Experiment: EMM_noCarbon with D-Glucose and Ammonium Sulfate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF179 and GFF180 overlap by 11 nucleotides GFF180 and GFF181 are separated by 233 nucleotides GFF181 and GFF182 are separated by 265 nucleotides
GFF178 - Periplasmic chaperone and peptidyl-prolyl cis-trans isomerase of outer membrane proteins SurA (EC 5.2.1.8), at 181,236 to 182,600
GFF178
GFF179 - 4-hydroxythreonine-4-phosphate dehydrogenase (EC 1.1.1.262), at 182,578 to 183,603
GFF179
GFF180 - SSU rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase (EC 2.1.1.182), at 183,593 to 184,447
GFF180
GFF181 - hypothetical protein, at 184,681 to 184,911
GFF181
GFF182 - hypothetical protein, at 185,177 to 185,659
GFF182
Position (kb)
183
184
185 Strain fitness (log2 ratio)
-2
-1
0
1 at 183.591 kb on - strand at 183.613 kb on + strand at 183.658 kb on + strand at 183.784 kb on - strand, within GFF180 at 183.784 kb on - strand, within GFF180 at 183.871 kb on - strand, within GFF180 at 183.878 kb on + strand, within GFF180 at 183.978 kb on + strand, within GFF180 at 184.019 kb on + strand, within GFF180 at 184.032 kb on - strand, within GFF180 at 184.046 kb on - strand, within GFF180 at 184.087 kb on + strand, within GFF180 at 184.246 kb on + strand, within GFF180 at 184.246 kb on + strand, within GFF180 at 184.254 kb on - strand, within GFF180 at 184.278 kb on + strand, within GFF180 at 184.341 kb on + strand, within GFF180 at 184.364 kb on - strand at 184.509 kb on - strand at 184.534 kb on + strand at 184.534 kb on - strand at 184.547 kb on - strand at 184.616 kb on + strand at 184.646 kb on - strand at 184.665 kb on - strand at 184.665 kb on - strand at 184.741 kb on + strand, within GFF181 at 184.755 kb on + strand, within GFF181 at 184.755 kb on + strand, within GFF181 at 184.791 kb on - strand, within GFF181 at 184.806 kb on + strand, within GFF181 at 184.854 kb on + strand, within GFF181 at 184.864 kb on + strand, within GFF181 at 184.866 kb on - strand, within GFF181 at 184.880 kb on + strand, within GFF181 at 184.883 kb on - strand, within GFF181 at 184.883 kb on - strand, within GFF181 at 184.885 kb on + strand, within GFF181 at 184.885 kb on + strand, within GFF181 at 184.885 kb on + strand, within GFF181 at 184.885 kb on + strand, within GFF181 at 184.888 kb on + strand, within GFF181 at 184.888 kb on - strand, within GFF181 at 184.890 kb on + strand at 184.890 kb on + strand at 184.893 kb on - strand at 184.910 kb on + strand at 184.910 kb on + strand at 184.910 kb on + strand at 184.910 kb on + strand at 184.910 kb on + strand at 184.910 kb on + strand at 184.910 kb on + strand at 184.910 kb on + strand at 184.910 kb on + strand at 184.910 kb on + strand at 184.910 kb on + strand at 184.915 kb on + strand at 184.915 kb on + strand at 184.915 kb on + strand at 184.918 kb on - strand at 184.918 kb on - strand at 184.918 kb on - strand at 184.923 kb on - strand at 184.923 kb on - strand at 184.928 kb on + strand at 184.928 kb on + strand at 184.928 kb on + strand at 184.936 kb on - strand at 184.958 kb on - strand at 184.973 kb on + strand at 184.981 kb on + strand at 184.985 kb on + strand at 184.999 kb on - strand at 185.015 kb on - strand at 185.030 kb on + strand at 185.030 kb on + strand at 185.030 kb on + strand at 185.030 kb on + strand at 185.035 kb on + strand at 185.035 kb on + strand at 185.035 kb on + strand at 185.038 kb on - strand at 185.038 kb on - strand at 185.038 kb on - strand at 185.040 kb on + strand at 185.040 kb on + strand at 185.040 kb on + strand at 185.040 kb on + strand at 185.040 kb on + strand at 185.040 kb on + strand at 185.040 kb on + strand at 185.040 kb on + strand at 185.043 kb on - strand at 185.043 kb on - strand at 185.043 kb on - strand at 185.048 kb on - strand at 185.048 kb on - strand at 185.048 kb on - strand at 185.048 kb on - strand at 185.048 kb on - strand at 185.048 kb on - strand at 185.048 kb on - strand at 185.048 kb on - strand at 185.069 kb on + strand at 185.077 