Experiment: EMM_noCarbon with D-Glucose and Ammonium Sulfate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF646 and GFF647 are separated by 138 nucleotides GFF647 and GFF648 overlap by 4 nucleotides GFF648 and GFF649 are separated by 9 nucleotides
GFF646 - Aspartate aminotransferase (EC 2.6.1.1), at 644,843 to 646,054
GFF646
GFF647 - hypothetical protein, at 646,193 to 646,669
GFF647
GFF648 - Uncharacterized aldehyde oxidase, 2Fe-2S subunit, at 646,666 to 647,217
GFF648
GFF649 - hypothetical protein, at 647,227 to 648,036
GFF649
Position (kb) 646
647 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 645.235 kb on - strand, within GFF646 at 645.257 kb on + strand, within GFF646 at 645.272 kb on + strand, within GFF646 at 645.322 kb on + strand, within GFF646 at 645.322 kb on + strand, within GFF646 at 645.322 kb on + strand, within GFF646 at 645.323 kb on + strand, within GFF646 at 645.337 kb on - strand, within GFF646 at 645.350 kb on - strand, within GFF646 at 645.399 kb on - strand, within GFF646 at 645.439 kb on + strand, within GFF646 at 645.439 kb on + strand, within GFF646 at 645.447 kb on - strand, within GFF646 at 645.447 kb on - strand, within GFF646 at 645.447 kb on - strand, within GFF646 at 645.448 kb on + strand, within GFF646 at 645.459 kb on - strand, within GFF646 at 645.488 kb on + strand, within GFF646 at 645.498 kb on + strand, within GFF646 at 645.506 kb on - strand, within GFF646 at 645.506 kb on - strand, within GFF646 at 645.553 kb on + strand, within GFF646 at 645.553 kb on + strand, within GFF646 at 645.553 kb on + strand, within GFF646 at 645.553 kb on + strand, within GFF646 at 645.553 kb on + strand, within GFF646 at 645.553 kb on + strand, within GFF646 at 645.553 kb on + strand, within GFF646 at 645.556 kb on - strand, within GFF646 at 645.561 kb on - strand, within GFF646 at 645.561 kb on - strand, within GFF646 at 645.561 kb on - strand, within GFF646 at 645.596 kb on - strand, within GFF646 at 645.596 kb on - strand, within GFF646 at 645.632 kb on + strand, within GFF646 at 645.679 kb on + strand, within GFF646 at 645.697 kb on - strand, within GFF646 at 645.701 kb on + strand, within GFF646 at 645.714 kb on - strand, within GFF646 at 645.731 kb on + strand, within GFF646 at 645.741 kb on - strand, within GFF646 at 645.817 kb on - strand, within GFF646 at 645.832 kb on - strand, within GFF646 at 645.875 kb on + strand, within GFF646 at 645.903 kb on + strand, within GFF646 at 645.925 kb on + strand, within GFF646 at 645.935 kb on + strand at 645.943 kb on - strand at 645.943 kb on - strand at 645.943 kb on - strand at 645.957 kb on - strand at 645.960 kb on + strand at 645.963 kb on - strand at 646.109 kb on - strand at 646.136 kb on + strand at 646.136 kb on + strand at 646.161 kb on + strand at 646.162 kb on + strand at 646.168 kb on + strand at 646.169 kb on - strand at 646.174 kb on - strand at 646.218 kb on - strand at 646.231 kb on - strand at 646.244 kb on - strand, within GFF647 at 646.279 kb on - strand, within GFF647 at 646.280 kb on + strand, within GFF647 at 646.280 kb on + strand, within GFF647 at 646.288 kb on - strand, within GFF647 at 646.291 kb on - strand, within GFF647 at 646.324 kb on + strand, within GFF647 at 646.342 kb on - strand, within GFF647 at 646.360 kb on + strand, within GFF647 at 646.374 kb on + strand, within GFF647 at 646.375 kb on + strand, within GFF647 at 646.375 kb on - strand, within GFF647 at 646.375 kb on - strand, within GFF647 at 646.382 kb on - strand, within GFF647 at 646.382 kb on - strand, within GFF647 at 646.386 kb on + strand, within GFF647 at 646.386 kb on - strand, within GFF647 at 646.416 kb on - strand, within GFF647 at 646.427 kb on + strand, within GFF647 at 646.435 kb on + strand, within GFF647 at 646.441 kb on + strand, within GFF647 at 646.448 kb on + strand, within GFF647 at 646.456 kb on - strand, within GFF647 at 646.475 kb on - strand, within GFF647 at 646.506 kb on + strand, within GFF647 at 646.511 kb on + strand, within GFF647 at 646.511 kb on + strand, within GFF647 at 646.514 kb on - strand, within GFF647 at 646.519 kb on - strand, within GFF647 at 646.519 kb on - strand, within GFF647 at 646.519 kb on - strand, within GFF647 at 646.519 kb on - strand, within GFF647 at 646.524 kb on - strand, within GFF647 at 646.524 kb on - strand, within GFF647 at 646.532 kb on + strand, within GFF647 at 646.540 kb on - strand, within GFF647 at 646.540 kb on - strand, within GFF647 at 646.546 kb on + strand, within GFF647 at 646.553 kb on + strand, within GFF647 at 646.561 kb on - strand, within GFF647 at 646.613 kb on + strand, within GFF647 at 646.613 kb on + strand, within GFF647 at 646.619 kb on - strand, within GFF647 at 646.627 kb on - strand at 646.638 kb on + strand at 646.652 kb on - strand at 646.659 kb on + strand at 646.679 kb on - strand at 646.704 kb on - strand at 646.719 kb on - strand at 646.723 kb on + strand, within GFF648 at 646.723 kb on + strand, within GFF648 at 646.723 kb on + strand, within GFF648 at 646.728 kb on + strand, within GFF648 at 646.728 kb on + strand, within GFF648 at 646.728 kb on + strand, within GFF648 at 646.728 kb on + strand, within GFF648 at 646.728 kb on + strand, within GFF648 at 646.728 kb on + strand, within GFF648 at 646.728 kb on + strand, within GFF648 at 646.728 kb on + strand, within GFF648 at 646.731 kb on - strand, within GFF648 at 646.731 kb on - strand, within GFF648 at 646.736 kb on - strand, within GFF648 at 646.736 kb on - strand, within GFF648 at 646.736 kb on - strand, within GFF648 at 646.736 kb on - strand, within GFF648 at 646.736 kb on - strand, within GFF648 at 646.736 kb on - strand, within GFF648 at 646.736 kb on - strand, within GFF648 at 646.736 kb on - strand, within GFF648 at 646.736 kb on - strand, within GFF648 at 646.736 kb on - strand, within GFF648 at 646.756 kb on - strand, within GFF648 at 646.828 kb on + strand, within GFF648 at 647.014 kb on - strand, within GFF648 at 647.036 kb on - strand, within GFF648 at 647.056 kb on - strand, within GFF648 at 647.069 kb on + strand, within GFF648 at 647.073 kb on - strand, within GFF648 at 647.077 kb on - strand, within GFF648 at 647.077 kb on - strand, within GFF648 at 647.117 kb on + strand, within GFF648 at 647.117 kb on + strand, within GFF648 at 647.121 kb on + strand, within GFF648 at 647.145 kb on - strand, within GFF648 at 647.213 kb on - strand at 647.213 kb on - strand at 647.220 kb on - strand at 647.231 kb on + strand at 647.257 kb on + strand