Experiment: EMM_noCarbon with D-Glucose and Ammonium Sulfate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF129 and GFF130 are separated by 94 nucleotides GFF130 and GFF131 are separated by 49 nucleotides GFF131 and GFF132 are separated by 167 nucleotides
GFF129 - Propionyl-CoA carboxylase carboxyl transferase subunit (EC 6.4.1.3), at 123,223 to 124,755
GFF129
GFF130 - Excinuclease ABC, C subunit-like, at 124,850 to 125,140
GFF130
GFF131 - Methylmalonyl-CoA epimerase (EC 5.1.99.1) @ Ethylmalonyl-CoA epimerase, at 125,190 to 125,630
GFF131
GFF132 - Methylmalonyl-CoA mutase (EC 5.4.99.2) / B12 binding domain of Methylmalonyl-CoA mutase (EC 5.4.99.2), at 125,798 to 127,951
GFF132
Position (kb)
125
126 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 124.229 kb on + strand, within GFF129 at 124.246 kb on - strand, within GFF129 at 124.266 kb on + strand, within GFF129 at 124.284 kb on - strand, within GFF129 at 124.323 kb on + strand, within GFF129 at 124.323 kb on + strand, within GFF129 at 124.323 kb on + strand, within GFF129 at 124.360 kb on - strand, within GFF129 at 124.429 kb on + strand, within GFF129 at 124.477 kb on + strand, within GFF129 at 124.485 kb on - strand, within GFF129 at 124.641 kb on - strand at 124.646 kb on + strand at 124.682 kb on + strand at 124.690 kb on - strand at 124.690 kb on - strand at 124.690 kb on - strand at 124.690 kb on - strand at 124.690 kb on - strand at 124.690 kb on - strand at 124.692 kb on + strand at 124.692 kb on + strand at 124.712 kb on - strand at 124.714 kb on + strand at 124.724 kb on + strand at 124.779 kb on - strand at 124.779 kb on - strand at 124.825 kb on + strand at 124.830 kb on + strand at 124.830 kb on + strand at 124.840 kb on + strand at 124.862 kb on + strand at 124.862 kb on + strand at 124.862 kb on + strand at 124.862 kb on + strand at 124.862 kb on + strand at 124.894 kb on + strand, within GFF130 at 124.895 kb on + strand, within GFF130 at 124.895 kb on + strand, within GFF130 at 124.895 kb on + strand, within GFF130 at 124.897 kb on + strand, within GFF130 at 124.899 kb on + strand, within GFF130 at 124.899 kb on + strand, within GFF130 at 124.902 kb on - strand, within GFF130 at 124.902 kb on - strand, within GFF130 at 124.905 kb on - strand, within GFF130 at 124.910 kb on + strand, within GFF130 at 124.911 kb on + strand, within GFF130 at 124.911 kb on + strand, within GFF130 at 124.911 kb on + strand, within GFF130 at 124.911 kb on + strand, within GFF130 at 124.912 kb on - strand, within GFF130 at 124.929 kb on + strand, within GFF130 at 124.956 kb on - strand, within GFF130 at 124.956 kb on - strand, within GFF130 at 124.956 kb on - strand, within GFF130 at 124.996 kb on - strand, within GFF130 at 124.996 kb on - strand, within GFF130 at 124.998 kb on + strand, within GFF130 at 125.006 kb on - strand, within GFF130 at 125.016 kb on + strand, within GFF130 at 125.047 kb on + strand, within GFF130 at 125.047 kb on + strand, within GFF130 at 125.047 kb on + strand, within GFF130 at 125.047 kb on + strand, within GFF130 at 125.047 kb on + strand, within GFF130 at 125.050 kb on - strand, within GFF130 at 125.055 kb on - strand, within GFF130 at 125.060 kb on - strand, within GFF130 at 125.092 kb on + strand, within GFF130 at 125.092 kb on + strand, within GFF130 at 125.092 kb on + strand, within GFF130 at 125.100 kb on - strand, within GFF130 at 125.137 kb on - strand at 125.137 kb on - strand at 125.143 kb on - strand at 125.151 kb on + strand at 125.181 kb on + strand at 125.211 kb on - strand at 125.284 kb on + strand, within GFF131 at 125.292 kb on - strand, within GFF131 at 125.329 kb on - strand, within GFF131 at 125.351 kb on - strand, within GFF131 at 125.391 kb on + strand, within GFF131 at 