Experiment: EMM_noCarbon with D-Glucose and Ammonium Sulfate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF715 and GFF716 are separated by 87 nucleotides GFF716 and GFF717 are separated by 48 nucleotides GFF717 and GFF718 are separated by 83 nucleotides GFF718 and GFF719 are separated by 52 nucleotides
GFF715 - DNA polymerase III beta subunit (EC 2.7.7.7), at 707,975 to 709,087
GFF715
GFF716 - hypothetical protein, at 709,175 to 709,762
GFF716
GFF717 - 23S rRNA (adenine(2503)-C(2))-methyltransferase @ tRNA (adenine(37)-C(2))-methyltransferase (EC 2.1.1.192), at 709,811 to 711,070
GFF717
GFF718 - hypothetical protein, at 711,154 to 711,576
GFF718
GFF719 - hypothetical protein, at 711,629 to 712,138
GFF719
Position (kb) 709
710
711
712 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 709.170 kb on + strand at 709.194 kb on - strand at 709.199 kb on - strand at 709.223 kb on - strand at 709.238 kb on + strand, within GFF716 at 709.238 kb on + strand, within GFF716 at 709.238 kb on + strand, within GFF716 at 709.246 kb on - strand, within GFF716 at 709.246 kb on - strand, within GFF716 at 709.246 kb on - strand, within GFF716 at 709.246 kb on - strand, within GFF716 at 709.255 kb on + strand, within GFF716 at 709.264 kb on + strand, within GFF716 at 709.279 kb on + strand, within GFF716 at 709.282 kb on + strand, within GFF716 at 709.295 kb on - strand, within GFF716 at 709.295 kb on - strand, within GFF716 at 709.305 kb on - strand, within GFF716 at 709.335 kb on - strand, within GFF716 at 709.352 kb on - strand, within GFF716 at 709.360 kb on - strand, within GFF716 at 709.373 kb on + strand, within GFF716 at 709.381 kb on - strand, within GFF716 at 709.435 kb on + strand, within GFF716 at 709.435 kb on + strand, within GFF716 at 709.449 kb on - strand, within GFF716 at 709.454 kb on + strand, within GFF716 at 709.458 kb on + strand, within GFF716 at 709.464 kb on + strand, within GFF716 at 709.514 kb on + strand, within GFF716 at 709.526 kb on - strand, within GFF716 at 709.550 kb on - strand, within GFF716 at 709.550 kb on - strand, within GFF716 at 709.565 kb on - strand, within GFF716 at 709.565 kb on - strand, within GFF716 at 709.567 kb on + strand, within GFF716 at 709.575 kb on - strand, within GFF716 at 709.577 kb on - strand, within GFF716 at 709.582 kb on - strand, within GFF716 at 709.587 kb on - strand, within GFF716 at 709.606 kb on + strand, within GFF716 at 709.655 kb on + strand, within GFF716 at 709.668 kb on - strand, within GFF716 at 709.677 kb on - strand, within GFF716 at 709.695 kb on + strand, within GFF716 at 709.695 kb on + strand, within GFF716 at 709.695 kb on + strand, within GFF716 at 709.695 kb on + strand, within GFF716 at 709.695 kb on + strand, within GFF716 at 709.695 kb on + strand, within GFF716 at 709.695 kb on + strand, within GFF716 at 709.695 kb on + strand, within GFF716 at 709.695 kb on + strand, within GFF716 at 709.695 kb on + strand, within GFF716 at 709.695 kb on + strand, within GFF716 at 709.695 kb on + strand, within GFF716 at 709.695 kb on + strand, within GFF716 at 709.695 kb on + strand, within GFF716 at 709.696 kb on - strand, within GFF716 at 709.701 kb on - strand, within GFF716 at 709.703 kb on - strand, within GFF716 at 709.703 kb on - strand, within GFF716 at 709.703 kb on - strand, within GFF716 at 709.703 kb on - strand, within GFF716 at 709.703 kb on - strand, within GFF716 at 709.703 kb on - strand, within GFF716 at 709.703 kb on - strand, within GFF716 at 709.703 kb on - strand, within GFF716 at 709.703 kb on - strand, within GFF716 at 709.705 kb on + strand at 709.713 kb on - strand at 709.731 kb on + strand at 709.736 kb on + strand at 709.736 kb on + strand at 709.736 kb on + strand at 709.736 kb on + strand at 709.741 kb on + strand at 709.744 kb on - strand at 709.744 kb on - strand at 709.744 kb on - strand at 709.744 kb on - strand at 709.744 kb on - strand at 709.744 kb on - strand at 709.749 kb on - strand at 709.749 kb on - strand at 709.759 kb on - strand at 709.759 kb on - strand at 709.808 kb on + strand at 709.808 kb on + strand at 709.808 kb on + strand at 709.808 kb on + strand at 709.809 kb on - strand at 709.852 kb on + strand at 709.878 kb on - strand at 709.903 kb on + strand at 709.911 kb on + strand at 709.924 kb on - strand at 709.924 kb on - strand at 709.933 kb on + strand at 709.933 kb on + strand at 709.933 kb on + strand at 709.933 kb on + strand at 709.941 kb on - strand, within GFF717 at 709.941 kb on - strand, within GFF717 at 709.941 kb on - strand, within GFF717 at 709.941 kb on - strand, within GFF717 at 709.941 kb on - strand, within GFF717 at 709.941 kb on - strand, within GFF717 at 709.941 kb on - strand, within GFF717 at 709.941 kb on - strand, within GFF717 at 709.941 kb on - strand, within GFF717 at 709.941 kb on - strand, within GFF717 at 709.941 kb on - strand, within GFF717 at 709.941 kb on - strand, within GFF717 at 709.941 kb on - strand, within GFF717 at 709.954 kb on + strand, within GFF717 at 709.954 kb on + strand, within GFF717 at 709.981 kb on + strand, within GFF717 at 710.051 kb on - strand, within GFF717 at 710.053 kb on + strand, within GFF717 at 710.061 kb on - strand, within GFF717 at 710.076 kb on - strand, within GFF717 at 710.098 kb on - strand, within GFF717 at 710.107 kb on + strand, within GFF717 at 710.122 kb on + strand, within GFF717 at 710.155 kb on - strand, within GFF717 at 710.168 kb on + strand, within GFF717 at 710.181 kb on - strand, within GFF717 at 710.241 kb on - strand, within GFF717 at 710.258 kb on - strand, within GFF717 at 710.260 kb on + strand, within GFF717 at 710.278 kb on - strand, within GFF717 at 710.292 kb on + strand, within GFF717 at 710.300 kb on - strand, within GFF717 at 710.304 kb on - strand, within GFF717 at 710.320 kb on + strand, within GFF717 at 710.320 kb on + strand, within GFF717 at 710.320 kb on + strand, within GFF717 at 710.320 kb on + strand, within GFF717 at 710.320 kb on + strand, within GFF717 at 710.328 kb on - strand, within GFF717 at 710.339 kb