Experiment: EMM_noCarbon with D-Glucose and Ammonium Sulfate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1594 and GFF1595 overlap by 4 nucleotides GFF1595 and GFF1596 are separated by 56 nucleotides GFF1596 and GFF1597 are separated by 27 nucleotides GFF1597 and GFF1598 overlap by 4 nucleotides GFF1598 and GFF1599 overlap by 4 nucleotides
GFF1594 - Glucokinase (EC 2.7.1.2), at 1,622,138 to 1,623,118
GFF1594
GFF1595 - 4-hydroxy-2-oxoglutarate aldolase (EC 4.1.3.16) @ 2-dehydro-3-deoxyphosphogluconate aldolase (EC 4.1.2.14), at 1,623,115 to 1,623,732
GFF1595
GFF1596 - hypothetical protein, at 1,623,789 to 1,624,310
GFF1596
GFF1597 - hypothetical protein, at 1,624,338 to 1,624,757
GFF1597
GFF1598 - hypothetical protein, at 1,624,754 to 1,625,269
GFF1598
GFF1599 - hypothetical protein, at 1,625,266 to 1,627,284
GFF1599
Position (kb)
1623
1624
1625 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1622.883 kb on + strand, within GFF1594 at 1622.883 kb on + strand, within GFF1594 at 1622.891 kb on - strand, within GFF1594 at 1622.891 kb on - strand, within GFF1594 at 1622.922 kb on + strand, within GFF1594 at 1622.930 kb on - strand, within GFF1594 at 1622.933 kb on + strand, within GFF1594 at 1622.949 kb on + strand, within GFF1594 at 1622.983 kb on + strand, within GFF1594 at 1623.009 kb on - strand, within GFF1594 at 1623.087 kb on - strand at 1623.087 kb on - strand at 1623.089 kb on + strand at 1623.118 kb on + strand at 1623.213 kb on + strand, within GFF1595 at 1623.745 kb on + strand at 1623.751 kb on + strand at 1623.753 kb on - strand at 1623.765 kb on - strand at 1623.816 kb on + strand at 1623.823 kb on + strand at 1623.824 kb on - strand at 1623.837 kb on + strand at 1623.858 kb on + strand, within GFF1596 at 1623.858 kb on + strand, within GFF1596 at 1623.858 kb on + strand, within GFF1596 at 1623.858 kb on + strand, within GFF1596 at 1623.858 kb on + strand, within GFF1596 at 1623.858 kb on + strand, within GFF1596 at 1623.858 kb on + strand, within GFF1596 at 1623.864 kb on + strand, within GFF1596 at 1623.866 kb on - strand, within GFF1596 at 1623.866 kb on - strand, within GFF1596 at 1623.886 kb on + strand, within GFF1596 at 1623.891 kb on + strand, within GFF1596 at 1623.892 kb on - strand, within GFF1596 at 1623.960 kb on + strand, within GFF1596 at 1623.960 kb on + strand, within GFF1596 at 1623.960 kb on + strand, within GFF1596 at 1623.960 kb on + strand, within GFF1596 at 1623.965 kb on + strand, within GFF1596 at 1623.968 kb on - strand, within GFF1596 at 1623.982 kb on + strand, within GFF1596 at 1623.987 kb on + strand, within GFF1596 at 1623.987 kb on + strand, within GFF1596 at 1624.016 kb on + strand, within GFF1596 at 1624.016 kb on + strand, within GFF1596 at 1624.017 kb on + strand, within GFF1596 at 1624.017 kb on + strand, within