Experiment: EMM_noCarbon with D-Glucose and Ammonium Sulfate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF132 and GFF133 are separated by 0 nucleotides GFF133 and GFF134 overlap by 4 nucleotides GFF134 and GFF135 are separated by 226 nucleotides
GFF132 - Methylmalonyl-CoA mutase (EC 5.4.99.2) / B12 binding domain of Methylmalonyl-CoA mutase (EC 5.4.99.2), at 125,798 to 127,951
GFF132
GFF133 - Biotin synthase (EC 2.8.1.6), at 127,952 to 128,965
GFF133
GFF134 - hypothetical protein, at 128,962 to 129,078
GFF134
GFF135 - Propionyl-CoA carboxylase biotin-containing subunit (EC 6.4.1.3), at 129,305 to 131,305
GFF135
Position (kb) 127
128
129 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 126.994 kb on - strand, within GFF132 at 127.048 kb on + strand, within GFF132 at 127.048 kb on + strand, within GFF132 at 127.056 kb on - strand, within GFF132 at 127.071 kb on + strand, within GFF132 at 127.079 kb on - strand, within GFF132 at 127.196 kb on + strand, within GFF132 at 127.197 kb on - strand, within GFF132 at 127.278 kb on - strand, within GFF132 at 127.468 kb on - strand, within GFF132 at 127.469 kb on + strand, within GFF132 at 127.852 kb on - strand at 127.915 kb on + strand at 127.915 kb on + strand at 127.919 kb on - strand at 127.923 kb on - strand at 127.923 kb on - strand at 127.931 kb on + strand at 127.931 kb on + strand at 127.932 kb on + strand at 127.936 kb on + strand at 127.936 kb on + strand at 127.939 kb on - strand at 127.944 kb on - strand at 127.944 kb on - strand at 127.946 kb on + strand at 127.946 kb on + strand at 127.960 kb on + strand at 127.968 kb on - strand at 127.984 kb on + strand at 127.985 kb on + strand at 128.012 kb on - strand at 128.062 kb on - strand, within GFF133 at 128.092 kb on + strand, within GFF133 at 128.092 kb on + strand, within GFF133 at 128.100 kb on + strand, within GFF133 at 128.100 kb on - strand, within GFF133 at 128.100 kb on - strand, within GFF133 at 128.103 kb on - strand, within GFF133 at 128.127 kb on + strand, within GFF133 at 128.134 kb on + strand, within GFF133 at 128.134 kb on + strand, within GFF133 at 128.134 kb on + strand, within GFF133 at 128.135 kb on - strand, within GFF133 at 128.135 kb on - strand, within GFF133 at 128.135 kb on - strand, within GFF133 at 128.135 kb on - strand, within GFF133 at 128.148 kb on + strand, within GFF133 at 128.161 kb on - strand, within GFF133 at 128.184 kb on - strand, within GFF133 at 128.191 kb on + strand, within GFF133 at 128.207 kb on - strand, within GFF133 at 128.212 kb on + strand, within GFF133 at 128.218 kb on - strand, within GFF133 at 128.218 kb on - strand, within GFF133 at 128.230 kb on + strand, within GFF133 at 128.230 kb on + strand, within GFF133 at 128.230 kb on + strand, within GFF133 at 128.230 kb on + strand, within GFF133 at 128.237 kb on + strand, within