125.391 kb on + strand, within GFF131 at 125.430 kb on - strand, within GFF131 at 125.453 kb on - strand, within GFF131 at 125.455 kb on + strand, within GFF131 at 125.463 kb on - strand, within GFF131 at 125.492 kb on + strand, within GFF131 at 125.575 kb on - strand, within GFF131 at 125.575 kb on - strand, within GFF131 at 125.576 kb on + strand, within GFF131 at 125.584 kb on - strand, within GFF131 at 125.589 kb on - strand at 125.606 kb on - strand at 125.633 kb on - strand at 125.637 kb on - strand at 125.701 kb on + strand at 125.706 kb on + strand at 125.706 kb on + strand at 125.779 kb on + strand at 125.779 kb on + strand at 125.786 kb on + strand at 125.790 kb on + strand at 125.861 kb on + strand at 125.902 kb on + strand at 125.902 kb on + strand at 125.921 kb on + strand at 126.003 kb on - strand at 126.003 kb on - strand at 126.074 kb on + strand, within GFF132 at 126.191 kb on - strand, within GFF132 at 126.338 kb on - strand, within GFF132 at 126.348 kb on + strand, within GFF132 at 126.375 kb on - strand, within GFF132
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with D-Glucose and Ammonium Sulfate remove 124,229 + GFF129 0.66 -0.4 124,246 - GFF129 0.67 -0.1 124,266 + GFF129 0.68 -1.8 124,284 - GFF129 0.69 +0.8 124,323 + GFF129 0.72 -0.6 124,323 + GFF129 0.72 -0.6 124,323 + GFF129 0.72 +0.0 124,360 - GFF129 0.74 -0.6 124,429 + GFF129 0.79 +0.7 124,477 + GFF129 0.82 -0.1 124,485 - GFF129 0.82 +0.5 124,641 - +0.2 124,646 + +0.7 124,682 + +0.3 124,690 - +1.3 124,690 - -1.2 124,690 - +0.4 124,690 - -0.0 124,690 - -1.5 124,690 - -0.3 124,692 + -0.0 124,692 + +0.7 124,712 - -0.4 124,714 + +0.7 124,724 + +0.6 124,779 - +1.4 124,779 - -1.3 124,825 + -0.4 124,830 + +1.2 124,830 + +1.0 124,840 + -0.7 124,862 + -0.3 124,862 + +0.0 124,862 + +0.1 124,862 + +0.3 124,862 + -0.3 124,894 + GFF130 0.15 -0.5 124,895 + GFF130 0.15 +1.0 124,895 + GFF130 0.15 +0.3 124,895 + GFF130 0.15 +1.4 124,897 + GFF130 0.16 +0.3 124,899 + GFF130 0.17 +1.3 124,899 + GFF130 0.17 +1.0 124,902 - GFF130 0.18 -0.6 124,902 - GFF130 0.18 -1.1 124,905 - GFF130 0.19 +0.2 124,910 + GFF130 0.21 -0.2 124,911 + GFF130 0.21 +0.1 124,911 + GFF130 0.21 +0.1 124,911 + GFF130 0.21 -0.0 124,911 + GFF130 0.21 +0.9 124,912 - GFF130 0.21 +1.2 124,929 + GFF130 0.27 -0.3 124,956 - GFF130 0.36 +0.2 124,956 - GFF130 0.36 -0.6 124,956 - GFF130 0.36 -0.5 124,996 - GFF130 0.50 +0.1 124,996 - GFF130 0.50 -2.4 124,998 + GFF130 0.51 -0.6 125,006 - GFF130 0.54 -2.5 125,016 + GFF130 0.57 -0.5 125,047 + GFF130 0.68 +2.0 125,047 + GFF130 0.68 +1.4 125,047 + GFF130 0.68 +0.2 125,047 + GFF130 0.68 +0.2 125,047 + GFF130 0.68 -1.4 125,050 - GFF130 0.69 -1.4 125,055 - GFF130 0.70 +1.1 125,060 - GFF130 0.72 +0.2 125,092 + GFF130 0.83 +0.2 125,092 + GFF130 0.83 -0.5 125,092 + GFF130 0.83 -0.6 125,100 - GFF130 0.86 -0.3 125,137 - -1.8 125,137 - +1.5 125,143 - -0.7 125,151 + +0.8 125,181 + -0.1 125,211 - +0.6 125,284 + GFF131 0.21 +1.8 125,292 - GFF131 0.23 -0.2 125,329 - GFF131 0.32 -0.9 125,351 - GFF131 0.37 -0.4 125,391 + GFF131 0.46 -0.2 125,391 + GFF131 0.46 -0.2 125,430 - GFF131 0.54 +0.1 125,453 - GFF131 0.60 -1.5 125,455 + GFF131 0.60 +0.4 125,463 - GFF131 0.62 -1.3 125,492 + GFF131 0.68 -0.8 125,575 - GFF131 0.87 -1.8 125,575 - GFF131 0.87 -1.0 125,576 + GFF131 0.88 -0.8 125,584 - GFF131 0.89 -0.4 125,589 - -1.2 125,606 - +0.1 125,633 - +1.4 125,637 - -1.2 125,701 + -1.1 125,706 + -2.5 125,706 + -0.0 125,779 + -0.3 125,779 + -0.0 125,786 + +1.7 125,790 + +0.8 125,861 + +0.2 125,902 + +0.4 125,902 + +0.4 125,921 + -0.3 126,003 - +1.6 126,003 - +0.1 126,074 + GFF132 0.13 -1.9 126,191 - GFF132 0.18 +0.8 126,338 - GFF132 0.25 +0.6 126,348 + GFF132 0.26 +1.2 126,375 - GFF132 0.27 +1.1
Or see this region's nucleotide sequence