 2099.129 kb on + strand, within GFF2061 at 2099.138 kb on + strand, within GFF2061 at 2099.192 kb on + strand, within GFF2061 at 2099.192 kb on + strand, within GFF2061 at 2099.192 kb on + strand, within GFF2061 at 2099.200 kb on - strand, within GFF2061 at 2099.200 kb on - strand, within GFF2061 at 2099.224 kb on + strand, within GFF2061 at 2099.239 kb on - strand, within GFF2061 at 2099.257 kb on - strand, within GFF2061 at 2099.259 kb on + strand, within GFF2061 at 2099.311 kb on + strand, within GFF2061 at 2099.372 kb on + strand, within GFF2061 at 2099.419 kb on - strand, within GFF2061 at 2099.433 kb on + strand, within GFF2061 at 2099.440 kb on - strand, within GFF2061 at 2099.440 kb on - strand, within GFF2061 at 2099.440 kb on - strand, within GFF2061 at 2099.441 kb on - strand, within GFF2061 at 2099.462 kb on - strand, within GFF2061 at 2099.462 kb on - strand, within GFF2061 at 2099.476 kb on - strand, within GFF2061 at 2099.494 kb on + strand, within GFF2061 at 2099.519 kb on + strand, within GFF2061 at 2099.525 kb on - strand, within GFF2061 at 2099.527 kb on - strand, within GFF2061 at 2099.527 kb on - strand, within GFF2061 at 2099.527 kb on - strand, within GFF2061 at 2099.539 kb on - strand, within GFF2061 at 2099.583 kb on + strand, within GFF2061 at 2099.583 kb on + strand, within GFF2061 at 2099.697 kb on + strand, within GFF2061 at 2099.697 kb on + strand, within GFF2061 at 2099.822 kb on + strand, within GFF2061 at 2099.824 kb on + strand, within GFF2061 at 2099.835 kb on + strand, within GFF2061 at 2099.851 kb on - strand at 2099.862 kb on + strand at 2099.924 kb on + strand at 2099.932 kb on - strand at 2099.950 kb on - strand at 2099.950 kb on - strand at 2099.995 kb on - strand at 2100.084 kb on - strand at 2100.089 kb on - strand at 2100.090 kb on + strand at 2100.090 kb on + strand at 2100.090 kb on + strand at 2100.098 kb on - strand at 2100.099 kb on - strand at 2100.141 kb on + strand at 2100.151 kb on - strand at 2100.199 kb on + strand at 2100.228 kb on + strand at 2100.281 kb on - strand at 2100.307 kb on + strand at 2100.308 kb on + strand at 2100.308 kb on + strand at 2100.365 kb on + strand, within GFF2062 at 2100.373 kb on - strand, within GFF2062 at 2100.410 kb on + strand, within GFF2062 at 2100.410 kb on + strand, within GFF2062 at 2100.418 kb on - strand, within GFF2062 at 2100.440 kb on - strand, within GFF2062 at 2100.443 kb on + strand, within GFF2062 at 2100.443 kb on + strand, within GFF2062 at 2100.486 kb on - strand, within GFF2062 at 2100.486 kb on - strand, within GFF2062 at 2100.486 kb on - strand, within GFF2062 at 2100.519 kb on + strand, within GFF2062 at 2100.519 kb on + strand, within GFF2062 at 2100.519 kb on + strand, within GFF2062 at 2100.519 kb on + strand, within