GFF133 at 128.239 kb on - strand, within GFF133 at 128.247 kb on + strand, within GFF133 at 128.278 kb on - strand, within GFF133 at 128.278 kb on - strand, within GFF133 at 128.280 kb on + strand, within GFF133 at 128.280 kb on + strand, within GFF133 at 128.280 kb on + strand, within GFF133 at 128.288 kb on - strand, within GFF133 at 128.293 kb on + strand, within GFF133 at 128.317 kb on + strand, within GFF133 at 128.317 kb on + strand, within GFF133 at 128.317 kb on + strand, within GFF133 at 128.317 kb on + strand, within GFF133 at 128.317 kb on + strand, within GFF133 at 128.317 kb on + strand, within GFF133 at 128.317 kb on + strand, within GFF133 at 128.317 kb on + strand, within GFF133 at 128.323 kb on - strand, within GFF133 at 128.325 kb on + strand, within GFF133 at 128.325 kb on - strand, within GFF133 at 128.325 kb on - strand, within GFF133 at 128.325 kb on - strand, within GFF133 at 128.325 kb on - strand, within GFF133 at 128.325 kb on - strand, within GFF133 at 128.325 kb on - strand, within GFF133 at 128.325 kb on - strand, within GFF133 at 128.325 kb on - strand, within GFF133 at 128.358 kb on + strand, within GFF133 at 128.358 kb on + strand, within GFF133 at 128.371 kb on - strand, within GFF133 at 128.373 kb on + strand, within GFF133 at 128.381 kb on - strand, within GFF133 at 128.406 kb on + strand, within GFF133 at 128.422 kb on + strand, within GFF133 at 128.430 kb on - strand, within GFF133 at 128.439 kb on + strand, within GFF133 at 128.442 kb on - strand, within GFF133 at 128.456 kb on + strand, within GFF133 at 128.466 kb on - strand, within GFF133 at 128.476 kb on - strand, within GFF133 at 128.486 kb on + strand, within GFF133 at 128.494 kb on - strand, within GFF133 at 128.549 kb on - strand, within GFF133 at 128.555 kb on + strand, within GFF133 at 128.563 kb on - strand, within GFF133 at 128.572 kb on - strand, within GFF133 at 128.582 kb on - strand, within GFF133 at 128.584 kb on + strand, within GFF133 at 128.596 kb on + strand, within GFF133 at 128.600 kb on + strand, within GFF133 at 128.612 kb on + strand, within GFF133 at 128.615 kb on + strand, within GFF133 at 128.620 kb on - strand, within GFF133 at 128.620 kb on - strand, within GFF133 at 128.620 kb on - strand, within GFF133 at 128.632 kb on - strand, within GFF133 at 128.655 kb on + strand, within GFF133 at 128.660 kb on + strand, within GFF133 at 128.660 kb on + strand, within GFF133 at 128.660 kb on + strand, within GFF133 at 128.660 kb on + strand, within GFF133 at 128.660 kb on + strand, within GFF133 at 128.668 kb on - strand, within GFF133 at 128.668 kb on - strand, within GFF133 at 128.668 kb on - strand, within GFF133 at 128.668 kb on - strand, within GFF133 at 128.668 kb on - strand, within GFF133 at 128.668 kb on - strand, within GFF133 at 128.668 kb on - strand, within GFF133 