Experiment: EMM_noCarbon with D-Glucose and Ammonium Sulfate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF817 and GFF818 are separated by 129 nucleotides GFF818 and GFF819 are separated by 142 nucleotides GFF819 and GFF820 are separated by 111 nucleotides
GFF817 - D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95), at 805,822 to 807,402
GFF817
GFF818 - Phosphoserine aminotransferase (EC 2.6.1.52), at 807,532 to 808,695
GFF818
GFF819 - RNA binding S1 domain protein, at 808,838 to 809,554
GFF819
GFF820 - hypothetical protein, at 809,666 to 810,544
GFF820
Position (kb)
807
808
809 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 806.584 kb on - strand, within GFF817 at 806.635 kb on - strand, within GFF817 at 807.010 kb on - strand, within GFF817 at 807.362 kb on + strand at 807.405 kb on + strand at 807.413 kb on - strand at 807.444 kb on + strand at 807.463 kb on + strand at 807.463 kb on + strand at 807.471 kb on - strand at 807.476 kb on + strand at 807.483 kb on + strand at 807.491 kb on - strand at 807.495 kb on + strand at 807.529 kb on + strand at 807.537 kb on - strand at 807.542 kb on + strand at 807.542 kb on + strand at 807.542 kb on + strand at 807.542 kb on + strand at 807.542 kb on + strand at 807.542 kb on + strand at 807.542 kb on + strand at 807.542 kb on + strand at 807.542 kb on + strand at 807.542 kb on + strand at 807.542 kb on + strand at 807.542 kb on + strand at 807.550 kb on - strand at 807.550 kb on - strand at 807.550 kb on - strand at 807.550 kb on - strand at 807.550 kb on - strand at 807.550 kb on - strand at 807.550 kb on - strand at 807.550 kb on - strand at 807.550 kb on - strand at 807.550 kb on - strand at 807.550 kb on - strand at 807.550 kb on - strand at 807.550 kb on - strand at 807.550 kb on - strand at 807.550 kb on - strand at 807.550 kb on - strand at 807.555 kb on - strand at 807.599 kb on + strand at 807.599 kb on + strand at 807.646 kb on + strand at 807.680 kb on + strand, within GFF818 at 807.709 kb on - strand, within GFF818 at 807.712 kb on + strand, within GFF818 at 807.725 kb on + strand, within GFF818 at 807.733 kb on - strand, within GFF818 at 807.734 kb on + strand, within GFF818 at 807.738 kb on + strand, within GFF818 at 807.746 kb on - strand, within GFF818 at 807.746 kb on - strand, within GFF818 at 807.783 kb on + strand, within GFF818 at 807.791 kb on - strand, within GFF818 at 807.809 kb on + strand, within GFF818 at 807.809 kb on + strand, within GFF818 at 807.817 kb on - strand, within GFF818 at 807.817 kb on - strand, within GFF818 at 807.833 kb on + strand, within GFF818 at 807.882 kb on + strand, within GFF818 at 807.949 kb on - strand, within GFF818 at 807.949 kb on - strand, within GFF818 at 807.970 kb on - strand, within GFF818 at 807.976 kb on - strand, within GFF818 at 807.989 kb on + strand, within GFF818 at 807.997 kb on - strand, within GFF818 at 807.997 kb on - strand, within GFF818 at 807.997 kb on - strand, within GFF818 at 807.997 kb on - strand, within GFF818 at 807.997 kb on - strand, within GFF818 at 808.058 kb on - strand, within GFF818 at 808.104 kb on - strand, within GFF818 at 808.109 kb on + strand, within GFF818 at 808.117 kb on - strand, within GFF818 at 808.138 kb on + strand, within GFF818 at 808.146 kb on - strand, within GFF818 at 808.146 kb on - strand, within GFF818 at 808.146 