GFF1596 at 1624.017 kb on + strand, within GFF1596 at 1624.017 kb on + strand, within GFF1596 at 1624.017 kb on + strand, within GFF1596 at 1624.017 kb on + strand, within GFF1596 at 1624.017 kb on + strand, within GFF1596 at 1624.017 kb on + strand, within GFF1596 at 1624.017 kb on + strand, within GFF1596 at 1624.017 kb on + strand, within GFF1596 at 1624.017 kb on + strand, within GFF1596 at 1624.017 kb on + strand, within GFF1596 at 1624.017 kb on + strand, within GFF1596 at 1624.023 kb on - strand, within GFF1596 at 1624.024 kb on - strand, within GFF1596 at 1624.024 kb on - strand, within GFF1596 at 1624.024 kb on - strand, within GFF1596 at 1624.025 kb on - strand, within GFF1596 at 1624.025 kb on - strand, within GFF1596 at 1624.025 kb on - strand, within GFF1596 at 1624.025 kb on - strand, within GFF1596 at 1624.025 kb on - strand, within GFF1596 at 1624.025 kb on - strand, within GFF1596 at 1624.025 kb on - strand, within GFF1596 at 1624.025 kb on - strand, within GFF1596 at 1624.025 kb on - strand, within GFF1596 at 1624.025 kb on - strand, within GFF1596 at 1624.025 kb on - strand, within GFF1596 at 1624.025 kb on - strand, within GFF1596 at 1624.025 kb on - strand, within GFF1596 at 1624.025 kb on - strand, within GFF1596 at 1624.046 kb on - strand, within GFF1596 at 1624.065 kb on + strand, within GFF1596 at 1624.070 kb on + strand, within GFF1596 at 1624.140 kb on + strand, within GFF1596 at 1624.151 kb on + strand, within GFF1596 at 1624.183 kb on - strand, within GFF1596 at 1624.200 kb on + strand, within GFF1596 at 1624.213 kb on - strand, within GFF1596 at 1624.247 kb on + strand, within GFF1596 at 1624.255 kb on - strand, within GFF1596 at 1624.298 kb on + strand at 1624.299 kb on + strand at 1624.299 kb on + strand at 1624.299 kb on + strand at 1624.299 kb on + strand at 1624.299 kb on + strand at 1624.299 kb on + strand at 1624.299 kb on + strand at 1624.304 kb on + strand at 1624.304 kb on + strand at 1624.304 kb on + strand at 1624.304 kb on + strand at 1624.307 kb on - strand at 1624.307 kb on - strand at 1624.307 kb on - strand at 1624.341 kb on + strand at 1624.378 kb on + strand at 1624.386 kb on - strand, within GFF1597 at 1624.428 kb on - strand, within GFF1597 at 1624.438 kb on + strand, within GFF1597 at 1624.438 kb on + strand, within GFF1597 at 1624.438 kb on + strand, within GFF1597 at 1624.446 kb on - strand, within GFF1597 at 1624.446 kb on - strand, within GFF1597 at 1624.446 kb on - strand, within GFF1597 at 1624.446 kb on - strand, within GFF1597 at 1624.448 kb on + strand, within GFF1597 at 1624.451 kb on - strand, within GFF1597 at 1624.451 kb on - strand, within GFF1597 at 1624.456 kb on - strand, within GFF1597 at 1624.456 kb on - strand, within GFF1597 at 1624.468 kb on + strand, within GFF1597 at 1624.468 kb on + strand, within