Strain Fitness in Sphingobium sp. HT1-2 around GFF1997

Experiment: EMM_noCarbon with D-Glucose and Ammonium Sulfate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF1995 and GFF1996 are separated by 94 nucleotidesGFF1996 and GFF1997 are separated by 75 nucleotidesGFF1997 and GFF1998 are separated by 1 nucleotidesGFF1998 and GFF1999 are separated by 92 nucleotides GFF1995 - sigma-54 dependent DNA-binding response regulator, at 2,015,371 to 2,016,807 GFF1995 GFF1996 - hypothetical protein, at 2,016,902 to 2,017,537 GFF1996 GFF1997 - Acetyltransferase, GNAT family, at 2,017,613 to 2,018,113 GFF1997 GFF1998 - hypothetical protein, at 2,018,115 to 2,018,633 GFF1998 GFF1999 - Sun protein, at 2,018,726 to 2,019,901 GFF1999 Position (kb) 2017 2018 2019Strain fitness (log2 ratio) -2 -1 0 1 2at 2016.626 kb on + strand, within GFF1995at 2016.626 kb on + strand, within GFF1995at 2016.626 kb on + strand, within GFF1995at 2016.626 kb on + strand, within GFF1995at 2016.626 kb on + strand, within GFF1995at 2016.634 kb on - strand, within GFF1995at 2016.634 kb on - strand, within GFF1995at 2016.636 kb on + strand, within GFF1995at 2016.636 kb on + strand, within GFF1995at 2016.644 kb on - strand, within GFF1995at 2016.651 kb on - strand, within GFF1995at 2016.666 kb on + strandat 2016.745 kb on + strandat 2016.795 kb on - strandat 2016.863 kb on - strandat 2016.871 kb on - strandat 2016.888 kb on - strandat 2016.909 kb on + strandat 2016.950 kb on + strandat 2017.003 kb on - strand, within GFF1996at 2017.016 kb on - strand, within GFF1996at 2017.036 kb on + strand, within GFF1996at 2017.039 kb on - strand, within GFF1996at 2017.130 kb on - strand, within GFF1996at 2017.229 kb on + strand, within GFF1996at 2017.260 kb on - strand, within GFF1996at 2017.336 kb on - strand, within GFF1996at 2017.394 kb on - strand, within GFF1996at 2017.443 kb on - strand, within GFF1996at 2017.447 kb on - strand, within GFF1996at 2017.447 kb on - strand, within GFF1996at 2017.449 kb on - strand, within GFF1996at 2017.451 kb on + strand, within GFF1996at 2017.590 kb on - strandat 2017.603 kb on + strandat 2017.603 kb on + strandat 2017.603 kb on + strandat 2017.603 kb on + strandat 2017.603 kb on + strandat 2017.611 kb on - strandat 2017.616 kb on + strandat 2017.621 kb on + strandat 2017.662 kb on + strandat 2017.664 kb on - strand, within GFF1997at 2017.727 kb on - strand, within GFF1997at 2017.727 kb on - strand, within GFF1997at 2017.727 kb on - strand, within GFF1997at 2017.727 kb on - strand, within GFF1997at 2017.737 kb on - strand, within GFF1997at 2017.773 kb on + strand, within GFF1997at 2017.839 kb on - strand, within GFF1997at 2017.839 kb on - strand, within GFF1997at 2017.854 kb on - strand, within GFF1997at 2017.897 kb on - strand, within GFF1997at 2017.913 kb on + strand, within GFF1997at 2017.918 kb on + strand, within GFF1997at 2017.926 kb on - strand, within GFF1997at 2018.113 kb on + strandat 2018.139 kb on - strandat 2018.203 kb on + strand, within GFF1998at 2018.203 kb on + strand, within GFF1998at 2018.203 kb on + strand, within GFF1998at 2018.203 kb on + strand, within GFF1998at 2018.211 kb on - strand, within GFF1998at 2018.211 kb on - strand, within GFF1998at 2018.233 kb on + strand, within GFF1998at 2018.233 kb on + strand, within GFF1998at 2018.233 kb on + strand, within GFF1998at 2018.241 kb on - strand, within GFF1998at 2018.289 kb on - strand, within GFF1998at 2018.302 kb on + strand, within GFF1998at 2018.382 kb on - strand, within GFF1998at 2018.382 kb on - strand, within GFF1998at 2018.412 kb on - strand, within GFF1998at 2018.412 kb on - strand, within GFF1998at 2018.422 kb on - strand, within GFF1998at 2018.449 kb on + strand, within GFF1998at 2018.489 kb on - strand, within GFF1998at 2018.549 kb on + strand, within GFF1998at 2018.564 kb on + strand, within GFF1998at 2018.573 kb on - strand, within GFF1998at 2018.589 kb on + strandat 2018.623 kb on - strandat 2018.624 kb on - strandat 2018.656 kb on + strandat 2018.657 kb on - strandat 2018.671 kb on - strandat 2018.691 kb on - strandat 2018.707 kb on + strandat 2018.733 kb on + strandat 2018.813 kb on + strandat 2018.813 kb on + strandat 2018.813 kb on + strandat 2018.821 kb on - strandat 2018.921 kb on - strand, within GFF1999at 2018.926 kb on - strand, within GFF1999at 2018.969 kb on + strand, within GFF1999at 2019.005 kb on + strand, within GFF1999at 2019.022 kb on + strand, within GFF1999at 2019.049 kb on + strand, within GFF1999at 2019.057 kb on - strand, within GFF1999