at 647.257 kb on + strand at 647.257 kb on + strand at 647.257 kb on + strand at 647.257 kb on + strand at 647.265 kb on - strand at 647.265 kb on - strand at 647.265 kb on - strand at 647.265 kb on - strand at 647.265 kb on - strand at 647.265 kb on - strand at 647.265 kb on - strand at 647.265 kb on - strand at 647.265 kb on - strand at 647.265 kb on - strand at 647.265 kb on - strand at 647.265 kb on - strand at 647.265 kb on - strand at 647.265 kb on - strand at 647.265 kb on - strand at 647.265 kb on - strand at 647.265 kb on - strand at 647.265 kb on - strand at 647.265 kb on - strand at 647.265 kb on - strand at 647.280 kb on - strand at 647.282 kb on - strand at 647.284 kb on + strand at 647.285 kb on - strand at 647.306 kb on + strand at 647.315 kb on + strand, within GFF649 at 647.362 kb on + strand, within GFF649 at 647.381 kb on - strand, within GFF649 at 647.429 kb on + strand, within GFF649 at 647.434 kb on + strand, within GFF649 at 647.487 kb on + strand, within GFF649 at 647.487 kb on + strand, within GFF649 at 647.493 kb on + strand, within GFF649 at 647.503 kb on + strand, within GFF649 at 647.511 kb on - strand, within GFF649 at 647.524 kb on + strand, within GFF649 at 647.542 kb on - strand, within GFF649 at 647.622 kb on + strand, within GFF649 at 647.627 kb on + strand, within GFF649 at 647.627 kb on + strand, within GFF649 at 647.627 kb on + strand, within GFF649 at 647.635 kb on - strand, within GFF649 at 647.661 kb on - strand, within GFF649 at 647.668 kb on + strand, within GFF649 at 647.669 kb on + strand, within GFF649
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with D-Glucose and Ammonium Sulfate remove 645,235 - GFF646 0.32 -0.8 645,257 + GFF646 0.34 -0.6 645,272 + GFF646 0.35 -1.5 645,322 + GFF646 0.40 -0.8 645,322 + GFF646 0.40 -1.8 645,322 + GFF646 0.40 -0.2 645,323 + GFF646 0.40 -2.0 645,337 - GFF646 0.41 +0.7 645,350 - GFF646 0.42 +0.2 645,399 - GFF646 0.46 -0.8 645,439 + GFF646 0.49 +0.7 645,439 + GFF646 0.49 -0.9 645,447 - GFF646 0.50 -1.0 645,447 - GFF646 0.50 -1.6 645,447 - GFF646 0.50 -1.0 645,448 + GFF646 0.50 -1.2 645,459 - GFF646 0.51 -0.7 645,488 + GFF646 0.53 +1.6 645,498 + GFF646 0.54 -1.5 645,506 - GFF646 0.55 -0.9 645,506 - GFF646 0.55 -1.1 645,553 + GFF646 0.59 -1.8 645,553 + GFF646 0.59 -1.9 645,553 + GFF646 0.59 -0.2 645,553 + GFF646 0.59 -2.1 645,553 + GFF646 0.59 -2.2 645,553 + GFF646 0.59 +0.3 645,553 + GFF646 0.59 -0.9 645,556 - GFF646 0.59 +1.4 645,561 - GFF646 0.59 -1.0 645,561 - GFF646 0.59 -1.6 645,561 - GFF646 0.59 -1.7 645,596 - GFF646 0.62 -1.5 645,596 - GFF646 0.62 -0.4 645,632 + GFF646 0.65 -2.8 645,679 + GFF646 0.69 -2.4 645,697 - GFF646 0.70 +0.8 645,701 + GFF646 0.71 -1.6 645,714 - GFF646 0.72 +0.0 645,731 + GFF646 0.73 +0.4 645,741 - GFF646 0.74 +0.8 645,817 - GFF646 0.80 +0.0 645,832 - GFF646 0.82 -0.2 645,875 + GFF646 0.85 +1.4 645,903 + GFF646 0.87 -1.7 645,925 + GFF646 0.89 -1.5 645,935 + -1.4 645,943 - -2.9 645,943 - -0.9 645,943 - -1.8 645,957 - -1.5 645,960 + -1.4 645,963 - -3.2 646,109 - -0.9 646,136 + -0.0 646,136 + -1.0 646,161 + -0.1 646,162 + +1.4 646,168 + +0.4 646,169 - +0.8 646,174 - -0.1 646,218 - -1.0 646,231 - +0.4 646,244 - GFF647 0.11 +1.0 646,279 - GFF647 0.18 -0.8 646,280 + GFF647 0.18 -1.4 