kb on - strand, within GFF818 at 808.195 kb on - strand, within GFF818 at 808.218 kb on + strand, within GFF818 at 808.218 kb on + strand, within GFF818 at 808.226 kb on - strand, within GFF818 at 808.271 kb on - strand, within GFF818 at 808.309 kb on - strand, within GFF818 at 808.311 kb on + strand, within GFF818 at 808.322 kb on + strand, within GFF818 at 808.330 kb on - strand, within GFF818 at 808.330 kb on - strand, within GFF818 at 808.330 kb on - strand, within GFF818 at 808.340 kb on - strand, within GFF818 at 808.340 kb on - strand, within GFF818 at 808.340 kb on - strand, within GFF818 at 808.412 kb on - strand, within GFF818 at 808.469 kb on + strand, within GFF818 at 808.472 kb on - strand, within GFF818 at 808.477 kb on - strand, within GFF818 at 808.477 kb on - strand, within GFF818 at 808.588 kb on + strand at 808.595 kb on + strand at 808.596 kb on - strand at 808.597 kb on - strand at 808.647 kb on - strand at 808.689 kb on - strand at 808.694 kb on - strand at 808.706 kb on + strand at 808.708 kb on + strand at 808.714 kb on - strand at 808.714 kb on - strand at 808.727 kb on + strand at 808.727 kb on + strand at 808.736 kb on - strand at 808.754 kb on + strand at 808.755 kb on + strand at 808.835 kb on + strand at 808.852 kb on + strand at 808.893 kb on + strand at 808.893 kb on + strand at 808.898 kb on + strand at 808.906 kb on - strand at 808.974 kb on + strand, within GFF819 at 808.982 kb on - strand, within GFF819 at 808.992 kb on + strand, within GFF819 at 808.993 kb on - strand, within GFF819 at 808.997 kb on + strand, within GFF819 at 808.997 kb on + strand, within GFF819 at 808.999 kb on + strand, within GFF819 at 809.001 kb on + strand, within GFF819 at 809.001 kb on + strand, within GFF819 at 809.005 kb on - strand, within GFF819 at 809.009 kb on - strand, within GFF819 at 809.009 kb on - strand, within GFF819 at 809.009 kb on - strand, within GFF819 at 809.034 kb on - strand, within GFF819 at 809.036 kb on + strand, within GFF819 at 809.053 kb on + strand, within GFF819 at 809.059 kb on + strand, within GFF819 at 809.059 kb on + strand, within GFF819 at 809.067 kb on - strand, within GFF819 at 809.073 kb on + strand, within GFF819 at 809.105 kb on + strand, within GFF819 at 809.127 kb on + strand, within GFF819 at 809.135 kb on - strand, within GFF819 at 809.135 kb on - strand, within GFF819 at 809.135 kb on - strand, within GFF819 at 809.135 kb on - strand, within GFF819 at 809.142 kb on + strand, within GFF819 at 809.145 kb on - strand, within GFF819 at 809.158 kb on - strand, within GFF819 at 809.165 kb on - strand, within GFF819 at 809.165 kb on - strand, within GFF819 at 809.165 kb on - strand, within GFF819 at 809.170 kb on - strand, within GFF819 at 809.179 kb on - strand, within GFF819 at 809.212 kb on + strand, within GFF819 at 809.217 kb on + strand, within GFF819 at 809.217 kb on + strand, within GFF819 at 809.217 kb on + strand, within GFF819 at 809.217 kb on + strand, within GFF819 at 809.219 kb on + strand, within GFF819 at 809.220 kb on - strand, within GFF819 at 809.225 kb on - strand, within GFF819 at 809.225 kb on - strand, within GFF819 at 809.225 kb on - strand, within GFF819 at 809.225 kb on - strand, within GFF819 at 809.225 kb on - strand, within GFF819 at 809.225 kb on - strand, within GFF819 at 809.227 kb on - strand, within GFF819 