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Per-strain Table

Position Strand Gene LocusTag Fraction EMM_noCarbon with D-Glucose and Ammonium Sulfate
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2,016,626 + GFF1995 0.87 -0.5
2,016,626 + GFF1995 0.87 +0.1
2,016,626 + GFF1995 0.87 +0.7
2,016,626 + GFF1995 0.87 -0.7
2,016,626 + GFF1995 0.87 -0.3
2,016,634 - GFF1995 0.88 +1.1
2,016,634 - GFF1995 0.88 +0.3
2,016,636 + GFF1995 0.88 -0.1
2,016,636 + GFF1995 0.88 -1.7
2,016,644 - GFF1995 0.89 +1.2
2,016,651 - GFF1995 0.89 +0.1
2,016,666 + +1.6
2,016,745 + +0.7
2,016,795 - -0.1
2,016,863 - +1.4
2,016,871 - -0.4
2,016,888 - +0.2
2,016,909 + -2.4
2,016,950 + -0.4
2,017,003 - GFF1996 0.16 -0.7
2,017,016 - GFF1996 0.18 +1.1
2,017,036 + GFF1996 0.21 -0.3
2,017,039 - GFF1996 0.22 -0.3
2,017,130 - GFF1996 0.36 -0.7
2,017,229 + GFF1996 0.51 +1.0
2,017,260 - GFF1996 0.56 +0.8
2,017,336 - GFF1996 0.68 +0.1
2,017,394 - GFF1996 0.77 -0.5
2,017,443 - GFF1996 0.85 -0.6
2,017,447 - GFF1996 0.86 +1.1
2,017,447 - GFF1996 0.86 +1.3
2,017,449 - GFF1996 0.86 -1.4
2,017,451 + GFF1996 0.86 +0.9
2,017,590 - -0.6
2,017,603 + -0.4
2,017,603 + +0.9
2,017,603 + -0.8
2,017,603 + +0.1
2,017,603 + -0.5
2,017,611 - -1.4
2,017,616 + +0.8
2,017,621 + +1.5
2,017,662 + -1.7
2,017,664 - GFF1997 0.10 +0.6
2,017,727 - GFF1997 0.23 -0.3
2,017,727 - GFF1997 0.23 -0.1
2,017,727 - GFF1997 0.23 +0.4
2,017,727 - GFF1997 0.23 +0.4
2,017,737 - GFF1997 0.25 -0.1
2,017,773 + GFF1997 0.32 -2.7
2,017,839 - GFF1997 0.45 -0.1
2,017,839 - GFF1997 0.45 +0.7
2,017,854 - GFF1997 0.48 -1.6
2,017,897 - GFF1997 0.57 +0.4
2,017,913 + GFF1997 0.60 +1.6
2,017,918 + GFF1997 0.61 -0.6
2,017,926 - GFF1997 0.62 -0.4
2,018,113 + +0.5
2,018,139 - -0.1
2,018,203 + GFF1998 0.17 +0.5
2,018,203 + GFF1998 0.17 +0.8
2,018,203 + GFF1998 0.17 +1.2
2,018,203 + GFF1998 0.17 +0.5
2,018,211 - GFF1998 0.18 -0.9
2,018,211 - GFF1998 0.18 +0.0
2,018,233 + GFF1998 0.23 -1.3
2,018,233 + GFF1998 0.23 +0.1
2,018,233 + GFF1998 0.23 +0.1
2,018,241 - GFF1998 0.24 +0.1
2,018,289 - GFF1998 0.34 +1.2
2,018,302 + GFF1998 0.36 -0.8
2,018,382 - GFF1998 0.51 +0.0
2,018,382 - GFF1998 0.51 +1.5
2,018,412 - GFF1998 0.57 +0.8
2,018,412 - GFF1998 0.57 -0.2
2,018,422 - GFF1998 0.59 +0.7
2,018,449 + GFF1998 0.64 -0.6
2,018,489 - GFF1998 0.72 +0.4
2,018,549 + GFF1998 0.84 +0.4
2,018,564 + GFF1998 0.87 +2.4
2,018,573 - GFF1998 0.88 -1.0
2,018,589 + +0.0
2,018,623 - -2.7
2,018,624 - -2.7
2,018,656 + -0.9
2,018,657 - +0.4
2,018,671 - -2.0
2,018,691 - -0.2
2,018,707 + +1.5
2,018,733 + +0.6
2,018,813 + -1.1
2,018,813 + +1.3
2,018,813 + +0.7
2,018,821 - -0.1
2,018,921 - GFF1999 0.17 +0.5
2,018,926 - GFF1999 0.17 +0.4
2,018,969 + GFF1999 0.21 -0.1
2,019,005 + GFF1999 0.24 -1.5
2,019,022 + GFF1999 0.25 -1.6
2,019,049 + GFF1999 0.27 +0.0
2,019,057 - GFF1999 0.28 -1.8

Or see this region's nucleotide sequence