GFF1597 at 1624.473 kb on + strand, within GFF1597 at 1624.473 kb on + strand, within GFF1597 at 1624.476 kb on - strand, within GFF1597 at 1624.476 kb on - strand, within GFF1597 at 1624.481 kb on - strand, within GFF1597 at 1624.516 kb on + strand, within GFF1597 at 1624.521 kb on + strand, within GFF1597 at 1624.532 kb on + strand, within GFF1597 at 1624.534 kb on + strand, within GFF1597 at 1624.539 kb on + strand, within GFF1597 at 1624.540 kb on - strand, within GFF1597 at 1624.542 kb on - strand, within GFF1597 at 1624.545 kb on - strand, within GFF1597 at 1624.547 kb on + strand, within GFF1597 at 1624.561 kb on - strand, within GFF1597 at 1624.572 kb on + strand, within GFF1597 at 1624.580 kb on - strand, within GFF1597 at 1624.580 kb on - strand, within GFF1597 at 1624.580 kb on - strand, within GFF1597 at 1624.588 kb on - strand, within GFF1597 at 1624.588 kb on - strand, within GFF1597 at 1624.597 kb on + strand, within GFF1597 at 1624.600 kb on - strand, within GFF1597 at 1624.604 kb on + strand, within GFF1597 at 1624.610 kb on - strand, within GFF1597 at 1624.619 kb on + strand, within GFF1597 at 1624.630 kb on + strand, within GFF1597 at 1624.630 kb on + strand, within GFF1597 at 1624.630 kb on + strand, within GFF1597 at 1624.630 kb on + strand, within GFF1597 at 1624.630 kb on + strand, within GFF1597 at 1624.635 kb on + strand, within GFF1597 at 1624.638 kb on - strand, within GFF1597 at 1624.638 kb on - strand, within GFF1597 at 1624.643 kb on + strand, within GFF1597 at 1624.643 kb on + strand, within GFF1597 at 1624.651 kb on - strand, within GFF1597 at 1624.683 kb on - strand, within GFF1597 at 1624.688 kb on - strand, within GFF1597 at 1624.719 kb on + strand at 1624.727 kb on - strand at 1624.727 kb on - strand at 1624.727 kb on - strand at 1624.741 kb on - strand at 1624.745 kb on + strand at 1624.747 kb on - strand at 1624.752 kb on + strand at 1624.752 kb on + strand at 1624.775 kb on + strand at 1624.778 kb on - strand at 1624.778 kb on - strand at 1624.816 kb on - strand, within GFF1598 at 1624.856 kb on + strand, within GFF1598 at 1624.858 kb on - strand, within GFF1598 at 1624.893 kb on + strand, within GFF1598 at 1624.893 kb on + strand, within GFF1598 at 1624.898 kb on + strand, within GFF1598 at 1624.898 kb on + strand, within GFF1598 at 1624.906 kb on - strand, within GFF1598 at 1624.906 kb on - strand, within GFF1598 at 1624.911 kb on - strand, within GFF1598 at 1624.929 kb on - strand, within GFF1598 at 1624.929 kb on - strand, within GFF1598 at 1624.929 kb on - strand, within GFF1598 at 1624.929 kb on - strand, within GFF1598 at 1624.929 kb on - strand, within GFF1598 at 1624.936 kb on - strand, within GFF1598 at 1624.945 kb on + strand, within GFF1598 at 1624.947 kb on - strand, within GFF1598 at 1624.965 kb on - strand, within