at 809.227 kb on - strand, within GFF819 at 809.235 kb on - strand, within GFF819 at 809.239 kb on + strand, within GFF819 at 809.250 kb on - strand, within GFF819 at 809.266 kb on + strand, within GFF819 at 809.266 kb on + strand, within GFF819 at 809.310 kb on + strand, within GFF819 at 809.317 kb on + strand, within GFF819 at 809.317 kb on + strand, within GFF819 at 809.317 kb on + strand, within GFF819 at 809.325 kb on - strand, within GFF819 at 809.330 kb on + strand, within GFF819 at 809.338 kb on - strand, within GFF819 at 809.338 kb on - strand, within GFF819 at 809.359 kb on - strand, within GFF819 at 809.362 kb on + strand, within GFF819 at 809.371 kb on - strand, within GFF819 at 809.383 kb on + strand, within GFF819 at 809.388 kb on + strand, within GFF819 at 809.391 kb on - strand, within GFF819 at 809.398 kb on + strand, within GFF819 at 809.398 kb on + strand, within GFF819 at 809.398 kb on + strand, within GFF819 at 809.398 kb on + strand, within GFF819 at 809.409 kb on - strand, within GFF819 at 809.409 kb on - strand, within GFF819 at 809.441 kb on + strand, within GFF819 at 809.441 kb on + strand, within GFF819 at 809.449 kb on - strand, within GFF819 at 809.449 kb on - strand, within GFF819 at 809.449 kb on - strand, within GFF819 at 809.449 kb on - strand, within GFF819 at 809.449 kb on - strand, within GFF819 at 809.449 kb on - strand, within GFF819 at 809.449 kb on - strand, within GFF819 at 809.449 kb on - strand, within GFF819 at 809.453 kb on - strand, within GFF819 at 809.462 kb on + strand, within GFF819 at 809.462 kb on + strand, within GFF819 at 809.464 kb on + strand, within GFF819 at 809.464 kb on + strand, within GFF819 at 809.464 kb on + strand, within GFF819 at 809.464 kb on + strand, within GFF819 at 809.464 kb on + strand, within GFF819 at 809.464 kb on + strand, within GFF819 at 809.464 kb on + strand, within GFF819 at 809.464 kb on + strand, within GFF819 at 809.464 kb on + strand, within GFF819 at 809.464 kb on + strand, within GFF819 at 809.464 kb on + strand, within GFF819 at 809.464 kb on + strand, within GFF819 at 809.464 kb on + strand, within GFF819 at 809.464 kb on + strand, within GFF819 at 809.464 kb on + strand, within GFF819 at 809.464 kb on + strand, within GFF819 at 809.464 kb on + strand, within GFF819 at 809.464 kb on + strand, within GFF819 at 809.464 kb on + strand, within GFF819 at 809.464 kb on + strand, within GFF819 at 809.464 kb on + strand, within GFF819 at 809.469 kb on + strand, within GFF819 at 809.469 kb on + strand, within GFF819 at 809.469 kb on + strand, within GFF819 at 809.469 kb on + strand, within GFF819 at 809.469 kb on + strand, within GFF819 at 809.470 kb on - strand, within GFF819 at 809.472 kb on + strand, within GFF819 at 809.472 kb on - strand, within GFF819 at 809.472 kb on - strand, within GFF819 at 809.472 kb on - strand, within GFF819 at 809.472 kb on - strand, within GFF819 at 809.472 kb on - strand, within GFF819 at 809.472 kb on - strand, within GFF819 at 809.472 kb on - strand, within GFF819 at 809.472 kb on - strand, within GFF819 at 809.472 kb on - strand, within GFF819 at 809.477 kb on - strand, within GFF819 at 809.477 kb on - strand, within GFF819 at 809.477 kb on - strand, within GFF819 at 809.477 kb on - strand, within GFF819 at 809.477 kb on - strand, within GFF819 at 809.488 kb on + strand