on + strand, within GFF717 at 710.363 kb on + strand, within GFF717 at 710.367 kb on - strand, within GFF717 at 710.367 kb on - strand, within GFF717 at 710.383 kb on + strand, within GFF717 at 710.393 kb on + strand, within GFF717 at 710.417 kb on - strand, within GFF717 at 710.417 kb on - strand, within GFF717 at 710.467 kb on + strand, within GFF717 at 710.467 kb on + strand, within GFF717 at 710.467 kb on + strand, within GFF717 at 710.475 kb on - strand, within GFF717 at 710.520 kb on + strand, within GFF717 at 710.522 kb on - strand, within GFF717 at 710.527 kb on - strand, within GFF717 at 710.528 kb on - strand, within GFF717 at 710.567 kb on + strand, within GFF717 at 710.600 kb on + strand, within GFF717 at 710.600 kb on + strand, within GFF717 at 710.601 kb on + strand, within GFF717 at 710.634 kb on + strand, within GFF717 at 710.642 kb on - strand, within GFF717 at 710.679 kb on + strand, within GFF717 at 710.687 kb on + strand, within GFF717 at 710.693 kb on + strand, within GFF717 at 710.693 kb on + strand, within GFF717 at 710.693 kb on + strand, within GFF717 at 710.700 kb on - strand, within GFF717 at 710.700 kb on - strand, within GFF717 at 710.701 kb on - strand, within GFF717 at 710.711 kb on - strand, within GFF717 at 710.711 kb on - strand, within GFF717 at 710.719 kb on + strand, within GFF717 at 710.719 kb on + strand, within GFF717 at 710.721 kb on - strand, within GFF717 at 710.727 kb on - strand, within GFF717 at 710.764 kb on + strand, within GFF717 at 710.790 kb on + strand, within GFF717 at 710.798 kb on - strand, within GFF717 at 710.835 kb on + strand, within GFF717 at 710.836 kb on + strand, within GFF717 at 710.841 kb on + strand, within GFF717 at 710.841 kb on + strand, within GFF717 at 710.841 kb on + strand, within GFF717 at 710.849 kb on - strand, within GFF717 at 710.851 kb on + strand, within GFF717 at 710.864 kb on - strand, within GFF717 at 710.881 kb on + strand, within GFF717 at 710.897 kb on - strand, within GFF717 at 710.902 kb on - strand, within GFF717 at 710.955 kb on - strand at 710.964 kb on + strand at 710.982 kb on + strand at 710.990 kb on - strand at 710.990 kb on - strand at 710.990 kb on - strand at 711.049 kb on + strand at 711.064 kb on + strand at 711.119 kb on - strand at 711.202 kb on + strand, within GFF718 at 711.207 kb on + strand, within GFF718 at 711.207 kb on + strand, within GFF718 at 711.227 kb on + strand, within GFF718 at 711.254 kb on + strand, within GFF718 at 711.254 kb on + strand, within GFF718 at 711.262 kb on - strand, within GFF718 at 711.335 kb on - strand, within GFF718 at 711.336 kb on + strand, within GFF718 at 711.356 kb on + strand, within GFF718 at 711.359 kb on + strand, within GFF718 at 711.361 kb on + strand, within GFF718 at 711.373 kb on + strand, within GFF718 at 711.381 kb on - strand, within GFF718 at 711.390 kb on - strand, within GFF718 at 711.400 kb on - strand, within GFF718 at 711.402 kb on - strand, within GFF718 at 711.402 kb on - strand, within GFF718 at 711.480 kb on + strand, within GFF718 at 711.480 kb on - strand, within GFF718 at 711.481 kb on + strand, within GFF718 at 711.607 kb on + strand at 711.758 kb on + strand, within GFF719 at 711.812 kb on + strand, within GFF719 at 712.010 kb on + strand, within GFF719 at 712.028 kb on + strand, within GFF719 at 712.053 kb on + strand, within GFF719
