Experiment: EMM_noCarbon with D-Glucose and Ammonium Sulfate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF528 and GFF529 are separated by 5 nucleotides GFF529 and GFF530 are separated by 38 nucleotides GFF530 and GFF531 are separated by 14 nucleotides
GFF528 - Muconate cycloisomerase (EC 5.5.1.1), at 525,878 to 527,035
GFF528
GFF529 - Muconolactone isomerase (EC 5.3.3.4), at 527,041 to 527,331
GFF529
GFF530 - Catechol 1,2-dioxygenase (EC 1.13.11.1), at 527,370 to 528,296
GFF530
GFF531 - Benzoate 1,2-dioxygenase alpha subunit (EC 1.14.12.10), at 528,311 to 529,672
GFF531
Position (kb)
527
528
529 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 526.405 kb on - strand, within GFF528 at 526.405 kb on - strand, within GFF528 at 526.406 kb on + strand, within GFF528 at 526.437 kb on - strand, within GFF528 at 526.472 kb on + strand, within GFF528 at 526.472 kb on + strand, within GFF528 at 526.472 kb on + strand, within GFF528 at 526.482 kb on + strand, within GFF528 at 526.495 kb on + strand, within GFF528 at 526.501 kb on + strand, within GFF528 at 526.517 kb on - strand, within GFF528 at 526.519 kb on + strand, within GFF528 at 526.538 kb on - strand, within GFF528 at 526.602 kb on - strand, within GFF528 at 526.642 kb on - strand, within GFF528 at 526.652 kb on - strand, within GFF528 at 526.659 kb on + strand, within GFF528 at 526.667 kb on - strand, within GFF528 at 526.688 kb on + strand, within GFF528 at 526.722 kb on - strand, within GFF528 at 526.831 kb on + strand, within GFF528 at 526.878 kb on - strand, within GFF528 at 526.904 kb on - strand, within GFF528 at 526.938 kb on + strand at 526.938 kb on + strand at 526.938 kb on + strand at 526.938 kb on + strand at 526.943 kb on + strand at 526.943 kb on + strand at 526.946 kb on - strand at 526.946 kb on - strand at 526.946 kb on - strand at 526.946 kb on - strand at 526.951 kb on - strand at 526.951 kb on - strand at 526.951 kb on - strand at 526.956 kb on + strand at 526.963 kb on - strand at 526.964 kb on - strand at 526.964 kb on - strand at 526.964 kb on - strand at 526.966 kb on + strand at 526.974 kb on - strand at 527.032 kb on - strand at 527.036 kb on + strand at 527.044 kb on - strand at 527.044 kb on - strand at 527.106 kb on + strand, within GFF529 at 527.114 kb on - strand, within GFF529 at 527.114 kb on - strand, within GFF529 at 527.171 kb on + strand, within GFF529 at 527.197 kb on + strand, within GFF529 at 527.217 kb on + strand, within GFF529 at 527.217 kb on + strand, within GFF529 at 527.217 kb on + strand, within GFF529 at 527.225 kb on - strand, within GFF529 at 527.230 kb on - strand, within GFF529 at 527.263 kb on + strand, within GFF529 at 527.294 kb on + strand, within GFF529 at 527.300 kb on - strand, within GFF529 at 527.328 kb on - strand at 527.334 kb on + strand at 527.353 kb on + strand at 527.361 kb on - strand at 527.361 kb on - strand at 527.377 kb on - strand at 527.409 kb on + strand at 527.409 kb on + strand at 527.427 kb on + strand at 