GFF1598 at 1624.965 kb on - strand, within GFF1598 at 1624.982 kb on + strand, within GFF1598 at 1624.994 kb on - strand, within GFF1598 at 1625.010 kb on - strand, within GFF1598 at 1625.026 kb on - strand, within GFF1598 at 1625.040 kb on - strand, within GFF1598 at 1625.042 kb on + strand, within GFF1598 at 1625.042 kb on + strand, within GFF1598 at 1625.066 kb on - strand, within GFF1598 at 1625.072 kb on - strand, within GFF1598 at 1625.088 kb on + strand, within GFF1598 at 1625.090 kb on + strand, within GFF1598 at 1625.090 kb on + strand, within GFF1598 at 1625.104 kb on + strand, within GFF1598 at 1625.112 kb on + strand, within GFF1598 at 1625.112 kb on - strand, within GFF1598 at 1625.112 kb on - strand, within GFF1598 at 1625.115 kb on - strand, within GFF1598 at 1625.141 kb on - strand, within GFF1598 at 1625.145 kb on + strand, within GFF1598 at 1625.153 kb on - strand, within GFF1598 at 1625.155 kb on + strand, within GFF1598 at 1625.176 kb on + strand, within GFF1598 at 1625.178 kb on - strand, within GFF1598 at 1625.184 kb on + strand, within GFF1598 at 1625.189 kb on + strand, within GFF1598 at 1625.192 kb on - strand, within GFF1598 at 1625.192 kb on - strand, within GFF1598 at 1625.192 kb on - strand, within GFF1598 at 1625.192 kb on - strand, within GFF1598 at 1625.194 kb on + strand, within GFF1598 at 1625.194 kb on + strand, within GFF1598 at 1625.200 kb on + strand, within GFF1598 at 1625.202 kb on - strand, within GFF1598 at 1625.202 kb on - strand, within GFF1598 at 1625.228 kb on + strand at 1625.233 kb on + strand at 1625.233 kb on + strand at 1625.236 kb on - strand at 1625.241 kb on - strand at 1625.241 kb on - strand at 1625.241 kb on - strand at 1625.241 kb on - strand at 1625.246 kb on - strand at 1625.272 kb on - strand at 1625.278 kb on + strand at 1625.278 kb on + strand at 1625.286 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with D-Glucose and Ammonium Sulfate remove 1,622,883 + GFF1594 0.76 -1.4 1,622,883 + GFF1594 0.76 -1.8 1,622,891 - GFF1594 0.77 -1.2 1,622,891 - GFF1594 0.77 -1.2 1,622,922 + GFF1594 0.80 -1.6 1,622,930 - GFF1594 0.81 -3.0 1,622,933 + GFF1594 0.81 -1.5 1,622,949 + GFF1594 0.83 -1.2 1,622,983 + GFF1594 0.86 -1.5 1,623,009 - GFF1594 0.89 -2.7 1,623,087 - -1.6 1,623,087 - -1.8 1,623,089 + -1.4 1,623,118 + -2.5 1,623,213 + GFF1595 0.16 -1.8 1,623,745 + -0.3 1,623,751 + -1.6 1,623,753 - -0.1 1,623,765 - -0.9 1,623,816 + -2.0 1,623,823 + -0.5 1,623,824 - +0.4 1,623,837 + +0.3 1,623,858 + GFF1596 0.13 +1.7 1,623,858 + GFF1596 0.13 +0.4 1,623,858 + GFF1596 0.13 -0.7 1,623,858 + GFF1596 0.13 -0.1 1,623,858 + GFF1596 0.13 +1.3 1,623,858 + GFF1596 0.13 +0.7 1,623,858 + GFF1596 0.13 -0.1 1,623,864 + GFF1596 0.14 +0.4 1,623,866 - GFF1596 0.15 -0.4 1,623,866 - GFF1596 0.15 +0.2 1,623,886 + GFF1596 0.19 -0.4 1,623,891 + GFF1596 0.20 -0.7 1,623,892 - GFF1596 0.20 -0.2 1,623,960 + GFF1596 0.33 -0.0 1,623,960 + GFF1596 0.33 +0.3 1,623,960 + GFF1596 0.33 -0.7 1,623,960 + GFF1596 0.33 -0.6 1,623,965 + GFF1596 0.34 -0.2 1,623,968 - GFF1596 0.34 +1.5 1,623,982 + GFF1596 0.37 -2.1 1,623,987 + GFF1596 0.38 -1.0 1,623,987 + GFF1596 0.38 -2.3 1,624,016 + GFF1596 0.43 -0.5 1,624,016 + GFF1596 0.43 -0.1 1,624,017 + GFF1596 0.44 +0.1 1,624,017 + GFF1596 0.44 -0.1 1,624,017 + GFF1596 0.44 -0.0 1,624,017 + GFF1596 0.44 -2.3 1,624,017 + GFF1596 0.44 +0.7 1,624,017 + GFF1596 0.44 -1.3 1,624,017 + GFF1596 0.44 +0.7 1,624,017 + GFF1596 0.44 +0.9 1,624,017 + GFF1596 0.44 -1.4 1,624,017 + GFF1596 0.44 -1.7 1,624,017 + GFF1596 0.44 -0.8 1,624,017 + GFF1596 0.44 -0.6 1,624,017 + GFF1596 0.44 +0.3 1,624,023 - GFF1596 0.45 -0.3 1,624,024 - GFF1596 0.45 +0.3 1,624,024 - GFF1596 0.45 -0.2 1,624,024 - GFF1596 0.45 +0.0 1,624,025 - GFF1596 0.45 -0.7 1,624,025 - GFF1596 0.45 +0.5 1,624,025 - GFF1596 0.45 -1.2 1,624,025 - GFF1596 0.45 -1.0 1,624,025 - GFF1596 0.45 -0.7 1,624,025 - GFF1596 0.45 +0.9 1,624,025 - GFF1596 0.45 +0.5 1,624,025 - GFF1596 0.45 -0.5 1,624,025 - GFF1596 0.45 +1.4 1,624,025 - GFF1596 0.45 -0.9 1,624,025 - GFF1596 0.45 +0.9 1,624,025 - GFF1596 0.45 +1.2 1,624,025 - GFF1596 0.45 -1.3 1,624,025 - GFF1596 0.45 -1.7 1,624,046 - GFF1596 0.49 +0.1 1,624,065 + GFF1596 0.53 -0.2 1,624,070 + GFF1596 0.54 -0.7 1,624,140 + GFF1596 0.67 +0.9 1,624,151 + GFF1596 0.69 -0.6 1,624,183 - GFF1596 0.75 -0.6 1,624,200 + GFF1596 0.79 -0.7 1,624,213 - GFF1596 0.81 -2.1 1,624,247 + GFF1596 0.88 -0.8 1,624,255 - GFF1596 0.89 +0.6 1,624,298 + -1.7 1,624,299 + -1.0 1,624,299 + -0.6 1,624,299 + +0.8 1,624,299 + +1.8 1,624,299 + -1.0 1,624,299 + -1.5 1,624,299 + -0.8 1,624,304 + -0.5 1,624,304 + +0.4 1,624,304 + -0.3 1,624,304 + +0.6 1,624,307 - +0.8 1,624,307 - +0.9 1,624,307 - +0.2 1,624,341 + -0.0 1,624,378 + -0.6 1,624,386 - GFF1597 0.11 -0.3 1,624,428 - GFF1597 0.21 +0.0 1,624,438 + GFF1597 0.24 +0.3 1,624,438 + GFF1597 0.24 -0.4 1,624,438 + GFF1597 0.24 -0.3 1,624,446 - GFF1597 0.26 -1.8 1,624,446 - GFF1597 0.26 -0.1 1,624,446 - GFF1597 0.26 -0.1 1,624,446 - GFF1597 0.26 +0.5 1,624,448 + GFF1597 0.26 +0.4 1,624,451 - GFF1597 0.27 +0.4 1,624,451 - GFF1597 0.27 -0.2 1,624,456 - GFF1597 0.28 +1.7 1,624,456 - GFF1597 0.28 -0.4 1,624,468 + GFF1597 0.31 -0.2 1,624,468 + GFF1597 0.31 -2.8 1,624,473 + GFF1597 0.32 -0.6 1,624,473 + GFF1597 0.32 -1.4 1,624,476 - GFF1597 0.33 +0.9 1,624,476 - GFF1597 0.33 +0.0 1,624,481 - GFF1597 0.34 +0.6 1,624,516 + GFF1597 0.42 -0.5 1,624,521 + GFF1597 0.44 -0.0 1,624,532 + GFF1597 0.46 -1.4 1,624,534 + GFF1597 0.47 -0.8 1,624,539 + GFF1597 0.48 +1.4 1,624,540 - GFF1597 0.48 -0.4 1,624,542 - GFF1597 0.49 +0.3 1,624,545 - GFF1597 0.49 +2.4 1,624,547 + GFF1597 0.50 -0.2 1,624,561 - GFF1597 