at 128.668 kb on - strand, within GFF133 at 128.668 kb on - strand, within GFF133 at 128.668 kb on - strand, within GFF133 at 128.668 kb on - strand, within GFF133 at 128.668 kb on - strand, within GFF133 at 128.672 kb on + strand, within GFF133 at 128.711 kb on + strand, within GFF133 at 128.724 kb on + strand, within GFF133 at 128.724 kb on + strand, within GFF133 at 128.742 kb on - strand, within GFF133 at 128.757 kb on + strand, within GFF133 at 128.762 kb on + strand, within GFF133 at 128.765 kb on - strand, within GFF133 at 128.767 kb on + strand, within GFF133 at 128.767 kb on + strand, within GFF133 at 128.767 kb on + strand, within GFF133 at 128.770 kb on - strand, within GFF133 at 128.799 kb on + strand, within GFF133 at 128.855 kb on + strand, within GFF133 at 128.855 kb on + strand, within GFF133 at 128.855 kb on + strand, within GFF133 at 128.863 kb on - strand, within GFF133 at 128.863 kb on - strand, within GFF133 at 128.863 kb on - strand, within GFF133 at 128.872 kb on + strand at 128.873 kb on + strand at 128.908 kb on + strand at 128.908 kb on + strand at 128.916 kb on - strand at 128.960 kb on + strand at 128.970 kb on + strand at 128.973 kb on + strand at 128.996 kb on - strand, within GFF134 at 129.008 kb on + strand, within GFF134 at 129.017 kb on - strand, within GFF134 at 129.030 kb on + strand, within GFF134 at 129.038 kb on - strand, within GFF134 at 129.038 kb on - strand, within GFF134 at 129.046 kb on - strand, within GFF134 at 129.050 kb on - strand, within GFF134 at 129.061 kb on + strand, within GFF134 at 129.095 kb on + strand at 129.120 kb on + strand at 129.120 kb on + strand at 129.120 kb on + strand at 129.120 kb on + strand at 129.120 kb on + strand at 129.120 kb on + strand at 129.120 kb on + strand at 129.128 kb on - strand at 129.128 kb on - strand at 129.128 kb on - strand at 129.130 kb on + strand at 129.130 kb on + strand at 129.132 kb on + strand at 129.137 kb on + strand at 129.147 kb on + strand at 129.157 kb on + strand at 129.164 kb on + strand at 129.171 kb on + strand at 129.171 kb on + strand at 129.171 kb on + strand at 129.172 kb on - strand at 129.176 kb on + strand at 129.179 kb on - strand at 129.179 kb on - strand at 129.181 kb on + strand at 129.181 kb on + strand at 129.252 kb on - strand at 129.274 kb on - strand at 129.344 kb on + strand at 129.356 kb on + strand at 129.366 kb on + strand at 129.366 kb on + strand at 129.368 kb on + strand at 129.368 kb on + strand at 129.376 kb on - strand at 129.376 kb on - strand at 129.390 kb on - strand at 129.390 kb on - strand at 129.390 kb on - strand at 129.390 kb on - strand at 129.398 kb on + strand at 129.400 kb on - strand at 129.402 kb on + strand at 129.442 kb on + strand at 129.451 kb on + strand at 129.476 kb on + strand at 129.476 kb on + strand at 129.476 kb on + strand