527.464 kb on - strand, within GFF530 at 527.481 kb on + strand, within GFF530 at 527.488 kb on - strand, within GFF530 at 527.489 kb on - strand, within GFF530 at 527.517 kb on - strand, within GFF530 at 527.526 kb on + strand, within GFF530 at 527.531 kb on + strand, within GFF530 at 527.534 kb on + strand, within GFF530 at 527.534 kb on - strand, within GFF530 at 527.540 kb on - strand, within GFF530 at 527.578 kb on + strand, within GFF530 at 527.578 kb on + strand, within GFF530 at 527.586 kb on - strand, within GFF530 at 527.601 kb on - strand, within GFF530 at 527.613 kb on - strand, within GFF530 at 527.627 kb on + strand, within GFF530 at 527.635 kb on - strand, within GFF530 at 527.693 kb on + strand, within GFF530 at 527.693 kb on + strand, within GFF530 at 527.701 kb on - strand, within GFF530 at 527.723 kb on + strand, within GFF530 at 527.766 kb on + strand, within GFF530 at 527.766 kb on + strand, within GFF530 at 527.768 kb on + strand, within GFF530 at 527.768 kb on + strand, within GFF530 at 527.768 kb on + strand, within GFF530 at 527.768 kb on + strand, within GFF530 at 527.776 kb on - strand, within GFF530 at 527.776 kb on - strand, within GFF530 at 527.776 kb on - strand, within GFF530 at 527.776 kb on - strand, within GFF530 at 527.817 kb on + strand, within GFF530 at 527.825 kb on - strand, within GFF530 at 527.830 kb on - strand, within GFF530 at 527.831 kb on - strand, within GFF530 at 527.859 kb on - strand, within GFF530 at 527.877 kb on + strand, within GFF530 at 527.883 kb on - strand, within GFF530 at 527.883 kb on - strand, within GFF530 at 527.930 kb on + strand, within GFF530 at 527.932 kb on + strand, within GFF530 at 527.938 kb on - strand, within GFF530 at 527.944 kb on + strand, within GFF530 at 527.945 kb on - strand, within GFF530 at 527.965 kb on + strand, within GFF530 at 527.978 kb on - strand, within GFF530 at 528.007 kb on - strand, within GFF530 at 528.007 kb on - strand, within GFF530 at 528.099 kb on + strand, within GFF530 at 528.152 kb on - strand, within GFF530 at 528.170 kb on + strand, within GFF530 at 528.170 kb on + strand, within GFF530 at 528.178 kb on - strand, within GFF530 at 528.178 kb on - strand, within GFF530 at 528.178 kb on - strand, within GFF530 at 528.178 kb on - strand, within GFF530 at 528.178 kb on - strand, within GFF530 at 528.178 kb on - strand, within GFF530 at 528.216 kb on + strand at 528.258 kb on + strand at 528.268 kb on + strand at 528.308 kb on + strand at 528.308 kb on + strand at 528.318 kb on + strand at 528.343 kb on + strand at 528.351 kb on - strand at 528.364 kb on - strand at 528.401 kb on + strand at 528.459 kb on + strand, within GFF531 at 528.494 kb on + strand, within GFF531 at 528.512 kb on - strand, within GFF531 at 528.547 kb on + strand, within GFF531 at 528.547 kb on + strand, within GFF531 at 528.553 kb on + strand, within GFF531 at 528.555 kb on - strand, within GFF531 at 528.555 kb on - strand, within GFF531 at 528.555 kb on - strand, within GFF531 