at 809.488 kb on + strand at 809.488 kb on + strand at 809.488 kb on + strand at 809.488 kb on + strand at 809.488 kb on + strand at 809.495 kb on - strand at 809.496 kb on - strand at 809.496 kb on - strand at 809.496 kb on - strand at 809.555 kb on + strand at 809.557 kb on - strand at 809.597 kb on + strand at 809.627 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with D-Glucose and Ammonium Sulfate remove 806,584 - GFF817 0.48 -1.5 806,635 - GFF817 0.51 -1.4 807,010 - GFF817 0.75 -2.1 807,362 + -2.4 807,405 + +0.5 807,413 - +0.8 807,444 + -0.6 807,463 + -1.3 807,463 + -0.5 807,471 - +0.7 807,476 + +0.3 807,483 + -0.7 807,491 - -1.6 807,495 + -0.6 807,529 + +0.4 807,537 - +0.6 807,542 + +0.6 807,542 + -1.0 807,542 + +0.4 807,542 + -0.4 807,542 + -0.6 807,542 + +0.6 807,542 + -0.1 807,542 + +0.3 807,542 + -0.3 807,542 + -0.9 807,542 + -0.4 807,542 + -0.4 807,550 - +1.1 807,550 - +0.3 807,550 - -0.2 807,550 - -1.5 807,550 - +0.4 807,550 - -0.5 807,550 - +1.1 807,550 - -0.6 807,550 - -0.1 807,550 - -1.3 807,550 - +0.4 807,550 - +1.0 807,550 - +1.7 807,550 - -0.6 807,550 - +0.2 807,550 - +0.4 807,555 - -2.7 807,599 + -2.5 807,599 + -2.2 807,646 + -1.2 807,680 + GFF818 0.13 -1.6 807,709 - GFF818 0.15 -1.4 807,712 + GFF818 0.15 -2.7 807,725 + GFF818 0.17 -1.7 807,733 - GFF818 0.17 -1.3 807,734 + GFF818 0.17 -1.5 807,738 + GFF818 0.18 -2.4 807,746 - GFF818 0.18 -2.4 807,746 - GFF818 0.18 -2.8 807,783 + GFF818 0.22 -1.5 807,791 - GFF818 0.22 -2.1 807,809 + GFF818 0.24 -0.6 807,809 + GFF818 0.24 -4.0 807,817 - GFF818 0.24 -3.0 807,817 - GFF818 0.24 -1.7 807,833 + GFF818 0.26 -1.3 807,882 + GFF818 0.30 -2.0 807,949 - GFF818 0.36 -1.3 807,949 - GFF818 0.36 -2.1 807,970 - GFF818 0.38 -1.5 807,976 - GFF818 0.38 +0.3 807,989 + GFF818 0.39 -2.1 807,997 - GFF818 0.40 -1.6 807,997 - GFF818 0.40 -1.9 807,997 - GFF818 0.40 -2.3 807,997 - GFF818 0.40 -2.8 807,997 - GFF818 0.40 -2.7 808,058 - GFF818 0.45 -1.2 808,104 - GFF818 0.49 -1.5 808,109 + GFF818 0.50 -0.5 808,117 - GFF818 0.50 -2.4 808,138 + GFF818 0.52 -1.9 808,146 - GFF818 0.53 -1.4 808,146 - GFF818 0.53 +0.0 808,146 - GFF818 0.53 -2.8 808,195 - GFF818 0.57 -1.3 808,218 + GFF818 0.59 -2.5 808,218 + GFF818 0.59 -0.9 808,226 - GFF818 0.60 +0.1 808,271 - GFF818 0.63 -1.3 808,309 - GFF818 0.67 -1.5 808,311 + GFF818 0.67 -1.6 808,322 + GFF818 0.68 -1.2 808,330 - GFF818 0.69 +0.6 808,330 - GFF818 0.69 -1.7 808,330 - GFF818 0.69 -1.6 808,340 - GFF818 0.69 -2.6 808,340 - GFF818 0.69 -1.2 808,340 - GFF818 0.69 -1.4 808,412 - GFF818 0.76 -2.9 808,469 + GFF818 0.80 -2.9 808,472 - GFF818 0.81 -1.9 808,477 - GFF818 0.81 -1.4 808,477 - GFF818 0.81 -1.3 808,588 + -1.4 808,595 + -1.7 808,596 - -1.3 808,597 - -0.2 808,647 - -1.3 808,689 - -1.6 808,694 - -2.3 808,706 + +1.1 808,708 + -1.1 808,714 - -1.3 808,714 - -0.5 808,727 + -1.7 808,727 + -2.6 808,736 - +0.4 808,754 + -1.3 808,755 + -2.0 808,835 + +0.1 808,852 + +1.4 808,893 + -1.4 808,893 + -1.5 808,898 + -0.1 808,906 - +0.9 808,974 + GFF819 0.19 -2.4 808,982 - GFF819 0.20 -0.9 808,992 + GFF819 0.21 +0.3 808,993 - GFF819 0.22 -0.7 808,997 + GFF819 0.22 -0.9 808,997 + GFF819 0.22 -1.2 808,999 + GFF819 0.22 +0.5 809,001 + GFF819 0.23 -0.3 809,001 + GFF819 0.23 +0.2 809,005 - GFF819 0.23 -0.1 809,009 - GFF819 0.24 +0.4 809,009 - GFF819 0.24 +1.3 809,009 - GFF819 0.24 +0.5 809,034 - GFF819 0.27 -1.8 809,036 + GFF819 0.28 -0.5 809,053 + GFF819 0.30 -0.5 809,059 + GFF819 0.31 -0.6 809,059 + GFF819 0.31 +0.7 809,067 - GFF819 0.32 -1.5 809,073 + GFF819 0.33 -0.1 809,105 + GFF819 0.37 -0.8 809,127 + GFF819 0.40 -0.2 809,135 - GFF819 0.41 -0.3 809,135 - GFF819 0.41 -1.6 809,135 - GFF819 0.41 -1.3 809,135 - GFF819 0.41 -1.1 809,142 + GFF819 0.42 -0.7 809,145 - GFF819 0.43 -0.3 809,158 - GFF819 0.45 -0.3 809,165 - GFF819 0.46 +0.3 809,165 - GFF819 0.46 -1.0 809,165 - GFF819 0.46 +1.2 809,170 - GFF819 0.46 +0.4 809,179 - GFF819 0.48 +1.0 809,212 + GFF819 0.52 -0.1 809,217 + GFF819 0.53 -1.2 809,217 + GFF819 0.53 -0.6 809,217 + GFF819 0.53 +0.4 809,217 + GFF819 0.53 -0.0 809,219 + GFF819 0.53 -0.0 809,220 - GFF819 0.53 -1.7 809,225 - GFF819 0.54 +0.2 809,225 - GFF819 0.54 -0.8 809,225 - GFF819 0.54 +0.0 809,225 - GFF819 0.54 -2.2 809,225 - GFF819 0.54 +0.1 809,225 - GFF819 0.54 +0.3 809,227 - GFF819 0.54 -2.7 809,227 - GFF819 0.54 +0.4 809,235 - GFF819 0.55 +0.1 809,239 + GFF819 0.56 -0.4 809,250 - GFF819 0.57 -0.6 809,266 + GFF819 0.60 -0.6 809,266 + GFF819 0.60 -0.2 809,310 + GFF819 0.66 -1.7 809,317 + GFF819 0.67 -0.3 809,317 + GFF819 0.67 +0.3 809,317 + GFF819 0.67 -1.9 809,325 - GFF819 0.68 -0.1 809,330 + GFF819 0.69 -0.8 809,338 - GFF819 0.70 +0.1 809,338 - GFF819 0.70 +0.6 809,359 - GFF819 0.73 -1.9 809,362 + GFF819 0.73 +0.1 809,371 - GFF819 0.74 +0.1 809,383 + GFF819 0.76 +0.2 809,388 + GFF819 0.77 +0.7 809,391 - GFF819 0.77 -0.6 809,398 + GFF819 0.78 -0.0 809,398 + GFF819 0.78 -0.4 809,398 + GFF819 0.78 -1.0 809,398 + GFF819 0.78 +0.5 809,409 - GFF819 0.80 +0.2 809,409 - GFF819 0.80 +0.2 809,441 + GFF819 0.84 +0.9 809,441 + GFF819 0.84 -0.5 809,449 - GFF819 0.85 -0.6 809,449 - GFF819 0.85 -1.8 809,449 - GFF819 0.85 +1.1 809,449 - GFF819 0.85 +0.9 809,449 - GFF819 0.85 +0.9 809,449 - GFF819 0.85 +0.9 809,449 - GFF819 0.85 -0.6 809,449 - GFF819 0.85 -0.8 809,453 - GFF819 0.86 +0.6 809,462 + GFF819 0.87 +0.3 809,462 + GFF819 0.87 +0.4 809,464 + GFF819 0.87 +1.4 809,464 + GFF819 0.87 -1.0 809,464 + GFF819 0.87 +0.6 809,464 + GFF819 0.87 +0.5 809,464 + GFF819 0.87 +1.3 809,464 + GFF819 0.87 -1.6 809,464 + GFF819 0.87 -1.3 809,464 + GFF819 0.87 -0.3 809,464 + GFF819 0.87 +0.8 809,464 + GFF819 0.87 -1.9 809,464 + GFF819 0.87 +1.1 809,464 + GFF819 0.87 +0.1 809,464 + GFF819 0.87 -0.7 809,464 + GFF819 0.87 +0.5 809,464 + GFF819 0.87 +0.5 809,464 + GFF819 0.87 -0.5 809,464 + GFF819 0.87 -0.5 809,464 + GFF819 0.87 +0.9 809,464 + GFF819 0.87 -0.2 809,464 + GFF819 0.87 +1.2 809,464 + GFF819 0.87 +0.6 809,469 + GFF819 0.88 -0.0 809,469 + GFF819 0.88 -1.0 809,469 + GFF819 0.88 -1.2 809,469 + GFF819 0.88 -0.6 809,469 + GFF819 0.88 -1.0 809,470 - GFF819 0.88 -1.7 809,472 + GFF819 0.88 +0.1 809,472 - GFF819 0.88 -0.3 809,472 - GFF819 0.88 -0.1 809,472 - GFF819 0.88 -0.2 809,472 - GFF819 0.88 -1.5 809,472 - GFF819 0.88 -1.4 809,472 - GFF819 0.88 +0.4 809,472 - GFF819 0.88 -1.9 809,472 - GFF819 0.88 -0.4 809,472 - GFF819 0.88 -0.5 809,477 - GFF819 0.89 -0.0 809,477 - GFF819 0.89 -1.6 809,477 - GFF819 0.89 +0.8 809,477 - GFF819 0.89 +0.1 809,477 - GFF819 0.89 -0.3 809,488 + +1.1 809,488 + -0.3 809,488 + -0.3 809,488 + +0.5 809,488 + +0.5 809,488 + +1.0 809,495 - -1.9 809,496 - +1.2 809,496 - -0.4 809,496 - +0.3 809,555 + -1.1 809,557 - -1.2 809,597 + +0.7 809,627 + +0.4
Or see this region's nucleotide sequence