at 129.481 kb on + strand at 129.481 kb on + strand at 129.481 kb on + strand at 129.481 kb on + strand at 129.484 kb on - strand at 129.484 kb on - strand at 129.484 kb on - strand at 129.489 kb on - strand at 129.489 kb on - strand at 129.489 kb on - strand at 129.510 kb on - strand, within GFF135 at 129.539 kb on + strand, within GFF135 at 129.633 kb on - strand, within GFF135 at 129.681 kb on - strand, within GFF135 at 129.696 kb on - strand, within GFF135 at 129.696 kb on - strand, within GFF135 at 129.701 kb on - strand, within GFF135 at 129.708 kb on + strand, within GFF135 at 129.732 kb on + strand, within GFF135 at 129.740 kb on + strand, within GFF135 at 129.763 kb on - strand, within GFF135 at 129.773 kb on - strand, within GFF135 at 129.774 kb on + strand, within GFF135 at 129.781 kb on + strand, within GFF135 at 129.781 kb on + strand, within GFF135 at 129.782 kb on + strand, within GFF135 at 129.783 kb on - strand, within GFF135 at 129.783 kb on - strand, within GFF135 at 129.783 kb on - strand, within GFF135 at 129.783 kb on - strand, within GFF135 at 129.789 kb on - strand, within GFF135 at 129.806 kb on + strand, within GFF135 at 129.811 kb on + strand, within GFF135 at 129.817 kb on + strand, within GFF135 at 129.826 kb on - strand, within GFF135 at 129.846 kb on + strand, within GFF135 at 129.846 kb on + strand, within GFF135 at 129.846 kb on + strand, within GFF135 at 129.847 kb on - strand, within GFF135 at 129.854 kb on - strand, within GFF135 at 129.854 kb on - strand, within GFF135 at 129.854 kb on - strand, within GFF135 at 129.861 kb on + strand, within GFF135 at 129.864 kb on - strand, within GFF135 at 129.869 kb on + strand, within GFF135 at 129.869 kb on + strand, within GFF135 at 129.869 kb on + strand, within GFF135 at 129.869 kb on - strand, within GFF135 at 129.870 kb on - strand, within GFF135 at 129.877 kb on - strand, within GFF135 at 129.877 kb on - strand, within GFF135 at 129.877 kb on - strand, within GFF135 at 129.961 kb on + strand, within GFF135 at 129.961 kb on + strand, within GFF135
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with D-Glucose and Ammonium Sulfate remove 126,994 - GFF132 0.56 -0.1 127,048 + GFF132 0.58 -1.4 127,048 + GFF132 0.58 -1.4 127,056 - GFF132 0.58 -0.2 127,071 + GFF132 0.59 -1.6 127,079 - GFF132 0.59 -0.6 127,196 + GFF132 0.65 -0.7 127,197 - GFF132 0.65 -1.4 127,278 - GFF132 0.69 -1.4 127,468 - GFF132 0.78 -0.7 127,469 + GFF132 0.78 -2.2 127,852 - -0.2 127,915 + -0.4 127,915 + +1.4 127,919 - +0.4 127,923 - +0.7 127,923 - -0.7 127,931 + -1.1 127,931 + -1.2 127,932 + -1.9 127,936 + -1.0 127,936 + -0.2 127,939 - -1.9 127,944 - -0.2 127,944 - -0.5 127,946 + +0.1 127,946 + -3.8 127,960 + -0.1 127,968 - -1.5 127,984 + -1.4 127,985 + +0.2 128,012 - -0.5 128,062 - GFF133 0.11 -2.7 128,092 + GFF133 0.14 -1.2 128,092 + GFF133 