kb on + strand at 185.077 kb on - strand at 185.116 kb on - strand at 185.118 kb on + strand at 185.120 kb on + strand at 185.128 kb on - strand at 185.128 kb on - strand at 185.135 kb on - strand at 185.135 kb on - strand at 185.135 kb on - strand at 185.153 kb on + strand at 185.153 kb on + strand at 185.153 kb on + strand at 185.161 kb on - strand at 185.161 kb on - strand at 185.161 kb on - strand at 185.161 kb on - strand at 185.161 kb on - strand at 185.197 kb on - strand at 185.209 kb on + strand at 185.214 kb on + strand at 185.219 kb on + strand at 185.219 kb on + strand at 185.222 kb on - strand at 185.227 kb on - strand, within GFF182 at 185.246 kb on - strand, within GFF182 at 185.258 kb on - strand, within GFF182 at 185.262 kb on + strand, within GFF182 at 185.277 kb on - strand, within GFF182 at 185.316 kb on + strand, within GFF182 at 185.330 kb on + strand, within GFF182 at 185.348 kb on + strand, within GFF182 at 185.360 kb on - strand, within GFF182 at 185.361 kb on + strand, within GFF182 at 185.361 kb on + strand, within GFF182 at 185.361 kb on + strand, within GFF182 at 185.379 kb on - strand, within GFF182 at 185.425 kb on - strand, within GFF182
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with D-Glucose and Ammonium Sulfate remove 183,591 - +0.1 183,613 + -0.4 183,658 + -0.8 183,784 - GFF180 0.22 -0.1 183,784 - GFF180 0.22 -2.3 183,871 - GFF180 0.33 -0.6 183,878 + GFF180 0.33 -0.9 183,978 + GFF180 0.45 +0.2 184,019 + GFF180 0.50 -0.3 184,032 - GFF180 0.51 -0.6 184,046 - GFF180 0.53 -1.0 184,087 + GFF180 0.58 -0.3 184,246 + GFF180 0.76 +0.2 184,246 + GFF180 0.76 +0.2 184,254 - GFF180 0.77 -1.4 184,278 + GFF180 0.80 +0.5 184,341 + GFF180 0.87 -0.5 184,364 - -0.5 184,509 - +0.7 184,534 + +0.1 184,534 - +0.1 184,547 - -0.6 184,616 + -0.0 184,646 - -1.4 184,665 - -0.4 184,665 - +0.2 184,741 + GFF181 0.26 -1.5 184,755 + GFF181 0.32 +0.1 184,755 + GFF181 0.32 +0.1 184,791 - GFF181 0.48 -0.1 184,806 + GFF181 0.54 -0.7 184,854 + GFF181 0.75 -0.5 184,864 + GFF181 0.79 +0.9 184,866 - GFF181 0.80 +0.7 184,880 + GFF181 0.86 +0.2 184,883 - GFF181 0.87 -0.1 184,883 - GFF181 0.87 +0.8 184,885 + GFF181 0.88 -0.8 184,885 + GFF181 0.88 -0.1 184,885 + GFF181 0.88 +0.7 184,885 + GFF181 0.88 +1.5 184,888 + GFF181 0.90 -1.2 184,888 - GFF181 0.90 +0.6 184,890 + +0.5 184,890 + +1.4 184,893 - +0.7 184,910 + +0.5 184,910 + +1.3 184,910 + -0.9 184,910 + +0.3 184,910 + +0.8 184,910 + +0.2 184,910 + -0.1 184,910 + -1.4 184,910 + +0.1 184,910 + +0.3 184,910 + -0.2 184,915 + -0.2 184,915 + -1.0 184,915 + +0.4 184,918 - -0.7 184,918 - +0.3 184,918 - +0.5 184,923 - +0.7 184,923 - +0.4 184,928 + -0.9 184,928 + +0.5 184,928 + +0.7 184,936 - -0.1 184,958 - +0.0 184,973 + -0.3 184,981 + +0.4 184,985 + -0.2 184,999 - +0.5 185,015 - +0.7 185,030 + +1.0 185,030 + +0.4 185,030 + +0.1 185,030 + +0.7 185,035 + +0.6 185,035 + +0.2 185,035 + -0.8 185,038 - -1.2 185,038 - -0.7 185,038 - -0.1 185,040 + +0.5 185,040 + -0.8 185,040 + +0.4 185,040 + +0.8 185,040 + -0.2 185,040 + -0.3 185,040 + -0.2 185,040 + +0.6 185,043 - -2.1 185,043 - -0.4 185,043 - +0.1 185,048 - +0.2 185,048 - +1.0 185,048 - +1.1 185,048 - +0.9 185,048 - -0.7 185,048 - -0.5 185,048 - -0.5 185,048 - +0.8 185,069 + -0.2 185,077 + -0.6 185,077 - +1.4 185,116 - -0.9 185,118 + +0.2 185,120 + +0.8 185,128 - -1.2 185,128 - +0.1 185,135 - +0.6 185,135 - -1.3 185,135 - +0.9 185,153 + -0.6 185,153 + -1.8 185,153 + -0.7 185,161 - -0.2 185,161 - -0.3 185,161 - +0.4 185,161 - -0.0 185,161 - -0.1 185,197 - +0.3 185,209 + +0.7 185,214 + +0.4 185,219 + -0.7 185,219 + +0.7 185,222 - -0.1 185,227 - GFF182 0.10 +0.4 185,246 - GFF182 0.14 -0.5 185,258 - GFF182 0.17 +0.1 185,262 + GFF182 0.18 -0.0 185,277 - GFF182 0.21 -0.4 185,316 + GFF182 0.29 -0.5 185,330 + GFF182 0.32 -0.7 185,348 + GFF182 0.35 +0.2 185,360 - GFF182 0.38 +0.8 185,361 + GFF182 0.38 -0.3 185,361 + GFF182 0.38 -0.2 185,361 + GFF182 0.38 +0.1 185,379 - GFF182 0.42 +0.4 185,425 - GFF182 0.51 +1.2
Or see this region's nucleotide sequence