GFF2062 at 2100.527 kb on - strand, within GFF2062 at 2100.527 kb on - strand, within GFF2062 at 2100.529 kb on + strand, within GFF2062 at 2100.532 kb on - strand, within GFF2062 at 2100.588 kb on + strand, within GFF2062 at 2100.588 kb on + strand, within GFF2062 at 2100.614 kb on - strand, within GFF2062 at 2100.709 kb on + strand, within GFF2062 at 2100.734 kb on + strand, within GFF2062 at 2100.743 kb on - strand, within GFF2062 at 2100.786 kb on + strand, within GFF2062 at 2100.786 kb on + strand, within GFF2062 at 2100.786 kb on + strand, within GFF2062 at 2100.786 kb on + strand, within GFF2062 at 2100.786 kb on + strand, within GFF2062 at 2100.786 kb on + strand, within GFF2062 at 2100.794 kb on - strand, within GFF2062 at 2100.794 kb on - strand, within GFF2062 at 2100.794 kb on - strand, within GFF2062 at 2100.794 kb on - strand, within GFF2062 at 2100.794 kb on - strand, within GFF2062 at 2100.794 kb on - strand, within GFF2062 at 2100.794 kb on - strand, within GFF2062 at 2100.794 kb on - strand, within GFF2062 at 2100.794 kb on - strand, within GFF2062 at 2100.794 kb on - strand, within GFF2062 at 2100.794 kb on - strand, within GFF2062 at 2100.794 kb on - strand, within GFF2062 at 2100.794 kb on - strand, within GFF2062 at 2100.794 kb on - strand, within GFF2062 at 2100.796 kb on + strand, within GFF2062 at 2100.799 kb on - strand, within GFF2062 at 2100.799 kb on - strand, within GFF2062 at 2100.805 kb on - strand, within GFF2062 at 2100.853 kb on + strand, within GFF2062 at 2100.861 kb on - strand, within GFF2062 at 2100.878 kb on + strand, within GFF2062 at 2100.878 kb on + strand, within GFF2062 at 2100.886 kb on - strand, within GFF2062 at 2100.894 kb on - strand, within GFF2062 at 2100.904 kb on + strand, within GFF2062 at 2100.909 kb on + strand, within GFF2062 at 2100.955 kb on + strand, within GFF2062 at 2100.970 kb on - strand, within GFF2062
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with D-Glucose and Ammonium Sulfate remove 2,097,619 - GFF2060 0.48 -2.3 2,097,694 - GFF2060 0.53 -2.0 2,097,706 + GFF2060 0.53 -1.8 2,097,708 - GFF2060 0.54 -0.6 2,097,719 - GFF2060 0.54 -1.7 2,097,734 + GFF2060 0.55 -1.6 2,097,742 + GFF2060 0.56 -3.3 2,097,742 - GFF2060 0.56 -1.4 2,097,743 - GFF2060 0.56 -2.1 2,097,782 + GFF2060 0.58 -1.8 2,097,785 - GFF2060 0.58 -4.2 2,097,878 + GFF2060 0.64 -2.2 2,097,878 + GFF2060 0.64 -2.7 2,097,878 + GFF2060 0.64 -2.0 2,097,878 + GFF2060 0.64 -2.8 2,097,878 + GFF2060 0.64 -2.4 2,097,878 + GFF2060 0.64 -2.2 2,097,886 - GFF2060 0.64 -2.7 2,097,886 - GFF2060 0.64 -0.6 2,097,886 - GFF2060 0.64 -0.6 2,097,886 - GFF2060 0.64 -2.6 2,097,886 - GFF2060 0.64 -2.0 2,097,886 - GFF2060 0.64 -3.2 2,097,897 - GFF2060 0.65 -0.8 2,097,915 + GFF2060 0.66 -1.4 2,097,915 + GFF2060 0.66 -1.8 2,097,915 + GFF2060 0.66 -2.5 2,097,915 + GFF2060 0.66 -2.7 2,097,915 + GFF2060 0.66 -3.3 2,097,915 + GFF2060 0.66 -1.2 2,097,915 + GFF2060 0.66 -3.1 2,097,915 + GFF2060 0.66 -3.0 2,097,915 - GFF2060 0.66 -1.4 2,097,923 - GFF2060 0.66 +0.0 2,097,923 - GFF2060 0.66 -2.8 2,097,936 + GFF2060 0.67 -2.0 2,097,963 + GFF2060 0.69 -1.1 2,097,963 + GFF2060 0.69 +0.2 2,097,995 + GFF2060 0.71 -1.6 2,097,995 + GFF2060 0.71 -1.1 2,098,003 + GFF2060 0.71 -1.0 2,098,003 - GFF2060 0.71 -0.4 2,098,003 - GFF2060 0.71 -0.2 2,098,003 - GFF2060 0.71 -2.2 2,098,010 - GFF2060 0.72 -2.0 2,098,013 + GFF2060 0.72 -3.7 2,098,021 - GFF2060 0.72 -2.3 2,098,044 - GFF2060 0.74 -2.6 2,098,052 + GFF2060 0.74 -2.7 2,098,105 + GFF2060 0.77 -2.1 2,098,116 + GFF2060 0.78 -3.4 2,098,141 + GFF2060 0.79 -2.8 2,098,147 - GFF2060 0.80 -1.5 2,098,149 - GFF2060 0.80 -0.6 2,098,149 - GFF2060 0.80 -2.3 2,098,223 - GFF2060 0.84 -2.7 2,098,236 + GFF2060 0.85 -3.2 2,098,249 + GFF2060 0.86 -1.1 2,098,251 - GFF2060 0.86 -0.9 2,098,253 + GFF2060 0.86 -2.1 2,098,289 + GFF2060 0.88 -3.7 2,098,304 - GFF2060 0.89 -3.0 2,098,304 - GFF2060 0.89 -2.4 2,098,393 - -4.0 2,098,397 + -1.4 2,098,436 + -0.2 2,098,436 + -2.3 2,098,436 + -1.8 2,098,436 + -3.7 2,098,436 + -2.6 2,098,436 + -1.6 2,098,436 + -2.4 2,098,436 + -1.5 2,098,436 + -2.4 2,098,436 + -3.0 2,098,436 + -1.7 2,098,436 + -2.0 2,098,436 + -1.8 2,098,436 + -2.7 2,098,444 - -2.1 2,098,444 - -2.5 2,098,444 - -2.0 2,098,444 - -1.3 2,098,444 - -2.8 2,098,444 - -2.2 2,098,444 - -4.2 2,098,444 - -2.9 2,098,463 + -2.7 2,098,501 - +2.1 2,098,524 - +1.7 2,098,549 - +1.0 2,098,551 + -0.4 2,098,556 + -0.9 2,098,556 + +0.7 2,098,570 - +0.3 2,098,579 + +0.7 2,098,604 + +0.9 2,098,628 + +0.4 2,098,628 - -0.3 2,098,638 + -0.7 2,098,638 - -1.0 2,098,646 - +0.4 2,098,697 - +0.3 2,098,727 - +0.3 2,098,736 - +0.4 2,098,758 + GFF2061 0.11 +0.7 2,098,891 + GFF2061 0.21 -0.8 2,098,904 - GFF2061 0.22 +0.1 2,098,928 + GFF2061 0.23 -0.8 2,098,931 - GFF2061 0.24 +0.4 2,098,936 - GFF2061 0.24 -1.0 2,098,988 + GFF2061 0.28 +0.3 2,098,996 - GFF2061 0.28 -1.4 2,099,015 + GFF2061 0.30 +0.8 2,099,022 + GFF2061 0.30 -1.5 2,099,022 + GFF2061 0.30 -0.8 2,099,060 + GFF2061 0.33 -0.2 2,099,068 - GFF2061 0.34 -0.6 2,099,108 - GFF2061 0.37 -0.3 2,099,118 - GFF2061 0.37 +1.0 2,099,129 + GFF2061 0.38 -0.2 2,099,129 + GFF2061 0.38 +0.3 2,099,138 + GFF2061 0.39 +1.1 2,099,192 + GFF2061 0.43 +1.1 2,099,192 + GFF2061 0.43 +0.6 2,099,192 + GFF2061 0.43 -0.8 2,099,200 - GFF2061 0.43 +1.0 2,099,200 - GFF2061 0.43 +0.3 2,099,224 + GFF2061 0.45 -0.1 2,099,239 - GFF2061 0.46 +0.2 2,099,257 - GFF2061 0.47 +0.0 2,099,259 + GFF2061 0.47 +0.1 2,099,311 + GFF2061 0.51 -0.2 2,099,372 + GFF2061 0.56 +0.7 2,099,419 - GFF2061 0.59 +0.6 2,099,433 + GFF2061 0.60 +0.0 2,099,440 - GFF2061 0.61 -0.7 