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with D-Glucose and Ammonium Sulfate remove 709,170 + -0.2 709,194 - -2.8 709,199 - +1.0 709,223 - +0.6 709,238 + GFF716 0.11 -0.9 709,238 + GFF716 0.11 -0.5 709,238 + GFF716 0.11 -0.3 709,246 - GFF716 0.12 +0.7 709,246 - GFF716 0.12 -0.4 709,246 - GFF716 0.12 +0.8 709,246 - GFF716 0.12 -0.3 709,255 + GFF716 0.14 +0.7 709,264 + GFF716 0.15 -0.0 709,279 + GFF716 0.18 +0.1 709,282 + GFF716 0.18 -0.9 709,295 - GFF716 0.20 -0.9 709,295 - GFF716 0.20 -0.1 709,305 - GFF716 0.22 -0.3 709,335 - GFF716 0.27 +0.5 709,352 - GFF716 0.30 -0.2 709,360 - GFF716 0.31 -0.0 709,373 + GFF716 0.34 -0.7 709,381 - GFF716 0.35 -0.3 709,435 + GFF716 0.44 +0.7 709,435 + GFF716 0.44 +0.3 709,449 - GFF716 0.47 +1.3 709,454 + GFF716 0.47 +0.2 709,458 + GFF716 0.48 -1.2 709,464 + GFF716 0.49 -0.9 709,514 + GFF716 0.58 +0.2 709,526 - GFF716 0.60 -0.9 709,550 - GFF716 0.64 +0.3 709,550 - GFF716 0.64 -0.5 709,565 - GFF716 0.66 +0.6 709,565 - GFF716 0.66 +0.8 709,567 + GFF716 0.67 +0.0 709,575 - GFF716 0.68 +0.7 709,577 - GFF716 0.68 +0.7 709,582 - GFF716 0.69 +0.6 709,587 - GFF716 0.70 +1.0 709,606 + GFF716 0.73 -0.1 709,655 + GFF716 0.82 +0.7 709,668 - GFF716 0.84 -0.2 709,677 - GFF716 0.85 +0.3 709,695 + GFF716 0.88 +0.7 709,695 + GFF716 0.88 -0.2 709,695 + GFF716 0.88 -0.6 709,695 + GFF716 0.88 -0.0 709,695 + GFF716 0.88 +0.6 709,695 + GFF716 0.88 +0.8 709,695 + GFF716 0.88 -0.3 709,695 + GFF716 0.88 +0.1 709,695 + GFF716 0.88 +1.2 709,695 + GFF716 0.88 +0.1 709,695 + GFF716 0.88 +0.3 709,695 + GFF716 0.88 -0.2 709,695 + GFF716 0.88 +2.6 709,695 + GFF716 0.88 +1.3 709,696 - GFF716 0.89 +0.6 709,701 - GFF716 0.89 +0.4 709,703 - GFF716 0.90 +2.0 709,703 - GFF716 0.90 -0.1 709,703 - GFF716 0.90 +0.5 709,703 - GFF716 0.90 -0.0 709,703 - GFF716 0.90 -1.1 709,703 - GFF716 0.90 -0.1 709,703 - GFF716 0.90 +0.7 709,703 - GFF716 0.90 +0.5 709,703 - GFF716 0.90 -1.5 709,705 + +0.1 709,713 - +0.8 709,731 + -0.7 709,736 + +0.2 709,736 + -0.5 709,736 + +0.7 709,736 + -1.1 709,741 + -2.5 709,744 - +0.6 709,744 - +0.3 709,744 - +1.4 709,744 - +2.0 709,744 - +1.0 709,744 - -1.5 709,749 - -0.8 709,749 - -0.3 709,759 - +0.6 709,759 - +0.0 709,808 + +0.1 709,808 + +0.8 709,808 + +0.1 709,808 + -2.0 709,809 - -0.8 709,852 + +0.2 709,878 - +0.3 709,903 + +1.1 709,911 + +1.2 709,924 - +0.6 709,924 - -1.4 709,933 + -0.2 709,933 + +0.3 709,933 + -1.0 709,933 + -0.6 709,941 - GFF717 0.10 +2.7 709,941 - GFF717 0.10 +0.1 709,941 - GFF717 0.10 +0.7 709,941 - GFF717 0.10 +0.3 709,941 - GFF717 0.10 +0.5 709,941 - GFF717 0.10 -0.2 709,941 - GFF717 0.10 +1.0 709,941 - GFF717 0.10 +0.2 709,941 - GFF717 0.10 +0.6 709,941 - GFF717 0.10 -1.1 