0.14 -2.4 128,100 + GFF133 0.15 +0.8 128,100 - GFF133 0.15 +0.3 128,100 - GFF133 0.15 -0.2 128,103 - GFF133 0.15 +0.3 128,127 + GFF133 0.17 +0.7 128,134 + GFF133 0.18 -0.2 128,134 + GFF133 0.18 -0.2 128,134 + GFF133 0.18 -1.3 128,135 - GFF133 0.18 -0.8 128,135 - GFF133 0.18 -1.4 128,135 - GFF133 0.18 +0.3 128,135 - GFF133 0.18 -0.1 128,148 + GFF133 0.19 -0.5 128,161 - GFF133 0.21 -0.1 128,184 - GFF133 0.23 -1.9 128,191 + GFF133 0.24 +0.4 128,207 - GFF133 0.25 -1.1 128,212 + GFF133 0.26 -0.9 128,218 - GFF133 0.26 -0.4 128,218 - GFF133 0.26 -0.0 128,230 + GFF133 0.27 -1.8 128,230 + GFF133 0.27 +0.6 128,230 + GFF133 0.27 -0.1 128,230 + GFF133 0.27 +0.5 128,237 + GFF133 0.28 -0.6 128,239 - GFF133 0.28 -0.7 128,247 + GFF133 0.29 +0.5 128,278 - GFF133 0.32 -1.1 128,278 - GFF133 0.32 -0.9 128,280 + GFF133 0.32 +0.4 128,280 + GFF133 0.32 -1.3 128,280 + GFF133 0.32 -0.5 128,288 - GFF133 0.33 +0.3 128,293 + GFF133 0.34 +0.0 128,317 + GFF133 0.36 +1.3 128,317 + GFF133 0.36 -2.2 128,317 + GFF133 0.36 +0.8 128,317 + GFF133 0.36 +0.4 128,317 + GFF133 0.36 +1.1 128,317 + GFF133 0.36 +0.5 128,317 + GFF133 0.36 -0.8 128,317 + GFF133 0.36 -0.6 128,323 - GFF133 0.37 -0.2 128,325 + GFF133 0.37 +0.8 128,325 - GFF133 0.37 -1.7 128,325 - GFF133 0.37 -1.2 128,325 - GFF133 0.37 +0.3 128,325 - GFF133 0.37 -2.0 128,325 - GFF133 0.37 -2.1 128,325 - GFF133 0.37 -1.2 128,325 - GFF133 0.37 -1.7 128,325 - GFF133 0.37 +0.4 128,358 + GFF133 0.40 -0.1 128,358 + GFF133 0.40 -1.2 128,371 - GFF133 0.41 -1.0 128,373 + GFF133 0.42 +0.9 128,381 - GFF133 0.42 -1.1 128,406 + GFF133 0.45 -0.5 128,422 + GFF133 0.46 +1.0 128,430 - GFF133 0.47 +1.2 128,439 + GFF133 0.48 +0.7 128,442 - GFF133 0.48 -0.7 128,456 + GFF133 0.50 -1.0 128,466 - GFF133 0.51 +0.1 128,476 - GFF133 0.52 -0.5 128,486 + GFF133 0.53 +0.3 128,494 - GFF133 0.53 +0.2 128,549 - GFF133 0.59 -1.1 128,555 + GFF133 0.59 -0.3 128,563 - GFF133 0.60 +0.1 128,572 - GFF133 0.61 +0.5 128,582 - GFF133 0.62 -0.4 128,584 + GFF133 0.62 -0.5 128,596 + GFF133 0.64 +0.6 128,600 + GFF133 0.64 -1.1 128,612 + GFF133 0.65 +0.6 128,615 + GFF133 0.65 -0.2 128,620 - GFF133 0.66 -0.1 128,620 - GFF133 0.66 -0.2 128,620 - GFF133 0.66 -0.0 128,632 - GFF133 0.67 -1.4 128,655 + GFF133 0.69 +0.2 128,660 + GFF133 0.70 -1.6 128,660 + GFF133 0.70 +0.3 128,660 + GFF133 0.70 -0.4 128,660 + GFF133 0.70 -2.0 128,660 + GFF133 0.70 +0.3 128,668 - GFF133 0.71 -1.5 128,668 - GFF133 0.71 +1.0 128,668 - GFF133 0.71 -0.3 128,668 - GFF133 0.71 +0.8 128,668 - GFF133 0.71 +0.1 128,668 - GFF133 0.71 +0.0 128,668 - GFF133 0.71 -0.1 128,668 - GFF133 0.71 +0.1 128,668 - GFF133 0.71 +0.1 128,668 - GFF133 0.71 -0.6 128,668 - GFF133 0.71 -0.4 128,668 - GFF133 0.71 +0.2 128,672 + GFF133 0.71 +0.1 128,711 + GFF133 0.75 -0.5 128,724 + GFF133 0.76 -0.2 128,724 + GFF133 0.76 +1.0 128,742 - GFF133 0.78 -0.8 128,757 + GFF133 