at 528.575 kb on + strand, within GFF531 at 528.575 kb on + strand, within GFF531 at 528.583 kb on - strand, within GFF531 at 528.583 kb on - strand, within GFF531 at 528.583 kb on - strand, within GFF531 at 528.597 kb on + strand, within GFF531 at 528.648 kb on - strand, within GFF531 at 528.685 kb on + strand, within GFF531 at 528.706 kb on + strand, within GFF531 at 528.749 kb on + strand, within GFF531 at 528.749 kb on + strand, within GFF531 at 528.757 kb on - strand, within GFF531 at 528.757 kb on - strand, within GFF531 at 528.775 kb on - strand, within GFF531 at 528.804 kb on + strand, within GFF531 at 528.841 kb on + strand, within GFF531 at 528.842 kb on + strand, within GFF531 at 528.848 kb on - strand, within GFF531 at 528.855 kb on - strand, within GFF531 at 528.885 kb on + strand, within GFF531 at 528.887 kb on + strand, within GFF531 at 528.889 kb on + strand, within GFF531 at 528.901 kb on - strand, within GFF531 at 528.910 kb on + strand, within GFF531 at 528.976 kb on - strand, within GFF531 at 528.978 kb on - strand, within GFF531 at 529.001 kb on + strand, within GFF531 at 529.001 kb on + strand, within GFF531 at 529.009 kb on - strand, within GFF531 at 529.021 kb on + strand, within GFF531 at 529.021 kb on + strand, within GFF531 at 529.021 kb on + strand, within GFF531 at 529.026 kb on + strand, within GFF531 at 529.029 kb on - strand, within GFF531 at 529.034 kb on - strand, within GFF531 at 529.034 kb on - strand, within GFF531 at 529.062 kb on - strand, within GFF531 at 529.076 kb on + strand, within GFF531 at 529.094 kb on + strand, within GFF531 at 529.095 kb on + strand, within GFF531 at 529.095 kb on + strand, within GFF531 at 529.095 kb on + strand, within GFF531 at 529.095 kb on + strand, within GFF531 at 529.095 kb on + strand, within GFF531 at 529.095 kb on + strand, within GFF531 at 529.095 kb on + strand, within GFF531 at 529.103 kb on - strand, within GFF531 at 529.103 kb on - strand, within GFF531 at 529.103 kb on - strand, within GFF531 at 529.103 kb on - strand, within GFF531 at 529.103 kb on - strand, within GFF531 at 529.103 kb on - strand, within GFF531 at 529.105 kb on + strand, within GFF531 at 529.105 kb on + strand, within GFF531 at 529.105 kb on + strand, within GFF531 at 529.113 kb on - strand, within GFF531 at 529.113 kb on - strand, within GFF531 at 529.113 kb on - strand, within GFF531 at 529.118 kb on + strand, within GFF531 at 529.126 kb on - strand, within GFF531 at 529.126 kb on - strand, within GFF531 at 529.127 kb on + strand, within GFF531 at 529.133 kb on - strand, within GFF531 at 529.160 kb on - strand, within GFF531 at 529.178 kb on + strand, within GFF531 at 529.181 kb on - strand, within GFF531 at 529.187 kb on - strand, within GFF531 at 529.192 kb on - strand, within GFF531 at 529.200 kb on + strand, within GFF531 at 529.200 kb on + strand, within GFF531 at 529.230 kb on + strand, within GFF531 at 529.230 kb on + strand, within GFF531 at 529.238 kb on - strand, within GFF531 at 529.238 kb on - strand, within GFF531 at 529.238 kb on - strand, within GFF531 at 529.238 kb on - strand, within GFF531 at 529.239 kb on - strand, within GFF531 at 529.262 kb on + strand, within GFF531 at 529.275 kb on + strand, within GFF531 at 529.286 kb on + strand, within GFF531