2,099,440 - GFF2061 0.61 +0.5 2,099,440 - GFF2061 0.61 -2.6 2,099,441 - GFF2061 0.61 -0.5 2,099,462 - GFF2061 0.62 +0.5 2,099,462 - GFF2061 0.62 -0.2 2,099,476 - GFF2061 0.63 +1.9 2,099,494 + GFF2061 0.64 +1.4 2,099,519 + GFF2061 0.66 -1.0 2,099,525 - GFF2061 0.67 -0.8 2,099,527 - GFF2061 0.67 -0.1 2,099,527 - GFF2061 0.67 +0.0 2,099,527 - GFF2061 0.67 -0.6 2,099,539 - GFF2061 0.68 +0.8 2,099,583 + GFF2061 0.71 -0.2 2,099,583 + GFF2061 0.71 -1.8 2,099,697 + GFF2061 0.79 -1.1 2,099,697 + GFF2061 0.79 -0.3 2,099,822 + GFF2061 0.88 +1.0 2,099,824 + GFF2061 0.88 -0.6 2,099,835 + GFF2061 0.89 +1.3 2,099,851 - -1.0 2,099,862 + -2.8 2,099,924 + +0.3 2,099,932 - +0.3 2,099,950 - -1.1 2,099,950 - +0.3 2,099,995 - -0.1 2,100,084 - -0.8 2,100,089 - +0.2 2,100,090 + -0.4 2,100,090 + -1.8 2,100,090 + +0.4 2,100,098 - +0.4 2,100,099 - -1.8 2,100,141 + -1.8 2,100,151 - +0.6 2,100,199 + +0.9 2,100,228 + -0.1 2,100,281 - +0.5 2,100,307 + +1.4 2,100,308 + -0.3 2,100,308 + -0.3 2,100,365 + GFF2062 0.14 -0.1 2,100,373 - GFF2062 0.15 +2.1 2,100,410 + GFF2062 0.18 +0.0 2,100,410 + GFF2062 0.18 -0.4 2,100,418 - GFF2062 0.18 -0.3 2,100,440 - GFF2062 0.20 +0.3 2,100,443 + GFF2062 0.20 +0.2 2,100,443 + GFF2062 0.20 +0.0 2,100,486 - GFF2062 0.24 +2.0 2,100,486 - GFF2062 0.24 -1.0 2,100,486 - GFF2062 0.24 -0.1 2,100,519 + GFF2062 0.26 +0.8 2,100,519 + GFF2062 0.26 -0.2 2,100,519 + GFF2062 0.26 +0.8 2,100,519 + GFF2062 0.26 +0.6 2,100,527 - GFF2062 0.27 +0.1 2,100,527 - GFF2062 0.27 -1.0 2,100,529 + GFF2062 0.27 +0.0 2,100,532 - GFF2062 0.27 +0.5 2,100,588 + GFF2062 0.31 -1.0 2,100,588 + GFF2062 0.31 +1.5 2,100,614 - GFF2062 0.33 -0.0 2,100,709 + GFF2062 0.41 -2.1 2,100,734 + GFF2062 0.43 -0.7 2,100,743 - GFF2062 0.43 -0.2 2,100,786 + GFF2062 0.47 +0.3 2,100,786 + GFF2062 0.47 +0.3 2,100,786 + GFF2062 0.47 -0.6 2,100,786 + GFF2062 0.47 +0.4 2,100,786 + GFF2062 0.47 -0.1 2,100,786 + GFF2062 0.47 -0.6 2,100,794 - GFF2062 0.47 -0.1 2,100,794 - GFF2062 0.47 +0.5 2,100,794 - GFF2062 0.47 +0.5 2,100,794 - GFF2062 0.47 -1.0 2,100,794 - GFF2062 0.47 +0.9 2,100,794 - GFF2062 0.47 +0.0 2,100,794 - GFF2062 0.47 -0.4 2,100,794 - GFF2062 0.47 -0.6 2,100,794 - GFF2062 0.47 +1.0 2,100,794 - GFF2062 0.47 +1.1 2,100,794 - GFF2062 0.47 -0.1 2,100,794 - GFF2062 0.47 -0.0 2,100,794 - GFF2062 0.47 -0.5 2,100,794 - GFF2062 0.47 +0.5 2,100,796 + GFF2062 0.47 +0.8 2,100,799 - GFF2062 0.48 -1.2 2,100,799 - GFF2062 0.48 +0.3 2,100,805 - GFF2062 0.48 -1.8 2,100,853 + GFF2062 0.52 +0.4 2,100,861 - GFF2062 0.52 +0.0 2,100,878 + GFF2062 0.54 -0.5 2,100,878 + GFF2062 0.54 -0.1 2,100,886 - GFF2062 0.54 -0.3 2,100,894 - GFF2062 0.55 +1.2 2,100,904 + GFF2062 0.56 +1.2 2,100,909 + GFF2062 0.56 -1.2 2,100,955 + GFF2062 0.60 +1.6 2,100,970 - GFF2062 0.61 +1.0
Or see this region's nucleotide sequence