0.79 -0.1 128,762 + GFF133 0.80 -0.2 128,765 - GFF133 0.80 -0.5 128,767 + GFF133 0.80 +0.5 128,767 + GFF133 0.80 -1.2 128,767 + GFF133 0.80 +0.8 128,770 - GFF133 0.81 +1.2 128,799 + GFF133 0.84 -0.4 128,855 + GFF133 0.89 -0.2 128,855 + GFF133 0.89 -2.0 128,855 + GFF133 0.89 +0.9 128,863 - GFF133 0.90 -0.1 128,863 - GFF133 0.90 +0.3 128,863 - GFF133 0.90 -0.8 128,872 + -0.1 128,873 + -0.3 128,908 + +0.1 128,908 + -1.6 128,916 - -0.7 128,960 + +1.1 128,970 + +0.3 128,973 + -2.1 128,996 - GFF134 0.29 -0.5 129,008 + GFF134 0.39 -0.3 129,017 - GFF134 0.47 -0.7 129,030 + GFF134 0.58 +0.1 129,038 - GFF134 0.65 -0.6 129,038 - GFF134 0.65 +0.3 129,046 - GFF134 0.72 +0.2 129,050 - GFF134 0.75 +0.6 129,061 + GFF134 0.85 -1.4 129,095 + +0.3 129,120 + -2.0 129,120 + -1.1 129,120 + -0.9 129,120 + -0.3 129,120 + -1.0 129,120 + +0.6 129,120 + -0.1 129,128 - +0.3 129,128 - -1.5 129,128 - -1.2 129,130 + +1.6 129,130 + +0.9 129,132 + +0.2 129,137 + -0.3 129,147 + -1.2 129,157 + -0.7 129,164 + +0.1 129,171 + -2.0 129,171 + +0.6 129,171 + -1.7 129,172 - +0.3 129,176 + +1.4 129,179 - -0.5 129,179 - -2.3 129,181 + +1.0 129,181 + +0.5 129,252 - +1.3 129,274 - +0.1 129,344 + +0.7 129,356 + -0.4 129,366 + -0.4 129,366 + -3.0 129,368 + +1.0 129,368 + -0.6 129,376 - +1.6 129,376 - +0.3 129,390 - -2.3 129,390 - -0.1 129,390 - -1.2 129,390 - +0.1 129,398 + -1.1 129,400 - -0.4 129,402 + -0.1 129,442 + +1.1 129,451 + +0.4 129,476 + -0.7 129,476 + +0.6 129,476 + +0.2 129,481 + +0.7 129,481 + -0.4 129,481 + -0.7 129,481 + -1.7 129,484 - -0.1 129,484 - -2.6 129,484 - +0.1 129,489 - -1.2 129,489 - -0.3 129,489 - -0.2 129,510 - GFF135 0.10 +1.1 129,539 + GFF135 0.12 +0.6 129,633 - GFF135 0.16 +0.2 129,681 - GFF135 0.19 -0.5 129,696 - GFF135 0.20 -2.0 129,696 - GFF135 0.20 +1.0 129,701 - GFF135 0.20 -1.3 129,708 + GFF135 0.20 -0.6 129,732 + GFF135 0.21 -0.6 129,740 + GFF135 0.22 -1.4 129,763 - GFF135 0.23 +0.2 129,773 - GFF135 0.23 -0.5 129,774 + GFF135 0.23 +0.0 129,781 + GFF135 0.24 +0.1 129,781 + GFF135 0.24 -0.3 129,782 + GFF135 0.24 +0.4 129,783 - GFF135 0.24 -0.7 129,783 - GFF135 0.24 -0.9 129,783 - GFF135 0.24 -2.2 129,783 - GFF135 0.24 -0.8 129,789 - GFF135 0.24 +0.5 129,806 + GFF135 0.25 -1.6 129,811 + GFF135 0.25 -1.7 129,817 + GFF135 0.26 +0.9 129,826 - GFF135 0.26 +0.3 129,846 + GFF135 0.27 -0.4 129,846 + GFF135 0.27 -0.4 129,846 + GFF135 0.27 -0.2 129,847 - GFF135 0.27 -2.7 129,854 - GFF135 0.27 -1.1 129,854 - GFF135 0.27 -1.8 129,854 - GFF135 0.27 -1.6 129,861 + GFF135 0.28 -1.9 129,864 - GFF135 0.28 -0.0 129,869 + GFF135 0.28 -0.3 129,869 + GFF135 0.28 +0.7 129,869 + GFF135 0.28 +0.2 129,869 - GFF135 0.28 +0.3 129,870 - GFF135 0.28 -0.1 129,877 - GFF135 0.29 -1.3 129,877 - GFF135 0.29 +0.7 129,877 - GFF135 0.29 +0.6 129,961 + GFF135 0.33 -1.6 129,961 + GFF135 0.33 +0.2
Or see this region's nucleotide sequence