709,941 - GFF717 0.10 -0.1 709,941 - GFF717 0.10 +0.1 709,941 - GFF717 0.10 +0.4 709,954 + GFF717 0.11 -1.1 709,954 + GFF717 0.11 +0.6 709,981 + GFF717 0.13 -1.4 710,051 - GFF717 0.19 -0.9 710,053 + GFF717 0.19 -0.3 710,061 - GFF717 0.20 +0.2 710,076 - GFF717 0.21 +0.1 710,098 - GFF717 0.23 +0.2 710,107 + GFF717 0.23 -0.1 710,122 + GFF717 0.25 +0.2 710,155 - GFF717 0.27 -0.2 710,168 + GFF717 0.28 +0.6 710,181 - GFF717 0.29 -0.2 710,241 - GFF717 0.34 +0.7 710,258 - GFF717 0.35 -1.1 710,260 + GFF717 0.36 -0.4 710,278 - GFF717 0.37 +1.0 710,292 + GFF717 0.38 -1.3 710,300 - GFF717 0.39 +0.2 710,304 - GFF717 0.39 +0.4 710,320 + GFF717 0.40 +0.2 710,320 + GFF717 0.40 +1.6 710,320 + GFF717 0.40 -0.2 710,320 + GFF717 0.40 +1.9 710,320 + GFF717 0.40 -0.5 710,328 - GFF717 0.41 -0.9 710,339 + GFF717 0.42 +0.1 710,363 + GFF717 0.44 +1.2 710,367 - GFF717 0.44 -0.9 710,367 - GFF717 0.44 -0.4 710,383 + GFF717 0.45 -1.7 710,393 + GFF717 0.46 +0.5 710,417 - GFF717 0.48 +0.1 710,417 - GFF717 0.48 -0.4 710,467 + GFF717 0.52 -0.3 710,467 + GFF717 0.52 -1.7 710,467 + GFF717 0.52 -1.8 710,475 - GFF717 0.53 -1.0 710,520 + GFF717 0.56 -0.1 710,522 - GFF717 0.56 +0.8 710,527 - GFF717 0.57 +0.3 710,528 - GFF717 0.57 -0.2 710,567 + GFF717 0.60 +0.6 710,600 + GFF717 0.63 -0.3 710,600 + GFF717 0.63 +0.1 710,601 + GFF717 0.63 +2.4 710,634 + GFF717 0.65 -1.3 710,642 - GFF717 0.66 -1.0 710,679 + GFF717 0.69 +1.5 710,687 + GFF717 0.70 +1.0 710,693 + GFF717 0.70 +0.9 710,693 + GFF717 0.70 -1.9 710,693 + GFF717 0.70 -0.4 710,700 - GFF717 0.71 -0.3 710,700 - GFF717 0.71 +1.4 710,701 - GFF717 0.71 -1.5 710,711 - GFF717 0.71 -0.4 710,711 - GFF717 0.71 +1.9 710,719 + GFF717 0.72 -0.0 710,719 + GFF717 0.72 +1.2 710,721 - GFF717 0.72 +0.6 710,727 - GFF717 0.73 +0.6 710,764 + GFF717 0.76 +0.5 710,790 + GFF717 0.78 +0.2 710,798 - GFF717 0.78 -0.6 710,835 + GFF717 0.81 -0.7 710,836 + GFF717 0.81 +1.7 710,841 + GFF717 0.82 +0.2 710,841 + GFF717 0.82 +1.2 710,841 + GFF717 0.82 -1.5 710,849 - GFF717 0.82 -1.7 710,851 + GFF717 0.83 -1.2 710,864 - GFF717 0.84 -1.4 710,881 + GFF717 0.85 -1.6 710,897 - GFF717 0.86 -0.2 710,902 - GFF717 0.87 +0.4 710,955 - +1.0 710,964 + +0.8 710,982 + -1.8 710,990 - -1.3 710,990 - -2.3 710,990 - +0.3 711,049 + +0.4 711,064 + -1.3 711,119 - -0.8 711,202 + GFF718 0.11 -1.3 711,207 + GFF718 0.13 -0.3 711,207 + GFF718 0.13 -2.2 711,227 + GFF718 0.17 +0.3 711,254 + GFF718 0.24 +1.1 711,254 + GFF718 0.24 +0.4 711,262 - GFF718 0.26 +0.3 711,335 - GFF718 0.43 +0.3 711,336 + GFF718 0.43 +1.4 711,356 + GFF718 0.48 +0.7 711,359 + GFF718 0.48 -0.1 711,361 + GFF718 0.49 -0.8 711,373 + GFF718 0.52 -0.8 711,381 - GFF718 0.54 -0.9 711,390 - GFF718 0.56 +0.8 711,400 - GFF718 0.58 +0.0 711,402 - GFF718 0.59 +0.0 711,402 - GFF718 0.59 +0.8 711,480 + GFF718 0.77 -1.1 711,480 - GFF718 0.77 +0.3 711,481 + GFF718 0.77 -1.3 711,607 + +1.7 711,758 + GFF719 0.25 +0.6 711,812 + GFF719 0.36 -0.4 712,010 + GFF719 0.75 -1.1 712,028 + GFF719 0.78 -1.2 712,053 + GFF719 0.83 -1.1
Or see this region's nucleotide sequence