0.53 -1.5 1,624,572 + GFF1597 0.56 -2.5 1,624,580 - GFF1597 0.58 +0.3 1,624,580 - GFF1597 0.58 -0.5 1,624,580 - GFF1597 0.58 +1.4 1,624,588 - GFF1597 0.60 -0.6 1,624,588 - GFF1597 0.60 +0.4 1,624,597 + GFF1597 0.62 -0.4 1,624,600 - GFF1597 0.62 +0.7 1,624,604 + GFF1597 0.63 -0.5 1,624,610 - GFF1597 0.65 +0.1 1,624,619 + GFF1597 0.67 -0.7 1,624,630 + GFF1597 0.70 +0.0 1,624,630 + GFF1597 0.70 +0.1 1,624,630 + GFF1597 0.70 +0.0 1,624,630 + GFF1597 0.70 -0.3 1,624,630 + GFF1597 0.70 +0.7 1,624,635 + GFF1597 0.71 -0.5 1,624,638 - GFF1597 0.71 -0.6 1,624,638 - GFF1597 0.71 -0.4 1,624,643 + GFF1597 0.73 +0.0 1,624,643 + GFF1597 0.73 +0.5 1,624,651 - GFF1597 0.75 -0.4 1,624,683 - GFF1597 0.82 +0.3 1,624,688 - GFF1597 0.83 -1.5 1,624,719 + -1.1 1,624,727 - -0.1 1,624,727 - +0.2 1,624,727 - -2.1 1,624,741 - -1.2 1,624,745 + -1.8 1,624,747 - -0.6 1,624,752 + -1.0 1,624,752 + -1.3 1,624,775 + -0.5 1,624,778 - +0.3 1,624,778 - +0.9 1,624,816 - GFF1598 0.12 -0.6 1,624,856 + GFF1598 0.20 +0.2 1,624,858 - GFF1598 0.20 -2.6 1,624,893 + GFF1598 0.27 -0.5 1,624,893 + GFF1598 0.27 -0.1 1,624,898 + GFF1598 0.28 +0.3 1,624,898 + GFF1598 0.28 -0.5 1,624,906 - GFF1598 0.29 -1.1 1,624,906 - GFF1598 0.29 -1.6 1,624,911 - GFF1598 0.30 -0.5 1,624,929 - GFF1598 0.34 +0.9 1,624,929 - GFF1598 0.34 +0.4 1,624,929 - GFF1598 0.34 +0.8 1,624,929 - GFF1598 0.34 +0.2 1,624,929 - GFF1598 0.34 -0.8 1,624,936 - GFF1598 0.35 -0.2 1,624,945 + GFF1598 0.37 +0.8 1,624,947 - GFF1598 0.37 -0.4 1,624,965 - GFF1598 0.41 -1.4 1,624,965 - GFF1598 0.41 -0.1 1,624,982 + GFF1598 0.44 -0.9 1,624,994 - GFF1598 0.47 +0.8 1,625,010 - GFF1598 0.50 -0.5 1,625,026 - GFF1598 0.53 -0.5 1,625,040 - GFF1598 0.55 -0.1 1,625,042 + GFF1598 0.56 -0.9 1,625,042 + GFF1598 0.56 -0.1 1,625,066 - GFF1598 0.60 +1.3 1,625,072 - GFF1598 0.62 -0.2 1,625,088 + GFF1598 0.65 -0.0 1,625,090 + GFF1598 0.65 -0.5 1,625,090 + GFF1598 0.65 +0.1 1,625,104 + GFF1598 0.68 +0.1 1,625,112 + GFF1598 0.69 -0.1 1,625,112 - GFF1598 0.69 -0.8 1,625,112 - GFF1598 0.69 +0.0 1,625,115 - GFF1598 0.70 +0.1 1,625,141 - GFF1598 0.75 -2.0 1,625,145 + GFF1598 0.76 +0.5 1,625,153 - GFF1598 0.77 -2.0 1,625,155 + GFF1598 0.78 -0.1 1,625,176 + GFF1598 0.82 -0.3 1,625,178 - GFF1598 0.82 +0.4 1,625,184 + GFF1598 0.83 -0.1 1,625,189 + GFF1598 0.84 +0.5 1,625,192 - GFF1598 0.85 +0.7 1,625,192 - GFF1598 0.85 -1.1 1,625,192 - GFF1598 0.85 -1.3 1,625,192 - GFF1598 0.85 -0.7 1,625,194 + GFF1598 0.85 +0.9 1,625,194 + GFF1598 0.85 +0.4 1,625,200 + GFF1598 0.86 -0.8 1,625,202 - GFF1598 0.87 +0.5 1,625,202 - GFF1598 0.87 -2.0 1,625,228 + -0.9 1,625,233 + -1.9 1,625,233 + -1.6 1,625,236 - +0.5 1,625,241 - -1.0 1,625,241 - -0.3 1,625,241 - -1.3 1,625,241 - -1.0 1,625,246 - +0.2 1,625,272 - -1.4 1,625,278 + -0.2 1,625,278 + -0.6 1,625,286 - -0.8
Or see this region's nucleotide sequence