646,280 + GFF647 0.18 -1.9 646,288 - GFF647 0.20 -0.4 646,291 - GFF647 0.21 +0.8 646,324 + GFF647 0.27 +0.2 646,342 - GFF647 0.31 -0.0 646,360 + GFF647 0.35 +0.2 646,374 + GFF647 0.38 -0.4 646,375 + GFF647 0.38 +0.2 646,375 - GFF647 0.38 -2.0 646,375 - GFF647 0.38 -0.2 646,382 - GFF647 0.40 -0.4 646,382 - GFF647 0.40 +0.0 646,386 + GFF647 0.40 +1.1 646,386 - GFF647 0.40 -2.1 646,416 - GFF647 0.47 +0.2 646,427 + GFF647 0.49 -0.2 646,435 + GFF647 0.51 -0.8 646,441 + GFF647 0.52 +0.6 646,448 + GFF647 0.53 -1.2 646,456 - GFF647 0.55 -0.4 646,475 - GFF647 0.59 +1.4 646,506 + GFF647 0.66 +0.0 646,511 + GFF647 0.67 -2.3 646,511 + GFF647 0.67 +0.2 646,514 - GFF647 0.67 -1.1 646,519 - GFF647 0.68 +0.3 646,519 - GFF647 0.68 +0.4 646,519 - GFF647 0.68 +1.0 646,519 - GFF647 0.68 +0.7 646,524 - GFF647 0.69 -1.0 646,524 - GFF647 0.69 -0.8 646,532 + GFF647 0.71 -1.6 646,540 - GFF647 0.73 -1.0 646,540 - GFF647 0.73 +2.4 646,546 + GFF647 0.74 +1.4 646,553 + GFF647 0.75 -0.2 646,561 - GFF647 0.77 -0.8 646,613 + GFF647 0.88 -0.7 646,613 + GFF647 0.88 +0.5 646,619 - GFF647 0.89 +0.4 646,627 - -0.2 646,638 + -0.1 646,652 - -0.2 646,659 + +0.9 646,679 - +0.9 646,704 - +1.0 646,719 - -0.1 646,723 + GFF648 0.10 -0.1 646,723 + GFF648 0.10 +0.6 646,723 + GFF648 0.10 -0.4 646,728 + GFF648 0.11 +0.3 646,728 + GFF648 0.11 +0.3 646,728 + GFF648 0.11 +0.4 646,728 + GFF648 0.11 +0.4 646,728 + GFF648 0.11 -0.6 646,728 + GFF648 0.11 -0.7 646,728 + GFF648 0.11 -0.1 646,728 + GFF648 0.11 -1.3 646,731 - GFF648 0.12 -1.4 646,731 - GFF648 0.12 -0.1 646,736 - GFF648 0.13 +0.5 646,736 - GFF648 0.13 +0.8 646,736 - GFF648 0.13 +0.0 646,736 - GFF648 0.13 +1.1 646,736 - GFF648 0.13 -0.7 646,736 - GFF648 0.13 -0.3 646,736 - GFF648 0.13 -0.1 646,736 - GFF648 0.13 -0.9 646,736 - GFF648 0.13 +0.5 646,736 - GFF648 0.13 -1.1 646,756 - GFF648 0.16 -0.6 646,828 + GFF648 0.29 -0.2 647,014 - GFF648 0.63 +1.6 647,036 - GFF648 0.67 -1.1 647,056 - GFF648 0.71 -0.2 647,069 + GFF648 0.73 +0.5 647,073 - GFF648 0.74 -0.2 647,077 - GFF648 0.74 +1.4 647,077 - GFF648 0.74 -1.5 647,117 + GFF648 0.82 +0.4 647,117 + GFF648 0.82 -0.8 647,121 + GFF648 0.82 -0.7 647,145 - GFF648 0.87 +0.6 647,213 - +0.1 647,213 - -0.4 647,220 - +0.5 647,231 + +2.6 647,257 + -0.4 647,257 + -0.4 647,257 + -0.8 647,257 + -0.3 647,257 + +0.5 647,265 - -0.4 647,265 - -0.9 647,265 - -1.3 647,265 - -1.3 647,265 - -0.7 647,265 - +0.9 647,265 - +1.1 647,265 - -0.3 647,265 - +0.8 647,265 - -0.3 647,265 - +0.1 647,265 - -0.5 647,265 - +0.6 647,265 - -0.6 647,265 - -1.2 647,265 - -0.6 647,265 - -2.0 647,265 - -0.4 647,265 - -0.1 647,265 - +0.9 647,280 - -1.1 647,282 - -1.6 647,284 + -0.5 647,285 - +0.4 647,306 + -1.7 647,315 + GFF649 0.11 +0.7 647,362 + GFF649 0.17 +1.1 647,381 - GFF649 0.19 +0.5 647,429 + GFF649 0.25 +0.8 647,434 + GFF649 0.26 +0.4 647,487 + GFF649 0.32 -2.8 647,487 + GFF649 0.32 +0.7 647,493 + GFF649 0.33 +1.6 647,503 + GFF649 0.34 -1.1 647,511 - GFF649 0.35 +0.7 647,524 + GFF649 0.37 +0.0 647,542 - GFF649 0.39 +0.4 647,622 + GFF649 0.49 -0.7 647,627 + GFF649 0.49 -1.4 647,627 + GFF649 0.49 -1.2 647,627 + GFF649 0.49 -3.0 647,635 - GFF649 0.50 -0.0 647,661 - GFF649 0.54 +0.1 647,668 + GFF649 0.54 -2.0 647,669 + GFF649 0.55 -2.3
Or see this region's nucleotide sequence