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with D-Glucose and Ammonium Sulfate remove 526,405 - GFF528 0.46 +0.3 526,405 - GFF528 0.46 +1.3 526,406 + GFF528 0.46 +0.7 526,437 - GFF528 0.48 -1.0 526,472 + GFF528 0.51 -0.3 526,472 + GFF528 0.51 -0.4 526,472 + GFF528 0.51 +0.9 526,482 + GFF528 0.52 -2.4 526,495 + GFF528 0.53 -0.6 526,501 + GFF528 0.54 -0.7 526,517 - GFF528 0.55 +0.8 526,519 + GFF528 0.55 +1.9 526,538 - GFF528 0.57 -0.8 526,602 - GFF528 0.63 -1.8 526,642 - GFF528 0.66 +0.4 526,652 - GFF528 0.67 -0.2 526,659 + GFF528 0.67 -1.4 526,667 - GFF528 0.68 +1.1 526,688 + GFF528 0.70 -0.3 526,722 - GFF528 0.73 +1.0 526,831 + GFF528 0.82 -1.4 526,878 - GFF528 0.86 +0.8 526,904 - GFF528 0.89 -0.3 526,938 + -1.1 526,938 + +1.4 526,938 + +0.6 526,938 + +0.4 526,943 + -2.2 526,943 + -1.6 526,946 - +0.3 526,946 - +0.2 526,946 - -0.7 526,946 - +0.8 526,951 - +0.5 526,951 - -1.0 526,951 - +1.4 526,956 + -2.4 526,963 - -1.4 526,964 - -0.7 526,964 - +0.3 526,964 - +1.9 526,966 + -1.4 526,974 - +2.0 527,032 - -0.2 527,036 + -1.3 527,044 - -0.4 527,044 - -0.5 527,106 + GFF529 0.22 +1.1 527,114 - GFF529 0.25 -1.0 527,114 - GFF529 0.25 -0.8 527,171 + GFF529 0.45 -1.5 527,197 + GFF529 0.54 -0.7 527,217 + GFF529 0.60 -0.5 527,217 + GFF529 0.60 +1.4 527,217 + GFF529 0.60 +0.3 527,225 - GFF529 0.63 +0.3 527,230 - GFF529 0.65 -0.1 527,263 + GFF529 0.76 -0.8 527,294 + GFF529 0.87 +0.1 527,300 - GFF529 0.89 -0.6 527,328 - -1.3 527,334 + +1.0 527,353 + -1.3 527,361 - -0.7 527,361 - -0.3 527,377 - +0.9 527,409 + +0.6 527,409 + -0.6 527,427 + -0.2 527,464 - GFF530 0.10 -1.7 527,481 + GFF530 0.12 -0.6 527,488 - GFF530 0.13 +0.1 527,489 - GFF530 0.13 +0.1 527,517 - GFF530 0.16 -1.5 527,526 + GFF530 0.17 -0.7 527,531 + GFF530 0.17 +0.6 527,534 + GFF530 0.18 +0.6 527,534 - GFF530 0.18 -0.3 527,540 - GFF530 0.18 -1.6 527,578 + GFF530 0.22 +0.1 527,578 + GFF530 0.22 +2.5 527,586 - GFF530 0.23 -0.9 527,601 - GFF530 0.25 -1.5 527,613 - GFF530 0.26 -0.5 527,627 + GFF530 0.28 -0.6 527,635 - GFF530 0.29 +0.2 527,693 + GFF530 0.35 +0.2 527,693 + GFF530 0.35 +0.3 527,701 - GFF530 0.36 +1.2 527,723 + GFF530 0.38 +1.1 527,766 + GFF530 0.43 -1.5 527,766 + GFF530 0.43 -0.8 527,768 + GFF530 0.43 -0.6 527,768 + GFF530 0.43 -0.5 527,768 + GFF530 0.43 -0.6 527,768 + GFF530 0.43 +1.1 527,776 - GFF530 0.44 +0.5 527,776 - GFF530 0.44 +0.4 527,776 - GFF530 0.44 -0.1 527,776 - GFF530 0.44 -1.1 527,817 + GFF530 0.48 -0.5 527,825 - GFF530 0.49 -0.6 527,830 - GFF530 0.50 +0.7 527,831 - GFF530 0.50 +0.8 527,859 - GFF530 0.53 -0.3 527,877 + GFF530 0.55 +0.7 527,883 - GFF530 0.55 +0.6 527,883 - GFF530 0.55 -0.6 527,930 + GFF530 0.60 +0.5 527,932 + GFF530 0.61 +1.0 527,938 - GFF530 0.61 -0.9 527,944 + GFF530 0.62 -1.8 527,945 - GFF530 0.62 -0.3 527,965 + GFF530 0.64 +0.2 527,978 - GFF530 0.66 -1.2 528,007 - GFF530 0.69 +0.1 528,007 - GFF530 0.69 -1.6 528,099 + GFF530 0.79 +0.3 528,152 - GFF530 0.84 -0.5 528,170 + GFF530 0.86 -0.5 528,170 + GFF530 0.86 +0.5 528,178 - GFF530 0.87 -0.2 528,178 - GFF530 0.87 -1.2 528,178 - GFF530 0.87 +0.8 528,178 - GFF530 0.87 -1.2 528,178 - GFF530 0.87 +1.1 528,178 - GFF530 0.87 +1.3 528,216 + -1.5 528,258 + -0.0 528,268 + +0.2 528,308 + -0.1 528,308 + +1.0 528,318 + +0.5 528,343 + -1.3 528,351 - -0.3 528,364 - +1.0 528,401 + +0.7 528,459 + GFF531 0.11 +0.7 528,494 + GFF531 0.13 -0.3 528,512 - GFF531 0.15 +0.4 528,547 + GFF531 0.17 -0.1 528,547 + GFF531 0.17 +0.2 528,553 + GFF531 0.18 +0.5 528,555 - GFF531 0.18 -0.3 528,555 - GFF531 0.18 +0.4 528,555 - GFF531 0.18 +0.6 528,575 + GFF531 0.19 -0.6 528,575 + GFF531 0.19 +0.2 528,583 - GFF531 0.20 +0.8 528,583 - GFF531 0.20 -0.0 528,583 - GFF531 0.20 -2.3 528,597 + GFF531 0.21 -0.3 528,648 - GFF531 0.25 +0.4 528,685 + GFF531 0.27 +0.2 528,706 + GFF531 0.29 +0.1 528,749 + GFF531 0.32 +0.1 528,749 + GFF531 0.32 -1.1 528,757 - GFF531 0.33 +0.5 528,757 - GFF531 0.33 -0.7 528,775 - GFF531 0.34 -2.3 528,804 + GFF531 0.36 +1.4 528,841 + GFF531 0.39 -0.4 528,842 + GFF531 0.39 -1.1 528,848 - GFF531 0.39 -0.7 528,855 - GFF531 0.40 -0.8 528,885 + GFF531 0.42 -0.2 528,887 + GFF531 0.42 +0.3 528,889 + GFF531 0.42 +0.6 528,901 - GFF531 0.43 +0.4 528,910 + GFF531 0.44 +0.2 528,976 - GFF531 0.49 -0.3 528,978 - GFF531 0.49 -0.9 529,001 + GFF531 0.51 -0.5 529,001 + GFF531 0.51 +0.9 529,009 - GFF531 0.51 -0.2 529,021 + GFF531 0.52 -2.2 529,021 + GFF531 0.52 -0.7 529,021 + GFF531 0.52 -1.3 529,026 + GFF531 0.52 +0.6 529,029 - GFF531 0.53 -1.7 529,034 - GFF531 0.53 +0.3 529,034 - GFF531 0.53 -1.7 529,062 - GFF531 0.55 +1.0 529,076 + GFF531 0.56 +1.2 529,094 + GFF531 0.57 -0.3 529,095 + GFF531 0.58 -0.2 529,095 + GFF531 0.58 +1.2 529,095 + GFF531 0.58 +1.2 529,095 + GFF531 0.58 +0.9 529,095 + GFF531 0.58 -1.5 529,095 + GFF531 0.58 -2.4 529,095 + GFF531 0.58 +0.1 529,103 - GFF531 0.58 -0.1 529,103 - GFF531 0.58 -0.5 529,103 - GFF531 0.58 -0.0 529,103 - GFF531 0.58 +0.8 529,103 - GFF531 0.58 +0.3 529,103 - GFF531 0.58 +2.4 529,105 + GFF531 0.58 +0.7 529,105 + GFF531 0.58 -0.8 529,105 + GFF531 0.58 +0.2 529,113 - GFF531 0.59 -0.4 529,113 - GFF531 0.59 +0.6 529,113 - GFF531 0.59 +1.0 529,118 + GFF531 0.59 +1.3 529,126 - GFF531 0.60 -1.7 529,126 - GFF531 0.60 -0.9 529,127 + GFF531 0.60 +0.8 529,133 - GFF531 0.60 -1.5 529,160 - GFF531 0.62 -1.0 529,178 + GFF531 0.64 -1.8 529,181 - GFF531 0.64 +0.4 529,187 - GFF531 0.64 +1.2 529,192 - GFF531 0.65 -0.0 529,200 + GFF531 0.65 -0.3 529,200 + GFF531 0.65 +0.1 529,230 + GFF531 0.67 -0.4 529,230 + GFF531 0.67 +0.2 529,238 - GFF531 0.68 -0.6 529,238 - GFF531 0.68 +0.5 529,238 - GFF531 0.68 -0.2 529,238 - GFF531 0.68 +1.2 529,239 - GFF531 0.68 -0.9 529,262 + GFF531 0.70 -0.0 529,275 + GFF531 0.71 -0.4 529,286 + GFF531 0.72 -0.2
Or see this region's nucleotide sequence